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The map label for this gene is appB [H]

Identifier: 197119020

GI number: 197119020

Start: 3059263

End: 3060243

Strand: Reverse

Name: appB [H]

Synonym: Gbem_2643

Alternate gene names: 197119020

Gene position: 3060243-3059263 (Counterclockwise)

Preceding gene: 197119021

Following gene: 197119019

Centisome position: 66.31

GC content: 63.4

Gene sequence:

>981_bases
ATGACCAACTACCTGATAAAACGCATACTCATGCTCTTCCCGCTGCTGCTCGGGATCACGCTGATCACCTTTACGGTGAT
TCATCTGGCACCGGGGGAACCGGTCGAGATGCAGACGGCGATGAGCCCGAAGGTCTCGGCTCAGGCTAGGGCGAGGCTGC
GCGAGTTCTACGGCCTCGACAAGCCGCTCCACGTGCAGTACGGCATGTGGCTTTCCAACCTGGCGCACCTGGACTTCGGA
CGCTCGTTCGCGCCGGACAACCGCCCGGTGCTGGACAAGATCAAGGAACGGATCCCCATTACCATCTCGCTGAACGTGAT
CGCGCTGATCCTGGAGTTCGGGCTCGCCCTCCCCATCGGTATCCTCGCGGCGGTGCACCGGGACACGCTTCTCGACCGCG
CCATCTCGGTCTTCGTCTTTGCCGGGTTCGCCGTTCCGACCTTCTGGCTTGCGCTGCTCTGCATGTACTTCTTCGGCGTG
AAGCTGAACTGGCTCCCCATCTCGGGCATCCATTCGCTCGGCAGCGACGCGCTTCCCACGCTGGCCCGTTTCTGGGACCT
GGCGAAGCACCTTCTGCTTCCCATCTCCATCGCCACCTTCGGAAGCCTCGCCGGCCTTTCCCGCTACATGCGCTCCACCA
TGCTCGAAGTGCTCTCGCAGGATTACATCACCACCGCCCGGGCCAAGGGGGTGAAGGAGCGGGTGGTGATCTACCGGCAC
GCCCTGAAAAACGCGCTGCTTCCCGTGATCACGCTCCTCGGCTTCTCGCTTCCCGCCCTGATCGGCGGCAGCGTCATCTT
CGAGACCATATTCTCCATCCCCGGGATGGGGCAGCTCTTCTACCAGGGGGTCATGGCGCGCGACTACCCGCTGGTCATGG
GGATCCTCGTGATCGGCGCCGGGCTCACGCTCGTCGGCAACCTTTTGGCCGACCTCTGCTATGCCTTCGCGGATCCCCGC
ATCCGGCACGGGAGGGGCTAG

Upstream 100 bases:

>100_bases
GAAAGAGAGTGGGGATCCTGAATGATCTGTATCGACGAATAGGTTGAAGTGTTAAAAAGCTTCTCCTTTATCTTTATCTT
TATCTTAATCTGGTTCATCA

Downstream 100 bases:

>100_bases
CCCATGAACGGACCTTTCCGCGAGTTCTGGGAACGCTTCAGCGCCAACCGCTTCGCCCTGGCTGGCCTTTGGGTGATTGC
CCTTTTGTTCCTCCTTTCCT

Product: peptide ABC transporter membrane protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTNYLIKRILMLFPLLLGITLITFTVIHLAPGEPVEMQTAMSPKVSAQARARLREFYGLDKPLHVQYGMWLSNLAHLDFG
RSFAPDNRPVLDKIKERIPITISLNVIALILEFGLALPIGILAAVHRDTLLDRAISVFVFAGFAVPTFWLALLCMYFFGV
KLNWLPISGIHSLGSDALPTLARFWDLAKHLLLPISIATFGSLAGLSRYMRSTMLEVLSQDYITTARAKGVKERVVIYRH
ALKNALLPVITLLGFSLPALIGGSVIFETIFSIPGMGQLFYQGVMARDYPLVMGILVIGAGLTLVGNLLADLCYAFADPR
IRHGRG

Sequences:

>Translated_326_residues
MTNYLIKRILMLFPLLLGITLITFTVIHLAPGEPVEMQTAMSPKVSAQARARLREFYGLDKPLHVQYGMWLSNLAHLDFG
RSFAPDNRPVLDKIKERIPITISLNVIALILEFGLALPIGILAAVHRDTLLDRAISVFVFAGFAVPTFWLALLCMYFFGV
KLNWLPISGIHSLGSDALPTLARFWDLAKHLLLPISIATFGSLAGLSRYMRSTMLEVLSQDYITTARAKGVKERVVIYRH
ALKNALLPVITLLGFSLPALIGGSVIFETIFSIPGMGQLFYQGVMARDYPLVMGILVIGAGLTLVGNLLADLCYAFADPR
IRHGRG
>Mature_325_residues
TNYLIKRILMLFPLLLGITLITFTVIHLAPGEPVEMQTAMSPKVSAQARARLREFYGLDKPLHVQYGMWLSNLAHLDFGR
SFAPDNRPVLDKIKERIPITISLNVIALILEFGLALPIGILAAVHRDTLLDRAISVFVFAGFAVPTFWLALLCMYFFGVK
LNWLPISGIHSLGSDALPTLARFWDLAKHLLLPISIATFGSLAGLSRYMRSTMLEVLSQDYITTARAKGVKERVVIYRHA
LKNALLPVITLLGFSLPALIGGSVIFETIFSIPGMGQLFYQGVMARDYPLVMGILVIGAGLTLVGNLLADLCYAFADPRI
RHGRG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=327, Percent_Identity=38.5321100917431, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=35.7771260997067, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1787053, Length=323, Percent_Identity=34.6749226006192, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1787497, Length=325, Percent_Identity=34.4615384615385, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI1788504, Length=371, Percent_Identity=30.7277628032345, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787761, Length=336, Percent_Identity=36.6071428571429, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787550, Length=270, Percent_Identity=29.2592592592593, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35992; Mature: 35860

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYLIKRILMLFPLLLGITLITFTVIHLAPGEPVEMQTAMSPKVSAQARARLREFYGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCC
KPLHVQYGMWLSNLAHLDFGRSFAPDNRPVLDKIKERIPITISLNVIALILEFGLALPIG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
ILAAVHRDTLLDRAISVFVFAGFAVPTFWLALLCMYFFGVKLNWLPISGIHSLGSDALPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCHHHHH
LARFWDLAKHLLLPISIATFGSLAGLSRYMRSTMLEVLSQDYITTARAKGVKERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALKNALLPVITLLGFSLPALIGGSVIFETIFSIPGMGQLFYQGVMARDYPLVMGILVIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GLTLVGNLLADLCYAFADPRIRHGRG
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TNYLIKRILMLFPLLLGITLITFTVIHLAPGEPVEMQTAMSPKVSAQARARLREFYGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCC
KPLHVQYGMWLSNLAHLDFGRSFAPDNRPVLDKIKERIPITISLNVIALILEFGLALPIG
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHH
ILAAVHRDTLLDRAISVFVFAGFAVPTFWLALLCMYFFGVKLNWLPISGIHSLGSDALPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHCCCHHHHH
LARFWDLAKHLLLPISIATFGSLAGLSRYMRSTMLEVLSQDYITTARAKGVKERVVIYRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ALKNALLPVITLLGFSLPALIGGSVIFETIFSIPGMGQLFYQGVMARDYPLVMGILVIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHH
GLTLVGNLLADLCYAFADPRIRHGRG
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]