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The map label for this gene is hemL

Identifier: 197120288

GI number: 197120288

Start: 4465399

End: 4466682

Strand: Direct

Name: hemL

Synonym: Gbem_3927

Alternate gene names: 197120288

Gene position: 4465399-4466682 (Clockwise)

Preceding gene: 197120268

Following gene: 197120290

Centisome position: 96.76

GC content: 63.08

Gene sequence:

>1284_bases
ATGCAAAACAGCCGCTCGACCAAACTCTTTCAGCAGGCGCTTCAGTCCATCCCCGGCGGCGTCAACAGCCCGGTGCGCGC
CTTCAGGTCCGTTGGCTCCGATCCGCTCTTCATCAAAAAGGCCGCAGGCCCCCGCATCTACGATGAAGACGGCAACGCCT
TCATCGACTACGTCGGGTCCTGGGGACCGATGATCCTTGGGCACTGTCACCCGCAGGTGGTTTCCGCCATCAAGGCCGCC
GTCGACAACGGCGCCAGCTTCGGCGCGCCGACCGAGCTCGAGATCACCCTGGCCGAGATGGTGATCGACGCGGTTCCCTC
CATCGAGATGGTGCGCATGGTGAGCTCCGGTACCGAGGCGACCATGAGCGCCATCCGGCTCGCCCGCGGCTACACCGGCC
GCGACAACATCCTTAAGTTCTCCGGCTGCTACCACGGCCACTCCGACTCGCTTTTGGTCAAAGCCGGATCCGGCGCCGCC
ACCTTCGGCGTGCCCGACTCCCCCGGCGTCCCCGCCGACCTCGCCAAGCACACGCTGACCGCGACCTACAACGACCTGGA
CTCGGTCCGGGCGCTGGTAGCGGCCAACAAGGGAAGCATCGCCTGCATCATCGTGGAGCCTGTGGCTGGCAACATGGGGA
CCGTCCCCCCCAAGGAAGGATTCCTGGAGGGGCTTAGGAGCATATGCAGCGAGGAAGGAATCGTGCTGATCTTCGACGAG
GTGATGTCCGGCTTCAGGGTTGCCTACGGCGGCGTTCAGGAACTCTACGGCGTGACCCCCGACATGACCACGCTGGGCAA
GATCATCGGCGGTGGTCTGCCGGTGGGGGCGTTCGGCGGAAAAAAAGAAATCATGTCGCTTCTTTCACCGGCAGGGGGAG
TGTATCAGGCCGGGACCCTTTCTGGCAACCCCCTGGCCATGACCGCCGGCATCGAGACCTTGAAGCTCCTCAAGCAGCCC
GGTTTCTACCAGAAGCTGGAAGAAAAAAGCGCCTTCGTGGCGGAGGGGATCGCTAAGGCCGCCAAGGACGCCGGCTTCCC
GATCTACTCCACCCGGGTAGGCTCCATGTTCTGCGCCTTTTTCTCCAAAGATCCGGTCTACGACTGGGACAGCGCCGCCA
AGTGCGACACCAAGGCCTTCGCCGCCTACTTCAAGGCGATGCTGAATGAAGGTATTTACCTCGCGCCTTCGCAATTTGAG
ACGGCTTTCGTCGGCATCTCCCACAGCACCGAGGACCTGGAGCAGACCATCGCAGCCGCCGCCAAGTGCTTCAAGGCGCT
GTAG

Upstream 100 bases:

>100_bases
TCCCCCCCAAAACTTTTTAATTTCTTGACAGATAAGCCGGGCGCGTGCTACCAAACAGCCCATTATTTGTCAATTAACAG
CGAGTTAGGAGAATCAGCCC

Downstream 100 bases:

>100_bases
CGGGAATTGGCATTAACACTGACTCAGGGGATGCGGCAGCATAGTCTCCCCTCTCCCTGCTAATGCGCACAGTGCTGCCT
CAGATTGTTTATTAATTAAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MQNSRSTKLFQQALQSIPGGVNSPVRAFRSVGSDPLFIKKAAGPRIYDEDGNAFIDYVGSWGPMILGHCHPQVVSAIKAA
VDNGASFGAPTELEITLAEMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDNILKFSGCYHGHSDSLLVKAGSGAA
TFGVPDSPGVPADLAKHTLTATYNDLDSVRALVAANKGSIACIIVEPVAGNMGTVPPKEGFLEGLRSICSEEGIVLIFDE
VMSGFRVAYGGVQELYGVTPDMTTLGKIIGGGLPVGAFGGKKEIMSLLSPAGGVYQAGTLSGNPLAMTAGIETLKLLKQP
GFYQKLEEKSAFVAEGIAKAAKDAGFPIYSTRVGSMFCAFFSKDPVYDWDSAAKCDTKAFAAYFKAMLNEGIYLAPSQFE
TAFVGISHSTEDLEQTIAAAAKCFKAL

Sequences:

