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The map label for this gene is greA [H]

Identifier: 197294551

GI number: 197294551

Start: 499080

End: 499550

Strand: Reverse

Name: greA [H]

Synonym: PAa_0506

Alternate gene names: 197294551

Gene position: 499550-499080 (Counterclockwise)

Preceding gene: 197294552

Following gene: 197294550

Centisome position: 56.77

GC content: 27.6

Gene sequence:

>471_bases
ATGAAAAATAAAATTTATGAACTAACCCAACCAGGAATTTTAAAATTAAAAAAAGAATTGACTTTTTTAAAAGAAGTTAA
AAGAAAAGAAAATTTAGAAGCTTTAAAAGAAGCGCGCGAACAAGGAGATTTATCAGAAAATGCCGATTACGACGCTGCGC
GTAACGAACAGGCTTTAATTGAATATCGCATTACAGAAATTCAAAATATTTTAAAAAATGTTCGTGTTGTAAAAGTTTCT
AAAGATAAAGAAATAAATATTGGAAAAAAAGTATCTTTAAAAATGCTTAATTCAGGAACTGAAGAAGAATTTGAATTAGT
AAGTACTTTGGAATCAGATCCATTCAGTAATAAAATCTCTATTGAATCCCCTTTAGGAAAAAAAATTAAGGGACATGAGA
TAGGAGATGTTGTCTTTATAACTAGCGAAACGGGTACGAAATTTAAGGTAAAAATTTTAGATGTTAAATAA

Upstream 100 bases:

>100_bases
TCCAGATTTTCAAACTGTTTTTAAAAATATTGATGATGGTTTAAGTATTTCTTATAAAATAACAGATAAAATAAAAATAA
ATTAAAAGAAAAGGTTTTTT

Downstream 100 bases:

>100_bases
AAAAAAATATATACCTCAATATTATTTCCCTTCAGTTTTCCAAATTCCTTTTGATTTTTTTCAAAAAAAAGGGATTAAAG
CTTTGTTTTTTGATTTAGAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKNKIYELTQPGILKLKKELTFLKEVKRKENLEALKEAREQGDLSENADYDAARNEQALIEYRITEIQNILKNVRVVKVS
KDKEINIGKKVSLKMLNSGTEEEFELVSTLESDPFSNKISIESPLGKKIKGHEIGDVVFITSETGTKFKVKILDVK

Sequences:

>Translated_156_residues
MKNKIYELTQPGILKLKKELTFLKEVKRKENLEALKEAREQGDLSENADYDAARNEQALIEYRITEIQNILKNVRVVKVS
KDKEINIGKKVSLKMLNSGTEEEFELVSTLESDPFSNKISIESPLGKKIKGHEIGDVVFITSETGTKFKVKILDVK
>Mature_156_residues
MKNKIYELTQPGILKLKKELTFLKEVKRKENLEALKEAREQGDLSENADYDAARNEQALIEYRITEIQNILKNVRVVKVS
KDKEINIGKKVSLKMLNSGTEEEFELVSTLESDPFSNKISIESPLGKKIKGHEIGDVVFITSETGTKFKVKILDVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=147, Percent_Identity=40.1360544217687, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI87082261, Length=140, Percent_Identity=29.2857142857143, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17767; Mature: 17767

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKIYELTQPGILKLKKELTFLKEVKRKENLEALKEAREQGDLSENADYDAARNEQALI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
EYRITEIQNILKNVRVVKVSKDKEINIGKKVSLKMLNSGTEEEFELVSTLESDPFSNKIS
HHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
IESPLGKKIKGHEIGDVVFITSETGTKFKVKILDVK
ECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MKNKIYELTQPGILKLKKELTFLKEVKRKENLEALKEAREQGDLSENADYDAARNEQALI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
EYRITEIQNILKNVRVVKVSKDKEINIGKKVSLKMLNSGTEEEFELVSTLESDPFSNKIS
HHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEE
IESPLGKKIKGHEIGDVVFITSETGTKFKVKILDVK
ECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA