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The map label for this gene is tyrS

Identifier: 197294590

GI number: 197294590

Start: 553865

End: 555112

Strand: Reverse

Name: tyrS

Synonym: PAa_0546

Alternate gene names: 197294590

Gene position: 555112-553865 (Counterclockwise)

Preceding gene: 197294591

Following gene: 197294589

Centisome position: 63.08

GC content: 26.92

Gene sequence:

>1248_bases
ATGTCTTTTTATGAAGAATTAAAATGGCGTAATTTAATTAAAGATTCTAGTGATGAAAAAGAATTAAAAAATCTTTTAGA
TTGTAAAAAAATTAAATTTTATTGCGGTTTTGATCCTACTTCCGATTCTTTAACTGTGGGACATTTAGTTCAGTTAACAA
TGATGTCATTATTAGAAAAACAAGGACATTCTCCTTTTATTTTAGTTGGTGGAGCCACTGGTTTAATTGGTGATCCTAAA
GAGACAGAAGAAAGAAAATTATTATCTTTAGAAACATCTTTGCAAAACGCCAAGAGTCTTGAAACACAATTGAAAAAGAT
TTTATTTCAAAAAAAAATAACTTTTTTAAACAATTATGAATGGTTTTCTCAATTAGATATAATTACTTTTTTAAGAAAAT
ACGGCAAACTTTTTAATATTAATTATATGTTGAACAAACAAACAGTGGCCAAAAGATTGTCTTCAGGGATTTCTTTTACC
GAGTTTACTTACATGATTTTACAATCTTTAGATTTTCATCATTTGTATAAAAATTATGGTGTTCAATTGCAATTAGGCGG
TTCTGATCAATGGGGAAACATCACAAGTGGGTTAGAATTAATTAGAAAATTAGAAACAACAAGTCATGCTTTTGGTCTTA
GTACCCCACTTTTATTGAATGCTAATGGAACTAAATTTGGCAAGAGCGAACAAGATGTTTTATGGTTAGATCCTCAAAAA
ACATCTCCTTATAAAATTTATCAATATTTTTTGAATCTTGCTGATGAAGAAGTTGTTAATTGCTTAAAACAACTTACCTT
AATCCCCAAAGAAGAGATTTTAGAATTAGAAAAAGCCACCCTTCAAACTCCTCAAAAACGTTTAGCTCAAAAAGCTTTAG
CGAACAACATTGTTAATTTAATTTATTCTCAAAAAGTTTTGCAAGAATGTCACAAAACTAATGAAGTTTTATTTTTAAAT
AAAAAGAAAGAAAGTTTTCAAGAAGCAGATTTTACCTTATTAAATAAAACTTTATTTTCATATGAAACATCATTAGTTTC
TATTCCTTTATCTGAGGCTTTAGTAAAAACCCAATTAACTACTTCCAAAAGGGAAGCGCGCGAATTTATCCAAAGAGGAA
GCATTCAAATTTTTAATGAAAAAATCAAAAATCCTGATTATTTAATTGACAAAAAAAACACTTTATTTAATAAATACGTT
CTTTTAAAAAAAGGAAAAAAAAACAACTCTTTAATTATTTTGAACTAA

Upstream 100 bases:

>100_bases
CTAAAGCTGCGAGAATTAAGGAAAAAAGATAAATTATTTTTTCTTTAAAAAAATAATATTTTAGAAAAATTCTTTTTTAA
ATAAAAATAGGAGTTTGAAA

Downstream 100 bases:

>100_bases
TTTTATAATAAAATGGAGGCACAAATAGTGTTAAAAAACCCTTTGCGATCGCTTTTTCCCATTTTTAAAGCCAACCCCAA
ACTTGTTTATTTTGATTCTG

Product: Tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSFYEELKWRNLIKDSSDEKELKNLLDCKKIKFYCGFDPTSDSLTVGHLVQLTMMSLLEKQGHSPFILVGGATGLIGDPK
ETEERKLLSLETSLQNAKSLETQLKKILFQKKITFLNNYEWFSQLDIITFLRKYGKLFNINYMLNKQTVAKRLSSGISFT
EFTYMILQSLDFHHLYKNYGVQLQLGGSDQWGNITSGLELIRKLETTSHAFGLSTPLLLNANGTKFGKSEQDVLWLDPQK
TSPYKIYQYFLNLADEEVVNCLKQLTLIPKEEILELEKATLQTPQKRLAQKALANNIVNLIYSQKVLQECHKTNEVLFLN
KKKESFQEADFTLLNKTLFSYETSLVSIPLSEALVKTQLTTSKREAREFIQRGSIQIFNEKIKNPDYLIDKKNTLFNKYV
LLKKGKKNNSLIILN

Sequences:

>Translated_415_residues
MSFYEELKWRNLIKDSSDEKELKNLLDCKKIKFYCGFDPTSDSLTVGHLVQLTMMSLLEKQGHSPFILVGGATGLIGDPK
ETEERKLLSLETSLQNAKSLETQLKKILFQKKITFLNNYEWFSQLDIITFLRKYGKLFNINYMLNKQTVAKRLSSGISFT
EFTYMILQSLDFHHLYKNYGVQLQLGGSDQWGNITSGLELIRKLETTSHAFGLSTPLLLNANGTKFGKSEQDVLWLDPQK
TSPYKIYQYFLNLADEEVVNCLKQLTLIPKEEILELEKATLQTPQKRLAQKALANNIVNLIYSQKVLQECHKTNEVLFLN
KKKESFQEADFTLLNKTLFSYETSLVSIPLSEALVKTQLTTSKREAREFIQRGSIQIFNEKIKNPDYLIDKKNTLFNKYV
LLKKGKKNNSLIILN
>Mature_414_residues
SFYEELKWRNLIKDSSDEKELKNLLDCKKIKFYCGFDPTSDSLTVGHLVQLTMMSLLEKQGHSPFILVGGATGLIGDPKE
TEERKLLSLETSLQNAKSLETQLKKILFQKKITFLNNYEWFSQLDIITFLRKYGKLFNINYMLNKQTVAKRLSSGISFTE
FTYMILQSLDFHHLYKNYGVQLQLGGSDQWGNITSGLELIRKLETTSHAFGLSTPLLLNANGTKFGKSEQDVLWLDPQKT
SPYKIYQYFLNLADEEVVNCLKQLTLIPKEEILELEKATLQTPQKRLAQKALANNIVNLIYSQKVLQECHKTNEVLFLNK
KKESFQEADFTLLNKTLFSYETSLVSIPLSEALVKTQLTTSKREAREFIQRGSIQIFNEKIKNPDYLIDKKNTLFNKYVL
LKKGKKNNSLIILN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=439, Percent_Identity=34.3963553530752, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1787925, Length=416, Percent_Identity=42.3076923076923, Blast_Score=325, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI25145802, Length=369, Percent_Identity=31.4363143631436, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=308, Percent_Identity=29.8701298701299, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325160, Length=435, Percent_Identity=31.0344827586207, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI20130383, Length=425, Percent_Identity=33.4117647058824, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PHYAS (B1VAA7)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799131.1
- ProteinModelPortal:   B1VAA7
- SMR:   B1VAA7
- GeneID:   6798825
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG288125
- OMA:   HEVIILL
- ProtClustDB:   PRK05912
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47848; Mature: 47717

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 164-164 BINDING 168-168 BINDING 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFYEELKWRNLIKDSSDEKELKNLLDCKKIKFYCGFDPTSDSLTVGHLVQLTMMSLLEK
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QGHSPFILVGGATGLIGDPKETEERKLLSLETSLQNAKSLETQLKKILFQKKITFLNNYE
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
WFSQLDIITFLRKYGKLFNINYMLNKQTVAKRLSSGISFTEFTYMILQSLDFHHLYKNYG
HHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCC
VQLQLGGSDQWGNITSGLELIRKLETTSHAFGLSTPLLLNANGTKFGKSEQDVLWLDPQK
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCC
TSPYKIYQYFLNLADEEVVNCLKQLTLIPKEEILELEKATLQTPQKRLAQKALANNIVNL
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IYSQKVLQECHKTNEVLFLNKKKESFQEADFTLLNKTLFSYETSLVSIPLSEALVKTQLT
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
TSKREAREFIQRGSIQIFNEKIKNPDYLIDKKNTLFNKYVLLKKGKKNNSLIILN
HHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCHHHHHHEEEEECCCCCCCEEEEC
>Mature Secondary Structure 
SFYEELKWRNLIKDSSDEKELKNLLDCKKIKFYCGFDPTSDSLTVGHLVQLTMMSLLEK
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QGHSPFILVGGATGLIGDPKETEERKLLSLETSLQNAKSLETQLKKILFQKKITFLNNYE
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
WFSQLDIITFLRKYGKLFNINYMLNKQTVAKRLSSGISFTEFTYMILQSLDFHHLYKNYG
HHHHHHHHHHHHHCCCEEEEEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCC
VQLQLGGSDQWGNITSGLELIRKLETTSHAFGLSTPLLLNANGTKFGKSEQDVLWLDPQK
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCC
TSPYKIYQYFLNLADEEVVNCLKQLTLIPKEEILELEKATLQTPQKRLAQKALANNIVNL
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
IYSQKVLQECHKTNEVLFLNKKKESFQEADFTLLNKTLFSYETSLVSIPLSEALVKTQLT
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHH
TSKREAREFIQRGSIQIFNEKIKNPDYLIDKKNTLFNKYVLLKKGKKNNSLIILN
HHHHHHHHHHHCCCCEEEHHHCCCCCEEEECCHHHHHHEEEEECCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA