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The map label for this gene is prfA

Identifier: 197294284

GI number: 197294284

Start: 175594

End: 176658

Strand: Reverse

Name: prfA

Synonym: PAa_0147

Alternate gene names: 197294284

Gene position: 176658-175594 (Counterclockwise)

Preceding gene: 197294285

Following gene: 197294283

Centisome position: 20.08

GC content: 33.15

Gene sequence:

>1065_bases
ATGCTTGCGAGACTTAAAAAAATTCAACAAAAATATCAAAATTTGCAACAAGATTTGCTTCAAAACCAAAGCATATCTAA
TAAAATTAACATTTTCAAAAATCTCAGCAAATTAGAACCAATAGTAGAACTTTTTCAACAATATTTAACTTTAGAAGAGC
AATTAACAAATATTACAAAAGCCCTTAATCAAGATCAAATCGAAGATAAAGAAATTTTGGAATTGGCTCAAAAAGAAAAA
GAAACGCTTTCACAACAAAAAAAAGTTCTTTGGAATCAATTACGTATTTTATTGTTGCCTCAAGATCCTGAAGACCAAAA
AAACGTGATTTTGGAAATTAAAGGAGCTGTTGGTGGTAATGAAGCCAATCTTTTTGCAGCCGATTTATTAAGAACTTACA
TGAAATATGCTGAAAGTAAAAAATGGAAAGTAGAAATCCTCAATTTACATCCAAGCATTAAAGGTGGCCTGTCTTCGGTA
GAATTATTAATTTCAGGACAAAACATCTACTCTTTTTTAAAATATGAATCAGGAGTTCATCGAGTTCAGCGAGTTCCTGC
AACTGAGGTCCAAGGACGAATTCACACTTCAACCGCAATTGTTTTAGTTCTCCCTGAAGCTAAGGAAGTTGAAATTAAAA
TTGATTGGAATGACATTCGCACAGATACTTTCAACTCAAGTGGACCAGGGGGGCAATCTGTAAACACCACTAAATCAGCT
GTTAGACTTTCTCACCTTCCTTCAGGCATCAGTGTCGCTTGTCAAGAAGGAAAAAGCCAACATGAAAACAAAGAAAAAGC
CTTCACTTTGCTAAAAGCTCGCATTTATAATCAAATATTAAATGCCAAACAAGAAGCAGAAAATAAACATCGCAAAAGTT
TAGTGGGAACAGGTGACCGAAGTGAAAAAATCAGAACTTATAATTATCCTCAAAACCGCATTACAGACCATCGAATTGGA
CTTACTTTGCAAAAATTAGACATCATTATGGAAGGCAAATTAGATTTTATCATTGAACCTTTGATTGATGCCACTCAAAA
AGAAAAATTAGTTCAAAGTGAATAA

Upstream 100 bases:

>100_bases
AAATAGAATCTTATGTTAGCGAAAATTGATTTCAAAATCAAACTTTCCTTCCATTGATTTGGTTTATCGAAAATAAAAAA
AGAGGAGATAAAAGTTAAAT

Downstream 100 bases:

>100_bases
TACAAAACCACTCAAAAACATCATCATTTTTCCCACAGATACAGTTTATGGGATGGGAGTTTCTTTATATGACGAAAAAG
GACTAAAAGAAATTTATCAA

Product: Protein chain release factor A

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLARLKKIQQKYQNLQQDLLQNQSISNKINIFKNLSKLEPIVELFQQYLTLEEQLTNITKALNQDQIEDKEILELAQKEK
ETLSQQKKVLWNQLRILLLPQDPEDQKNVILEIKGAVGGNEANLFAADLLRTYMKYAESKKWKVEILNLHPSIKGGLSSV
ELLISGQNIYSFLKYESGVHRVQRVPATEVQGRIHTSTAIVLVLPEAKEVEIKIDWNDIRTDTFNSSGPGGQSVNTTKSA
VRLSHLPSGISVACQEGKSQHENKEKAFTLLKARIYNQILNAKQEAENKHRKSLVGTGDRSEKIRTYNYPQNRITDHRIG
LTLQKLDIIMEGKLDFIIEPLIDATQKEKLVQSE

Sequences:

>Translated_354_residues
MLARLKKIQQKYQNLQQDLLQNQSISNKINIFKNLSKLEPIVELFQQYLTLEEQLTNITKALNQDQIEDKEILELAQKEK
ETLSQQKKVLWNQLRILLLPQDPEDQKNVILEIKGAVGGNEANLFAADLLRTYMKYAESKKWKVEILNLHPSIKGGLSSV
ELLISGQNIYSFLKYESGVHRVQRVPATEVQGRIHTSTAIVLVLPEAKEVEIKIDWNDIRTDTFNSSGPGGQSVNTTKSA
VRLSHLPSGISVACQEGKSQHENKEKAFTLLKARIYNQILNAKQEAENKHRKSLVGTGDRSEKIRTYNYPQNRITDHRIG
LTLQKLDIIMEGKLDFIIEPLIDATQKEKLVQSE
>Mature_354_residues
MLARLKKIQQKYQNLQQDLLQNQSISNKINIFKNLSKLEPIVELFQQYLTLEEQLTNITKALNQDQIEDKEILELAQKEK
ETLSQQKKVLWNQLRILLLPQDPEDQKNVILEIKGAVGGNEANLFAADLLRTYMKYAESKKWKVEILNLHPSIKGGLSSV
ELLISGQNIYSFLKYESGVHRVQRVPATEVQGRIHTSTAIVLVLPEAKEVEIKIDWNDIRTDTFNSSGPGGQSVNTTKSA
VRLSHLPSGISVACQEGKSQHENKEKAFTLLKARIYNQILNAKQEAENKHRKSLVGTGDRSEKIRTYNYPQNRITDHRIG
LTLQKLDIIMEGKLDFIIEPLIDATQKEKLVQSE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI34577120, Length=360, Percent_Identity=37.7777777777778, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI166795303, Length=312, Percent_Identity=42.9487179487179, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=42.7184466019417, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1787462, Length=356, Percent_Identity=44.9438202247191, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI2367172, Length=317, Percent_Identity=34.0694006309148, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=35.3535353535354, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6321295, Length=361, Percent_Identity=40.9972299168975, Blast_Score=229, Evalue=4e-61,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=38.9570552147239, Blast_Score=218, Evalue=5e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_PHYAS (B1V950)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798825.1
- ProteinModelPortal:   B1V950
- SMR:   B1V950
- GeneID:   6799185
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- ProtClustDB:   CLSK343573
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40359; Mature: 40359

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLARLKKIQQKYQNLQQDLLQNQSISNKINIFKNLSKLEPIVELFQQYLTLEEQLTNITK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNQDQIEDKEILELAQKEKETLSQQKKVLWNQLRILLLPQDPEDQKNVILEIKGAVGGN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCCCC
EANLFAADLLRTYMKYAESKKWKVEILNLHPSIKGGLSSVELLISGQNIYSFLKYESGVH
CHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
RVQRVPATEVQGRIHTSTAIVLVLPEAKEVEIKIDWNDIRTDTFNSSGPGGQSVNTTKSA
HHHHCCCHHHCCEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
VRLSHLPSGISVACQEGKSQHENKEKAFTLLKARIYNQILNAKQEAENKHRKSLVGTGDR
HHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SEKIRTYNYPQNRITDHRIGLTLQKLDIIMEGKLDFIIEPLIDATQKEKLVQSE
CCCCEECCCCCHHCCCHHHCEEHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MLARLKKIQQKYQNLQQDLLQNQSISNKINIFKNLSKLEPIVELFQQYLTLEEQLTNITK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNQDQIEDKEILELAQKEKETLSQQKKVLWNQLRILLLPQDPEDQKNVILEIKGAVGGN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEEECCCCCC
EANLFAADLLRTYMKYAESKKWKVEILNLHPSIKGGLSSVELLISGQNIYSFLKYESGVH
CHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
RVQRVPATEVQGRIHTSTAIVLVLPEAKEVEIKIDWNDIRTDTFNSSGPGGQSVNTTKSA
HHHHCCCHHHCCEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
VRLSHLPSGISVACQEGKSQHENKEKAFTLLKARIYNQILNAKQEAENKHRKSLVGTGDR
HHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SEKIRTYNYPQNRITDHRIGLTLQKLDIIMEGKLDFIIEPLIDATQKEKLVQSE
CCCCEECCCCCHHCCCHHHCEEHHHHHHHHCCCHHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA