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The map label for this gene is appA [H]

Identifier: 197119021

GI number: 197119021

Start: 3060436

End: 3062037

Strand: Reverse

Name: appA [H]

Synonym: Gbem_2644

Alternate gene names: 197119021

Gene position: 3062037-3060436 (Counterclockwise)

Preceding gene: 197119022

Following gene: 197119020

Centisome position: 66.35

GC content: 62.55

Gene sequence:

>1602_bases
ATGACCTTTTCCCGCTGTCTCCCCCTCCTTCTAGCCCTCCTTGTCTTCGCCGGTTGCGCCAAGGGTGCGCCTGAGGACAA
GGCGCGCGCGCAGGGCGGGGCGGCTTCCCGCGGCAGCTCCTTTGTTACCGGCAGCATCGGCGAGCCCTCGAACCTGATAC
CGATACTGTCCGCCGACTCCGCCTCCAGCGATGTGGCTGGCCTGGTCTACAACGGGCTGGTGCGCTACGACAAGAACCTG
AAGCTGGAGGGGGACCTGGCCCAGTCGTGGGAGGTTTCCCCCGACGGCCTAGGCATCACCTTCCACCTGCGCCGCGGCGT
CAAGTGGCACGACGGCCACGACTTCACCTCCCGCGACGTCCTCTACACCTACAAGGTCACCATCGACCCCAAGACGCCGA
CCGCGTATGCGGAGGACTTCAAGCAGGTGCAGACCGCCGAGGCGGTGGATACTTACACCTTCAAGGTGCGTTACGCCAAG
CCCTTCGCACCGGCCTTGGCCTCGTGGGGGATGCCGGTGCTGCCGGCTCACCTTCTGGAGGGGAAGGACATCACCAAGAG
CCCGCTCTCCAGGAAGCCGGTCGGTACCGGACCCTACATCTTCAAGGAATGGGTGCCGGGACAGAGGTTGATCCTGGAGG
CGAACCCGCATTACTACGAGGGGGCGCCGCACCTCTCGCCCTACGTCTATCGCATCATCCCGGACAACTCCACCATGTAC
ATGGAGCTCAAGGCTGGCGGCATCGACATGATGGGGCTTTCCCCGGTGCAGTACCAGCGCCAGACCGGCAGCCGCGAGTT
CCTGTCCCGTTTCAACAAGTACCGCTATCCGGCCTCCGCTTACACCTACCTGGGGTACAACCTGCGGCTTCCCATGTTCC
AGGACGTCAGGGTGCGCCGCGCGCTCACCTGCGCCATCAACAAAGAAGAGATCATCCAGGGGGTCCTGCTCGGGATGGGG
CAGATAGCGCATGGTCCGTACAAGCCGGGGACCTGGGCCTGGAAACCGAAGATCGAAGCCGACCCCGGCTACGATCCGGC
TCGCGCCGCGGCACTCTTGAAGGAAGCAGGGTACGTCATGGGGAGTGACGGCATCCTGGTCAAGGACGGCAAGCCGTTGA
GCTTCACCATCATGACCAACCAGGGGAACGATCAGAGGCTCAAATGCGCCCAGATCATTCAGCGGCGCCTGAAGCGGGTC
GGCATAGACGTCAAGATCCGCGTCATGGAATGGGCCTCCTTCCTCACCAACTTCATCGACAAGGGTAGGTTCGAGGCGGT
GCTGCTCGGTTGGACCATCTCCCAGGACCCCGACCTTTACGACGTCTGGCACTCGTCCAAGACCGGCCCCAAGGAGCTGA
ACTTCATCGGCTACAAGAACCCGGATTTGGACCGGCTGATCGTGGAGGGGCGCGGCACCTTCGATATGGGGAAACGGCGC
GAGAGCTACTACCGGATCCAGGAGATACTGGCACAGGACCAGCCTTACACCTTCCTGTACGTCCCGGACGCGCTCCCGGT
AGTCGCCTCCAGGATCAAGGGGATTGAGCCTGCACCTGCGGGGATCAGCTATAACTTGATCAAATGGTACGTGGAACAGT
GA

Upstream 100 bases:

>100_bases
AGGCGCTCCCGCTGTACCGGAAGCTGCAGAAGCTGGAGCCCGAGCAGAACGCCGAGCTCGCCGAGAAGATCAGGCACTGC
CGCCAGGAGAGCGGGGAAAA

Downstream 100 bases:

>100_bases
ACCAATTGACGATTGACAATTGACAATTGACAACGAAAAACTCAACAACTATCTATCGCCACTCATCTGTTTATGGCGCA
CTTCCCGCTCTGGAAAGAGA

Product: peptide ABC transporter periplasmic peptide-binding lipoprotein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MTFSRCLPLLLALLVFAGCAKGAPEDKARAQGGAASRGSSFVTGSIGEPSNLIPILSADSASSDVAGLVYNGLVRYDKNL
KLEGDLAQSWEVSPDGLGITFHLRRGVKWHDGHDFTSRDVLYTYKVTIDPKTPTAYAEDFKQVQTAEAVDTYTFKVRYAK
PFAPALASWGMPVLPAHLLEGKDITKSPLSRKPVGTGPYIFKEWVPGQRLILEANPHYYEGAPHLSPYVYRIIPDNSTMY
MELKAGGIDMMGLSPVQYQRQTGSREFLSRFNKYRYPASAYTYLGYNLRLPMFQDVRVRRALTCAINKEEIIQGVLLGMG
QIAHGPYKPGTWAWKPKIEADPGYDPARAAALLKEAGYVMGSDGILVKDGKPLSFTIMTNQGNDQRLKCAQIIQRRLKRV
GIDVKIRVMEWASFLTNFIDKGRFEAVLLGWTISQDPDLYDVWHSSKTGPKELNFIGYKNPDLDRLIVEGRGTFDMGKRR
ESYYRIQEILAQDQPYTFLYVPDALPVVASRIKGIEPAPAGISYNLIKWYVEQ

Sequences:

>Translated_533_residues
MTFSRCLPLLLALLVFAGCAKGAPEDKARAQGGAASRGSSFVTGSIGEPSNLIPILSADSASSDVAGLVYNGLVRYDKNL
KLEGDLAQSWEVSPDGLGITFHLRRGVKWHDGHDFTSRDVLYTYKVTIDPKTPTAYAEDFKQVQTAEAVDTYTFKVRYAK
PFAPALASWGMPVLPAHLLEGKDITKSPLSRKPVGTGPYIFKEWVPGQRLILEANPHYYEGAPHLSPYVYRIIPDNSTMY
MELKAGGIDMMGLSPVQYQRQTGSREFLSRFNKYRYPASAYTYLGYNLRLPMFQDVRVRRALTCAINKEEIIQGVLLGMG
QIAHGPYKPGTWAWKPKIEADPGYDPARAAALLKEAGYVMGSDGILVKDGKPLSFTIMTNQGNDQRLKCAQIIQRRLKRV
GIDVKIRVMEWASFLTNFIDKGRFEAVLLGWTISQDPDLYDVWHSSKTGPKELNFIGYKNPDLDRLIVEGRGTFDMGKRR
ESYYRIQEILAQDQPYTFLYVPDALPVVASRIKGIEPAPAGISYNLIKWYVEQ
>Mature_532_residues
TFSRCLPLLLALLVFAGCAKGAPEDKARAQGGAASRGSSFVTGSIGEPSNLIPILSADSASSDVAGLVYNGLVRYDKNLK
LEGDLAQSWEVSPDGLGITFHLRRGVKWHDGHDFTSRDVLYTYKVTIDPKTPTAYAEDFKQVQTAEAVDTYTFKVRYAKP
FAPALASWGMPVLPAHLLEGKDITKSPLSRKPVGTGPYIFKEWVPGQRLILEANPHYYEGAPHLSPYVYRIIPDNSTMYM
ELKAGGIDMMGLSPVQYQRQTGSREFLSRFNKYRYPASAYTYLGYNLRLPMFQDVRVRRALTCAINKEEIIQGVLLGMGQ
IAHGPYKPGTWAWKPKIEADPGYDPARAAALLKEAGYVMGSDGILVKDGKPLSFTIMTNQGNDQRLKCAQIIQRRLKRVG
IDVKIRVMEWASFLTNFIDKGRFEAVLLGWTISQDPDLYDVWHSSKTGPKELNFIGYKNPDLDRLIVEGRGTFDMGKRRE
SYYRIQEILAQDQPYTFLYVPDALPVVASRIKGIEPAPAGISYNLIKWYVEQ

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=518, Percent_Identity=28.1853281853282, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1787762, Length=523, Percent_Identity=26.7686424474187, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787052, Length=535, Percent_Identity=27.2897196261682, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1787551, Length=543, Percent_Identity=25.9668508287293, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789887, Length=450, Percent_Identity=26.2222222222222, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789397, Length=498, Percent_Identity=24.6987951807229, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1787495, Length=527, Percent_Identity=24.0986717267552, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87081878, Length=534, Percent_Identity=24.1573033707865, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI87082063, Length=432, Percent_Identity=22.2222222222222, Blast_Score=89, Evalue=8e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59637; Mature: 59506

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSRCLPLLLALLVFAGCAKGAPEDKARAQGGAASRGSSFVTGSIGEPSNLIPILSADS
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCC
ASSDVAGLVYNGLVRYDKNLKLEGDLAQSWEVSPDGLGITFHLRRGVKWHDGHDFTSRDV
CCCCHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCE
LYTYKVTIDPKTPTAYAEDFKQVQTAEAVDTYTFKVRYAKPFAPALASWGMPVLPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHCCCCCCHHHHHC
GKDITKSPLSRKPVGTGPYIFKEWVPGQRLILEANPHYYEGAPHLSPYVYRIIPDNSTMY
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEECCCCCCCCEEEEEEECCCEEE
MELKAGGIDMMGLSPVQYQRQTGSREFLSRFNKYRYPASAYTYLGYNLRLPMFQDVRVRR
EEEECCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHH
ALTCAINKEEIIQGVLLGMGQIAHGPYKPGTWAWKPKIEADPGYDPARAAALLKEAGYVM
HHHEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCEEE
GSDGILVKDGKPLSFTIMTNQGNDQRLKCAQIIQRRLKRVGIDVKIRVMEWASFLTNFID
CCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHC
KGRFEAVLLGWTISQDPDLYDVWHSSKTGPKELNFIGYKNPDLDRLIVEGRGTFDMGKRR
CCCEEEEEEEEEECCCCCHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHH
ESYYRIQEILAQDQPYTFLYVPDALPVVASRIKGIEPAPAGISYNLIKWYVEQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
TFSRCLPLLLALLVFAGCAKGAPEDKARAQGGAASRGSSFVTGSIGEPSNLIPILSADS
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCC
ASSDVAGLVYNGLVRYDKNLKLEGDLAQSWEVSPDGLGITFHLRRGVKWHDGHDFTSRDV
CCCCHHHHHHHHHHEECCCCEEECCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCE
LYTYKVTIDPKTPTAYAEDFKQVQTAEAVDTYTFKVRYAKPFAPALASWGMPVLPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHCCCCCCHHHHHC
GKDITKSPLSRKPVGTGPYIFKEWVPGQRLILEANPHYYEGAPHLSPYVYRIIPDNSTMY
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEECCCCEECCCCCCCCEEEEEEECCCEEE
MELKAGGIDMMGLSPVQYQRQTGSREFLSRFNKYRYPASAYTYLGYNLRLPMFQDVRVRR
EEEECCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHHHHHH
ALTCAINKEEIIQGVLLGMGQIAHGPYKPGTWAWKPKIEADPGYDPARAAALLKEAGYVM
HHHEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCEEE
GSDGILVKDGKPLSFTIMTNQGNDQRLKCAQIIQRRLKRVGIDVKIRVMEWASFLTNFID
CCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHC
KGRFEAVLLGWTISQDPDLYDVWHSSKTGPKELNFIGYKNPDLDRLIVEGRGTFDMGKRR
CCCEEEEEEEEEECCCCCHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHH
ESYYRIQEILAQDQPYTFLYVPDALPVVASRIKGIEPAPAGISYNLIKWYVEQ
HHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]