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The map label for this gene is leuS [H]

Identifier: 197294242

GI number: 197294242

Start: 112526

End: 114970

Strand: Direct

Name: leuS [H]

Synonym: PAa_0104

Alternate gene names: 197294242

Gene position: 112526-114970 (Clockwise)

Preceding gene: 197294241

Following gene: 197294243

Centisome position: 12.79

GC content: 29.94

Gene sequence:

>2445_bases
ATGGCTAAATTAAATTATGATTTTAAAAAAATAGAAGAACATTGGCAAGATTATTGGAAAAAACACGAGATTTTTAAAAC
AAAAATTAATAAAGATCAAAAGAAATTTTATTGTTTAGATATGTTTCCTTATCCTTCATCAGAAGGATTACATGTTGGTC
ATATTGAAGGTTATGCCGCAACCGATATTGTAAGCCGTTTTAAAAGAATGCAACAATACAACGTTTTTCATCCTTTTGGT
TGGGATTCTTTTGGACTTCCAGCAGAACAATACGCTTTGAAAACAGGAAAAAATCCTAGAAATTTTACTTATGAAAATAT
TCAGAAATTTAAAATACAAATTCAAAGACTCGGAAAAAGTGTTGATTGGGAAAAAGAATTAGCAACTTCAGATCCTTATT
TTTATAAATGGACTCAATGGATTTTTAAAAAACTTTACGAAAAAAATTTAGCCGTTTTGCAAAATGTAGAAGTTTATTTT
TGTCCTGCATTAGGAACAGTTCTTGCCAATGAAGAAGTTATTTCTAACGATAAAGGTCTTTTTTCTGAAAGAGGGAATCA
TCCTGTTATTAAAAAACAAATGAAACAATGGGTTTTAAAAATTACTGATTATGCTGAAAGACTTTTAGCTGATTTAGATC
TTGTTGATTGGCCGCAAAACGTTAAAGATATGCAAATTAATTGGATTGGTAAATCTCAAGGAGCTGTTGTTTCTTTTCCT
GTATTTCAACAAAAAAATACTTTAAAAGTCTTTACTACTCGCCCTTATACTCTTTTTGGTGTTACTTTTTTAGTTATAGC
TCCTGAACATGAATTATTATCACAAATTACTACTTTAAAACAGCAAGAAGAAGTTTTGGATTATCTAGAATGCACTAAAC
AAAAAAAAACCTTAGAAAGAAACATTAACAAAGATAAAAGTGGTGTTTTTACTGGAAGTTATGCTATTAATCCGTGTAAT
GGTGAAAAAATTCCTATTTGGGTGGATGATTACGTTTTGCCTCATTTTGGCACAGGCATTTTAATGGGCGTTCCTTGTCA
TGATCAAAGAGATTTTGAATTTGCACAAAAACACCATTTAAAAATGATTCAAGTAGTAAAACCAAACCCAATCACTGATA
GTAGCCAAAAAAACCCTTTAGAAGTTTTTGAAGGAGATGGCATTATTGTTAATAGTGATTTTTTAAATGGTTTAGACAAC
AATCAAGCACAAACAAAGATGATGCAATTTTTAAAAGAAAAAAAATTAGCCTATCCTCATTATACTTATAAATTGCATGA
TTGGGTTTTTTCTCGCCAAAGATATTGGGGAGAACCATTATCTATTTTTTATGATGAAAATAATAATATTCACACTATGG
ATGATGACTCTTTGCCTTTGGAATTGCCTGTTTTAGATAAAATTCAACCTTCAGGAACGGGAGAATCGCCATTATCAAAA
GCATCTTCTTGGTTGTATTTTGAAAAAGATGGCAAAAAGTACCGAAGAGATTCCAATACTATGCCACAACTTGCAGGAAG
CTCTTGGTATTATATTGCTTATGTTTTAAAAAACATTTCAGGGATCACCCCTTTAAACACATCCGAAGCGAAAGAGTTAT
TAGACTATTACTTGCCTGTTGATTTGTATATTGGCGGGACAGAACATGCAGTCGGTCATTTATTATATGCTCGTTTTTGG
CATAAATTTTTATATGATTTGGGTTTAGTTTCTCAGAAAGAACCTTTTCAAAAATTAGTTAATCAAGGAATGATTTTAGG
ACATGACCATACTAAAATGTCTAAATCAAAAGGCAATGGCGTAAATGCTTCTTCAATGTTAGAAAAGTATGGCGCGGACG
CCTTAAGGTTGCATATTATGTTTATGGGTCCTTTAGAAGATGTTAAAAGTTGGAACGAAAAAGGTTTGCAAGGGATACAA
CGTTTTTTAAATAGAGTTCATCAGATGTGTTCTTTTGAAATGATAGATCAAAAAGATGTTTTTTTAGAAAAAATTTTGCA
TCAAACAATTCAAATAGTTACTCAAAGTTATGAAAAATTAAAATTTAACAAAGCTATTAGTCAACTAATGATTTTTGTTA
ATCACGTTTACAAATCCCAAAAAATCACTAAAGAGCAATGCCAAATTTTTTTACAAATGTTAAACCCCCTCACACCTCAT
TTGACAGAAGAGTTAAATGCAACAATTTTGAAGAATAAGGAAATGTTAGTTAATATGACTTGGCCTCATTATAATCCTAA
ATATTTAGGAAACGAACAATCAAATATTATTGTTCAAGTTAATGGCAAAACACGCTCTATTTTAACTGTATCTTTGGGCG
TTTCTTCAGAGAAAATCCAAAAATTAGCTTTTCAAGATCAAAAAGTACATAAATTTATTTTTCAAAAACAAATTCAGAAA
GTAATTTATATTCCTGAAAAATTATTAAATATCATTGTTATTTGA

Upstream 100 bases:

>100_bases
TTTAATATTTTACTTTTTTCATTCCCGTTTTGCGGCAGTATCCAGGCCAATAATTAATAACATATCTTTTGGCTTCAAAA
ATAATAGAAAGTTTGTAATA

Downstream 100 bases:

>100_bases
TTATTTAATTTTGCTTTTAAATTCTTAATAAAATTCAAGAAGTGTTTTTTCAAATTCTATTCAAATAAGTTTATGTAAAT
TTTGGAGAAAAAGGTATGTT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MAKLNYDFKKIEEHWQDYWKKHEIFKTKINKDQKKFYCLDMFPYPSSEGLHVGHIEGYAATDIVSRFKRMQQYNVFHPFG
WDSFGLPAEQYALKTGKNPRNFTYENIQKFKIQIQRLGKSVDWEKELATSDPYFYKWTQWIFKKLYEKNLAVLQNVEVYF
CPALGTVLANEEVISNDKGLFSERGNHPVIKKQMKQWVLKITDYAERLLADLDLVDWPQNVKDMQINWIGKSQGAVVSFP
VFQQKNTLKVFTTRPYTLFGVTFLVIAPEHELLSQITTLKQQEEVLDYLECTKQKKTLERNINKDKSGVFTGSYAINPCN
GEKIPIWVDDYVLPHFGTGILMGVPCHDQRDFEFAQKHHLKMIQVVKPNPITDSSQKNPLEVFEGDGIIVNSDFLNGLDN
NQAQTKMMQFLKEKKLAYPHYTYKLHDWVFSRQRYWGEPLSIFYDENNNIHTMDDDSLPLELPVLDKIQPSGTGESPLSK
ASSWLYFEKDGKKYRRDSNTMPQLAGSSWYYIAYVLKNISGITPLNTSEAKELLDYYLPVDLYIGGTEHAVGHLLYARFW
HKFLYDLGLVSQKEPFQKLVNQGMILGHDHTKMSKSKGNGVNASSMLEKYGADALRLHIMFMGPLEDVKSWNEKGLQGIQ
RFLNRVHQMCSFEMIDQKDVFLEKILHQTIQIVTQSYEKLKFNKAISQLMIFVNHVYKSQKITKEQCQIFLQMLNPLTPH
LTEELNATILKNKEMLVNMTWPHYNPKYLGNEQSNIIVQVNGKTRSILTVSLGVSSEKIQKLAFQDQKVHKFIFQKQIQK
VIYIPEKLLNIIVI

Sequences:

>Translated_814_residues
MAKLNYDFKKIEEHWQDYWKKHEIFKTKINKDQKKFYCLDMFPYPSSEGLHVGHIEGYAATDIVSRFKRMQQYNVFHPFG
WDSFGLPAEQYALKTGKNPRNFTYENIQKFKIQIQRLGKSVDWEKELATSDPYFYKWTQWIFKKLYEKNLAVLQNVEVYF
CPALGTVLANEEVISNDKGLFSERGNHPVIKKQMKQWVLKITDYAERLLADLDLVDWPQNVKDMQINWIGKSQGAVVSFP
VFQQKNTLKVFTTRPYTLFGVTFLVIAPEHELLSQITTLKQQEEVLDYLECTKQKKTLERNINKDKSGVFTGSYAINPCN
GEKIPIWVDDYVLPHFGTGILMGVPCHDQRDFEFAQKHHLKMIQVVKPNPITDSSQKNPLEVFEGDGIIVNSDFLNGLDN
NQAQTKMMQFLKEKKLAYPHYTYKLHDWVFSRQRYWGEPLSIFYDENNNIHTMDDDSLPLELPVLDKIQPSGTGESPLSK
ASSWLYFEKDGKKYRRDSNTMPQLAGSSWYYIAYVLKNISGITPLNTSEAKELLDYYLPVDLYIGGTEHAVGHLLYARFW
HKFLYDLGLVSQKEPFQKLVNQGMILGHDHTKMSKSKGNGVNASSMLEKYGADALRLHIMFMGPLEDVKSWNEKGLQGIQ
RFLNRVHQMCSFEMIDQKDVFLEKILHQTIQIVTQSYEKLKFNKAISQLMIFVNHVYKSQKITKEQCQIFLQMLNPLTPH
LTEELNATILKNKEMLVNMTWPHYNPKYLGNEQSNIIVQVNGKTRSILTVSLGVSSEKIQKLAFQDQKVHKFIFQKQIQK
VIYIPEKLLNIIVI
>Mature_813_residues
AKLNYDFKKIEEHWQDYWKKHEIFKTKINKDQKKFYCLDMFPYPSSEGLHVGHIEGYAATDIVSRFKRMQQYNVFHPFGW
DSFGLPAEQYALKTGKNPRNFTYENIQKFKIQIQRLGKSVDWEKELATSDPYFYKWTQWIFKKLYEKNLAVLQNVEVYFC
PALGTVLANEEVISNDKGLFSERGNHPVIKKQMKQWVLKITDYAERLLADLDLVDWPQNVKDMQINWIGKSQGAVVSFPV
FQQKNTLKVFTTRPYTLFGVTFLVIAPEHELLSQITTLKQQEEVLDYLECTKQKKTLERNINKDKSGVFTGSYAINPCNG
EKIPIWVDDYVLPHFGTGILMGVPCHDQRDFEFAQKHHLKMIQVVKPNPITDSSQKNPLEVFEGDGIIVNSDFLNGLDNN
QAQTKMMQFLKEKKLAYPHYTYKLHDWVFSRQRYWGEPLSIFYDENNNIHTMDDDSLPLELPVLDKIQPSGTGESPLSKA
SSWLYFEKDGKKYRRDSNTMPQLAGSSWYYIAYVLKNISGITPLNTSEAKELLDYYLPVDLYIGGTEHAVGHLLYARFWH
KFLYDLGLVSQKEPFQKLVNQGMILGHDHTKMSKSKGNGVNASSMLEKYGADALRLHIMFMGPLEDVKSWNEKGLQGIQR
FLNRVHQMCSFEMIDQKDVFLEKILHQTIQIVTQSYEKLKFNKAISQLMIFVNHVYKSQKITKEQCQIFLQMLNPLTPHL
TEELNATILKNKEMLVNMTWPHYNPKYLGNEQSNIIVQVNGKTRSILTVSLGVSSEKIQKLAFQDQKVHKFIFQKQIQKV
IYIPEKLLNIIVI

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=869, Percent_Identity=30.1495972382048, Blast_Score=358, Evalue=1e-98,
Organism=Homo sapiens, GI94721239, Length=716, Percent_Identity=23.6033519553073, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI94721241, Length=716, Percent_Identity=23.6033519553073, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1786861, Length=878, Percent_Identity=35.9908883826879, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1790708, Length=401, Percent_Identity=24.4389027431421, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71997510, Length=282, Percent_Identity=36.8794326241135, Blast_Score=178, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71997517, Length=274, Percent_Identity=37.5912408759124, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI212645227, Length=304, Percent_Identity=25, Blast_Score=103, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71980946, Length=160, Percent_Identity=32.5, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323414, Length=825, Percent_Identity=33.0909090909091, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321531, Length=917, Percent_Identity=19.5201744820065, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319395, Length=760, Percent_Identity=22.7631578947368, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21355409, Length=900, Percent_Identity=28.6666666666667, Blast_Score=300, Evalue=3e-81,
Organism=Drosophila melanogaster, GI28574730, Length=195, Percent_Identity=30.2564102564103, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668547, Length=195, Percent_Identity=30.2564102564103, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668543, Length=195, Percent_Identity=30.2564102564103, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 94838; Mature: 94707

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLNYDFKKIEEHWQDYWKKHEIFKTKINKDQKKFYCLDMFPYPSSEGLHVGHIEGYAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCHHH
TDIVSRFKRMQQYNVFHPFGWDSFGLPAEQYALKTGKNPRNFTYENIQKFKIQIQRLGKS
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
VDWEKELATSDPYFYKWTQWIFKKLYEKNLAVLQNVEVYFCPALGTVLANEEVISNDKGL
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEECCHHHHHHCCCHHHCCCCCC
FSERGNHPVIKKQMKQWVLKITDYAERLLADLDLVDWPQNVKDMQINWIGKSQGAVVSFP
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEC
VFQQKNTLKVFTTRPYTLFGVTFLVIAPEHELLSQITTLKQQEEVLDYLECTKQKKTLER
CCCCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NINKDKSGVFTGSYAINPCNGEKIPIWVDDYVLPHFGTGILMGVPCHDQRDFEFAQKHHL
CCCCCCCCEEECCEEECCCCCCEEEEEECCEECCCCCCCEEEECCCCCCCCHHHHHHHCC
KMIQVVKPNPITDSSQKNPLEVFEGDGIIVNSDFLNGLDNNQAQTKMMQFLKEKKLAYPH
EEEEEECCCCCCCCCCCCCCEEECCCCEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCC
YTYKLHDWVFSRQRYWGEPLSIFYDENNNIHTMDDDSLPLELPVLDKIQPSGTGESPLSK
EEEEHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCEECCCHHCCCCCCCCCCCHHH
ASSWLYFEKDGKKYRRDSNTMPQLAGSSWYYIAYVLKNISGITPLNTSEAKELLDYYLPV
CCCEEEECCCCHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCE
DLYIGGTEHAVGHLLYARFWHKFLYDLGLVSQKEPFQKLVNQGMILGHDHTKMSKSKGNG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECCCCHHCCCCCCC
VNASSMLEKYGADALRLHIMFMGPLEDVKSWNEKGLQGIQRFLNRVHQMCSFEMIDQKDV
CCHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FLEKILHQTIQIVTQSYEKLKFNKAISQLMIFVNHVYKSQKITKEQCQIFLQMLNPLTPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
LTEELNATILKNKEMLVNMTWPHYNPKYLGNEQSNIIVQVNGKTRSILTVSLGVSSEKIQ
HHHHHCHHEEECCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHH
KLAFQDQKVHKFIFQKQIQKVIYIPEKLLNIIVI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
AKLNYDFKKIEEHWQDYWKKHEIFKTKINKDQKKFYCLDMFPYPSSEGLHVGHIEGYAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEECCCCHHH
TDIVSRFKRMQQYNVFHPFGWDSFGLPAEQYALKTGKNPRNFTYENIQKFKIQIQRLGKS
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCC
VDWEKELATSDPYFYKWTQWIFKKLYEKNLAVLQNVEVYFCPALGTVLANEEVISNDKGL
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCEEECCHHHHHHCCCHHHCCCCCC
FSERGNHPVIKKQMKQWVLKITDYAERLLADLDLVDWPQNVKDMQINWIGKSQGAVVSFP
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEC
VFQQKNTLKVFTTRPYTLFGVTFLVIAPEHELLSQITTLKQQEEVLDYLECTKQKKTLER
CCCCCCCEEEEECCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NINKDKSGVFTGSYAINPCNGEKIPIWVDDYVLPHFGTGILMGVPCHDQRDFEFAQKHHL
CCCCCCCCEEECCEEECCCCCCEEEEEECCEECCCCCCCEEEECCCCCCCCHHHHHHHCC
KMIQVVKPNPITDSSQKNPLEVFEGDGIIVNSDFLNGLDNNQAQTKMMQFLKEKKLAYPH
EEEEEECCCCCCCCCCCCCCEEECCCCEEEECHHHCCCCCCHHHHHHHHHHHHHCCCCCC
YTYKLHDWVFSRQRYWGEPLSIFYDENNNIHTMDDDSLPLELPVLDKIQPSGTGESPLSK
EEEEHHHHHHHHHHHCCCCEEEEEECCCCEEECCCCCCCEECCCHHCCCCCCCCCCCHHH
ASSWLYFEKDGKKYRRDSNTMPQLAGSSWYYIAYVLKNISGITPLNTSEAKELLDYYLPV
CCCEEEECCCCHHHHCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCE
DLYIGGTEHAVGHLLYARFWHKFLYDLGLVSQKEPFQKLVNQGMILGHDHTKMSKSKGNG
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECCCCHHCCCCCCC
VNASSMLEKYGADALRLHIMFMGPLEDVKSWNEKGLQGIQRFLNRVHQMCSFEMIDQKDV
CCHHHHHHHHCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FLEKILHQTIQIVTQSYEKLKFNKAISQLMIFVNHVYKSQKITKEQCQIFLQMLNPLTPH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHH
LTEELNATILKNKEMLVNMTWPHYNPKYLGNEQSNIIVQVNGKTRSILTVSLGVSSEKIQ
HHHHHCHHEEECCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCHHHHH
KLAFQDQKVHKFIFQKQIQKVIYIPEKLLNIIVI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA