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The map label for this gene is pheS

Identifier: 197294576

GI number: 197294576

Start: 529643

End: 530674

Strand: Reverse

Name: pheS

Synonym: PAa_0532

Alternate gene names: 197294576

Gene position: 530674-529643 (Counterclockwise)

Preceding gene: 197294577

Following gene: 197294575

Centisome position: 60.31

GC content: 27.52

Gene sequence:

>1032_bases
ATGCATAATAAAATCAATCTTTTAAAAGAAAAATTTGAACATGATTTAGCAAAAAACAAAAATAGTTTAGAAAATTTAAT
TTATTTAGAACAAAAATTTTTAGGAAAAAAAAGCTTTTTGCAAGAATTAAACCAAACTTTAAAAAATATTCCTTCAGAAA
AAAAACCTGCAATCGGAAAATTAATCAATGAATTTAAAAAAACAATTATTTTATCTTTGCAAAAAGAAAAACAATATTTA
AAAACGCAAAAAATGAACTTAGAGTTAATGCAAGAAAGTGTTGATGTGAATTTACCGGGTTTTAATTTTTCGACAGGAAG
CATTCACCCTCTTTACCAAATTATCGAACAATTAGAAGATTTATTTTTGTCTTTAGGATACGAAATTAAAGATGGCAATG
AAATTGAAAGTGATTTTTATAATTTTGAAATGTTAAATATTGGCAAAAACCATCCCGCCCGAGCAATGCAAGATTCTTTT
TATATTAACCCCCAAAAACTTTTAAGAACCCACACTTCCAATATTCAAGTTAAAGAAATGTTGGCTAATGAAGGGAAACC
ATTGAAAATTATTTCTTCTGGTAAAGTTTTTCGCAAAGATAACGATGATGCCACTCACAGTCATCAATTTATGCAATTAG
AAGGGTTGGTTATCGATCAAAAGATTAATTTTTTGAATTTAAAAGAAACCATTTTAACAATCATTAAAGAACTTTTTGGC
GATTCTCAAGAAATAAATATAAGACCTTCTTATTTCCCCTTTACAGAACCAAGCATTGAAGTTGATTTAGTAATTCGCAA
AAAAGATAACAGTAAAGAATATTTAGAAATTTTGGGAGCGGGTTTGGTTCATCCCCAAGTTTTGTTGAATGCTAATTATA
ATCCCGAAAAATATCAAGGTTTTGCCTTCGGAATTGGCATCGAAAGAATTGCTATGATCAAATATCAAATTGAAAATATT
CGTCATTTTTATCAAAACGATATCCGCTTTTTAAAACAATTTTCAAAGAAGGTTAAACATGAAAATTCTTGA

Upstream 100 bases:

>100_bases
CTTTTTAGGAGCTACTTTGGCAATGTGGCTTTCTTTTTTAACTGAAGGGAATATTATTTCTTGGATATATCGGCTAAATT
CTTAAAAGAAAGGAAATATT

Downstream 100 bases:

>100_bases
ACCCATTTTAAAAAAACATTTTTCGAATTTACCCAAAACTAATAAACTTTTTGATTTGATTAATAATTATATTAATGAAG
TCGAAACTTTGCGGCCTATT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MHNKINLLKEKFEHDLAKNKNSLENLIYLEQKFLGKKSFLQELNQTLKNIPSEKKPAIGKLINEFKKTIILSLQKEKQYL
KTQKMNLELMQESVDVNLPGFNFSTGSIHPLYQIIEQLEDLFLSLGYEIKDGNEIESDFYNFEMLNIGKNHPARAMQDSF
YINPQKLLRTHTSNIQVKEMLANEGKPLKIISSGKVFRKDNDDATHSHQFMQLEGLVIDQKINFLNLKETILTIIKELFG
DSQEINIRPSYFPFTEPSIEVDLVIRKKDNSKEYLEILGAGLVHPQVLLNANYNPEKYQGFAFGIGIERIAMIKYQIENI
RHFYQNDIRFLKQFSKKVKHENS

Sequences:

