Click here to switch to the map view.

The map label for this gene is grpE [H]

Identifier: 197294600

GI number: 197294600

Start: 567018

End: 567686

Strand: Reverse

Name: grpE [H]

Synonym: PAa_0556

Alternate gene names: 197294600

Gene position: 567686-567018 (Counterclockwise)

Preceding gene: 197294601

Following gene: 197294599

Centisome position: 64.51

GC content: 26.91

Gene sequence:

>669_bases
ATGTTATTTGAAGAAAAAGAAGAAAAAAATAATTGTCAAGCTTGCGATGTTTGCGATGACAATGAAAAAAATGCAACCCC
AACCGAAGAAACAAAGAAAAAACTTAATTCGGATTGCGATTGCACTCATCAAGACAACACACAAACTGAAAAAAAACCAT
CATCAAAAACAATTGTTAAATTAAAAGAACAAATCAATAAATTAACCCTTCAATTGGAAGAACAAAAAAAAGCATTTGCT
GATGCTTCTTTGAAAAATCAAGCAGAATTAATTAATTTTAAAAAAAGATTGCAAAAGCAAAAAATACAAGAATTAAAATA
TGCTTCTAGCAACTTGATTTCTGATTTGTTAGTTCCTTTGGAACAATTAGAAAAAGCATTAGAAATTTCAACAGATAATG
AATTATTAAAAAAATATTTGTTAGGTTTTCAAATGATTCAACAACAAATTAAAAACATTTTGAAAGAAGAAGGAGTTGAA
GAAATTCAAGCCTTAAATGCTGTTTTTAATCCAGCATTGCACAATGCTTTAGAAAAAATTTCGGAACCAGAAAAACCAAA
CAAAACTAATTTAAAAGTTTTACAAAAAGGTTATTTATATAAAGAAAAAATTTTACGACCAGCAATGGTTCAAGTAAATG
AATGGAGTAATGAAGATGGCAAAAAGTAA

Upstream 100 bases:

>100_bases
TGAAATATCCTAAAATTATTCCCCTTTTAGAATATTTATCAATTCATTTATCAAAATTAAACCAGGAATAAATTAAAAAT
ATTTACGAAAGGGGCTAAAA

Downstream 100 bases:

>100_bases
TAAAATTATAGGAATTGACTTAGGAACAACTAATTCTTGTGTTGCTCTTATGGAAGGCGGAGAGGCCAAAGTTATTCCCA
ACGCTGAAGGCGGAAGAACA

Product: Molecular chaperone GrpE (heat shock protein)

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLFEEKEEKNNCQACDVCDDNEKNATPTEETKKKLNSDCDCTHQDNTQTEKKPSSKTIVKLKEQINKLTLQLEEQKKAFA
DASLKNQAELINFKKRLQKQKIQELKYASSNLISDLLVPLEQLEKALEISTDNELLKKYLLGFQMIQQQIKNILKEEGVE
EIQALNAVFNPALHNALEKISEPEKPNKTNLKVLQKGYLYKEKILRPAMVQVNEWSNEDGKK

Sequences:

>Translated_222_residues
MLFEEKEEKNNCQACDVCDDNEKNATPTEETKKKLNSDCDCTHQDNTQTEKKPSSKTIVKLKEQINKLTLQLEEQKKAFA
DASLKNQAELINFKKRLQKQKIQELKYASSNLISDLLVPLEQLEKALEISTDNELLKKYLLGFQMIQQQIKNILKEEGVE
EIQALNAVFNPALHNALEKISEPEKPNKTNLKVLQKGYLYKEKILRPAMVQVNEWSNEDGKK
>Mature_222_residues
MLFEEKEEKNNCQACDVCDDNEKNATPTEETKKKLNSDCDCTHQDNTQTEKKPSSKTIVKLKEQINKLTLQLEEQKKAFA
DASLKNQAELINFKKRLQKQKIQELKYASSNLISDLLVPLEQLEKALEISTDNELLKKYLLGFQMIQQQIKNILKEEGVE
EIQALNAVFNPALHNALEKISEPEKPNKTNLKVLQKGYLYKEKILRPAMVQVNEWSNEDGKK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=171, Percent_Identity=25.1461988304094, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 25592; Mature: 25592

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFEEKEEKNNCQACDVCDDNEKNATPTEETKKKLNSDCDCTHQDNTQTEKKPSSKTIVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCCCCHHHHHH
LKEQINKLTLQLEEQKKAFADASLKNQAELINFKKRLQKQKIQELKYASSNLISDLLVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLEKALEISTDNELLKKYLLGFQMIQQQIKNILKEEGVEEIQALNAVFNPALHNALEKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEPEKPNKTNLKVLQKGYLYKEKILRPAMVQVNEWSNEDGKK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLFEEKEEKNNCQACDVCDDNEKNATPTEETKKKLNSDCDCTHQDNTQTEKKPSSKTIVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHCCCCHHHHHH
LKEQINKLTLQLEEQKKAFADASLKNQAELINFKKRLQKQKIQELKYASSNLISDLLVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLEKALEISTDNELLKKYLLGFQMIQQQIKNILKEEGVEEIQALNAVFNPALHNALEKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEPEKPNKTNLKVLQKGYLYKEKILRPAMVQVNEWSNEDGKK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA