Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 197119187

GI number: 197119187

Start: 3241200

End: 3241970

Strand: Direct

Name: fabI [H]

Synonym: Gbem_2814

Alternate gene names: 197119187

Gene position: 3241200-3241970 (Clockwise)

Preceding gene: 197119186

Following gene: 197119188

Centisome position: 70.23

GC content: 64.2

Gene sequence:

>771_bases
ATGGGTTTACTTGAAGGTAAAAAGGCACTGATTTTCGGTGTCGCCAACGACAAAAGCATCGCCTGGGCCGTAGCCGAGGC
ATTCAGGCGCGAGGGGGCCGAGATCGCCCTGGCCTACGCCGGAGAAAGCGTGGCCAAAAGGGTAATCCCCTTGGGCGAGA
GCATCGGGGCGAGCATGATCCTTCCCTGCGACGTGAGAAGCGACGAGGACATCGCCCGGCTCTTCTCCGACGTCGCGAAA
CAGTGGGGTGGGCTGGACCTCCTGGTGCACTCGGTAGCGTTCGCCAACAAGGACGAACTCAAGGGGTCGTTTTTGAACAC
CACGCGTGAAGGGTTCGCCACAGCACTCGACATCAGCGCCTATTCCCTCATCGCGCTGGCCAAGGAGGCCGCGCCGCTCA
TGCAGGGGCGCGAGGGAAGCATCATCGCCATGACCTATTACGGCGCGCAGAAGGTTTTCCCCAACTACAACGTGATGGGG
GTTGCCAAGGCCGCGCTTGAGGCAAGCGTGCGTTACCTCGCCGAGGCCATGGGCCCCGAAGGGACCCGCGTCAACGCCAT
CTCCGCAGGCCCCCTGCGCACCCTCGCCTCGGCGGGCATCGGCGGCTTCGGCCAGATTGCAGGCCACGTCGCCGGCAAGG
CGCCGCTTAGGCGCAACATCACCCAGGAGGACGTCGCCAACTCGGCGCTCTACCTCGCCAGCCCGCTCGCCGGCGGGGTG
AGCGGGGAGATCCACTTCGTCGACAGCGGCTACAACATCATCGGCCTGTAA

Upstream 100 bases:

>100_bases
CCTTGCCAACTCATTTTTTATTCATTATTATGTGCCGCTGTGTGTCGTCTCACCGAGTAACGCATCCTAATGCGACGACA
CAGGCAGGGAGATAAGACGC

Downstream 100 bases:

>100_bases
ACGGGAGGCGCGCCATCAAAGGACTGCACGGTAATCTGACAGGGTTGAAGTCGAGCCAGGTAAAGCGGCTCGAAAGGCTG
TACCGCCGCCGTGTGAAACC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MGLLEGKKALIFGVANDKSIAWAVAEAFRREGAEIALAYAGESVAKRVIPLGESIGASMILPCDVRSDEDIARLFSDVAK
QWGGLDLLVHSVAFANKDELKGSFLNTTREGFATALDISAYSLIALAKEAAPLMQGREGSIIAMTYYGAQKVFPNYNVMG
VAKAALEASVRYLAEAMGPEGTRVNAISAGPLRTLASAGIGGFGQIAGHVAGKAPLRRNITQEDVANSALYLASPLAGGV
SGEIHFVDSGYNIIGL

Sequences:

>Translated_256_residues
MGLLEGKKALIFGVANDKSIAWAVAEAFRREGAEIALAYAGESVAKRVIPLGESIGASMILPCDVRSDEDIARLFSDVAK
QWGGLDLLVHSVAFANKDELKGSFLNTTREGFATALDISAYSLIALAKEAAPLMQGREGSIIAMTYYGAQKVFPNYNVMG
VAKAALEASVRYLAEAMGPEGTRVNAISAGPLRTLASAGIGGFGQIAGHVAGKAPLRRNITQEDVANSALYLASPLAGGV
SGEIHFVDSGYNIIGL
>Mature_255_residues
GLLEGKKALIFGVANDKSIAWAVAEAFRREGAEIALAYAGESVAKRVIPLGESIGASMILPCDVRSDEDIARLFSDVAKQ
WGGLDLLVHSVAFANKDELKGSFLNTTREGFATALDISAYSLIALAKEAAPLMQGREGSIIAMTYYGAQKVFPNYNVMGV
AKAALEASVRYLAEAMGPEGTRVNAISAGPLRTLASAGIGGFGQIAGHVAGKAPLRRNITQEDVANSALYLASPLAGGVS
GEIHFVDSGYNIIGL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=244, Percent_Identity=27.8688524590164, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI10190704, Length=263, Percent_Identity=27.3764258555133, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI32483357, Length=249, Percent_Identity=26.1044176706827, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI33667109, Length=195, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=48.4251968503937, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=30.859375, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=26.9076305220884, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367365, Length=252, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=26.0536398467433, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=207, Percent_Identity=28.0193236714976, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=262, Percent_Identity=27.0992366412214, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355319, Length=245, Percent_Identity=26.530612244898, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 26734; Mature: 26603

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLLEGKKALIFGVANDKSIAWAVAEAFRREGAEIALAYAGESVAKRVIPLGESIGASMI
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCEE
LPCDVRSDEDIARLFSDVAKQWGGLDLLVHSVAFANKDELKGSFLNTTREGFATALDISA
EECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHEEEHHHH
YSLIALAKEAAPLMQGREGSIIAMTYYGAQKVFPNYNVMGVAKAALEASVRYLAEAMGPE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTRVNAISAGPLRTLASAGIGGFGQIAGHVAGKAPLRRNITQEDVANSALYLASPLAGGV
CCEEEEECCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHCCCEEEECCCCCCC
SGEIHFVDSGYNIIGL
CCEEEEEECCCEEECC
>Mature Secondary Structure 
GLLEGKKALIFGVANDKSIAWAVAEAFRREGAEIALAYAGESVAKRVIPLGESIGASMI
CCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCEE
LPCDVRSDEDIARLFSDVAKQWGGLDLLVHSVAFANKDELKGSFLNTTREGFATALDISA
EECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHEEEHHHH
YSLIALAKEAAPLMQGREGSIIAMTYYGAQKVFPNYNVMGVAKAALEASVRYLAEAMGPE
HHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GTRVNAISAGPLRTLASAGIGGFGQIAGHVAGKAPLRRNITQEDVANSALYLASPLAGGV
CCEEEEECCCHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCHHHHHCCCEEEECCCCCCC
SGEIHFVDSGYNIIGL
CCEEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]