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The map label for this gene is potB [H]

Identifier: 197294271

GI number: 197294271

Start: 154501

End: 155337

Strand: Reverse

Name: potB [H]

Synonym: PAa_0134

Alternate gene names: 197294271

Gene position: 155337-154501 (Counterclockwise)

Preceding gene: 197294272

Following gene: 197294270

Centisome position: 17.65

GC content: 28.79

Gene sequence:

>837_bases
ATGAACCAAAAAAAAATTTATGATTGCAAAGATAATCAAAAATTATACCGTTTTTTAATTTTTCCTTATTATGTTCTTTT
GGTAGGATTAATTTTTGTACCCATGGGATTTATTTTTTTTGATTCTTTTCGCGATACTAACAACCCCCATTCTCTTTTTT
CCCAATCTTTAACGTTTGAATATCATAAAGAATTTTTAACTAATCTTTCTTTTTTATATGTTTTATCCAGGTCAATAGCT
ATCTCTATCATTGTTACTTTTTTACTTTTAATCATTGTTTATCCTTTGGCTTATCTTATATCTCGCCTTAATTACATTTT
GCAAACTTTTTTAGTTATTTTAATTAATGGCACAATGTGGATTAATATGATTATCAAAACTCAAGCCTTAGTGCAAATAT
TGCAATTAATCCAAAACAATTTCCAGATTACTTTATTAGAGACCAATTTTGCAATGATACTAGGGTTAATTTATTTATTT
TTGCCTTTTATGTTTTTGCCTGTTTATACTGCAATTGTTAAAATCAATACTCAACACATACAGGCCGCAAAAGACTTAGG
GGCGAGCGACTGGCAAGTATTTAAAAAAATTATTTGGCCTTTGTCGTTGCCTGGAGTTATTACAGGGATTTCTTTGGTGT
TTTTGCAAGTTGCAACAAATATTGTAGCACCACGTTATTTAGGACCTACCACTCAAATAACTATTGCTGAATTAATTGAA
AATAAAACTTTGTTAAGCGGTGAAATTAAGCAAGCTTGTGCGATTGCTATTTACTTATCTTTAGTGATGTTTTTGCTTTT
TTACCTTTTTAAAAAAAGGAGTTACAATGAAATCTAA

Upstream 100 bases:

>100_bases
AAAGAACTTATACCATTCAAACAACAGACCACGTTAAAATGGATAAAGAAGTGGATATTACTTTTAATCCTGAAGATATT
CATGTTATGGAAATTTGGCA

Downstream 100 bases:

>100_bases
TTCTAAAATAAAATTAAAACATATCTTGTATATTGCCATAATTTTAACTTTTATTTATGTTCCAATTGCATCTTTAATTC
TTTTTTCTTTTAATAAAAGC

Product: ABC-type spermidine/putrescine transport system, permease component I

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNQKKIYDCKDNQKLYRFLIFPYYVLLVGLIFVPMGFIFFDSFRDTNNPHSLFSQSLTFEYHKEFLTNLSFLYVLSRSIA
ISIIVTFLLLIIVYPLAYLISRLNYILQTFLVILINGTMWINMIIKTQALVQILQLIQNNFQITLLETNFAMILGLIYLF
LPFMFLPVYTAIVKINTQHIQAAKDLGASDWQVFKKIIWPLSLPGVITGISLVFLQVATNIVAPRYLGPTTQITIAELIE
NKTLLSGEIKQACAIAIYLSLVMFLLFYLFKKRSYNEI

Sequences:

>Translated_278_residues
MNQKKIYDCKDNQKLYRFLIFPYYVLLVGLIFVPMGFIFFDSFRDTNNPHSLFSQSLTFEYHKEFLTNLSFLYVLSRSIA
ISIIVTFLLLIIVYPLAYLISRLNYILQTFLVILINGTMWINMIIKTQALVQILQLIQNNFQITLLETNFAMILGLIYLF
LPFMFLPVYTAIVKINTQHIQAAKDLGASDWQVFKKIIWPLSLPGVITGISLVFLQVATNIVAPRYLGPTTQITIAELIE
NKTLLSGEIKQACAIAIYLSLVMFLLFYLFKKRSYNEI
>Mature_278_residues
MNQKKIYDCKDNQKLYRFLIFPYYVLLVGLIFVPMGFIFFDSFRDTNNPHSLFSQSLTFEYHKEFLTNLSFLYVLSRSIA
ISIIVTFLLLIIVYPLAYLISRLNYILQTFLVILINGTMWINMIIKTQALVQILQLIQNNFQITLLETNFAMILGLIYLF
LPFMFLPVYTAIVKINTQHIQAAKDLGASDWQVFKKIIWPLSLPGVITGISLVFLQVATNIVAPRYLGPTTQITIAELIE
NKTLLSGEIKQACAIAIYLSLVMFLLFYLFKKRSYNEI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=215, Percent_Identity=31.1627906976744, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787369, Length=259, Percent_Identity=30.1158301158301, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787713, Length=261, Percent_Identity=23.7547892720307, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32162; Mature: 32162

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]