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The map label for this gene is pfk-3 [H]

Identifier: 197119193

GI number: 197119193

Start: 3246050

End: 3247222

Strand: Reverse

Name: pfk-3 [H]

Synonym: Gbem_2820

Alternate gene names: 197119193

Gene position: 3247222-3246050 (Counterclockwise)

Preceding gene: 197119194

Following gene: 197119192

Centisome position: 70.36

GC content: 62.75

Gene sequence:

>1173_bases
ATGGGTGACAGACAAAAGACGGTGGGGGTGCTGACTGGCGGGGGCGATGTGCCTGGGCTGAACCCTGCCCTGAAAACCTT
GGTGACCCGCGCCGTAGAGAACGGCTACCGGGTCCTCGGCATCCGGCGCGGCTGGGGCGGACTCCTAGCCTACAACCGCG
ACGACCCGCACTCCGGCGAGAGCACCCTGATGCCTTTGGACCGGCACTCGGTTCGCACCGTAGACCGCTCCGGCGGCACC
TTCCTGCACACAAGCCGCACCAACCCGGGCAAAGTGGCGACCGCCGACGTGCCTGAGTTCCTGCGCGGCGCAGACTTCGA
TCCGCTTAACCCCGGGCATCACGATTTCACCCAACATGTTCTCGCCAACCTGGAGCACCTGGGGGTCGACTCCTTGATCG
CCATCGGCGGCGACGATACCTTAAGCTACGCCGAACGCCTCTTCCGCGAGGGGGTCCCGGTGGTCGCCATTCCCAAGACC
ATGGACAACGACGTTTTCGGCACCGACTACTGCATCGGCTTTTCCACGGCCGTGACCCGCAGCGTCAACTTCATCCACAA
CCTGAGGACCAGCGCCGGCTCCCATGAGAGGATTGCGGTGGTCGAGCTCTTCGGGCGCAACTCGGGCGAAACCTCCCTCA
TCTCCGCGTACCTTGCCGGGGTCGATCGCGCCCTCATCTCCGAGGTTCCCTTCGATGTCGAAAGGCTCGCCCTCCTCTTG
CAGGAAGACAAAAGATCAAATCGCAGCAATTACGCCATGGTGACCGTATCCGAAGGGGCATCGATGATCGGCGGGCAGAC
CGTCGAGTACGGCCAGGAGGATGCCTACGGCCACAAGAAGCTGGGGGGCATCGGCGTGGTAGTGAGCGAGGCGGTGAAGG
GGCTCACCGGTTCGCACATCATCTACCAGCAGCTCTCTTACCTCATGCGCAGCGGCGCCCCTGACTCCCTCGACCTCATG
GTCGCCTACAACTACGCCAATATGACCATGGATCTGATCAGCGAGGGGATGTCGGGAAGGATGGTATCCCTGCAGGGAGG
GACCTACCGGCACATCCCGATCAGCACCATCATGCAAGGCGAGAAGCGGGTAGACGTGGCCGAACTCTACGACCTCGCGG
AGTACCGCCCCAAGGTGCGCCACGTACTCGGCAAACCGATGTTCCTGTACTAA

Upstream 100 bases:

>100_bases
AGGCATCTCGCCGACGACAAGAAAGCTTGAAATGCACATCTCCACCTATATAATAGTGAGCGTTCTCTAATTCCATTAAT
CACTAGAAAGGAGTCTGCTC

Downstream 100 bases:

>100_bases
TCCCAGACAAACATATGATAGAGATCGACGGCAGCGTCGGTGAGGGGGGAGGGCAAGTGCTGCGCAGCGCGCTCAGCCTC
TCCTGCCTCACCGGCCAAGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MGDRQKTVGVLTGGGDVPGLNPALKTLVTRAVENGYRVLGIRRGWGGLLAYNRDDPHSGESTLMPLDRHSVRTVDRSGGT
FLHTSRTNPGKVATADVPEFLRGADFDPLNPGHHDFTQHVLANLEHLGVDSLIAIGGDDTLSYAERLFREGVPVVAIPKT
MDNDVFGTDYCIGFSTAVTRSVNFIHNLRTSAGSHERIAVVELFGRNSGETSLISAYLAGVDRALISEVPFDVERLALLL
QEDKRSNRSNYAMVTVSEGASMIGGQTVEYGQEDAYGHKKLGGIGVVVSEAVKGLTGSHIIYQQLSYLMRSGAPDSLDLM
VAYNYANMTMDLISEGMSGRMVSLQGGTYRHIPISTIMQGEKRVDVAELYDLAEYRPKVRHVLGKPMFLY

Sequences:

>Translated_390_residues
MGDRQKTVGVLTGGGDVPGLNPALKTLVTRAVENGYRVLGIRRGWGGLLAYNRDDPHSGESTLMPLDRHSVRTVDRSGGT
FLHTSRTNPGKVATADVPEFLRGADFDPLNPGHHDFTQHVLANLEHLGVDSLIAIGGDDTLSYAERLFREGVPVVAIPKT
MDNDVFGTDYCIGFSTAVTRSVNFIHNLRTSAGSHERIAVVELFGRNSGETSLISAYLAGVDRALISEVPFDVERLALLL
QEDKRSNRSNYAMVTVSEGASMIGGQTVEYGQEDAYGHKKLGGIGVVVSEAVKGLTGSHIIYQQLSYLMRSGAPDSLDLM
VAYNYANMTMDLISEGMSGRMVSLQGGTYRHIPISTIMQGEKRVDVAELYDLAEYRPKVRHVLGKPMFLY
>Mature_389_residues
GDRQKTVGVLTGGGDVPGLNPALKTLVTRAVENGYRVLGIRRGWGGLLAYNRDDPHSGESTLMPLDRHSVRTVDRSGGTF
LHTSRTNPGKVATADVPEFLRGADFDPLNPGHHDFTQHVLANLEHLGVDSLIAIGGDDTLSYAERLFREGVPVVAIPKTM
DNDVFGTDYCIGFSTAVTRSVNFIHNLRTSAGSHERIAVVELFGRNSGETSLISAYLAGVDRALISEVPFDVERLALLLQ
EDKRSNRSNYAMVTVSEGASMIGGQTVEYGQEDAYGHKKLGGIGVVVSEAVKGLTGSHIIYQQLSYLMRSGAPDSLDLMV
AYNYANMTMDLISEGMSGRMVSLQGGTYRHIPISTIMQGEKRVDVAELYDLAEYRPKVRHVLGKPMFLY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=413, Percent_Identity=25.4237288135593, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI48762920, Length=400, Percent_Identity=25.25, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI266453619, Length=403, Percent_Identity=25.0620347394541, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI266453768, Length=403, Percent_Identity=24.8138957816377, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI266453748, Length=403, Percent_Identity=24.8138957816377, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4505749, Length=403, Percent_Identity=24.8138957816377, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1790350, Length=369, Percent_Identity=30.3523035230352, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17558788, Length=361, Percent_Identity=26.0387811634349, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147584, Length=398, Percent_Identity=23.6180904522613, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321679, Length=285, Percent_Identity=27.3684210526316, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323861, Length=285, Percent_Identity=27.0175438596491, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17647809, Length=407, Percent_Identity=25.0614250614251, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24652337, Length=407, Percent_Identity=25.0614250614251, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573326, Length=408, Percent_Identity=25.2450980392157, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 42449; Mature: 42318

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDRQKTVGVLTGGGDVPGLNPALKTLVTRAVENGYRVLGIRRGWGGLLAYNRDDPHSGE
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCC
STLMPLDRHSVRTVDRSGGTFLHTSRTNPGKVATADVPEFLRGADFDPLNPGHHDFTQHV
CCEEECCCCHHHEEECCCCEEEEECCCCCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHH
LANLEHLGVDSLIAIGGDDTLSYAERLFREGVPVVAIPKTMDNDVFGTDYCIGFSTAVTR
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECHHHHHHH
SVNFIHNLRTSAGSHERIAVVELFGRNSGETSLISAYLAGVDRALISEVPFDVERLALLL
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QEDKRSNRSNYAMVTVSEGASMIGGQTVEYGQEDAYGHKKLGGIGVVVSEAVKGLTGSHI
HHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCHH
IYQQLSYLMRSGAPDSLDLMVAYNYANMTMDLISEGMSGRMVSLQGGTYRHIPISTIMQG
HHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHCC
EKRVDVAELYDLAEYRPKVRHVLGKPMFLY
CCCCCHHHHHHHHHHCHHHHHHHCCCCCCC
>Mature Secondary Structure 
GDRQKTVGVLTGGGDVPGLNPALKTLVTRAVENGYRVLGIRRGWGGLLAYNRDDPHSGE
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCCC
STLMPLDRHSVRTVDRSGGTFLHTSRTNPGKVATADVPEFLRGADFDPLNPGHHDFTQHV
CCEEECCCCHHHEEECCCCEEEEECCCCCCCEEECCHHHHHHCCCCCCCCCCCHHHHHHH
LANLEHLGVDSLIAIGGDDTLSYAERLFREGVPVVAIPKTMDNDVFGTDYCIGFSTAVTR
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECHHHHHHH
SVNFIHNLRTSAGSHERIAVVELFGRNSGETSLISAYLAGVDRALISEVPFDVERLALLL
HHHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
QEDKRSNRSNYAMVTVSEGASMIGGQTVEYGQEDAYGHKKLGGIGVVVSEAVKGLTGSHI
HHHHCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCHH
IYQQLSYLMRSGAPDSLDLMVAYNYANMTMDLISEGMSGRMVSLQGGTYRHIPISTIMQG
HHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCCCEEEECCCEEEECCHHHHHCC
EKRVDVAELYDLAEYRPKVRHVLGKPMFLY
CCCCCHHHHHHHHHHCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]