Click here to switch to the map view.

The map label for this gene is pth

Identifier: 197119139

GI number: 197119139

Start: 3194190

End: 3194756

Strand: Reverse

Name: pth

Synonym: Gbem_2766

Alternate gene names: 197119139

Gene position: 3194756-3194190 (Counterclockwise)

Preceding gene: 197119140

Following gene: 197119138

Centisome position: 69.22

GC content: 61.2

Gene sequence:

>567_bases
ATGGCAGCAAAACTTATCGTAGGGCTCGGCAACCCCGGGCCAAAGTACTCATGGACCCGCCACAACGCGGGTTTCATGGT
TTTGGACCGCTTAGCCAGCCTCTCGGGAATCCAGGTCACCAGGAAGGCCTTCTCCGGCCTTTCTGGTGACGGAAACTGGT
CCTCCGAGCGGGTCTACCTCCTCAAGCCGCAGACCTTCATGAACCTGTCGGGGCGCTCCGTCGCGGAGGCGCTTCGCTTC
TACAAACTGTCCCTCTCAGATCTCATCGTGATCCATGACGACCTGGATATCCCGTTCGGCAAGGTAAAGCTCAAGGAGGG
AGGCGGCCACGGCGGGCACAACGGCCTGCGCTCGCTGGCCCAGGAGCTCGGCTCTTCCGCCTATGCGCGCATCCGCGTCG
GCATCGGCCGCCCCGTGCACGGCGACGTGGTGAACTTCGTGCTCACGAACTTCGCCAAGGAAGAGATGGATTCTCTCCTC
GAGGTGCTGGACACCTCGGTCGACGCGCTGGAGATGATGATCAAGGAAGGGATGCCCAAGGCAATGAGCATCTTCAACGC
CAGGTAG

Upstream 100 bases:

>100_bases
CGCAATCCTCTCCGACCCCAAAGCGTCCGTGGTCAGCATCCTCGGCCGCAAGGGTGCCGCAGAGGAAGAGGCCGCTCCCG
CCGCCTAATCCAGGAATCGT

Downstream 100 bases:

>100_bases
CAGATCCCCACTTCACGAAGGTGGACTTTATAGCACTCCCCCCGGCAATGGGTTGAGTTTTCAAAAGGATACGTCATGGG
TTTCAATTGCGGTATCGTCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MAAKLIVGLGNPGPKYSWTRHNAGFMVLDRLASLSGIQVTRKAFSGLSGDGNWSSERVYLLKPQTFMNLSGRSVAEALRF
YKLSLSDLIVIHDDLDIPFGKVKLKEGGGHGGHNGLRSLAQELGSSAYARIRVGIGRPVHGDVVNFVLTNFAKEEMDSLL
EVLDTSVDALEMMIKEGMPKAMSIFNAR

Sequences:

>Translated_188_residues
MAAKLIVGLGNPGPKYSWTRHNAGFMVLDRLASLSGIQVTRKAFSGLSGDGNWSSERVYLLKPQTFMNLSGRSVAEALRF
YKLSLSDLIVIHDDLDIPFGKVKLKEGGGHGGHNGLRSLAQELGSSAYARIRVGIGRPVHGDVVNFVLTNFAKEEMDSLL
EVLDTSVDALEMMIKEGMPKAMSIFNAR
>Mature_187_residues
AAKLIVGLGNPGPKYSWTRHNAGFMVLDRLASLSGIQVTRKAFSGLSGDGNWSSERVYLLKPQTFMNLSGRSVAEALRFY
KLSLSDLIVIHDDLDIPFGKVKLKEGGGHGGHNGLRSLAQELGSSAYARIRVGIGRPVHGDVVNFVLTNFAKEEMDSLLE
VLDTSVDALEMMIKEGMPKAMSIFNAR

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=176, Percent_Identity=38.0681818181818, Blast_Score=114, Evalue=7e-26,
Organism=Escherichia coli, GI1787455, Length=192, Percent_Identity=42.1875, Blast_Score=112, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321983, Length=169, Percent_Identity=32.5443786982249, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_GEOBB (B5EHX1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139566.1
- GeneID:   6782764
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2766
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20465; Mature: 20334

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKLIVGLGNPGPKYSWTRHNAGFMVLDRLASLSGIQVTRKAFSGLSGDGNWSSERVYL
CCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEE
LKPQTFMNLSGRSVAEALRFYKLSLSDLIVIHDDLDIPFGKVKLKEGGGHGGHNGLRSLA
ECCHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHH
QELGSSAYARIRVGIGRPVHGDVVNFVLTNFAKEEMDSLLEVLDTSVDALEMMIKEGMPK
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCH
AMSIFNAR
HHHHHCCC
>Mature Secondary Structure 
AAKLIVGLGNPGPKYSWTRHNAGFMVLDRLASLSGIQVTRKAFSGLSGDGNWSSERVYL
CCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEE
LKPQTFMNLSGRSVAEALRFYKLSLSDLIVIHDDLDIPFGKVKLKEGGGHGGHNGLRSLA
ECCHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHH
QELGSSAYARIRVGIGRPVHGDVVNFVLTNFAKEEMDSLLEVLDTSVDALEMMIKEGMPK
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCH
AMSIFNAR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA