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The map label for this gene is napG [H]

Identifier: 197120236

GI number: 197120236

Start: 4411483

End: 4413069

Strand: Direct

Name: napG [H]

Synonym: Gbem_3875

Alternate gene names: 197120236

Gene position: 4411483-4413069 (Clockwise)

Preceding gene: 197120235

Following gene: 197120238

Centisome position: 95.59

GC content: 65.66

Gene sequence:

>1587_bases
ATGAAGTTTCCGCGCCCCCCCCGCATCTCGCAGCTTTTCTTCCTCCTCCTCTTCCTGATCCTTTTCCTGATGACCGAGTA
CCGCGGCAGCGACCGGATCGTCGCCGCGGTGAACGGGTTCTTCCGGGCCGATCCGCTCACCGCCGTTAGCAGCATGCTGG
CGGTGAAGGGTTACCTTCCGCTGCTCTTCCCCGGGCTCCTGGTGCTGGCCCTCTCCTTGATCCTCGGGCGTTTTTTCTGC
GGCTGGATCTGTCCGCTGGGAACCCTCCTCGACCTTGCGACCGGCAGGATAGGGAAGCTCGGCCCGGTCAGGGCGCTTCA
GGGGAGCGCCAAATACTGGCTTCTGCTCCCGTTACTCTTCGCTTCCCTGTTCGGGCTCAACCTGGCCGGGCTCTTCGATC
CTATCGCCATCCTGTTGCGCGCCCTCACCTTCTTCTTCCACCCGCTTTTGGGAGAGACGGCGCGCGGCGGCTGGAAATCC
CTCTACGGTTTGATCGAGGAGCGCCGGGACTTCCTCGACCCCGGCTACCACCTGCTGCGCGACTACCTCCTCCCTTTCCG
GGAGACGCTCTACCCACTGGCCTTCCTATCCGCGTTCCTCTTCTTCCTGGTCCTCTTCCTGGAGCGCTACGAAAAGCGCT
GCTGGTGCCGCCGGCTCTGCCCTTTGGGGACCCTGCTGGGCCTCGTCTCCCGCTTCGGCCCACTGCGGCGGGTCCCCTCG
AAGCTCTGCCCCGACTGCCGCGCCTGCCGCGAGCATTGCCCGACCTCCTTTGACCGCGAGCTGCTGCAGATGGACGAGTG
CATCCTCTGTCTTCAATGCGCGCTCAACTGCCCGTCGCAACGGGTCCGATTCGATTTTAGGGGGATGCGGCCTGAGGCGG
GGCCGGTAATGGAGCGGCGGGTCCTGCTGGGGGGGCTTCTAGGAGGCTTCGTCCTTGCCAGGACCTTCCGCTTCCGGGAG
CCGGAAGCCAAGGCGAGGATGCTGCGACCTCCCGGGGTGCGGGAGGAGGGGGAATTCCTGGAGAAATGCGTGCGCTGCGG
GGAGTGCATGAAGGTCTGCCTGAGAAGCGCCCTTTACCCTGCCTTCCTGCAGGCGGGGCCGGAGGCGCTCTACACCCCGG
TCCTGGTGCCGCGCCTGGGATACTGCGAGTACAACTGCACCCTCTGCGGCCAGGTCTGCCCCACCGGCGCCATCCCCGAT
CTTGCCGTCGAGGCGAAAAAGCGCGAGGTGATCGGCAAGGCGGTCTTCGACAAGAACCACTGCCTCCCCTTCGCCAAAAG
GGTGGACTGCATCGTCTGCGAGGAGCACTGCCCGATCCCGCAAAAGGCGATCCGCTCCGAACTGGTGGAGCTGACCGGTT
TCCAGGGGGAGAAGCTGCAGGTGAAGCAGCCGTACGTGGTGAGCGAGCTCTGCAACGGCTGCGGCATCTGCGAGAACGTC
TGCCCGCTGGAAGCAAAGTCCGCCATCGAGGTCTTCGCGGTGAAGGACCGCACCCCTCTGCCCCCTCCCGCCGACGCGCC
GTCCGCACCGGCCTCACAGCCCCCCGGCGGAGCCCCTCCCGATCCCTATGCAGGAGGTAACGGCTGA

Upstream 100 bases:

>100_bases
CGCTCTTCGGTTTGAAGCCCTCCGACATCGCCGTCACCAGGACGGCCTACCAGCGCGGGCTGGGGGAGATGAACCTGGAC
CGGATCAGGGTGGTCAAGGC

Downstream 100 bases:

>100_bases
CCCGCCAGGATAATGGTCGTAGACGAAGGGACCAGCGGGGGCGGCGCACGAAGGTATTGAGTTGGTTGCTAAGAAATGAA
TACGGCGCTTCCTCCGGGGG

Product: iron-sulfur cluster-binding oxidoreductase

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKFPRPPRISQLFFLLLFLILFLMTEYRGSDRIVAAVNGFFRADPLTAVSSMLAVKGYLPLLFPGLLVLALSLILGRFFC
GWICPLGTLLDLATGRIGKLGPVRALQGSAKYWLLLPLLFASLFGLNLAGLFDPIAILLRALTFFFHPLLGETARGGWKS
LYGLIEERRDFLDPGYHLLRDYLLPFRETLYPLAFLSAFLFFLVLFLERYEKRCWCRRLCPLGTLLGLVSRFGPLRRVPS
KLCPDCRACREHCPTSFDRELLQMDECILCLQCALNCPSQRVRFDFRGMRPEAGPVMERRVLLGGLLGGFVLARTFRFRE
PEAKARMLRPPGVREEGEFLEKCVRCGECMKVCLRSALYPAFLQAGPEALYTPVLVPRLGYCEYNCTLCGQVCPTGAIPD
LAVEAKKREVIGKAVFDKNHCLPFAKRVDCIVCEEHCPIPQKAIRSELVELTGFQGEKLQVKQPYVVSELCNGCGICENV
CPLEAKSAIEVFAVKDRTPLPPPADAPSAPASQPPGGAPPDPYAGGNG

Sequences:

>Translated_528_residues
MKFPRPPRISQLFFLLLFLILFLMTEYRGSDRIVAAVNGFFRADPLTAVSSMLAVKGYLPLLFPGLLVLALSLILGRFFC
GWICPLGTLLDLATGRIGKLGPVRALQGSAKYWLLLPLLFASLFGLNLAGLFDPIAILLRALTFFFHPLLGETARGGWKS
LYGLIEERRDFLDPGYHLLRDYLLPFRETLYPLAFLSAFLFFLVLFLERYEKRCWCRRLCPLGTLLGLVSRFGPLRRVPS
KLCPDCRACREHCPTSFDRELLQMDECILCLQCALNCPSQRVRFDFRGMRPEAGPVMERRVLLGGLLGGFVLARTFRFRE
PEAKARMLRPPGVREEGEFLEKCVRCGECMKVCLRSALYPAFLQAGPEALYTPVLVPRLGYCEYNCTLCGQVCPTGAIPD
LAVEAKKREVIGKAVFDKNHCLPFAKRVDCIVCEEHCPIPQKAIRSELVELTGFQGEKLQVKQPYVVSELCNGCGICENV
CPLEAKSAIEVFAVKDRTPLPPPADAPSAPASQPPGGAPPDPYAGGNG
>Mature_528_residues
MKFPRPPRISQLFFLLLFLILFLMTEYRGSDRIVAAVNGFFRADPLTAVSSMLAVKGYLPLLFPGLLVLALSLILGRFFC
GWICPLGTLLDLATGRIGKLGPVRALQGSAKYWLLLPLLFASLFGLNLAGLFDPIAILLRALTFFFHPLLGETARGGWKS
LYGLIEERRDFLDPGYHLLRDYLLPFRETLYPLAFLSAFLFFLVLFLERYEKRCWCRRLCPLGTLLGLVSRFGPLRRVPS
KLCPDCRACREHCPTSFDRELLQMDECILCLQCALNCPSQRVRFDFRGMRPEAGPVMERRVLLGGLLGGFVLARTFRFRE
PEAKARMLRPPGVREEGEFLEKCVRCGECMKVCLRSALYPAFLQAGPEALYTPVLVPRLGYCEYNCTLCGQVCPTGAIPD
LAVEAKKREVIGKAVFDKNHCLPFAKRVDCIVCEEHCPIPQKAIRSELVELTGFQGEKLQVKQPYVVSELCNGCGICENV
CPLEAKSAIEVFAVKDRTPLPPPADAPSAPASQPPGGAPPDPYAGGNG

Specific function: Involved in electron transfer (Probable) [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788533, Length=212, Percent_Identity=36.3207547169811, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787226, Length=272, Percent_Identity=29.0441176470588, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004494
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 58779; Mature: 58779

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
5.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPRPPRISQLFFLLLFLILFLMTEYRGSDRIVAAVNGFFRADPLTAVSSMLAVKGYLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCHHHCCHHHHHHHHHHHHHHHH
LLFPGLLVLALSLILGRFFCGWICPLGTLLDLATGRIGKLGPVRALQGSAKYWLLLPLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHH
ASLFGLNLAGLFDPIAILLRALTFFFHPLLGETARGGWKSLYGLIEERRDFLDPGYHLLR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
DYLLPFRETLYPLAFLSAFLFFLVLFLERYEKRCWCRRLCPLGTLLGLVSRFGPLRRVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KLCPDCRACREHCPTSFDRELLQMDECILCLQCALNCPSQRVRFDFRGMRPEAGPVMERR
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCCCHHHHHH
VLLGGLLGGFVLARTFRFREPEAKARMLRPPGVREEGEFLEKCVRCGECMKVCLRSALYP
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFLQAGPEALYTPVLVPRLGYCEYNCTLCGQVCPTGAIPDLAVEAKKREVIGKAVFDKNH
HHHHCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
CLPFAKRVDCIVCEEHCPIPQKAIRSELVELTGFQGEKLQVKQPYVVSELCNGCGICENV
CCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCC
CPLEAKSAIEVFAVKDRTPLPPPADAPSAPASQPPGGAPPDPYAGGNG
CCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFPRPPRISQLFFLLLFLILFLMTEYRGSDRIVAAVNGFFRADPLTAVSSMLAVKGYLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCHHHCCHHHHHHHHHHHHHHHH
LLFPGLLVLALSLILGRFFCGWICPLGTLLDLATGRIGKLGPVRALQGSAKYWLLLPLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCHHHHHHHHHH
ASLFGLNLAGLFDPIAILLRALTFFFHPLLGETARGGWKSLYGLIEERRDFLDPGYHLLR
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHH
DYLLPFRETLYPLAFLSAFLFFLVLFLERYEKRCWCRRLCPLGTLLGLVSRFGPLRRVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
KLCPDCRACREHCPTSFDRELLQMDECILCLQCALNCPSQRVRFDFRGMRPEAGPVMERR
HHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHCCCCCCCCHHHHHH
VLLGGLLGGFVLARTFRFREPEAKARMLRPPGVREEGEFLEKCVRCGECMKVCLRSALYP
HHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AFLQAGPEALYTPVLVPRLGYCEYNCTLCGQVCPTGAIPDLAVEAKKREVIGKAVFDKNH
HHHHCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
CLPFAKRVDCIVCEEHCPIPQKAIRSELVELTGFQGEKLQVKQPYVVSELCNGCGICENV
CCCHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCC
CPLEAKSAIEVFAVKDRTPLPPPADAPSAPASQPPGGAPPDPYAGGNG
CCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]