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The map label for this gene is gyrB [H]

Identifier: 197294338

GI number: 197294338

Start: 250087

End: 252075

Strand: Direct

Name: gyrB [H]

Synonym: PAa_0213

Alternate gene names: 197294338

Gene position: 250087-252075 (Clockwise)

Preceding gene: 197294335

Following gene: 197294339

Centisome position: 28.42

GC content: 31.12

Gene sequence:

>1989_bases
TTGAAAAATTACAACGCAGACAGTATTCAAATTTTAGAGGGTTTGGAAGCAGTTCGTAAAAGACCAGGAATGTATATCGG
CTCTACTGCTGCTAAAGGATTACATCATTTAGTTTGGGAAATTGTTGATAATTCCATTGATGAAGCTTTAGCGGGTCATG
CAAACGAAATCACTTTAGAGATTTTACCCGGAGACATCATTAGTGTAACTGATAACGGTAGAGGAATTCCTGTAGGGATT
CACCACAAAACAGGTAAACCAGCGGTAGAAACCATTTTAACTACTTTGCACGCAGGAGGAAAATTTGACAGCTCTTCTTA
TAAAATTTCGGGCGGTCTTCATGGAGTGGGAGCCTCTGTTGTAAATGCTTTATCTAGTTGGTTTAGTGTTGAAATTCATT
TGGATAAAAAAATCCATTATCAAAAATATGAAAAAGGGGTCCCTGTTGCTCCTTTAGAAGTTATTGGTAAAACAGACCGC
AAAGGGACAGTTATTAAATTTTTAGCTGATCCTTCCATTTTTCAAGAAACTACTATTTACGATGCCAAAATTTTGAAAGA
ACGCATCCAACAATTATCTTTTTTAAATAAAGGACTTAAATTAAATTTAATTGACCAAAGACAAGAAAAACCTGTAAGCT
TTAATTTTTATCACGAAAAAGGCTTGCAAGATTACCTTACTTTTATTAATCAAACTTATATGCAAAAACCTTTTCATAAT
CTTTTTGTTTTAGAAAAAGAACTTGATAATTTGGCTTTAGAAATTGTTTTTGAATACACTGTTAATGATGATGAAAAAAG
TAAACAACAAGAAGAAGAACAAGCAATTAAAAATTACACTCAAACTCAAAAAATTTATTCTTTTGTTAATAACATCCACA
CTCATGAAGGCGGAACACATGAAGAAGGTTTTAAATTGGCTTTAAGTAGGAATTTTTCTAAATATGCTAAAGATTATAAT
CTTTTAAAAAAAAATGAAAGTTTATTAAGTGAAGATATTTTAGAAGGAATCACTGCAATTATTTCTTTAAAGCACCAAGA
TCCACAATTCGAAGGACAAACTAAAGCTAAATTAGGCAATGTTGAAGTAAGACAAATTGTTTCTCAATATTTTGGTGAAG
CTTTAGGAAGATTTTTATTAGAAAATCCAGGTGATGCTAAAAAAATTATCGAGAAATGTTTACTTTCTGCAAACGCTCGT
CTAGCTGCAAAAAGAGCGCGTGAAATTGTAAGAAACAAGCCGCTTGATACTTTAGGTTTTGCTGCTAAATTAGCTGATTG
CCGTAGCAAAGATCCAAAAATTTCTGAACTTTATATTGTCGAAGGAGATTCTGCAGGAGGTTCTGCTAAACAAGGAAGAG
ATTCTCATTTTCAAGCAATTTTGCCTTTAAAAGGGAAAGTGTTAAACGTTGAAAAAACTCAATCTTCCAAAATTTTAACT
AACAAAGAAATCAAATCCTTAATTCAAGCAATTGGCATAGGTGTTGATATCAATAAACAAAAAAACCTCAACTTAGATAA
ATTACGTTATCATAAAATTATTATTATGACCGATGCGGATGTTGATGGTGCTCACATTAGAACCTTGCTTTTAACTTTCT
TTTTCAGAAATTTAAGGATTTTAATTGAAAAAGGTTATATTTATTTCGCAAGACCTCCATTATATAAATATCAAAAAGGT
AAAAATATTACTTATTTTTACGAAGAAAAAGAGAAAATCGATTTCTCCCTCAAAAAAAATATTAAAGATGGTTTTCAAAG
ATATAAAGGATTAGGTGAAATGAATCCGGACCAACTTTGGGAAACTACAATGAATCCTGAAAAACGAACACTTTTACAAG
CCTCTTTAAAAGATGCTTTAAATGCTTTAAGCGACGAAGAAGCGATCAAAGAAGCAAATTCAACTTTTAATATCTTGATG
GGTAAAGAAGTTTTTCCAAGAAAAGAATTCATTTTAAATAATGCTTTAGAAGCGGATTTAGATGTTTAA

