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The map label for this gene is hyfR [H]

Identifier: 197118899

GI number: 197118899

Start: 2923267

End: 2924796

Strand: Reverse

Name: hyfR [H]

Synonym: Gbem_2522

Alternate gene names: 197118899

Gene position: 2924796-2923267 (Counterclockwise)

Preceding gene: 197118907

Following gene: 197118897

Centisome position: 63.37

GC content: 56.21

Gene sequence:

>1530_bases
ATGGCTGTCGAAAAAGACGAATTCTTCAGAGAAGTGACGCTCAGGATCTGCAGCAGCCTGCAGATCAACATCGCCTTCGA
GAGGGCTTTCGAGTACTTGCGGGATCACTTCCCAATCGATCACATGTTCCTCGATATACCGGACAAGAGCCTGGGGGCCA
CCCGTCGTATCGCGTTCGCCTCGGTAGGCCACAGGCCGCCGGTGGAGATCGTCCCGCTTCCAGAAGGGGTCTGGGAGTGG
CTCGGCGGACTGCGCGCTCCCTTTATACTGAACGCTTCGGATGCGGACGAGAGGATCCGTACCTTCGCCCCCTTAATCAA
GCTTGAGGGGTGCTCGGACCTATCCATGCCTCTGCGTATCGAAGACGAACTTATCGGCTTCCTGATCCTGCGGGCAAAAG
GCGAAAAGAGGTACACATCAGACCACATGGCACTGCTTTCGGTGGTTGTCGAGCCCTTTGCCATGGCCCTTGCCAACGCA
TTAGCCCACGAAGCCGTGGTCAGATACCGGGACATACTCTTGGACGACAACCGCTTCCTGCGCAGCGAACTGGCGCCAAA
GTTGGGGGACGAGATCATCGGCGGGAACACGGGGCTGAGAAACGTCATGGAAATGGTCCAACAGGTGGCTCCGCTTTCCA
ATACGGTGCTCTTGATGGGAGAAACCGGGACCGGGAAAGAAGTGATCGCCAATGCCATCCACTTTGCTTCGCCGCGCAAG
GACGGCCCCTTCATCAAGGTGAACTGCGGCGCCATCCCAGAGGGCCTTATAGATAGCGAGCTTTTCGGCCACGAAAAGGG
GGCCTTTACCGGAGCAGTCACCGAAAAGCGTGGCCGCTTCGAGCGGGCAACTGGGGGGACCATCTTTCTCGATGAAATCG
GGGAGCTGCCGCCTCAGGCGCAGGTGAGGCTCTTGCGGGTGCTTCAGCAAAGGGAAATAGAACGGGTGGGAGGGGATAGG
CCTATCCCAGTGGATATCAGGGTGATAGCGGCGACCCACCGAAACCTCGAGAGCATGGTCACCGAAAACCGGTTCAGGGA
GGATCTGTGGTTCAGATTGAACGTGTTTCCGATAATCGTGCCGCCGCTAAGACAAAGAAGAGAAGATGTTCCTGCTCTCG
CCCGACATTTTGTGATGGTTAAATGCCGTGAGCTTGGCATAGCCATCCCACCCGCCATTGTGCCAGGTGCGTTGGAGAGA
CTGATGAACTACGGGTGGCCGGGGAACGTCAGGGAGCTGGAAAACTTGGTGGAGAGGGAGTTGATCCGGCATCGAGGAGG
GCAGTTAAGATTCGATTCTCTGCTGTCGGATGACAAGGGTGGCGAATTTAAACATGTAATTGAAGGTAAAGCTGCTGGCC
ATATAAGTCTTGATGAAGCCATGACCGCGCATATCAGCAACGTCCTAAAATTGACGAATGGGAAGATACATGGGCCGGGA
GGTGCGGCAGAACTACTCGGTATCAACCCAAATACCTTGAGAGGGCGGATGGGCAAACTCGGTTTGAAGTCTGGCAGGAG
GACTGGATAG

Upstream 100 bases:

>100_bases
AATCCGGTAATTTGCTGAATATGACAATTTCAAAACACGTCACTGACAGCATAGCGTTACAGTGGATGAGCAATTCTGTT
TAGCGGAGCGTTTAATTATT

Downstream 100 bases:

>100_bases
CTTTTGGCTGCTAGAAGTTGATGCTGGTTTTTTGGGGGGCGGGGGCGGCAAGGAGAGGGGGGGGCGACGACCGGTGGAGA
AGACGTCTTCTGGGCGCCGC

Product: GAF sensor sigma-54-dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MAVEKDEFFREVTLRICSSLQINIAFERAFEYLRDHFPIDHMFLDIPDKSLGATRRIAFASVGHRPPVEIVPLPEGVWEW
LGGLRAPFILNASDADERIRTFAPLIKLEGCSDLSMPLRIEDELIGFLILRAKGEKRYTSDHMALLSVVVEPFAMALANA
LAHEAVVRYRDILLDDNRFLRSELAPKLGDEIIGGNTGLRNVMEMVQQVAPLSNTVLLMGETGTGKEVIANAIHFASPRK
DGPFIKVNCGAIPEGLIDSELFGHEKGAFTGAVTEKRGRFERATGGTIFLDEIGELPPQAQVRLLRVLQQREIERVGGDR
PIPVDIRVIAATHRNLESMVTENRFREDLWFRLNVFPIIVPPLRQRREDVPALARHFVMVKCRELGIAIPPAIVPGALER
LMNYGWPGNVRELENLVERELIRHRGGQLRFDSLLSDDKGGEFKHVIEGKAAGHISLDEAMTAHISNVLKLTNGKIHGPG
GAAELLGINPNTLRGRMGKLGLKSGRRTG

Sequences:

>Translated_509_residues
MAVEKDEFFREVTLRICSSLQINIAFERAFEYLRDHFPIDHMFLDIPDKSLGATRRIAFASVGHRPPVEIVPLPEGVWEW
LGGLRAPFILNASDADERIRTFAPLIKLEGCSDLSMPLRIEDELIGFLILRAKGEKRYTSDHMALLSVVVEPFAMALANA
LAHEAVVRYRDILLDDNRFLRSELAPKLGDEIIGGNTGLRNVMEMVQQVAPLSNTVLLMGETGTGKEVIANAIHFASPRK
DGPFIKVNCGAIPEGLIDSELFGHEKGAFTGAVTEKRGRFERATGGTIFLDEIGELPPQAQVRLLRVLQQREIERVGGDR
PIPVDIRVIAATHRNLESMVTENRFREDLWFRLNVFPIIVPPLRQRREDVPALARHFVMVKCRELGIAIPPAIVPGALER
LMNYGWPGNVRELENLVERELIRHRGGQLRFDSLLSDDKGGEFKHVIEGKAAGHISLDEAMTAHISNVLKLTNGKIHGPG
GAAELLGINPNTLRGRMGKLGLKSGRRTG
>Mature_508_residues
AVEKDEFFREVTLRICSSLQINIAFERAFEYLRDHFPIDHMFLDIPDKSLGATRRIAFASVGHRPPVEIVPLPEGVWEWL
GGLRAPFILNASDADERIRTFAPLIKLEGCSDLSMPLRIEDELIGFLILRAKGEKRYTSDHMALLSVVVEPFAMALANAL
AHEAVVRYRDILLDDNRFLRSELAPKLGDEIIGGNTGLRNVMEMVQQVAPLSNTVLLMGETGTGKEVIANAIHFASPRKD
GPFIKVNCGAIPEGLIDSELFGHEKGAFTGAVTEKRGRFERATGGTIFLDEIGELPPQAQVRLLRVLQQREIERVGGDRP
IPVDIRVIAATHRNLESMVTENRFREDLWFRLNVFPIIVPPLRQRREDVPALARHFVMVKCRELGIAIPPAIVPGALERL
MNYGWPGNVRELENLVERELIRHRGGQLRFDSLLSDDKGGEFKHVIEGKAAGHISLDEAMTAHISNVLKLTNGKIHGPGG
AAELLGINPNTLRGRMGKLGLKSGRRTG

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=412, Percent_Identity=44.9029126213592, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1789087, Length=392, Percent_Identity=43.6224489795918, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790299, Length=377, Percent_Identity=41.9098143236074, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788550, Length=235, Percent_Identity=52.7659574468085, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1790437, Length=231, Percent_Identity=51.0822510822511, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=42.2360248447205, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789233, Length=275, Percent_Identity=43.6363636363636, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1788905, Length=218, Percent_Identity=49.0825688073394, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=49.1525423728814, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=48.7704918032787, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=316, Percent_Identity=37.3417721518987, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87081858, Length=310, Percent_Identity=33.2258064516129, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1789828, Length=294, Percent_Identity=34.6938775510204, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 56633; Mature: 56502

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEKDEFFREVTLRICSSLQINIAFERAFEYLRDHFPIDHMFLDIPDKSLGATRRIAFA
CCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
SVGHRPPVEIVPLPEGVWEWLGGLRAPFILNASDADERIRTFAPLIKLEGCSDLSMPLRI
HCCCCCCEEEEECCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCCCCEEE
EDELIGFLILRAKGEKRYTSDHMALLSVVVEPFAMALANALAHEAVVRYRDILLDDNRFL
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RSELAPKLGDEIIGGNTGLRNVMEMVQQVAPLSNTVLLMGETGTGKEVIANAIHFASPRK
HHHHCHHHCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCC
DGPFIKVNCGAIPEGLIDSELFGHEKGAFTGAVTEKRGRFERATGGTIFLDEIGELPPQA
CCCEEEEECCCCCCCHHHHHHHCCCCCCEECCHHHHCCCCCCCCCCEEEHHHHCCCCCHH
QVRLLRVLQQREIERVGGDRPIPVDIRVIAATHRNLESMVTENRFREDLWFRLNVFPIIV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHEEEEEEEEEEC
PPLRQRREDVPALARHFVMVKCRELGIAIPPAIVPGALERLMNYGWPGNVRELENLVERE
CCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LIRHRGGQLRFDSLLSDDKGGEFKHVIEGKAAGHISLDEAMTAHISNVLKLTNGKIHGPG
HHHHCCCCEEEHHHHCCCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCC
GAAELLGINPNTLRGRMGKLGLKSGRRTG
CCEEEEECCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AVEKDEFFREVTLRICSSLQINIAFERAFEYLRDHFPIDHMFLDIPDKSLGATRRIAFA
CCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
SVGHRPPVEIVPLPEGVWEWLGGLRAPFILNASDADERIRTFAPLIKLEGCSDLSMPLRI
HCCCCCCEEEEECCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHEECCCCCCCCCEEE
EDELIGFLILRAKGEKRYTSDHMALLSVVVEPFAMALANALAHEAVVRYRDILLDDNRFL
HHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RSELAPKLGDEIIGGNTGLRNVMEMVQQVAPLSNTVLLMGETGTGKEVIANAIHFASPRK
HHHHCHHHCCHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCC
DGPFIKVNCGAIPEGLIDSELFGHEKGAFTGAVTEKRGRFERATGGTIFLDEIGELPPQA
CCCEEEEECCCCCCCHHHHHHHCCCCCCEECCHHHHCCCCCCCCCCEEEHHHHCCCCCHH
QVRLLRVLQQREIERVGGDRPIPVDIRVIAATHRNLESMVTENRFREDLWFRLNVFPIIV
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHEEEEEEEEEEC
PPLRQRREDVPALARHFVMVKCRELGIAIPPAIVPGALERLMNYGWPGNVRELENLVERE
CCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LIRHRGGQLRFDSLLSDDKGGEFKHVIEGKAAGHISLDEAMTAHISNVLKLTNGKIHGPG
HHHHCCCCEEEHHHHCCCCCCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCEEECCC
GAAELLGINPNTLRGRMGKLGLKSGRRTG
CCEEEEECCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]