>Translated_343_residues
MHNKINLLKEKFEHDLAKNKNSLENLIYLEQKFLGKKSFLQELNQTLKNIPSEKKPAIGKLINEFKKTIILSLQKEKQYL
KTQKMNLELMQESVDVNLPGFNFSTGSIHPLYQIIEQLEDLFLSLGYEIKDGNEIESDFYNFEMLNIGKNHPARAMQDSF
YINPQKLLRTHTSNIQVKEMLANEGKPLKIISSGKVFRKDNDDATHSHQFMQLEGLVIDQKINFLNLKETILTIIKELFG
DSQEINIRPSYFPFTEPSIEVDLVIRKKDNSKEYLEILGAGLVHPQVLLNANYNPEKYQGFAFGIGIERIAMIKYQIENI
RHFYQNDIRFLKQFSKKVKHENS
>Mature_343_residues
MHNKINLLKEKFEHDLAKNKNSLENLIYLEQKFLGKKSFLQELNQTLKNIPSEKKPAIGKLINEFKKTIILSLQKEKQYL
KTQKMNLELMQESVDVNLPGFNFSTGSIHPLYQIIEQLEDLFLSLGYEIKDGNEIESDFYNFEMLNIGKNHPARAMQDSF
YINPQKLLRTHTSNIQVKEMLANEGKPLKIISSGKVFRKDNDDATHSHQFMQLEGLVIDQKINFLNLKETILTIIKELFG
DSQEINIRPSYFPFTEPSIEVDLVIRKKDNSKEYLEILGAGLVHPQVLLNANYNPEKYQGFAFGIGIERIAMIKYQIENI
RHFYQNDIRFLKQFSKKVKHENS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=280, Percent_Identity=30.7142857142857, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI4758340, Length=271, Percent_Identity=30.2583025830258, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1788007, Length=318, Percent_Identity=43.3962264150943, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17508957, Length=270, Percent_Identity=29.2592592592593, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=270, Percent_Identity=29.2592592592593, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=249, Percent_Identity=30.1204819277108, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=270, Percent_Identity=30, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=126, Percent_Identity=36.5079365079365, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18858079, Length=263, Percent_Identity=31.5589353612167, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137424, Length=290, Percent_Identity=29.6551724137931, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PHYAS (B1VA93)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799117.1
- ProteinModelPortal:   B1VA93
- SMR:   B1VA93
- GeneID:   6799273
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40000; Mature: 40000

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNKINLLKEKFEHDLAKNKNSLENLIYLEQKFLGKKSFLQELNQTLKNIPSEKKPAIGK
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHH
LINEFKKTIILSLQKEKQYLKTQKMNLELMQESVDVNLPGFNFSTGSIHPLYQIIEQLED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LFLSLGYEIKDGNEIESDFYNFEMLNIGKNHPARAMQDSFYINPQKLLRTHTSNIQVKEM
HHHHCCCEECCCCCHHHHHHCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHH
LANEGKPLKIISSGKVFRKDNDDATHSHQFMQLEGLVIDQKINFLNLKETILTIIKELFG
HHCCCCCEEEEECCCEEECCCCCCHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHHHCC
DSQEINIRPSYFPFTEPSIEVDLVIRKKDNSKEYLEILGAGLVHPQVLLNANYNPEKYQG
CCCEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCHHHHCC
FAFGIGIERIAMIKYQIENIRHFYQNDIRFLKQFSKKVKHENS
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHNKINLLKEKFEHDLAKNKNSLENLIYLEQKFLGKKSFLQELNQTLKNIPSEKKPAIGK
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHH
LINEFKKTIILSLQKEKQYLKTQKMNLELMQESVDVNLPGFNFSTGSIHPLYQIIEQLED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
LFLSLGYEIKDGNEIESDFYNFEMLNIGKNHPARAMQDSFYINPQKLLRTHTSNIQVKEM
HHHHCCCEECCCCCHHHHHHCEEEEECCCCCCCHHHHCCCCCCHHHHHHHHCCCCCHHHH
LANEGKPLKIISSGKVFRKDNDDATHSHQFMQLEGLVIDQKINFLNLKETILTIIKELFG
HHCCCCCEEEEECCCEEECCCCCCHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHHHCC
DSQEINIRPSYFPFTEPSIEVDLVIRKKDNSKEYLEILGAGLVHPQVLLNANYNPEKYQG
CCCEEEECCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCCHHHHCC
FAFGIGIERIAMIKYQIENIRHFYQNDIRFLKQFSKKVKHENS
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA