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The map label for this gene is fixL [H]

Identifier: 197118914

GI number: 197118914

Start: 2938790

End: 2940817

Strand: Reverse

Name: fixL [H]

Synonym: Gbem_2537

Alternate gene names: 197118914

Gene position: 2940817-2938790 (Counterclockwise)

Preceding gene: 197118916

Following gene: 197118913

Centisome position: 63.72

GC content: 54.88

Gene sequence:

>2028_bases
ATGGAAAAACTCACAATAACAAGCAGACTTTTGCGCATGGTTACCAGCGCACTGCTGGTTTTCTTGGCCACGGTAGTGGG
AGTGAAGCTGCTGCAGCCCTTGGGCCTACGTCACCCTTTACTGATTTATTATCCTGCAGTTGCCGTGTCGGCGATTTACC
TCGGGCTGGAAGGGGGACTGACGGCGACGCTTCTGTCGGCACTTTCGGCGGCATACTTCTCAATCGAGCCGCGCTCGAAT
TTTGCTATACGAGACTTCGAGGAGCAACTCTACGTCGCCGTCTTCGTGCTGGAAGGGTGCATCATCTCGTATGCCGCCTA
CAAGATGCGTCGCGCTGAAACCGCAGAAGCGCAGATAAAAATCGCCAAGGAAAGAACGCTGGCGGCTGAAAGACTGGAGG
AAAGCGAGATACGTCTGCGCCTGGCACTGGAGGGCGCCAAGATGGGTATGTGGACTTTGGATGTGACGTCGAATCAATGC
GAGTTGAGCGACCAGGAATACGATCTGCTGGGCGACACCAAGGGAGGAGGCCTGGAGCCGGTTGATGCTTTCTTTGAGAG
GGTACATCCCGATGACCGCGAGGAATTCAAACGCCTCCTTAGTGAGGTGCTGGATCACGGAGACAATTTTTCCCATGAAT
TCCGCATCGTCCGTCCGGATGGCGAAATCCGTTGGCTCGCCGCACGAGCGCGGTTATCACGCAGTCGAAAGGGAAAGCCA
ATTCGGATGCATGGGGTCAACTATGACGTCACTTCCCAAAGAAGGTTTGAGGAGCAACTCCGACACAGCGAGAACGAAGC
GCGTGCTCGAGCAAACGAGTTAGCAGTATTAATGGATACCGTGCCAGCCATCACCTTCATAGCACACGACCCCGAATGCC
GGGATATGTCGGGAAGTCGCAAGTCTCGTGAAATGCTACGCGTACCAGAGGGGAAAAGCGTATCGGCGTCTGCCCCCGAG
GGCGAGTTCGCAAACAGTTTCCATGGGTTCAAGGACGGCAGAGAGCTCTCCCCGGAGGAACTTCCCGTCCAGATGGCTGC
GAGGGGGCAGGAAGTACATGATTACGAGCTTACCCTTGTCTTCAACGACGGAACATCTTGTGAGGTTATTGGCAATGCAG
TGCCGCTTTTCGATGGCGCAGGCAAGGCCCGTGGCGCAATCGGGGCTTTCCTTGACATCACCGAGCAGAAAAGGATCCAG
CGGGAACTGGAAAAGGTCCGCGACGAACTGGATAAACGGGTGAAGGAACGGACGGCCGAGTTGCGCCAAGCCCTTGAGAC
CTTACAAAGGGAGACCGAGGAGCGCATCGAGAGCGTGAACCTGCTGATACATCAGAGCCGTCTGGCGGCAATGGGGGAGA
TGATCGGCTACATAGCGCATCAGTGGCGTCAGCCTCTTAACATCATGGGCTTAATCGTTCAGGAAATGCCGATGGTGTAC
GGGGGTGGCGAATTCACGGAGGAATACCTGAAAGCGCAGATGGATAAGGCGAGGCAACTTGTTATGCACATGTCCGCAAC
TATCGACGATTTCAGGGATTTCTTCAAGCCGAGCAAGGAAAAAGAGGTCTTTAAGGCCGGGGAGATGATAAGGAAAACGC
TCGCCCTTATAGAAGGTGTCCTAAAAACCCAGCAGATCACGGTTCATGCAGACCAATCGGGGGATCCTGATATCTACGGC
TACATCGGAGAGTGCTGCCAAGTGCTGCTCAGCCTTCTGGTAAACTCGCGGGACGCCTTTCTGGCCCGCGAGGAGATAGC
GAACCGTGCCATCACGATCAGGACCTTTTCTGAGGACGGCAAGACGGTCCTCACTATCTCGGACAATGCGGGCGGAATAC
CCGAAGAGATTAGGGACAAGATCTTTGATGCCTATTTCACGACTAAACCCCCGGAGAAGGGGACCGGGCTCGGGCTGTCC
TTGGCGCGAAAGATAATCGAGAAACGGATGAACGGGAGGTTCACCTTTCGTGACATAGTTGACGGTGCAGAGTTCAGGAT
CGAGATTGACAGCAAACCCGCTGCCTGA

Upstream 100 bases:

>100_bases
CGCTTGCCTAAATTGCCTAAATATTCCTGGAGAGTTAGAATATCCCTTCTAGCAGCCATGCGTGATAGCGTGCAGCGGTG
AAGCCAAAAAGGTAGCTCTT

Downstream 100 bases:

>100_bases
AGGAGAAGACCAAGATGGATCTCACCTCAGGACAACATACGGATCCGGAAATACCCGGAAGGCGCGGGCGGACAGTGTAT
TCCTCCTTGCGGCAGTCATT

Product: PAS/PAC domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MEKLTITSRLLRMVTSALLVFLATVVGVKLLQPLGLRHPLLIYYPAVAVSAIYLGLEGGLTATLLSALSAAYFSIEPRSN
FAIRDFEEQLYVAVFVLEGCIISYAAYKMRRAETAEAQIKIAKERTLAAERLEESEIRLRLALEGAKMGMWTLDVTSNQC
ELSDQEYDLLGDTKGGGLEPVDAFFERVHPDDREEFKRLLSEVLDHGDNFSHEFRIVRPDGEIRWLAARARLSRSRKGKP
IRMHGVNYDVTSQRRFEEQLRHSENEARARANELAVLMDTVPAITFIAHDPECRDMSGSRKSREMLRVPEGKSVSASAPE
GEFANSFHGFKDGRELSPEELPVQMAARGQEVHDYELTLVFNDGTSCEVIGNAVPLFDGAGKARGAIGAFLDITEQKRIQ
RELEKVRDELDKRVKERTAELRQALETLQRETEERIESVNLLIHQSRLAAMGEMIGYIAHQWRQPLNIMGLIVQEMPMVY
GGGEFTEEYLKAQMDKARQLVMHMSATIDDFRDFFKPSKEKEVFKAGEMIRKTLALIEGVLKTQQITVHADQSGDPDIYG
YIGECCQVLLSLLVNSRDAFLAREEIANRAITIRTFSEDGKTVLTISDNAGGIPEEIRDKIFDAYFTTKPPEKGTGLGLS
LARKIIEKRMNGRFTFRDIVDGAEFRIEIDSKPAA

Sequences:

>Translated_675_residues
MEKLTITSRLLRMVTSALLVFLATVVGVKLLQPLGLRHPLLIYYPAVAVSAIYLGLEGGLTATLLSALSAAYFSIEPRSN
FAIRDFEEQLYVAVFVLEGCIISYAAYKMRRAETAEAQIKIAKERTLAAERLEESEIRLRLALEGAKMGMWTLDVTSNQC
ELSDQEYDLLGDTKGGGLEPVDAFFERVHPDDREEFKRLLSEVLDHGDNFSHEFRIVRPDGEIRWLAARARLSRSRKGKP
IRMHGVNYDVTSQRRFEEQLRHSENEARARANELAVLMDTVPAITFIAHDPECRDMSGSRKSREMLRVPEGKSVSASAPE
GEFANSFHGFKDGRELSPEELPVQMAARGQEVHDYELTLVFNDGTSCEVIGNAVPLFDGAGKARGAIGAFLDITEQKRIQ
RELEKVRDELDKRVKERTAELRQALETLQRETEERIESVNLLIHQSRLAAMGEMIGYIAHQWRQPLNIMGLIVQEMPMVY
GGGEFTEEYLKAQMDKARQLVMHMSATIDDFRDFFKPSKEKEVFKAGEMIRKTLALIEGVLKTQQITVHADQSGDPDIYG
YIGECCQVLLSLLVNSRDAFLAREEIANRAITIRTFSEDGKTVLTISDNAGGIPEEIRDKIFDAYFTTKPPEKGTGLGLS
LARKIIEKRMNGRFTFRDIVDGAEFRIEIDSKPAA
>Mature_675_residues
MEKLTITSRLLRMVTSALLVFLATVVGVKLLQPLGLRHPLLIYYPAVAVSAIYLGLEGGLTATLLSALSAAYFSIEPRSN
FAIRDFEEQLYVAVFVLEGCIISYAAYKMRRAETAEAQIKIAKERTLAAERLEESEIRLRLALEGAKMGMWTLDVTSNQC
ELSDQEYDLLGDTKGGGLEPVDAFFERVHPDDREEFKRLLSEVLDHGDNFSHEFRIVRPDGEIRWLAARARLSRSRKGKP
IRMHGVNYDVTSQRRFEEQLRHSENEARARANELAVLMDTVPAITFIAHDPECRDMSGSRKSREMLRVPEGKSVSASAPE
GEFANSFHGFKDGRELSPEELPVQMAARGQEVHDYELTLVFNDGTSCEVIGNAVPLFDGAGKARGAIGAFLDITEQKRIQ
RELEKVRDELDKRVKERTAELRQALETLQRETEERIESVNLLIHQSRLAAMGEMIGYIAHQWRQPLNIMGLIVQEMPMVY
GGGEFTEEYLKAQMDKARQLVMHMSATIDDFRDFFKPSKEKEVFKAGEMIRKTLALIEGVLKTQQITVHADQSGDPDIYG
YIGECCQVLLSLLVNSRDAFLAREEIANRAITIRTFSEDGKTVLTISDNAGGIPEEIRDKIFDAYFTTKPPEKGTGLGLS
LARKIIEKRMNGRFTFRDIVDGAEFRIEIDSKPAA

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76008; Mature: 76008

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLTITSRLLRMVTSALLVFLATVVGVKLLQPLGLRHPLLIYYPAVAVSAIYLGLEGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHCCCCCH
TATLLSALSAAYFSIEPRSNFAIRDFEEQLYVAVFVLEGCIISYAAYKMRRAETAEAQIK
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEE
IAKERTLAAERLEESEIRLRLALEGAKMGMWTLDVTSNQCELSDQEYDLLGDTKGGGLEP
HHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
VDAFFERVHPDDREEFKRLLSEVLDHGDNFSHEFRIVRPDGEIRWLAARARLSRSRKGKP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCCCC
IRMHGVNYDVTSQRRFEEQLRHSENEARARANELAVLMDTVPAITFIAHDPECRDMSGSR
EEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCH
KSREMLRVPEGKSVSASAPEGEFANSFHGFKDGRELSPEELPVQMAARGQEVHDYELTLV
HHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCHHHHHCCCEEEEEEEEEE
FNDGTSCEVIGNAVPLFDGAGKARGAIGAFLDITEQKRIQRELEKVRDELDKRVKERTAE
ECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQALETLQRETEERIESVNLLIHQSRLAAMGEMIGYIAHQWRQPLNIMGLIVQEMPMVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
GGGEFTEEYLKAQMDKARQLVMHMSATIDDFRDFFKPSKEKEVFKAGEMIRKTLALIEGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKTQQITVHADQSGDPDIYGYIGECCQVLLSLLVNSRDAFLAREEIANRAITIRTFSEDG
HHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCC
KTVLTISDNAGGIPEEIRDKIFDAYFTTKPPEKGTGLGLSLARKIIEKRMNGRFTFRDIV
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEHHHHC
DGAEFRIEIDSKPAA
CCCEEEEEECCCCCC
>Mature Secondary Structure
MEKLTITSRLLRMVTSALLVFLATVVGVKLLQPLGLRHPLLIYYPAVAVSAIYLGLEGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHHHCCCCCH
TATLLSALSAAYFSIEPRSNFAIRDFEEQLYVAVFVLEGCIISYAAYKMRRAETAEAQIK
HHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEE
IAKERTLAAERLEESEIRLRLALEGAKMGMWTLDVTSNQCELSDQEYDLLGDTKGGGLEP
HHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
VDAFFERVHPDDREEFKRLLSEVLDHGDNFSHEFRIVRPDGEIRWLAARARLSRSRKGKP
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEHHHHHHHHHHCCCCC
IRMHGVNYDVTSQRRFEEQLRHSENEARARANELAVLMDTVPAITFIAHDPECRDMSGSR
EEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCH
KSREMLRVPEGKSVSASAPEGEFANSFHGFKDGRELSPEELPVQMAARGQEVHDYELTLV
HHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHCCHHHHHCCCEEEEEEEEEE
FNDGTSCEVIGNAVPLFDGAGKARGAIGAFLDITEQKRIQRELEKVRDELDKRVKERTAE
ECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQALETLQRETEERIESVNLLIHQSRLAAMGEMIGYIAHQWRQPLNIMGLIVQEMPMVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEE
GGGEFTEEYLKAQMDKARQLVMHMSATIDDFRDFFKPSKEKEVFKAGEMIRKTLALIEGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKTQQITVHADQSGDPDIYGYIGECCQVLLSLLVNSRDAFLAREEIANRAITIRTFSEDG
HHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEEEECCCC
KTVLTISDNAGGIPEEIRDKIFDAYFTTKPPEKGTGLGLSLARKIIEKRMNGRFTFRDIV
CEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEHHHHC
DGAEFRIEIDSKPAA
CCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]