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The map label for this gene is tilS [H]

Identifier: 197119017

GI number: 197119017

Start: 3056034

End: 3057407

Strand: Reverse

Name: tilS [H]

Synonym: Gbem_2640

Alternate gene names: 197119017

Gene position: 3057407-3056034 (Counterclockwise)

Preceding gene: 197119018

Following gene: 197119016

Centisome position: 66.25

GC content: 65.07

Gene sequence:

>1374_bases
TTGAAGACCTTAGTTACCAAAGTACGTAACCAGAACCTCTTTCAGCCGGGCGAGACCGTGGTGGTGGCGGTGTCCGGAGG
GGCCGATTCTGTAGCACTTCTCGACATCATGACGCGGCTGGAGTGCGGGCTGCGCCTGGTGGTCGCCCACCTGAACCACT
GCCTGAGGGGCGCCGAATCGGATGCCGACCAGGCCTTTGTCGCCTCCCTCGCCGACAACTATCACCTTCCCTTCGTTTCC
CAAAGCTGCGACGTCGCAGATCTGGCGCGCCGCGAGCGGCTCTCCCTGGAGGACGCCGGTCGCCGGGCGCGCTACGCCTT
CCTGGAGCAGACTGCCGAGCAGTACGGGGCCTTCAGCATCGCGCTGGCGCACCACCAGGACGACCAGGCCGAGACCGTGC
TGATCCGCCTCTTGCGCGGTTCCGGCGGGACAGGCCTCTCCGCCATGGCCGGTACGGCCGGCGATAAGCTGAAACGCCCG
CTCTTGCAGGTCTCCCGCGTAAAGCTGGAGCAGTACTTGAAACAGCGGGAGCTAAGCTATCGGACCGACTCGACCAACGC
CGACACCGCCATCCTTCGTAACAGCATCCGTCACGAGCTGATCCCGTTATTGAGGCGCTACAACCCCAAGGTCTCGGAGC
GTCTGGCAGCCACCGCGGAGATACTTGCCGGCGACGAGGAACTGCTGGAAGGCCTCACTCAAGCCGCCTACCAAAGGCTC
GCCTCAGCCGAGGGGGGGCAGGTCCGCTTTCAGGTCGAGCCGCTCATGAAGGAGCCGCGCGCCCTTCGTCTGCGCCTTTA
CCGGCGCGCGCTCACGGAGCTGCGCGGCGACCTGATGCGGATCGGCCTTGCGCACCTGGAGGCTATCGACCTCCTTGCCG
CGTCCCCCCGCCCCAACGCAGAGGCAACGCTCCCAGGAGCATGCCGGGTTTCGCGCTGCTACGGCTCACTTTCCCTCGGC
CACGCCGCCGCTCAGGCACCACCCCTATGGGAGCAGGTGGTTGCGGGGGAGGGGCGCTACCCGCTTCCTGGCGGAGGGGT
GCTGCTGGTAGAGCGCCTGCCGCGTCCGGATAGCCTCGACACCGGGTCGCGCTGGAGCGCCTTCCTCTCTCCCGAAACTG
TCCCTTTTCCTTGGCTTTTGCGCGGCTTCCGGCCCGGCGACCGTTTCACGCCGCTGGGCATGAGCGGGGCGCAGAAAATC
AAGGACGCTTTCATAAATGAAAAGGTGCCGCCGCAGTTGCGCAGACGCATACCGCTTTTGCTGAGCGAGGGGGAAATTGC
CTGGGTCCCGGGGGTGAGACTTGCGGAACGGGGTCGGGTGACACCCCGGATTGACGCGGTCTTACGGGTGGAAATTCTTG
AAATTACACCTTAA

Upstream 100 bases:

>100_bases
TCTAAAGACTTTGACTTTCCCCCCTAAAAATCTCTGTGACCTCCGTGGACCTCAGCGACCTCTGTGTTCCGCCTTTTCGG
GGTGAACCTTTAGGAGTCTA

Downstream 100 bases:

>100_bases
AGCTCTTGTCAACCAGCACCCTTTATGGTAATCCTTTTGTTCATTTGAACGCATATTTTTCTCTCTCATAAGTGGCCGTA
AAATCGACTCTTAGACAAAC

Product: tRNA(Ile) lysidine-34 synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKTLVTKVRNQNLFQPGETVVVAVSGGADSVALLDIMTRLECGLRLVVAHLNHCLRGAESDADQAFVASLADNYHLPFVS
QSCDVADLARRERLSLEDAGRRARYAFLEQTAEQYGAFSIALAHHQDDQAETVLIRLLRGSGGTGLSAMAGTAGDKLKRP
LLQVSRVKLEQYLKQRELSYRTDSTNADTAILRNSIRHELIPLLRRYNPKVSERLAATAEILAGDEELLEGLTQAAYQRL
ASAEGGQVRFQVEPLMKEPRALRLRLYRRALTELRGDLMRIGLAHLEAIDLLAASPRPNAEATLPGACRVSRCYGSLSLG
HAAAQAPPLWEQVVAGEGRYPLPGGGVLLVERLPRPDSLDTGSRWSAFLSPETVPFPWLLRGFRPGDRFTPLGMSGAQKI
KDAFINEKVPPQLRRRIPLLLSEGEIAWVPGVRLAERGRVTPRIDAVLRVEILEITP

Sequences:

>Translated_457_residues
MKTLVTKVRNQNLFQPGETVVVAVSGGADSVALLDIMTRLECGLRLVVAHLNHCLRGAESDADQAFVASLADNYHLPFVS
QSCDVADLARRERLSLEDAGRRARYAFLEQTAEQYGAFSIALAHHQDDQAETVLIRLLRGSGGTGLSAMAGTAGDKLKRP
LLQVSRVKLEQYLKQRELSYRTDSTNADTAILRNSIRHELIPLLRRYNPKVSERLAATAEILAGDEELLEGLTQAAYQRL
ASAEGGQVRFQVEPLMKEPRALRLRLYRRALTELRGDLMRIGLAHLEAIDLLAASPRPNAEATLPGACRVSRCYGSLSLG
HAAAQAPPLWEQVVAGEGRYPLPGGGVLLVERLPRPDSLDTGSRWSAFLSPETVPFPWLLRGFRPGDRFTPLGMSGAQKI
KDAFINEKVPPQLRRRIPLLLSEGEIAWVPGVRLAERGRVTPRIDAVLRVEILEITP
>Mature_457_residues
MKTLVTKVRNQNLFQPGETVVVAVSGGADSVALLDIMTRLECGLRLVVAHLNHCLRGAESDADQAFVASLADNYHLPFVS
QSCDVADLARRERLSLEDAGRRARYAFLEQTAEQYGAFSIALAHHQDDQAETVLIRLLRGSGGTGLSAMAGTAGDKLKRP
LLQVSRVKLEQYLKQRELSYRTDSTNADTAILRNSIRHELIPLLRRYNPKVSERLAATAEILAGDEELLEGLTQAAYQRL
ASAEGGQVRFQVEPLMKEPRALRLRLYRRALTELRGDLMRIGLAHLEAIDLLAASPRPNAEATLPGACRVSRCYGSLSLG
HAAAQAPPLWEQVVAGEGRYPLPGGGVLLVERLPRPDSLDTGSRWSAFLSPETVPFPWLLRGFRPGDRFTPLGMSGAQKI
KDAFINEKVPPQLRRRIPLLLSEGEIAWVPGVRLAERGRVTPRIDAVLRVEILEITP

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=438, Percent_Identity=29.2237442922374, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50299; Mature: 50299

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLVTKVRNQNLFQPGETVVVAVSGGADSVALLDIMTRLECGLRLVVAHLNHCLRGAES
CCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DADQAFVASLADNYHLPFVSQSCDVADLARRERLSLEDAGRRARYAFLEQTAEQYGAFSI
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHCCEEE
ALAHHQDDQAETVLIRLLRGSGGTGLSAMAGTAGDKLKRPLLQVSRVKLEQYLKQRELSY
EEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RTDSTNADTAILRNSIRHELIPLLRRYNPKVSERLAATAEILAGDEELLEGLTQAAYQRL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASAEGGQVRFQVEPLMKEPRALRLRLYRRALTELRGDLMRIGLAHLEAIDLLAASPRPNA
HCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EATLPGACRVSRCYGSLSLGHAAAQAPPLWEQVVAGEGRYPLPGGGVLLVERLPRPDSLD
CCCCCCHHHHHHHHCCHHHCHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
TGSRWSAFLSPETVPFPWLLRGFRPGDRFTPLGMSGAQKIKDAFINEKVPPQLRRRIPLL
CCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCEE
LSEGEIAWVPGVRLAERGRVTPRIDAVLRVEILEITP
EECCCEEECCCCHHHHCCCCCHHHHHEEEEEEEEECC
>Mature Secondary Structure
MKTLVTKVRNQNLFQPGETVVVAVSGGADSVALLDIMTRLECGLRLVVAHLNHCLRGAES
CCHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DADQAFVASLADNYHLPFVSQSCDVADLARRERLSLEDAGRRARYAFLEQTAEQYGAFSI
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHCCEEE
ALAHHQDDQAETVLIRLLRGSGGTGLSAMAGTAGDKLKRPLLQVSRVKLEQYLKQRELSY
EEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RTDSTNADTAILRNSIRHELIPLLRRYNPKVSERLAATAEILAGDEELLEGLTQAAYQRL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
ASAEGGQVRFQVEPLMKEPRALRLRLYRRALTELRGDLMRIGLAHLEAIDLLAASPRPNA
HCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EATLPGACRVSRCYGSLSLGHAAAQAPPLWEQVVAGEGRYPLPGGGVLLVERLPRPDSLD
CCCCCCHHHHHHHHCCHHHCHHHHCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
TGSRWSAFLSPETVPFPWLLRGFRPGDRFTPLGMSGAQKIKDAFINEKVPPQLRRRIPLL
CCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCEE
LSEGEIAWVPGVRLAERGRVTPRIDAVLRVEILEITP
EECCCEEECCCCHHHHCCCCCHHHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA