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The map label for this gene is zraS [H]

Identifier: 197120179

GI number: 197120179

Start: 4357181

End: 4358587

Strand: Direct

Name: zraS [H]

Synonym: Gbem_3818

Alternate gene names: 197120179

Gene position: 4357181-4358587 (Clockwise)

Preceding gene: 197120177

Following gene: 197120180

Centisome position: 94.41

GC content: 60.27

Gene sequence:

>1407_bases
ATGAAACTCAATACCAAGCTGGTGATGATTATGCTCACCATGCTTATCGTGGCGACGGCTATGCTCTTCGTCCTGAATCA
GGTCAGCCAGAACGACCTGGTGGGGGAGATCCAGGAGAGTTCCACCATGGTGTCGAAAGCCATTCAGCTCAGCGTGGAAG
ACCTGACCTCCGAGGTCGAATCATCGCGCCTGACCGAGTACCTCCAGCAGGCGAAGGGCAAAGGGGTCAACGAGATCAAC
ATCATCAACAACGAGGGGGAGATCATCAACTCCTCGGATCCCGCCCAAGTCGGTAAAAAACGCGAAATCAAAAAGCTGGA
GAAGGGGCTGCGGGCCTCGCGCCGCGGCGGCGGCGGGGGGTCGCTCAAGCCGTACGACCTGGTGGTGCCTGTCATCGTGG
GCGACGAGCAGCTGGGTTACGTGCAGGTGAACCTCCTTCTCGACAACATACGCGACATCCAGCACGCCAACTTCGTCAAG
CGCCTGGCTGCGACCACCATGGTGTTCCTGATGGGGATGATACTGATCATCTTCCTCGCGCGCCGCTACACCTCGCCGAT
ACACCGGCTGGCTACGGGGGTGAAGCACGTCTCCGGGGGGGACCTGAGCGTCACCTTCCAGGGGGGGAGCGGCGACGAGA
TCGGCGAGCTTGCCGAGAACCTGAACGAGATGGTGGAAAAGCTGAAAGAAAAGGAACTGCTCGAAAAGCGGCTCTACGAG
GCGGAGCACCTCTCCAAGGTGGGGCAGCTGGCCGCGGGGATCGCGCACGAGATCAGGAACCCGCTCAATTACATAAGCCT
CGCCATCGACCACCTGAAGAGCGAATTCCTCCCCTCCTGCCCCGAAAAGGCCCAGGATCTGGAGTCGATCGCCAACAACA
TCAAGGAAGAGGTGCGCAAGGCGAACTACATGGTGCTCAATTTCATGAATTACGGCCGTCCCTTGAAGCTGCGGCTGCAG
CGGGTATGTTACCCTGAACTCGTGGACAAGGCGATGCAACTCATGAAAGACCGGCTCGACGAAAGGGGAATCGAAGTGGT
GCGGGAAATACCCGAGGATCTGCCGCCGATGCTGGCGGACCCGGAGCTGATGCGCAACTGCCTGTGCAACTTCATCAGTA
ACAGCACCCAGGCGATGCCGGAGGGGGGAAAGTTCACCATCGGCGCGAGCCTTGCCCCCGAAACCGGCCAGTTCTGCCTC
ACCTTCAGCGACGAGGGGACGGGGATCGAACCGCAGGACCTGGAGAAGGTGTTTCAGCCCTACTTCACCACCAAGGAGGC
CGGGATCGGCCTCGGGCTCGCCATCACCGAACGGATCGTGAGGGAGCACGGCGGCAGCATCGCGGTTCGAAGCACGAAAG
GGGAAGGGACCACCTTCTCGGTCACCCTCCCGGCGGCAACGGCATAA

Upstream 100 bases:

>100_bases
TTTTCATCTAAGCCTGCCGGCGCTGGACATTCCTCTTGCCCAGCTCAAGTGAGCGCAGTATACTTCCGAGCTTAGTGAAA
ACTCATACGGAAAGGACCTC

Downstream 100 bases:

>100_bases
AGCACCGCCCGGCTCCCAGGGCACGGGAGCAAAAAGAGCAAGGAGTAGCAATGATCGGCAGCATCCTGATAGTAGACGAC
GAGAAGGGGCAGCGGGACAT

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKLNTKLVMIMLTMLIVATAMLFVLNQVSQNDLVGEIQESSTMVSKAIQLSVEDLTSEVESSRLTEYLQQAKGKGVNEIN
IINNEGEIINSSDPAQVGKKREIKKLEKGLRASRRGGGGGSLKPYDLVVPVIVGDEQLGYVQVNLLLDNIRDIQHANFVK
RLAATTMVFLMGMILIIFLARRYTSPIHRLATGVKHVSGGDLSVTFQGGSGDEIGELAENLNEMVEKLKEKELLEKRLYE
AEHLSKVGQLAAGIAHEIRNPLNYISLAIDHLKSEFLPSCPEKAQDLESIANNIKEEVRKANYMVLNFMNYGRPLKLRLQ
RVCYPELVDKAMQLMKDRLDERGIEVVREIPEDLPPMLADPELMRNCLCNFISNSTQAMPEGGKFTIGASLAPETGQFCL
TFSDEGTGIEPQDLEKVFQPYFTTKEAGIGLGLAITERIVREHGGSIAVRSTKGEGTTFSVTLPAATA

Sequences:

>Translated_468_residues
MKLNTKLVMIMLTMLIVATAMLFVLNQVSQNDLVGEIQESSTMVSKAIQLSVEDLTSEVESSRLTEYLQQAKGKGVNEIN
IINNEGEIINSSDPAQVGKKREIKKLEKGLRASRRGGGGGSLKPYDLVVPVIVGDEQLGYVQVNLLLDNIRDIQHANFVK
RLAATTMVFLMGMILIIFLARRYTSPIHRLATGVKHVSGGDLSVTFQGGSGDEIGELAENLNEMVEKLKEKELLEKRLYE
AEHLSKVGQLAAGIAHEIRNPLNYISLAIDHLKSEFLPSCPEKAQDLESIANNIKEEVRKANYMVLNFMNYGRPLKLRLQ
RVCYPELVDKAMQLMKDRLDERGIEVVREIPEDLPPMLADPELMRNCLCNFISNSTQAMPEGGKFTIGASLAPETGQFCL
TFSDEGTGIEPQDLEKVFQPYFTTKEAGIGLGLAITERIVREHGGSIAVRSTKGEGTTFSVTLPAATA
>Mature_468_residues
MKLNTKLVMIMLTMLIVATAMLFVLNQVSQNDLVGEIQESSTMVSKAIQLSVEDLTSEVESSRLTEYLQQAKGKGVNEIN
IINNEGEIINSSDPAQVGKKREIKKLEKGLRASRRGGGGGSLKPYDLVVPVIVGDEQLGYVQVNLLLDNIRDIQHANFVK
RLAATTMVFLMGMILIIFLARRYTSPIHRLATGVKHVSGGDLSVTFQGGSGDEIGELAENLNEMVEKLKEKELLEKRLYE
AEHLSKVGQLAAGIAHEIRNPLNYISLAIDHLKSEFLPSCPEKAQDLESIANNIKEEVRKANYMVLNFMNYGRPLKLRLQ
RVCYPELVDKAMQLMKDRLDERGIEVVREIPEDLPPMLADPELMRNCLCNFISNSTQAMPEGGKFTIGASLAPETGQFCL
TFSDEGTGIEPQDLEKVFQPYFTTKEAGIGLGLAITERIVREHGGSIAVRSTKGEGTTFSVTLPAATA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=35.2941176470588, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788549, Length=303, Percent_Identity=30.6930693069307, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1789149, Length=368, Percent_Identity=25.8152173913043, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081816, Length=333, Percent_Identity=26.4264264264264, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=23.7762237762238, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=24.223602484472, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1790300, Length=258, Percent_Identity=27.5193798449612, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=293, Percent_Identity=23.2081911262799, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=321, Percent_Identity=23.6760124610592, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=232, Percent_Identity=28.0172413793103, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=27.1551724137931, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=22.4215246636771, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51622; Mature: 51622

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNTKLVMIMLTMLIVATAMLFVLNQVSQNDLVGEIQESSTMVSKAIQLSVEDLTSEVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRLTEYLQQAKGKGVNEINIINNEGEIINSSDPAQVGKKREIKKLEKGLRASRRGGGGG
HHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SLKPYDLVVPVIVGDEQLGYVQVNLLLDNIRDIQHANFVKRLAATTMVFLMGMILIIFLA
CCCCHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYTSPIHRLATGVKHVSGGDLSVTFQGGSGDEIGELAENLNEMVEKLKEKELLEKRLYE
HHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEHLSKVGQLAAGIAHEIRNPLNYISLAIDHLKSEFLPSCPEKAQDLESIANNIKEEVRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ANYMVLNFMNYGRPLKLRLQRVCYPELVDKAMQLMKDRLDERGIEVVREIPEDLPPMLAD
HHHHEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PELMRNCLCNFISNSTQAMPEGGKFTIGASLAPETGQFCLTFSDEGTGIEPQDLEKVFQP
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
YFTTKEAGIGLGLAITERIVREHGGSIAVRSTKGEGTTFSVTLPAATA
HHCCHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCC
>Mature Secondary Structure
MKLNTKLVMIMLTMLIVATAMLFVLNQVSQNDLVGEIQESSTMVSKAIQLSVEDLTSEVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSRLTEYLQQAKGKGVNEINIINNEGEIINSSDPAQVGKKREIKKLEKGLRASRRGGGGG
HHHHHHHHHHHHCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
SLKPYDLVVPVIVGDEQLGYVQVNLLLDNIRDIQHANFVKRLAATTMVFLMGMILIIFLA
CCCCHHEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRYTSPIHRLATGVKHVSGGDLSVTFQGGSGDEIGELAENLNEMVEKLKEKELLEKRLYE
HHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEHLSKVGQLAAGIAHEIRNPLNYISLAIDHLKSEFLPSCPEKAQDLESIANNIKEEVRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ANYMVLNFMNYGRPLKLRLQRVCYPELVDKAMQLMKDRLDERGIEVVREIPEDLPPMLAD
HHHHEEEHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PELMRNCLCNFISNSTQAMPEGGKFTIGASLAPETGQFCLTFSDEGTGIEPQDLEKVFQP
HHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHH
YFTTKEAGIGLGLAITERIVREHGGSIAVRSTKGEGTTFSVTLPAATA
HHCCHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]