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The map label for this gene is pfpI [H]

Identifier: 197120410

GI number: 197120410

Start: 4602548

End: 4603057

Strand: Direct

Name: pfpI [H]

Synonym: Gbem_4051

Alternate gene names: 197120410

Gene position: 4602548-4603057 (Clockwise)

Preceding gene: 197120409

Following gene: NA

Centisome position: 99.73

GC content: 60.98

Gene sequence:

>510_bases
ATGAAAGCCCTGATTTTAAGTGCGGATAATTTCGAAGATACCGAATTGCTGGTTCCTCTTTACAGACTGCGCGAGGCCGG
GTATGCGGTCGACGTGGCATCGGAAAAGGCGGGTTCCATACACGGTAAGCACGGTTACGAGGTGCCGGTGAACAAGCTGT
TCAACGAGGTGACGCCGGCCGATTACGCGGTCCTGGTACTCCCTGGCGGCAAAGCGCCCGCGGCCATCCGCAACAGCCCG
GCGGTCCATGAAATCGCCCGCGCCTTCATGTCGGCGTCCAAACCAGTGGCCGCCATCTGCCACGGCCCGCAGATCCTGAT
CTCCGCCGGACTGCTCAAGGGGCGCAAGGCGACCTGCTATGAGTCGGTGGCCCCTGAACTGCGGGACGCCCAGGCGCAGT
ACCAGGATGTCGAGGTGATAGTGGACGGAAACCTGATCACCTCAAGAAAACCCGACGATCTGCCCGCCTTCTGCCGCGAG
TTGGTGCGTATGGTCAAGGCGGCTGCGTAA

Upstream 100 bases:

>100_bases
ATTGAGTTACACTAAGAAAAGGCATGGTAAACTGATCTCCGTCGCTCACCACAGACCTGCCGCCAGCGGTAGCTTAACTA
AACGGAGCAGAGGAGGAGTC

Downstream 100 bases:

>100_bases
ACCGAAGAAAAGGCATGCACCACAGAGGACACGGAGGTACACAGAGGAAAACCAGAACCGGGTACAACCAAAAGGTTTAT
CTTAAAGCCTTGGCTTTTCC

Product: intracellular protease, PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKALILSADNFEDTELLVPLYRLREAGYAVDVASEKAGSIHGKHGYEVPVNKLFNEVTPADYAVLVLPGGKAPAAIRNSP
AVHEIARAFMSASKPVAAICHGPQILISAGLLKGRKATCYESVAPELRDAQAQYQDVEVIVDGNLITSRKPDDLPAFCRE
LVRMVKAAA

Sequences:

>Translated_169_residues
MKALILSADNFEDTELLVPLYRLREAGYAVDVASEKAGSIHGKHGYEVPVNKLFNEVTPADYAVLVLPGGKAPAAIRNSP
AVHEIARAFMSASKPVAAICHGPQILISAGLLKGRKATCYESVAPELRDAQAQYQDVEVIVDGNLITSRKPDDLPAFCRE
LVRMVKAAA
>Mature_169_residues
MKALILSADNFEDTELLVPLYRLREAGYAVDVASEKAGSIHGKHGYEVPVNKLFNEVTPADYAVLVLPGGKAPAAIRNSP
AVHEIARAFMSASKPVAAICHGPQILISAGLLKGRKATCYESVAPELRDAQAQYQDVEVIVDGNLITSRKPDDLPAFCRE
LVRMVKAAA

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=169, Percent_Identity=43.1952662721894, Blast_Score=142, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653499, Length=160, Percent_Identity=35.625, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571932, Length=151, Percent_Identity=32.4503311258278, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 18154; Mature: 18154

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALILSADNFEDTELLVPLYRLREAGYAVDVASEKAGSIHGKHGYEVPVNKLFNEVTPA
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCC
DYAVLVLPGGKAPAAIRNSPAVHEIARAFMSASKPVAAICHGPQILISAGLLKGRKATCY
CEEEEEEECCCCCHHHCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHH
ESVAPELRDAQAQYQDVEVIVDGNLITSRKPDDLPAFCRELVRMVKAAA
HHHCHHHHHHHHHHCCEEEEECCCEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKALILSADNFEDTELLVPLYRLREAGYAVDVASEKAGSIHGKHGYEVPVNKLFNEVTPA
CCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCC
DYAVLVLPGGKAPAAIRNSPAVHEIARAFMSASKPVAAICHGPQILISAGLLKGRKATCY
CEEEEEEECCCCCHHHCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHCCCCCCHHH
ESVAPELRDAQAQYQDVEVIVDGNLITSRKPDDLPAFCRELVRMVKAAA
HHHCHHHHHHHHHHCCEEEEECCCEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]