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The map label for this gene is glcF-2 [H]

Identifier: 197119277

GI number: 197119277

Start: 3351548

End: 3352789

Strand: Reverse

Name: glcF-2 [H]

Synonym: Gbem_2905

Alternate gene names: 197119277

Gene position: 3352789-3351548 (Counterclockwise)

Preceding gene: 197119278

Following gene: 197119276

Centisome position: 72.65

GC content: 64.01

Gene sequence:

>1242_bases
ATGGATTACGTCAAGCTGATCAACTGCATGCGCTGCGGCATGTGCCTGCCGCACTGCCCGACCTATAAGGAGACCTTCCT
GGAAACCGCCTCGCCGCGCGGACGCGTGGCGCTGGTGCGCAAGCTCCAGGAAGGGGAGCTGGTGGAAAGCGAGAAGTTCC
TGGAGTACGTCTCTCTCTGCCTTGACTGCCAGGCCTGCGCCTCCGCCTGCCCCTGCGGCGTCAACGCCGGCGAACTGGTG
GCCGAGTTCCGCTGCGAGAGCAAGAAGGAGAAGGGGCTCTCCATGATGGAGGACCTGGTCCTGAGAAAGCTGGTGCCGCA
CCCGGACCGCCTGGAGGCCGCTACGGCGCCGCTTCGGCTCTACCAGAAAACCGGCCTGCAGCAGGTGGTCCGCTCGCTGG
GGCTCTTGAAACTGTTCCCTGAGGCGCTGGGACGCATGGAAGGGCTTCTGCCTAAGCTCCCCGACGCGCCGCTGCGCCAG
ACCATCAGCGAGGTGACGCCGGCGGCTGGAAAAGAGAAGGGGACCGCGGGCTTTTTCCTTGGCTGCGTCATGAGCCTCAT
CTTCAGCGAGGCGAGCCTTGCCACGGTGCAGCTGCTCTCGACTTTGGGATACCGGGTGGTGACCCCCAAGGCTCAGAAGT
GCTGCGGCGCTCCCAACATGCTGGGGCACGATCTGGACGGGCTCAAGGAGGCCGCTAGCTTCAACACCGACCTCTTCGAC
TCCTTCGACCTCGATTTCGTGGTCACCGACTGCGGCGGCTGCGGGGCCGAGCTGAAGAAGTACGGCCAGCACCTGGACGG
CGAGCGCAAAGCCGAGGAATTCAGCGCCAGCGTGCGCGACATCTCCGAAGTCCTCTTCGCCGAGTCGGAGGCTTTGGCCG
CAAAACTCAAACCCCTGCCGTTAAAGGTCACCTACCACGACCCCTGCCACATCGCCCACTGCCAGGGCATCCGCAGCCAG
CCGCGCGCGCTGATCAAGCTGATACCCGGAATCGACTTCCGCGAGCTCCCCGAGGCGGATGCCTGCTGCGGGTCGGCGGG
TACCTACAACATCGAGAAGCCGGAGATGGCGGACCTGGTGCTGTCGCGCAAGGTGCAAAACGTGCAGAAGACGGGAGCCG
AATACCTCGTGACCAGCAACCCCGGGTGCCTCTTGCAGCTCAAGAAGGCGCTCTCGGAGGCCGAGCCGCAGGTAAGGGTG
ATTCATCTCACCGAACTGCTTCAGATGAGCATGAATGCGTGA

Upstream 100 bases:

>100_bases
CAAGGGGATACTGAACCCTGGGAAAATCTTTAGTCTGACTTCGGACTAGTCCGACCCGTCTGACCTGTCCGACGCCGTTT
CTTCAACAAGAGGATGCACC

Downstream 100 bases:

>100_bases
GGGTAGGGGAGGTTACCTCTTCATGCCGTATACGGAATTTTCCCGGCTGCCAGATTGGTCAAAAATATTTACCAGAAAAC
CTATTGCCTTCCTCTTACAT

Product: D-lactate/glycolate dehydrogenase iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MDYVKLINCMRCGMCLPHCPTYKETFLETASPRGRVALVRKLQEGELVESEKFLEYVSLCLDCQACASACPCGVNAGELV
AEFRCESKKEKGLSMMEDLVLRKLVPHPDRLEAATAPLRLYQKTGLQQVVRSLGLLKLFPEALGRMEGLLPKLPDAPLRQ
TISEVTPAAGKEKGTAGFFLGCVMSLIFSEASLATVQLLSTLGYRVVTPKAQKCCGAPNMLGHDLDGLKEAASFNTDLFD
SFDLDFVVTDCGGCGAELKKYGQHLDGERKAEEFSASVRDISEVLFAESEALAAKLKPLPLKVTYHDPCHIAHCQGIRSQ
PRALIKLIPGIDFRELPEADACCGSAGTYNIEKPEMADLVLSRKVQNVQKTGAEYLVTSNPGCLLQLKKALSEAEPQVRV
IHLTELLQMSMNA

Sequences:

>Translated_413_residues
MDYVKLINCMRCGMCLPHCPTYKETFLETASPRGRVALVRKLQEGELVESEKFLEYVSLCLDCQACASACPCGVNAGELV
AEFRCESKKEKGLSMMEDLVLRKLVPHPDRLEAATAPLRLYQKTGLQQVVRSLGLLKLFPEALGRMEGLLPKLPDAPLRQ
TISEVTPAAGKEKGTAGFFLGCVMSLIFSEASLATVQLLSTLGYRVVTPKAQKCCGAPNMLGHDLDGLKEAASFNTDLFD
SFDLDFVVTDCGGCGAELKKYGQHLDGERKAEEFSASVRDISEVLFAESEALAAKLKPLPLKVTYHDPCHIAHCQGIRSQ
PRALIKLIPGIDFRELPEADACCGSAGTYNIEKPEMADLVLSRKVQNVQKTGAEYLVTSNPGCLLQLKKALSEAEPQVRV
IHLTELLQMSMNA
>Mature_413_residues
MDYVKLINCMRCGMCLPHCPTYKETFLETASPRGRVALVRKLQEGELVESEKFLEYVSLCLDCQACASACPCGVNAGELV
AEFRCESKKEKGLSMMEDLVLRKLVPHPDRLEAATAPLRLYQKTGLQQVVRSLGLLKLFPEALGRMEGLLPKLPDAPLRQ
TISEVTPAAGKEKGTAGFFLGCVMSLIFSEASLATVQLLSTLGYRVVTPKAQKCCGAPNMLGHDLDGLKEAASFNTDLFD
SFDLDFVVTDCGGCGAELKKYGQHLDGERKAEEFSASVRDISEVLFAESEALAAKLKPLPLKVTYHDPCHIAHCQGIRSQ
PRALIKLIPGIDFRELPEADACCGSAGTYNIEKPEMADLVLSRKVQNVQKTGAEYLVTSNPGCLLQLKKALSEAEPQVRV
IHLTELLQMSMNA

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=413, Percent_Identity=27.3607748184019, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=26.3598326359833, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788576, Length=406, Percent_Identity=23.6453201970443, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45115; Mature: 45115

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYVKLINCMRCGMCLPHCPTYKETFLETASPRGRVALVRKLQEGELVESEKFLEYVSLC
CCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LDCQACASACPCGVNAGELVAEFRCESKKEKGLSMMEDLVLRKLVPHPDRLEAATAPLRL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YQKTGLQQVVRSLGLLKLFPEALGRMEGLLPKLPDAPLRQTISEVTPAAGKEKGTAGFFL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
GCVMSLIFSEASLATVQLLSTLGYRVVTPKAQKCCGAPNMLGHDLDGLKEAASFNTDLFD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHCCCCHHC
SFDLDFVVTDCGGCGAELKKYGQHLDGERKAEEFSASVRDISEVLFAESEALAAKLKPLP
CCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LKVTYHDPCHIAHCQGIRSQPRALIKLIPGIDFRELPEADACCGSAGTYNIEKPEMADLV
EEEEECCCCCHHHHCCCCCHHHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCHHHHHH
LSRKVQNVQKTGAEYLVTSNPGCLLQLKKALSEAEPQVRVIHLTELLQMSMNA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure
MDYVKLINCMRCGMCLPHCPTYKETFLETASPRGRVALVRKLQEGELVESEKFLEYVSLC
CCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
LDCQACASACPCGVNAGELVAEFRCESKKEKGLSMMEDLVLRKLVPHPDRLEAATAPLRL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YQKTGLQQVVRSLGLLKLFPEALGRMEGLLPKLPDAPLRQTISEVTPAAGKEKGTAGFFL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
GCVMSLIFSEASLATVQLLSTLGYRVVTPKAQKCCGAPNMLGHDLDGLKEAASFNTDLFD
HHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCHHHHHHHHHCCCCHHC
SFDLDFVVTDCGGCGAELKKYGQHLDGERKAEEFSASVRDISEVLFAESEALAAKLKPLP
CCCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LKVTYHDPCHIAHCQGIRSQPRALIKLIPGIDFRELPEADACCGSAGTYNIEKPEMADLV
EEEEECCCCCHHHHCCCCCHHHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCHHHHHH
LSRKVQNVQKTGAEYLVTSNPGCLLQLKKALSEAEPQVRVIHLTELLQMSMNA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]