Upstream 100 bases:

>100_bases
TATGTTATAATATCATAAGATTGGAATTATTGAAATCGTTTTTTTAGATTCTGATTCTAAAATTAAAAAAATTTCCAAAG
GGAAAAAGGGAAGGTGTTTT

Downstream 100 bases:

>100_bases
AAACTATAAAAACAACCAGCTAATTAAATAAAGTAAAGAGGCAAAAAACATGATTAACCAAAATCCTAATTTGAATCAAG
GAAAGATTAAAGAAGTTAAT

Product: DNA gyrase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MKNYNADSIQILEGLEAVRKRPGMYIGSTAAKGLHHLVWEIVDNSIDEALAGHANEITLEILPGDIISVTDNGRGIPVGI
HHKTGKPAVETILTTLHAGGKFDSSSYKISGGLHGVGASVVNALSSWFSVEIHLDKKIHYQKYEKGVPVAPLEVIGKTDR
KGTVIKFLADPSIFQETTIYDAKILKERIQQLSFLNKGLKLNLIDQRQEKPVSFNFYHEKGLQDYLTFINQTYMQKPFHN
LFVLEKELDNLALEIVFEYTVNDDEKSKQQEEEQAIKNYTQTQKIYSFVNNIHTHEGGTHEEGFKLALSRNFSKYAKDYN
LLKKNESLLSEDILEGITAIISLKHQDPQFEGQTKAKLGNVEVRQIVSQYFGEALGRFLLENPGDAKKIIEKCLLSANAR
LAAKRAREIVRNKPLDTLGFAAKLADCRSKDPKISELYIVEGDSAGGSAKQGRDSHFQAILPLKGKVLNVEKTQSSKILT
NKEIKSLIQAIGIGVDINKQKNLNLDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRNLRILIEKGYIYFARPPLYKYQKG
KNITYFYEEKEKIDFSLKKNIKDGFQRYKGLGEMNPDQLWETTMNPEKRTLLQASLKDALNALSDEEAIKEANSTFNILM
GKEVFPRKEFILNNALEADLDV

Sequences:

>Translated_662_residues
MKNYNADSIQILEGLEAVRKRPGMYIGSTAAKGLHHLVWEIVDNSIDEALAGHANEITLEILPGDIISVTDNGRGIPVGI
HHKTGKPAVETILTTLHAGGKFDSSSYKISGGLHGVGASVVNALSSWFSVEIHLDKKIHYQKYEKGVPVAPLEVIGKTDR
KGTVIKFLADPSIFQETTIYDAKILKERIQQLSFLNKGLKLNLIDQRQEKPVSFNFYHEKGLQDYLTFINQTYMQKPFHN
LFVLEKELDNLALEIVFEYTVNDDEKSKQQEEEQAIKNYTQTQKIYSFVNNIHTHEGGTHEEGFKLALSRNFSKYAKDYN
LLKKNESLLSEDILEGITAIISLKHQDPQFEGQTKAKLGNVEVRQIVSQYFGEALGRFLLENPGDAKKIIEKCLLSANAR
LAAKRAREIVRNKPLDTLGFAAKLADCRSKDPKISELYIVEGDSAGGSAKQGRDSHFQAILPLKGKVLNVEKTQSSKILT
NKEIKSLIQAIGIGVDINKQKNLNLDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRNLRILIEKGYIYFARPPLYKYQKG
KNITYFYEEKEKIDFSLKKNIKDGFQRYKGLGEMNPDQLWETTMNPEKRTLLQASLKDALNALSDEEAIKEANSTFNILM
GKEVFPRKEFILNNALEADLDV
>Mature_662_residues
MKNYNADSIQILEGLEAVRKRPGMYIGSTAAKGLHHLVWEIVDNSIDEALAGHANEITLEILPGDIISVTDNGRGIPVGI
HHKTGKPAVETILTTLHAGGKFDSSSYKISGGLHGVGASVVNALSSWFSVEIHLDKKIHYQKYEKGVPVAPLEVIGKTDR
KGTVIKFLADPSIFQETTIYDAKILKERIQQLSFLNKGLKLNLIDQRQEKPVSFNFYHEKGLQDYLTFINQTYMQKPFHN
LFVLEKELDNLALEIVFEYTVNDDEKSKQQEEEQAIKNYTQTQKIYSFVNNIHTHEGGTHEEGFKLALSRNFSKYAKDYN
LLKKNESLLSEDILEGITAIISLKHQDPQFEGQTKAKLGNVEVRQIVSQYFGEALGRFLLENPGDAKKIIEKCLLSANAR
LAAKRAREIVRNKPLDTLGFAAKLADCRSKDPKISELYIVEGDSAGGSAKQGRDSHFQAILPLKGKVLNVEKTQSSKILT
NKEIKSLIQAIGIGVDINKQKNLNLDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRNLRILIEKGYIYFARPPLYKYQKG
KNITYFYEEKEKIDFSLKKNIKDGFQRYKGLGEMNPDQLWETTMNPEKRTLLQASLKDALNALSDEEAIKEANSTFNILM
GKEVFPRKEFILNNALEADLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=622, Percent_Identity=25.7234726688103, Blast_Score=127, Evalue=5e-29,
Organism=Homo sapiens, GI19913408, Length=625, Percent_Identity=26.4, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI48994957, Length=572, Percent_Identity=48.2517482517483, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1789408, Length=650, Percent_Identity=38.1538461538462, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=25.8652094717668, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17535065, Length=641, Percent_Identity=25.585023400936, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=176, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=332, Percent_Identity=27.1084337349398, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17136538, Length=318, Percent_Identity=26.7295597484277, Blast_Score=76, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74824; Mature: 74824

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00177 TOPOISOMERASE_II ; PS00230 MAP1B_NEURAXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYNADSIQILEGLEAVRKRPGMYIGSTAAKGLHHLVWEIVDNSIDEALAGHANEITLE
CCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ILPGDIISVTDNGRGIPVGIHHKTGKPAVETILTTLHAGGKFDSSSYKISGGLHGVGASV
EECCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
VNALSSWFSVEIHLDKKIHYQKYEKGVPVAPLEVIGKTDRKGTVIKFLADPSIFQETTIY
HHHHHHHEEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
DAKILKERIQQLSFLNKGLKLNLIDQRQEKPVSFNFYHEKGLQDYLTFINQTYMQKPFHN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHCCHHH
LFVLEKELDNLALEIVFEYTVNDDEKSKQQEEEQAIKNYTQTQKIYSFVNNIHTHEGGTH
HEEEHHHHHCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EEGFKLALSRNFSKYAKDYNLLKKNESLLSEDILEGITAIISLKHQDPQFEGQTKAKLGN
CCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
VEVRQIVSQYFGEALGRFLLENPGDAKKIIEKCLLSANARLAAKRAREIVRNKPLDTLGF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
AAKLADCRSKDPKISELYIVEGDSAGGSAKQGRDSHFQAILPLKGKVLNVEKTQSSKILT
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCC
NKEIKSLIQAIGIGVDINKQKNLNLDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRNLRI
HHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHE
LIEKGYIYFARPPLYKYQKGKNITYFYEEKEKIDFSLKKNIKDGFQRYKGLGEMNPDQLW
EEECCEEEEECCCCHHHCCCCCEEEEECCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHH
ETTMNPEKRTLLQASLKDALNALSDEEAIKEANSTFNILMGKEVFPRKEFILNNALEADL
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCC
DV
CC
>Mature Secondary Structure
MKNYNADSIQILEGLEAVRKRPGMYIGSTAAKGLHHLVWEIVDNSIDEALAGHANEITLE
CCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ILPGDIISVTDNGRGIPVGIHHKTGKPAVETILTTLHAGGKFDSSSYKISGGLHGVGASV
EECCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHH
VNALSSWFSVEIHLDKKIHYQKYEKGVPVAPLEVIGKTDRKGTVIKFLADPSIFQETTIY
HHHHHHHEEEEEEECCCHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
DAKILKERIQQLSFLNKGLKLNLIDQRQEKPVSFNFYHEKGLQDYLTFINQTYMQKPFHN
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHCCHHHHHHHHHHHHHHCCHHH
LFVLEKELDNLALEIVFEYTVNDDEKSKQQEEEQAIKNYTQTQKIYSFVNNIHTHEGGTH
HEEEHHHHHCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EEGFKLALSRNFSKYAKDYNLLKKNESLLSEDILEGITAIISLKHQDPQFEGQTKAKLGN
CCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
VEVRQIVSQYFGEALGRFLLENPGDAKKIIEKCLLSANARLAAKRAREIVRNKPLDTLGF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHH
AAKLADCRSKDPKISELYIVEGDSAGGSAKQGRDSHFQAILPLKGKVLNVEKTQSSKILT
HHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCC
NKEIKSLIQAIGIGVDINKQKNLNLDKLRYHKIIIMTDADVDGAHIRTLLLTFFFRNLRI
HHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHE
LIEKGYIYFARPPLYKYQKGKNITYFYEEKEKIDFSLKKNIKDGFQRYKGLGEMNPDQLW
EEECCEEEEECCCCHHHCCCCCEEEEECCCHHCCHHHHHHHHHHHHHHHCCCCCCHHHHH
ETTMNPEKRTLLQASLKDALNALSDEEAIKEANSTFNILMGKEVFPRKEFILNNALEADL
HHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHCCCCCCC
DV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]