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The map label for this gene is mdh

Identifier: 197119272

GI number: 197119272

Start: 3344407

End: 3345360

Strand: Reverse

Name: mdh

Synonym: Gbem_2900

Alternate gene names: 197119272

Gene position: 3345360-3344407 (Counterclockwise)

Preceding gene: 197119273

Following gene: 197119271

Centisome position: 72.49

GC content: 59.12

Gene sequence:

>954_bases
ATGGCACGTAAGAAAATCGCACTTATCGGTGGTGGACAGATTGGCGGCGTACTTGCTCAGCTTGCAGCTCTGCGCGAACT
GGGTGACGTAGTAATGTTCGACATCGTCGAAGGCCTGCCGCAGGGCAAGATGCTCGACATCGCCGAAGTCGGCTCTGTCG
ACGGCTTCGACTGCAACCTGAAAGGGACCAACAGCTACGAGGACATCAAAGGCGCCGACGTGGTCATCGTCACCGCAGGC
CTTCCCCGCAAACCGGGCATGAGCCGCGACGACCTGATCGAGGTCAACTCCAAGATCATGACCTCCGTGGCTGAGGGGAT
CAAGGCCAACGCACCCAACGCGTTCGTCATCGTCATCTCCAACCCGCTCGACGCCATGGTTACCCTCTGCCAGAAGATCA
CCGGCTTCCCCTACAACCGCGTCATCGGCCAGGCCGGCGTGCTCGACTCCGCACGCTTCAAAACCTTCATCGCTTGGGAA
CTGGGCGTTTCCGTGAAAGACGTCAACGCCATGACCCTGGGCGGCCACGGCGACGACATGGTGCCGCTGGTACGTTACGC
CTCTGTGAACGGGATCCCCGTCATGGAACTCCTCGAGAAGAAATACAAAGACAAAGCCAAGGCTAAAGAAGTCATGGAAG
CCATGGTCAAGAGGACCCGCGGTGCAGGCGGCGAGGTTGTTGCTCTCCTCAAAACCGGTTCCGCATTCTACTCCCCGGCT
TCCTCCGCCATCCAGATGGCTGAATCGATCCTCAAGGATCAAAAGCGCGTCCTCCCGACCTGCGCTCACCTGAACGGCGA
GTTCGGCGTGAAAGGCTTCTACGTCGGCGTTCCCTGCGTGCTGGGCGAGAACGGCGTCGAGCAGATCCTCGAGTTCGAAC
TGGACGCAGAAGAGCAGGCGATGATGGACAAGTCCGTCGCAGCCGTCAAGGAACTCGTAGGTTCCATGAAGTAA

Upstream 100 bases:

>100_bases
TGCGCCCGAGCGCAACGCTGAACGCCATCATCAACTCGATCGTATAAAGCTTTCGCTGCACCAGGTAGATAATCTCAATT
TAATTACGGAGGAAGAAAAA

Downstream 100 bases:

>100_bases
TCGGAGGCTGAACACGCTTTGCCAGGGGGTAAGGAGGTGTCCCCTCCTTACCCCTTTTTTTCGAGGCAGAGCCAAGGTGC
CGGTCAGCGTCGCTTGCATT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MARKKIALIGGGQIGGVLAQLAALRELGDVVMFDIVEGLPQGKMLDIAEVGSVDGFDCNLKGTNSYEDIKGADVVIVTAG
LPRKPGMSRDDLIEVNSKIMTSVAEGIKANAPNAFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSARFKTFIAWE
LGVSVKDVNAMTLGGHGDDMVPLVRYASVNGIPVMELLEKKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
SSAIQMAESILKDQKRVLPTCAHLNGEFGVKGFYVGVPCVLGENGVEQILEFELDAEEQAMMDKSVAAVKELVGSMK

Sequences:

>Translated_317_residues
MARKKIALIGGGQIGGVLAQLAALRELGDVVMFDIVEGLPQGKMLDIAEVGSVDGFDCNLKGTNSYEDIKGADVVIVTAG
LPRKPGMSRDDLIEVNSKIMTSVAEGIKANAPNAFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSARFKTFIAWE
LGVSVKDVNAMTLGGHGDDMVPLVRYASVNGIPVMELLEKKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
SSAIQMAESILKDQKRVLPTCAHLNGEFGVKGFYVGVPCVLGENGVEQILEFELDAEEQAMMDKSVAAVKELVGSMK
>Mature_316_residues
ARKKIALIGGGQIGGVLAQLAALRELGDVVMFDIVEGLPQGKMLDIAEVGSVDGFDCNLKGTNSYEDIKGADVVIVTAGL
PRKPGMSRDDLIEVNSKIMTSVAEGIKANAPNAFVIVISNPLDAMVTLCQKITGFPYNRVIGQAGVLDSARFKTFIAWEL
GVSVKDVNAMTLGGHGDDMVPLVRYASVNGIPVMELLEKKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPAS
SAIQMAESILKDQKRVLPTCAHLNGEFGVKGFYVGVPCVLGENGVEQILEFELDAEEQAMMDKSVAAVKELVGSMK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI221136809, Length=319, Percent_Identity=31.6614420062696, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI5031857, Length=307, Percent_Identity=31.9218241042345, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI260099723, Length=307, Percent_Identity=31.9218241042345, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=31.6993464052288, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=31.6993464052288, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI15082234, Length=317, Percent_Identity=28.391167192429, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI291575128, Length=319, Percent_Identity=27.8996865203762, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4557032, Length=319, Percent_Identity=27.8996865203762, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI207028494, Length=191, Percent_Identity=35.0785340314136, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=31.9634703196347, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI260099725, Length=212, Percent_Identity=31.6037735849057, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI21735621, Length=322, Percent_Identity=25.4658385093168, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789632, Length=332, Percent_Identity=28.9156626506024, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=286, Percent_Identity=23.7762237762238, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17554310, Length=314, Percent_Identity=29.9363057324841, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=27.7591973244147, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=282, Percent_Identity=28.3687943262411, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=177, Percent_Identity=31.638418079096, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136226, Length=322, Percent_Identity=27.0186335403727, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24647881, Length=292, Percent_Identity=29.4520547945205, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24663599, Length=322, Percent_Identity=28.2608695652174, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=26.0726072607261, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24663595, Length=265, Percent_Identity=29.4339622641509, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_GEOBB (B5EIU8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139699.1
- ProteinModelPortal:   B5EIU8
- GeneID:   6779753
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2900
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   PRK06223
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33729; Mature: 33598

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALIGGGQIGGVLAQLAALRELGDVVMFDIVEGLPQGKMLDIAEVGSVDGFDCNL
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCC
KGTNSYEDIKGADVVIVTAGLPRKPGMSRDDLIEVNSKIMTSVAEGIKANAPNAFVIVIS
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NPLDAMVTLCQKITGFPYNRVIGQAGVLDSARFKTFIAWELGVSVKDVNAMTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHEEEEEEECCEEECCCEEEECCCCCCH
VPLVRYASVNGIPVMELLEKKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCH
SSAIQMAESILKDQKRVLPTCAHLNGEFGVKGFYVGVPCVLGENGVEQILEFELDAEEQA
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHCCCCHHHH
MMDKSVAAVKELVGSMK
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKKIALIGGGQIGGVLAQLAALRELGDVVMFDIVEGLPQGKMLDIAEVGSVDGFDCNL
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHCCCCCCCCCC
KGTNSYEDIKGADVVIVTAGLPRKPGMSRDDLIEVNSKIMTSVAEGIKANAPNAFVIVIS
CCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
NPLDAMVTLCQKITGFPYNRVIGQAGVLDSARFKTFIAWELGVSVKDVNAMTLGGHGDDM
CCHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHEEEEEEECCEEECCCEEEECCCCCCH
VPLVRYASVNGIPVMELLEKKYKDKAKAKEVMEAMVKRTRGAGGEVVALLKTGSAFYSPA
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCH
SSAIQMAESILKDQKRVLPTCAHLNGEFGVKGFYVGVPCVLGENGVEQILEFELDAEEQA
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHCCCCHHHH
MMDKSVAAVKELVGSMK
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA