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The map label for this gene is folA [H]

Identifier: 197294227

GI number: 197294227

Start: 93356

End: 93853

Strand: Direct

Name: folA [H]

Synonym: PAa_0085

Alternate gene names: 197294227

Gene position: 93356-93853 (Clockwise)

Preceding gene: 197294226

Following gene: 197294228

Centisome position: 10.61

GC content: 26.1

Gene sequence:

>498_bases
ATGATTAATTTAATCACAGCTTTTGATAGTAATTATCTAATAGGAAGCGATAATAAACTTCCTTGGCATTATCCTCAAGA
TTTGCATTTTTTTAAAAAAACAACTTTGAATCAAGAAGTTTTAATGGGTCATCAAACTTATCTTTCGTTAAAAAGTTATT
TCAAAGACAAGCCTTTTAATTTTAAAAAAACTTATGTTGCCGCTCGAAATAAAGGTTTGCAATTGGATGGTTGTGAAGTG
GTTTTTGATTTGTTAACTTTTTTAAAAACTTTTTTAATTCAGAAAAAATCGCATCTTTTTGTTATTGGAGGTAGACAAAT
TTATCAACAAAGTTTGCCGTTTGTGGATAATTTATATATTACTTATATTTTAAATAGACATAAAGGGAATGTTTATTTTC
CTCCAGTCAATTGGCAAGATTTTCAATTAATTCAAAAAACGATTCAACCTCAATTAATTTTTGCTTTTTATAAAAGGAAA
AAGGATCCATTATGTTAA

Upstream 100 bases:

>100_bases
TTTTAAACCCTCATATTAAAGATATTGATGGCTTTTCATTTGAAGATTTTACTTTAAAAAATTATTTTTGCGATGGAACT
TTGAAAGGAGATATTGCTAT

Downstream 100 bases:

>100_bases
TTGTCTTTTTTTTCACTATGATTGTTTCTAATTTTTTTTATTTGAAACATTTTTATTTGAATAACCCTATTATAATTTTT
CTTTTATCCTTAATAATGGC

Product: Dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MINLITAFDSNYLIGSDNKLPWHYPQDLHFFKKTTLNQEVLMGHQTYLSLKSYFKDKPFNFKKTYVAARNKGLQLDGCEV
VFDLLTFLKTFLIQKKSHLFVIGGRQIYQQSLPFVDNLYITYILNRHKGNVYFPPVNWQDFQLIQKTIQPQLIFAFYKRK
KDPLC

Sequences:

>Translated_165_residues
MINLITAFDSNYLIGSDNKLPWHYPQDLHFFKKTTLNQEVLMGHQTYLSLKSYFKDKPFNFKKTYVAARNKGLQLDGCEV
VFDLLTFLKTFLIQKKSHLFVIGGRQIYQQSLPFVDNLYITYILNRHKGNVYFPPVNWQDFQLIQKTIQPQLIFAFYKRK
KDPLC
>Mature_165_residues
MINLITAFDSNYLIGSDNKLPWHYPQDLHFFKKTTLNQEVLMGHQTYLSLKSYFKDKPFNFKKTYVAARNKGLQLDGCEV
VFDLLTFLKTFLIQKKSHLFVIGGRQIYQQSLPFVDNLYITYILNRHKGNVYFPPVNWQDFQLIQKTIQPQLIFAFYKRK
KDPLC

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=147, Percent_Identity=31.2925170068027, Blast_Score=84, Evalue=6e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19596; Mature: 19596

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLITAFDSNYLIGSDNKLPWHYPQDLHFFKKTTLNQEVLMGHQTYLSLKSYFKDKPFN
CCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
FKKTYVAARNKGLQLDGCEVVFDLLTFLKTFLIQKKSHLFVIGGRQIYQQSLPFVDNLYI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCHHCEEE
TYILNRHKGNVYFPPVNWQDFQLIQKTIQPQLIFAFYKRKKDPLC
EEEEECCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINLITAFDSNYLIGSDNKLPWHYPQDLHFFKKTTLNQEVLMGHQTYLSLKSYFKDKPFN
CCEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
FKKTYVAARNKGLQLDGCEVVFDLLTFLKTFLIQKKSHLFVIGGRQIYQQSLPFVDNLYI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHCCCCHHCEEE
TYILNRHKGNVYFPPVNWQDFQLIQKTIQPQLIFAFYKRKKDPLC
EEEEECCCCCEEECCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]