Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 197118786

GI number: 197118786

Start: 2786884

End: 2787927

Strand: Direct

Name: rpfG [H]

Synonym: Gbem_2407

Alternate gene names: 197118786

Gene position: 2786884-2787927 (Clockwise)

Preceding gene: 197118785

Following gene: 197118793

Centisome position: 60.39

GC content: 58.91

Gene sequence:

>1044_bases
TTGAAAGGAAACATCTTAATAGCCGATGACGAGGACATGATCAGGGAACTGATCAACATCACCCTGTCAAAGGAAGGCTT
CACCTGCTTCCAGGCCGCAAGCGCCGAGGAGGGGTTGGAGATAATCAACAAGCACAAGCTGGACCTGGCGCTTTTAGACA
TCATGATGCCAGGCCGCTCGGGAATCGACCTCTTGAAGGACATCAAGGAGGCCACCCCCGACACGACGGTGCTGATGATA
ACGGCGATGAACGACATGGACACCGCCCTTTCCTGCATCCATTACGGCGCCGAGGACTACATCACCAAGCCGTTCAACCT
GGACCGGGTGCTCCTCACCGTGAAGAACACCCTGGAAAAACGCAGGCTGGTGCTGGAGAACAAGGAATACCAGGCGAACC
TGGAACAGAAGGTGCGCGAGCAGACCGAGGTGATCCGCACCGTGATGGGGGAGATCAACCTCGCCTACGAACACACGCTG
GTGGCGCTGATCAGGGCGCTGGACGCGAGGGAAAAGGAAGTGGGATCCCACTCCGAGCGGGTCATGTCCTACGCCAGGCT
TTTGGCGAAAGCCGCGGGGATCAGCGAGGACGAGCTGATCATCATAGGCAAAGGCGCGCTTTTGCACGACATCGGAAAGA
TCGGCGTCTCCGACAACATCCTGTTGAAGCCCGCGAAGCTAGACGACTCGGAGTGGGAGATCATGCGCCAGCACCCCAGC
ATCGGCTTCGACATACTGTCCGGCATCCGGTACTTCGCCGGGGCGGCGGAACTGGTGCTGCATCATCACGAGCGCTGGGA
CGGAAACGGCTATCCGGGCAACCTGGAAGGGGAAAACATCCCGATCAGCGCGCGCATCTTCGCCCTGGTGGACACGCTCG
ATGCCATGACTTCCGACCGGCCCTACCGCAAGGCATTGACCTTCGAGGCGGTTCTGGACGAAGTAACGCGTTGCCGCAGC
AAGCAGTTCGATCCCGAGCTGGTCGATCTGTTCCTCAGCATTCCGAAGGACGAGTGGGAAAGCGCGGCGGGGAAGAAACT
GTAA

Upstream 100 bases:

>100_bases
TGCCCAAACCGTTTGCCAGCGACGCCCTGATCCGAAAGGTGAGGGACACCCTGAACGGAGGCACTGCAAAGACGGCAGGA
GCGATTTCAGGAGGTAGCGA

Downstream 100 bases:

>100_bases
CGACTCTCCCTTCTTACCGAGCGCAGTACGAAAAAAGCCGGAACGAACCCAGTGTGGGGTTCATTCCGGCTTTTCTTTAT
CAGGTACCCGCCTTGCGGCA

Product: response receiver-modulated cyclic diguanylate phosphodiesterase

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKGNILIADDEDMIRELINITLSKEGFTCFQAASAEEGLEIINKHKLDLALLDIMMPGRSGIDLLKDIKEATPDTTVLMI
TAMNDMDTALSCIHYGAEDYITKPFNLDRVLLTVKNTLEKRRLVLENKEYQANLEQKVREQTEVIRTVMGEINLAYEHTL
VALIRALDAREKEVGSHSERVMSYARLLAKAAGISEDELIIIGKGALLHDIGKIGVSDNILLKPAKLDDSEWEIMRQHPS
IGFDILSGIRYFAGAAELVLHHHERWDGNGYPGNLEGENIPISARIFALVDTLDAMTSDRPYRKALTFEAVLDEVTRCRS
KQFDPELVDLFLSIPKDEWESAAGKKL

Sequences:

>Translated_347_residues
MKGNILIADDEDMIRELINITLSKEGFTCFQAASAEEGLEIINKHKLDLALLDIMMPGRSGIDLLKDIKEATPDTTVLMI
TAMNDMDTALSCIHYGAEDYITKPFNLDRVLLTVKNTLEKRRLVLENKEYQANLEQKVREQTEVIRTVMGEINLAYEHTL
VALIRALDAREKEVGSHSERVMSYARLLAKAAGISEDELIIIGKGALLHDIGKIGVSDNILLKPAKLDDSEWEIMRQHPS
IGFDILSGIRYFAGAAELVLHHHERWDGNGYPGNLEGENIPISARIFALVDTLDAMTSDRPYRKALTFEAVLDEVTRCRS
KQFDPELVDLFLSIPKDEWESAAGKKL
>Mature_347_residues
MKGNILIADDEDMIRELINITLSKEGFTCFQAASAEEGLEIINKHKLDLALLDIMMPGRSGIDLLKDIKEATPDTTVLMI
TAMNDMDTALSCIHYGAEDYITKPFNLDRVLLTVKNTLEKRRLVLENKEYQANLEQKVREQTEVIRTVMGEINLAYEHTL
VALIRALDAREKEVGSHSERVMSYARLLAKAAGISEDELIIIGKGALLHDIGKIGVSDNILLKPAKLDDSEWEIMRQHPS
IGFDILSGIRYFAGAAELVLHHHERWDGNGYPGNLEGENIPISARIFALVDTLDAMTSDRPYRKALTFEAVLDEVTRCRS
KQFDPELVDLFLSIPKDEWESAAGKKL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=34.7826086956522, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=34.4262295081967, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=27.9661016949153, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790566, Length=139, Percent_Identity=35.9712230215827, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790863, Length=117, Percent_Identity=29.9145299145299, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786911, Length=104, Percent_Identity=29.8076923076923, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=32.1739130434783, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788905, Length=118, Percent_Identity=27.9661016949153, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787229, Length=118, Percent_Identity=30.5084745762712, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786784, Length=128, Percent_Identity=27.34375, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=30.1724137931034, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789402, Length=104, Percent_Identity=31.7307692307692, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38933; Mature: 38933

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGNILIADDEDMIRELINITLSKEGFTCFQAASAEEGLEIINKHKLDLALLDIMMPGRS
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIDLLKDIKEATPDTTVLMITAMNDMDTALSCIHYGAEDYITKPFNLDRVLLTVKNTLEK
CHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHH
RRLVLENKEYQANLEQKVREQTEVIRTVMGEINLAYEHTLVALIRALDAREKEVGSHSER
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VMSYARLLAKAAGISEDELIIIGKGALLHDIGKIGVSDNILLKPAKLDDSEWEIMRQHPS
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCC
IGFDILSGIRYFAGAAELVLHHHERWDGNGYPGNLEGENIPISARIFALVDTLDAMTSDR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRKALTFEAVLDEVTRCRSKQFDPELVDLFLSIPKDEWESAAGKKL
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MKGNILIADDEDMIRELINITLSKEGFTCFQAASAEEGLEIINKHKLDLALLDIMMPGRS
CCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIDLLKDIKEATPDTTVLMITAMNDMDTALSCIHYGAEDYITKPFNLDRVLLTVKNTLEK
CHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHH
RRLVLENKEYQANLEQKVREQTEVIRTVMGEINLAYEHTLVALIRALDAREKEVGSHSER
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
VMSYARLLAKAAGISEDELIIIGKGALLHDIGKIGVSDNILLKPAKLDDSEWEIMRQHPS
HHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHCCC
IGFDILSGIRYFAGAAELVLHHHERWDGNGYPGNLEGENIPISARIFALVDTLDAMTSDR
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRKALTFEAVLDEVTRCRSKQFDPELVDLFLSIPKDEWESAAGKKL
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]