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The map label for this gene is rfbA [H]

Identifier: 197118841

GI number: 197118841

Start: 2853061

End: 2853966

Strand: Reverse

Name: rfbA [H]

Synonym: Gbem_2464

Alternate gene names: 197118841

Gene position: 2853966-2853061 (Counterclockwise)

Preceding gene: 197118842

Following gene: 197118840

Centisome position: 61.84

GC content: 61.37

Gene sequence:

>906_bases
ATGCCACAAGGTATCAAGAAGGGGATTCTCCTGGCCGGAGGCGCGGGAAGCAGGCTTTACCCTCTGACCATGGTAGCGAG
CAAGCAGCTGCAGCCGGTCTACGACAAGCCGATGATCTATTACCCGCTGGCGACGCTGATGATGGCGGGAATCGCCGACG
TGCTGATCATTTCCACTCCGCAGGACACCCCCCGCTTCCAGGCGCTATTGGGCGACGGCTCCCGCTGGGGCATCCGTCTT
TCCTACGCGGTGCAGCAAGAACCCAAGGGGATCGCGCAGGCGTTTCTCGTCGGGGAGGAGTTCATCGCCGGCGATCCGGT
CTGCCTGATTCTGGGCGACAACATCTTCTACGGCAAAATGGAATTGGATCGTCTCATCACCGGTTTTGACGGCGGCGCCA
GGATTTTCGGCTACTACGTCCAGGATCCGGAGCGTTACGGCGTGGTCGAGTTCGACCGAGAAGACCGGGCGCTCAGCATC
GAAGAGAAGCCGGCCCATCCCAAGTCGAACTACGCGGTCCCAGGGCTGTACCTCTACGACGGCAAGGTTGTGCAGATCGC
CAAGGAGTTGGAGCCCTCGCCGCGGGGCGAGCTGGAAATCACGGATGTGAACCTGGAGTACTTAAGGCGCGGCGAACTTT
CGGTGCAAAGGCTCGGGCGCGGCATCGCCTGGCTCGACACCGGAACCCACCAAAGCCTTCTCGAGGCGTCCCACTTCATA
GGAACCCTGGAGGCGCGGCAGGGACTGAAGATCGCTTGCCTGGAGGAGATAGCGCTCCGGATGGGGTACCTTGACTGCGC
GGCGATGGCCAAGGTCATCGATGCCACCCCCAACTCCTCGTATCGGGACTATCTGGTGCGGGTTTACAACGAGACTACGC
TTTGCGGGGGAGGGATGGGAGCATGA

Upstream 100 bases:

>100_bases
GCAACCTGTGATTGCACCCTTTATTGGTTTGACGCAGGACGCGCCTGTGTGATAGAAAAAGTCTTTTGTTAATGTGATCG
GAGATTTGAAAGGTTCATTT

Downstream 100 bases:

>100_bases
GCGGGATAACCAGTTTCACCAAGGGGCGTATCCACGACGTGGTGCTTAAGCCGCTGGGGAAGTTCCTGGACGAGCGGGGG
TGGCTTTGCGAGCTTTTCCG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MPQGIKKGILLAGGAGSRLYPLTMVASKQLQPVYDKPMIYYPLATLMMAGIADVLIISTPQDTPRFQALLGDGSRWGIRL
SYAVQQEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMELDRLITGFDGGARIFGYYVQDPERYGVVEFDREDRALSI
EEKPAHPKSNYAVPGLYLYDGKVVQIAKELEPSPRGELEITDVNLEYLRRGELSVQRLGRGIAWLDTGTHQSLLEASHFI
GTLEARQGLKIACLEEIALRMGYLDCAAMAKVIDATPNSSYRDYLVRVYNETTLCGGGMGA

Sequences:

>Translated_301_residues
MPQGIKKGILLAGGAGSRLYPLTMVASKQLQPVYDKPMIYYPLATLMMAGIADVLIISTPQDTPRFQALLGDGSRWGIRL
SYAVQQEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMELDRLITGFDGGARIFGYYVQDPERYGVVEFDREDRALSI
EEKPAHPKSNYAVPGLYLYDGKVVQIAKELEPSPRGELEITDVNLEYLRRGELSVQRLGRGIAWLDTGTHQSLLEASHFI
GTLEARQGLKIACLEEIALRMGYLDCAAMAKVIDATPNSSYRDYLVRVYNETTLCGGGMGA
>Mature_300_residues
PQGIKKGILLAGGAGSRLYPLTMVASKQLQPVYDKPMIYYPLATLMMAGIADVLIISTPQDTPRFQALLGDGSRWGIRLS
YAVQQEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKMELDRLITGFDGGARIFGYYVQDPERYGVVEFDREDRALSIE
EKPAHPKSNYAVPGLYLYDGKVVQIAKELEPSPRGELEITDVNLEYLRRGELSVQRLGRGIAWLDTGTHQSLLEASHFIG
TLEARQGLKIACLEEIALRMGYLDCAAMAKVIDATPNSSYRDYLVRVYNETTLCGGGMGA

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=253, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI11761619, Length=253, Percent_Identity=26.0869565217391, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1788351, Length=292, Percent_Identity=61.6438356164384, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1790224, Length=284, Percent_Identity=58.4507042253521, Blast_Score=342, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI133931050, Length=199, Percent_Identity=26.1306532663317, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=305, Percent_Identity=25.5737704918033, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355443, Length=253, Percent_Identity=22.9249011857708, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644084, Length=253, Percent_Identity=22.9249011857708, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 33124; Mature: 32992

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQGIKKGILLAGGAGSRLYPLTMVASKQLQPVYDKPMIYYPLATLMMAGIADVLIISTP
CCCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECC
QDTPRFQALLGDGSRWGIRLSYAVQQEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKM
CCCCHHHHEECCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCEEEEEECCEEECCE
ELDRLITGFDGGARIFGYYVQDPERYGVVEFDREDRALSIEEKPAHPKSNYAVPGLYLYD
EHHHHHHCCCCCCEEEEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCEEEEEC
GKVVQIAKELEPSPRGELEITDVNLEYLRRGELSVQRLGRGIAWLDTGTHQSLLEASHFI
CCCEEEHHHCCCCCCCCEEEEECCHHHHHCCCCCHHHHCCCEEEEECCCHHHHHHHHHHH
GTLEARQGLKIACLEEIALRMGYLDCAAMAKVIDATPNSSYRDYLVRVYNETTLCGGGMG
EEHHHCCCCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHEEECCCEECCCCCC
A
C
>Mature Secondary Structure 
PQGIKKGILLAGGAGSRLYPLTMVASKQLQPVYDKPMIYYPLATLMMAGIADVLIISTP
CCCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECC
QDTPRFQALLGDGSRWGIRLSYAVQQEPKGIAQAFLVGEEFIAGDPVCLILGDNIFYGKM
CCCCHHHHEECCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCEEEEEECCEEECCE
ELDRLITGFDGGARIFGYYVQDPERYGVVEFDREDRALSIEEKPAHPKSNYAVPGLYLYD
EHHHHHHCCCCCCEEEEEEEECCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCEEEEEC
GKVVQIAKELEPSPRGELEITDVNLEYLRRGELSVQRLGRGIAWLDTGTHQSLLEASHFI
CCCEEEHHHCCCCCCCCEEEEECCHHHHHCCCCCHHHHCCCEEEEECCCHHHHHHHHHHH
GTLEARQGLKIACLEEIALRMGYLDCAAMAKVIDATPNSSYRDYLVRVYNETTLCGGGMG
EEHHHCCCCEEEHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHHEEECCCEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]