>Translated_427_residues
MQNSRSTKLFQQALQSIPGGVNSPVRAFRSVGSDPLFIKKAAGPRIYDEDGNAFIDYVGSWGPMILGHCHPQVVSAIKAA
VDNGASFGAPTELEITLAEMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDNILKFSGCYHGHSDSLLVKAGSGAA
TFGVPDSPGVPADLAKHTLTATYNDLDSVRALVAANKGSIACIIVEPVAGNMGTVPPKEGFLEGLRSICSEEGIVLIFDE
VMSGFRVAYGGVQELYGVTPDMTTLGKIIGGGLPVGAFGGKKEIMSLLSPAGGVYQAGTLSGNPLAMTAGIETLKLLKQP
GFYQKLEEKSAFVAEGIAKAAKDAGFPIYSTRVGSMFCAFFSKDPVYDWDSAAKCDTKAFAAYFKAMLNEGIYLAPSQFE
TAFVGISHSTEDLEQTIAAAAKCFKAL
>Mature_427_residues
MQNSRSTKLFQQALQSIPGGVNSPVRAFRSVGSDPLFIKKAAGPRIYDEDGNAFIDYVGSWGPMILGHCHPQVVSAIKAA
VDNGASFGAPTELEITLAEMVIDAVPSIEMVRMVSSGTEATMSAIRLARGYTGRDNILKFSGCYHGHSDSLLVKAGSGAA
TFGVPDSPGVPADLAKHTLTATYNDLDSVRALVAANKGSIACIIVEPVAGNMGTVPPKEGFLEGLRSICSEEGIVLIFDE
VMSGFRVAYGGVQELYGVTPDMTTLGKIIGGGLPVGAFGGKKEIMSLLSPAGGVYQAGTLSGNPLAMTAGIETLKLLKQP
GFYQKLEEKSAFVAEGIAKAAKDAGFPIYSTRVGSMFCAFFSKDPVYDWDSAAKCDTKAFAAYFKAMLNEGIYLAPSQFE
TAFVGISHSTEDLEQTIAAAAKCFKAL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=355, Percent_Identity=27.0422535211268, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI284507298, Length=274, Percent_Identity=27.7372262773723, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=59.0588235294118, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789016, Length=356, Percent_Identity=30.3370786516854, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788044, Length=317, Percent_Identity=31.5457413249211, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789759, Length=320, Percent_Identity=30, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787560, Length=396, Percent_Identity=29.5454545454545, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI145693181, Length=339, Percent_Identity=27.7286135693215, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786991, Length=380, Percent_Identity=26.8421052631579, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71992977, Length=368, Percent_Identity=27.7173913043478, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25144271, Length=356, Percent_Identity=26.9662921348315, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=199, Percent_Identity=31.6582914572864, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=413, Percent_Identity=26.3922518159806, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324432, Length=357, Percent_Identity=24.0896358543417, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356575, Length=316, Percent_Identity=28.1645569620253, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357415, Length=309, Percent_Identity=25.2427184466019, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_GEOBB (B5EFG4)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002140715.1
- ProteinModelPortal:   B5EFG4
- GeneID:   6780791
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_3927
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 44880; Mature: 44880

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSRSTKLFQQALQSIPGGVNSPVRAFRSVGSDPLFIKKAAGPRIYDEDGNAFIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCEEEEECCC
WGPMILGHCHPQVVSAIKAAVDNGASFGAPTELEITLAEMVIDAVPSIEMVRMVSSGTEA
CCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDNILKFSGCYHGHSDSLLVKAGSGAATFGVPDSPGVPADLAKHTLT
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHH
ATYNDLDSVRALVAANKGSIACIIVEPVAGNMGTVPPKEGFLEGLRSICSEEGIVLIFDE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHH
VMSGFRVAYGGVQELYGVTPDMTTLGKIIGGGLPVGAFGGKKEIMSLLSPAGGVYQAGTL
HHCCHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGIETLKLLKQPGFYQKLEEKSAFVAEGIAKAAKDAGFPIYSTRVGSMFCAF
CCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
FSKDPVYDWDSAAKCDTKAFAAYFKAMLNEGIYLAPSQFETAFVGISHSTEDLEQTIAAA
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCHHHHHHHHHHH
AKCFKAL
HHHHHCC
>Mature Secondary Structure
MQNSRSTKLFQQALQSIPGGVNSPVRAFRSVGSDPLFIKKAAGPRIYDEDGNAFIDYVGS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCEEEEECCC
WGPMILGHCHPQVVSAIKAAVDNGASFGAPTELEITLAEMVIDAVPSIEMVRMVSSGTEA
CCCCHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCHHHHHHHHCCHHH
TMSAIRLARGYTGRDNILKFSGCYHGHSDSLLVKAGSGAATFGVPDSPGVPADLAKHTLT
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHHHHHH
ATYNDLDSVRALVAANKGSIACIIVEPVAGNMGTVPPKEGFLEGLRSICSEEGIVLIFDE
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHH
VMSGFRVAYGGVQELYGVTPDMTTLGKIIGGGLPVGAFGGKKEIMSLLSPAGGVYQAGTL
HHCCHHHHHCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCC
SGNPLAMTAGIETLKLLKQPGFYQKLEEKSAFVAEGIAKAAKDAGFPIYSTRVGSMFCAF
CCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHH
FSKDPVYDWDSAAKCDTKAFAAYFKAMLNEGIYLAPSQFETAFVGISHSTEDLEQTIAAA
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCHHHHHHHHHHH
AKCFKAL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA