Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 197294195

GI number: 197294195

Start: 41240

End: 42523

Strand: Direct

Name: dnaB [H]

Synonym: PAa_0037

Alternate gene names: 197294195

Gene position: 41240-42523 (Clockwise)

Preceding gene: 197294183

Following gene: 197294196

Centisome position: 4.69

GC content: 29.52

Gene sequence:

>1284_bases
ATGATTGAAGCTGAACGTGCATTATTAGGAACACTATTGTTAAATCCAGAAAAAATGGATGCTGTTATAAATTTAGTTAA
CACAAACAATTTTTCCAACCCCAAGCATCGTTGTATCTTTGAGACAATGAAGCGATTAAAACTGCAAAACCGCGAAATTG
ATTATGTTACGGTGGGTTCTGTTTTAGATAACAATTTATACAAAATAGGAGGAATTGATTATTTAATTGAATTAGTGGAG
ATGGCTCCTGCAAGTGAATATTTAGAGACATATATTGATTTAATTAAAGAAAATGCCCTTAAAAGAGATTTGTTAGGATT
AATTAAGAAATTACCTACTGAATTATCTAAATCAAAAAATATCCATAATTATTTGCAAACAGTCAAAAACCAAGTAGAAG
GTTTTATGCAAAAAACCAAATCTCCTTTTATTTCTACAAAAACATTAATTCCCTATATTCGACAAAGTATAGTTAATGAT
GAAAAAGACAACCAACTTGTAGGAATAAAAACAGGCTTTAAAAATCTAGATGAATTAATTTCTGGTTTCAAACCAAACCA
ATTAATTATCTTAGGTGCAAGAACTGGAATGGGGAAAACTGCTTTTATGTTAAATTTGGCTTGTAATATTGCAAAAAACT
TTACTACCAACCCCGAAAACCAAAAAGCAGTTATTTTTAGTTTAGAAATGGCAGCGGAAGAATTAGGAATTAGGTTACTT
TCTTCTGCTTCTCAAATCCCTTTAAAACAACTCCAACACAAAAACTTAACTAAAAATGACAAATTACAGTTACTTATCGC
TGATGACGAAATCACGCAATTAAACATTTTAATTGATGATGATAGAAATAACAAAATTGAAGATATTAAAACCAAATGCC
GTCAAATGAAATATACTAAGGGACTTGATATTGTCTTCATTGATTACCTCCATTTATTAAAAGACGACCAAAATTTTAAT
ACATATCAAGCAATATTTGTGATTTCACGCAAATTAAAAAAACTAGCAAGTGAACTTAATATTCCAATTGTGGCTTTAAG
TCAAATGAACCGTGCAACTTATGTAAGAGAAGTTAAAAGCCCACAATTAACAGATTTAAGAGATTCAGGAACAATCGAAC
AAGACGCTGATGTTGTGATGTTATTACATTGTGAAAGTTATTATCAAAAACCTGATATTAACCCACATACGAATTTAATT
ATTGCCAAAAACCGTAGCGGTCAATTAGGCGAATGTAGTTTTAATTTTTATAAACAAATCCAAAGATTTGAAGAAAAAAA
ATAA

Upstream 100 bases:

>100_bases
GTACTGGTTGAGAACAACAAATTTATCCAAATTGTTTATTAATTAAAAATAAACAGTTTGGATTTTTTTGTATCCAAACT
GAGAAAAAGGAGTTTGAAAG

Downstream 100 bases:

>100_bases
GAATAAAAAAGGAAATAAAAATATGAAACAAATATCTAATTTAGATTTTAATAATATTAACGTTTTTAATCATAAATCTT
TGTGCGTTAACATAACCAAA

Product: Replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MIEAERALLGTLLLNPEKMDAVINLVNTNNFSNPKHRCIFETMKRLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
MAPASEYLETYIDLIKENALKRDLLGLIKKLPTELSKSKNIHNYLQTVKNQVEGFMQKTKSPFISTKTLIPYIRQSIVND
EKDNQLVGIKTGFKNLDELISGFKPNQLIILGARTGMGKTAFMLNLACNIAKNFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDEITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQNFN
TYQAIFVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHCESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEEKK

Sequences:

>Translated_427_residues
MIEAERALLGTLLLNPEKMDAVINLVNTNNFSNPKHRCIFETMKRLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
MAPASEYLETYIDLIKENALKRDLLGLIKKLPTELSKSKNIHNYLQTVKNQVEGFMQKTKSPFISTKTLIPYIRQSIVND
EKDNQLVGIKTGFKNLDELISGFKPNQLIILGARTGMGKTAFMLNLACNIAKNFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDEITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQNFN
TYQAIFVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHCESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEEKK
>Mature_427_residues
MIEAERALLGTLLLNPEKMDAVINLVNTNNFSNPKHRCIFETMKRLKLQNREIDYVTVGSVLDNNLYKIGGIDYLIELVE
MAPASEYLETYIDLIKENALKRDLLGLIKKLPTELSKSKNIHNYLQTVKNQVEGFMQKTKSPFISTKTLIPYIRQSIVND
EKDNQLVGIKTGFKNLDELISGFKPNQLIILGARTGMGKTAFMLNLACNIAKNFTTNPENQKAVIFSLEMAAEELGIRLL
SSASQIPLKQLQHKNLTKNDKLQLLIADDEITQLNILIDDDRNNKIEDIKTKCRQMKYTKGLDIVFIDYLHLLKDDQNFN
TYQAIFVISRKLKKLASELNIPIVALSQMNRATYVREVKSPQLTDLRDSGTIEQDADVVMLLHCESYYQKPDINPHTNLI
IAKNRSGQLGECSFNFYKQIQRFEEKK

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=30.7339449541284, Blast_Score=209, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 48865; Mature: 48865

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAERALLGTLLLNPEKMDAVINLVNTNNFSNPKHRCIFETMKRLKLQNREIDYVTVGS
CCCHHHHHHHHHHCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECH
VLDNNLYKIGGIDYLIELVEMAPASEYLETYIDLIKENALKRDLLGLIKKLPTELSKSKN
HHCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHNYLQTVKNQVEGFMQKTKSPFISTKTLIPYIRQSIVNDEKDNQLVGIKTGFKNLDELI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
SGFKPNQLIILGARTGMGKTAFMLNLACNIAKNFTTNPENQKAVIFSLEMAAEELGIRLL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHH
SSASQIPLKQLQHKNLTKNDKLQLLIADDEITQLNILIDDDRNNKIEDIKTKCRQMKYTK
HCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GLDIVFIDYLHLLKDDQNFNTYQAIFVISRKLKKLASELNIPIVALSQMNRATYVREVKS
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHCC
PQLTDLRDSGTIEQDADVVMLLHCESYYQKPDINPHTNLIIAKNRSGQLGECSFNFYKQI
CCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
QRFEEKK
HHHHCCC
>Mature Secondary Structure
MIEAERALLGTLLLNPEKMDAVINLVNTNNFSNPKHRCIFETMKRLKLQNREIDYVTVGS
CCCHHHHHHHHHHCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECH
VLDNNLYKIGGIDYLIELVEMAPASEYLETYIDLIKENALKRDLLGLIKKLPTELSKSKN
HHCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IHNYLQTVKNQVEGFMQKTKSPFISTKTLIPYIRQSIVNDEKDNQLVGIKTGFKNLDELI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
SGFKPNQLIILGARTGMGKTAFMLNLACNIAKNFTTNPENQKAVIFSLEMAAEELGIRLL
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHH
SSASQIPLKQLQHKNLTKNDKLQLLIADDEITQLNILIDDDRNNKIEDIKTKCRQMKYTK
HCCCCCCHHHHHHCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
GLDIVFIDYLHLLKDDQNFNTYQAIFVISRKLKKLASELNIPIVALSQMNRATYVREVKS
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHHHHCC
PQLTDLRDSGTIEQDADVVMLLHCESYYQKPDINPHTNLIIAKNRSGQLGECSFNFYKQI
CCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHH
QRFEEKK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]