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The map label for this gene is pheT [H]

Identifier: 197294575

GI number: 197294575

Start: 527227

End: 529656

Strand: Reverse

Name: pheT [H]

Synonym: PAa_0531

Alternate gene names: 197294575

Gene position: 529656-527227 (Counterclockwise)

Preceding gene: 197294576

Following gene: 197294574

Centisome position: 60.19

GC content: 27.82

Gene sequence:

>2430_bases
ATGAAAATTCTTGAACCCATTTTAAAAAAACATTTTTCGAATTTACCCAAAACTAATAAACTTTTTGATTTGATTAATAA
TTATATTAATGAAGTCGAAACTTTGCGGCCTATTTCTCAAAATACTAATTTAGTTGTTGGAAAAATTCTTGAATATCAAA
AAATTACTGGCTCACAAAAACTCAACTTAGTTACAGTTGACATAGGAACAAGAATTTTAAAAATTGTTTGTGGCGCCACT
AATTTAGATAATAACGGTAAAGTAATTGTTGCTTTAGAAGGTTCTTTTTTAGAAGGTTTTCAAACAACTCTTAAAAATAA
AAAAATTTATGGTGTTTTTTCTGAAGGTATGCTTTGTGCTTTAGAAGAATTAGGAATTGATTCTTCCATTTTAACTTCAG
AAGAAAAAACCGGCATTTATTTTTTTCAAGACACGCAAGATGAAATAAAATTAGGTAGTAACGCTTTAATTCCTTTAGGT
TTGGCGGGTTTTTTTTTAGAATTAGGAGTCACTCCTAATCGTGCCGATCTTTTATCTTATTTTGGTTTTGCAAAGGATTT
AGCTGCTGTTTTAAAAAGTATATCTTTTTCTGATAATACTTTTACTGATGATTGTGGTGATTTTTCCAAAGACAATCAAA
GTTTAGAAAATGTAGAAAACTTTTTTGCTACTAAAAAAACTTCTCCTTTAAAAGTAAACCTTGAAAGCAATGCTTGTTTA
GAATATCATGCTTGTATATTAGAAAATATAACAATCAAACCCTCTCCTTTGTGGTTAAGAAATACACTTTTACATTCAGA
CATAAAACCAATCAATAATGTTGTTGACGTTACAAACTTAATCATGCTTGAATACGGAATTCCTTTGCATGCTTTTGATT
CTTCAAATATTAAAGAAATTAAAGTAAGACAAGCCCTACCACAAGAAAAAATTACAACTTTAAATGAAAACGCCTTTGAA
TTAGACTCTCGTGATTTGGTTATTACAGACGGAACAACTCCCATTGCTTTGGCAGGTATCATAGGTCTTTTGGAAAGTGG
TATCAAAAATGACCCTAAAAACATCATTTTAGAATGTGCTTATTTTAAACCAGAAAGCATTTCCCAAACTAGCCAAAAAT
TAAAAATTAAAACTATGAGTTCTTTGCGTTTCGAAAGAGGAGTAAACCCCGAATTAATCCCTTTGGCTTTAAAAAAAGCA
TGCGAACTTTTAATCAAACTAGCTGATGCAACAATAACTTATCAACCGATAAATAAAAAAAGCGAAGTCATTAAGCCAAA
AGTAATTGATTTAAATTTAGATGATGTCAAGAAAAAAACGGGCATTACGTTTTTATTATCTCAAACTAAAAACTGGCTAT
TAAATCTAGGTTATCAAATTTTGAATGTAGACGGCAACAATTTAAAAGTAATCCCTCCTTTTCGTAGATATGATATTAAA
ATTAAAGAAAATGTTATTGCTGATTTAGTCCGTCTTTACGGCTGCAAAAATTTACTTGTTAACAAAAAAAACCAAAATAA
ACAAATTAAAATGACTTTAAAACAAAAAAACCTCAGGGAATTAAAAAAAATCTTGGTTAATTTAGGTTTTTACGAAACAG
TAACTTATAGTTTAATTAGTCAAGAAATGTTTGAATGTTTTAATTATCAAAAAGAAATAATCCAAATATTAAACCCTTTA
TCGCAAGATAAAGTTATTTTAAGACAAAGTTTATTAAGTGGCTTAATTGAAGTAATGAGCTACCAACACAAAAGAAAAAA
TTTTGATAATGCTTTTTTTGAAATCAGTAAAGTGTATCACCCAGAAAAAGAAAAATTAAGCCTAGGTTTGATTTTAAGTG
GTAAAATATTGACTGGCGGTTGGCAAAAACAAGATATCACAAGTTCTTTTTTTGTTTTAAAAGGTGTTGTGGAAAAAATA
AGTTCTTTTTTAGGCGTTGATCTAACCTATCAAAAAAGTTCTTCTAATCTTAATCTTCATCCAGGGATTCAAGCAGGAAT
TTTATATCAAAATGAAATAATTGGAAAAATAGGAAAAATACATCCTAATTTAAACGAAAAACATCATTTAAAAGACAGTT
TTGTTTGTGAAATTTTGTTATCAGACCAATTATTAAACAAAAACTATAAAATTGTATATCATCCTATTAGCAAATTCCCT
GTTGTTACCCGCGATTTGTCTTTTTTAATTGCTTCTCAATATGCTTTTTTAGATGTTCAAAAAACTATTCAAAAACATTC
CTTCCTTGATTTAATTGATTGTCAACTTTTTGATGTTTATCAAATTGATAATGACAAAAAAGAGCTATCTTTAGCTTTTC
GCTTATCTTTTTCCAGCCCTTTTAATAATTTAGAAAAAGAAATAATTGACCAAAGAATGAATATTATCGTTGACAATTTA
ATTCAAAATTATCAAATTAAAATAAGGTAG

Upstream 100 bases:

>100_bases
ATCGAAAGAATTGCTATGATCAAATATCAAATTGAAAATATTCGTCATTTTTATCAAAACGATATCCGCTTTTTAAAACA
ATTTTCAAAGAAGGTTAAAC

Downstream 100 bases:

>100_bases
TTTTTTTTGGTTTTTTCCGCTTCTTGATCTATCAAAATCTAAACTTTAATTAAAAAATTATTTACCCTATATAATCACTA
AATCTCAAATAAATTAAAAA

Product: Phenylalanyl-tRNA synthetase beta-chain

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MKILEPILKKHFSNLPKTNKLFDLINNYINEVETLRPISQNTNLVVGKILEYQKITGSQKLNLVTVDIGTRILKIVCGAT
NLDNNGKVIVALEGSFLEGFQTTLKNKKIYGVFSEGMLCALEELGIDSSILTSEEKTGIYFFQDTQDEIKLGSNALIPLG
LAGFFLELGVTPNRADLLSYFGFAKDLAAVLKSISFSDNTFTDDCGDFSKDNQSLENVENFFATKKTSPLKVNLESNACL
EYHACILENITIKPSPLWLRNTLLHSDIKPINNVVDVTNLIMLEYGIPLHAFDSSNIKEIKVRQALPQEKITTLNENAFE
LDSRDLVITDGTTPIALAGIIGLLESGIKNDPKNIILECAYFKPESISQTSQKLKIKTMSSLRFERGVNPELIPLALKKA
CELLIKLADATITYQPINKKSEVIKPKVIDLNLDDVKKKTGITFLLSQTKNWLLNLGYQILNVDGNNLKVIPPFRRYDIK
IKENVIADLVRLYGCKNLLVNKKNQNKQIKMTLKQKNLRELKKILVNLGFYETVTYSLISQEMFECFNYQKEIIQILNPL
SQDKVILRQSLLSGLIEVMSYQHKRKNFDNAFFEISKVYHPEKEKLSLGLILSGKILTGGWQKQDITSSFFVLKGVVEKI
SSFLGVDLTYQKSSSNLNLHPGIQAGILYQNEIIGKIGKIHPNLNEKHHLKDSFVCEILLSDQLLNKNYKIVYHPISKFP
VVTRDLSFLIASQYAFLDVQKTIQKHSFLDLIDCQLFDVYQIDNDKKELSLAFRLSFSSPFNNLEKEIIDQRMNIIVDNL
IQNYQIKIR

Sequences:

>Translated_809_residues
MKILEPILKKHFSNLPKTNKLFDLINNYINEVETLRPISQNTNLVVGKILEYQKITGSQKLNLVTVDIGTRILKIVCGAT
NLDNNGKVIVALEGSFLEGFQTTLKNKKIYGVFSEGMLCALEELGIDSSILTSEEKTGIYFFQDTQDEIKLGSNALIPLG
LAGFFLELGVTPNRADLLSYFGFAKDLAAVLKSISFSDNTFTDDCGDFSKDNQSLENVENFFATKKTSPLKVNLESNACL
EYHACILENITIKPSPLWLRNTLLHSDIKPINNVVDVTNLIMLEYGIPLHAFDSSNIKEIKVRQALPQEKITTLNENAFE
LDSRDLVITDGTTPIALAGIIGLLESGIKNDPKNIILECAYFKPESISQTSQKLKIKTMSSLRFERGVNPELIPLALKKA
CELLIKLADATITYQPINKKSEVIKPKVIDLNLDDVKKKTGITFLLSQTKNWLLNLGYQILNVDGNNLKVIPPFRRYDIK
IKENVIADLVRLYGCKNLLVNKKNQNKQIKMTLKQKNLRELKKILVNLGFYETVTYSLISQEMFECFNYQKEIIQILNPL
SQDKVILRQSLLSGLIEVMSYQHKRKNFDNAFFEISKVYHPEKEKLSLGLILSGKILTGGWQKQDITSSFFVLKGVVEKI
SSFLGVDLTYQKSSSNLNLHPGIQAGILYQNEIIGKIGKIHPNLNEKHHLKDSFVCEILLSDQLLNKNYKIVYHPISKFP
VVTRDLSFLIASQYAFLDVQKTIQKHSFLDLIDCQLFDVYQIDNDKKELSLAFRLSFSSPFNNLEKEIIDQRMNIIVDNL
IQNYQIKIR
>Mature_809_residues
MKILEPILKKHFSNLPKTNKLFDLINNYINEVETLRPISQNTNLVVGKILEYQKITGSQKLNLVTVDIGTRILKIVCGAT
NLDNNGKVIVALEGSFLEGFQTTLKNKKIYGVFSEGMLCALEELGIDSSILTSEEKTGIYFFQDTQDEIKLGSNALIPLG
LAGFFLELGVTPNRADLLSYFGFAKDLAAVLKSISFSDNTFTDDCGDFSKDNQSLENVENFFATKKTSPLKVNLESNACL
EYHACILENITIKPSPLWLRNTLLHSDIKPINNVVDVTNLIMLEYGIPLHAFDSSNIKEIKVRQALPQEKITTLNENAFE
LDSRDLVITDGTTPIALAGIIGLLESGIKNDPKNIILECAYFKPESISQTSQKLKIKTMSSLRFERGVNPELIPLALKKA
CELLIKLADATITYQPINKKSEVIKPKVIDLNLDDVKKKTGITFLLSQTKNWLLNLGYQILNVDGNNLKVIPPFRRYDIK
IKENVIADLVRLYGCKNLLVNKKNQNKQIKMTLKQKNLRELKKILVNLGFYETVTYSLISQEMFECFNYQKEIIQILNPL
SQDKVILRQSLLSGLIEVMSYQHKRKNFDNAFFEISKVYHPEKEKLSLGLILSGKILTGGWQKQDITSSFFVLKGVVEKI
SSFLGVDLTYQKSSSNLNLHPGIQAGILYQNEIIGKIGKIHPNLNEKHHLKDSFVCEILLSDQLLNKNYKIVYHPISKFP
VVTRDLSFLIASQYAFLDVQKTIQKHSFLDLIDCQLFDVYQIDNDKKELSLAFRLSFSSPFNNLEKEIIDQRMNIIVDNL
IQNYQIKIR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=772, Percent_Identity=29.1450777202073, Blast_Score=285, Evalue=9e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 91741; Mature: 91741

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILEPILKKHFSNLPKTNKLFDLINNYINEVETLRPISQNTNLVVGKILEYQKITGSQK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCC
LNLVTVDIGTRILKIVCGATNLDNNGKVIVALEGSFLEGFQTTLKNKKIYGVFSEGMLCA
EEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCHHHH
LEELGIDSSILTSEEKTGIYFFQDTQDEIKLGSNALIPLGLAGFFLELGVTPNRADLLSY
HHHCCCCHHHCCCCCCCCEEEEECCHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHHHH
FGFAKDLAAVLKSISFSDNTFTDDCGDFSKDNQSLENVENFFATKKTSPLKVNLESNACL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHH
EYHACILENITIKPSPLWLRNTLLHSDIKPINNVVDVTNLIMLEYGIPLHAFDSSNIKEI
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCEEECCCCCCHHH
KVRQALPQEKITTLNENAFELDSRDLVITDGTTPIALAGIIGLLESGIKNDPKNIILECA
HHHHHCCHHHHHHCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
YFKPESISQTSQKLKIKTMSSLRFERGVNPELIPLALKKACELLIKLADATITYQPINKK
ECCCCCHHHHHHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCC
SEVIKPKVIDLNLDDVKKKTGITFLLSQTKNWLLNLGYQILNVDGNNLKVIPPFRRYDIK
CCCCCCEEEECCHHHHHHHCCEEEEEHHHHHHHHHCCEEEEEECCCCEEEECCHHHEEEE
IKENVIADLVRLYGCKNLLVNKKNQNKQIKMTLKQKNLRELKKILVNLGFYETVTYSLIS
EHHHHHHHHHHHHCCHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHH
QEMFECFNYQKEIIQILNPLSQDKVILRQSLLSGLIEVMSYQHKRKNFDNAFFEISKVYH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PEKEKLSLGLILSGKILTGGWQKQDITSSFFVLKGVVEKISSFLGVDLTYQKSSSNLNLH
CCHHHEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEC
PGIQAGILYQNEIIGKIGKIHPNLNEKHHLKDSFVCEILLSDQLLNKNYKIVYHPISKFP
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VVTRDLSFLIASQYAFLDVQKTIQKHSFLDLIDCQLFDVYQIDNDKKELSLAFRLSFSSP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHEEEEEEEEECCCC
FNNLEKEIIDQRMNIIVDNLIQNYQIKIR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure
MKILEPILKKHFSNLPKTNKLFDLINNYINEVETLRPISQNTNLVVGKILEYQKITGSQK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCCC
LNLVTVDIGTRILKIVCGATNLDNNGKVIVALEGSFLEGFQTTLKNKKIYGVFSEGMLCA
EEEEEEEHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCCHHHH
LEELGIDSSILTSEEKTGIYFFQDTQDEIKLGSNALIPLGLAGFFLELGVTPNRADLLSY
HHHCCCCHHHCCCCCCCCEEEEECCHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHHHHH
FGFAKDLAAVLKSISFSDNTFTDDCGDFSKDNQSLENVENFFATKKTSPLKVNLESNACL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCHH
EYHACILENITIKPSPLWLRNTLLHSDIKPINNVVDVTNLIMLEYGIPLHAFDSSNIKEI
HHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHEECCCCEEECCCCCCHHH
KVRQALPQEKITTLNENAFELDSRDLVITDGTTPIALAGIIGLLESGIKNDPKNIILECA
HHHHHCCHHHHHHCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEE
YFKPESISQTSQKLKIKTMSSLRFERGVNPELIPLALKKACELLIKLADATITYQPINKK
ECCCCCHHHHHHHEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCC
SEVIKPKVIDLNLDDVKKKTGITFLLSQTKNWLLNLGYQILNVDGNNLKVIPPFRRYDIK
CCCCCCEEEECCHHHHHHHCCEEEEEHHHHHHHHHCCEEEEEECCCCEEEECCHHHEEEE
IKENVIADLVRLYGCKNLLVNKKNQNKQIKMTLKQKNLRELKKILVNLGFYETVTYSLIS
EHHHHHHHHHHHHCCHHHEECCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHH
QEMFECFNYQKEIIQILNPLSQDKVILRQSLLSGLIEVMSYQHKRKNFDNAFFEISKVYH
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
PEKEKLSLGLILSGKILTGGWQKQDITSSFFVLKGVVEKISSFLGVDLTYQKSSSNLNLH
CCHHHEEEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEC
PGIQAGILYQNEIIGKIGKIHPNLNEKHHLKDSFVCEILLSDQLLNKNYKIVYHPISKFP
CCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCC
VVTRDLSFLIASQYAFLDVQKTIQKHSFLDLIDCQLFDVYQIDNDKKELSLAFRLSFSSP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCEEEEEEECCCCHHEEEEEEEEECCCC
FNNLEKEIIDQRMNIIVDNLIQNYQIKIR
HHHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA