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The map label for this gene is btuB [H]

Identifier: 197118768

GI number: 197118768

Start: 2767906

End: 2769753

Strand: Reverse

Name: btuB [H]

Synonym: Gbem_2389

Alternate gene names: 197118768

Gene position: 2769753-2767906 (Counterclockwise)

Preceding gene: 308535302

Following gene: 197118767

Centisome position: 60.01

GC content: 60.01

Gene sequence:

>1848_bases
TTGCAAAAAGCCATTGCCATTGTTCAGTCCGCTTTTGCCAGAGCTAAGGTCCCTCTGATAGTGCAGATGCTGTTTTTGAT
CATTGCCGTCTCTCAGGCGAGGGGGGACGAAGAGCTTCGTTCTTTGGAACTCTACAATGGCGACGAGGCCGAGATAGTGT
CGGGGAGCAGGTCCCCAAGGCCGGCCTCCCAGACCGCCGAGAACATAACCGTGGTGACTTCCGAGGACATATTGGCGCTT
AACGCCCACACCCTGGCGGACATACTCTATGCCGTAGCCGGGGTACAGGTGGAGATGATCCGCACGCCGGGGGCCTCCAC
CAACTTCAAGCTGCAGGGGGCCAACTTCAACCACGTGCTGGTATTGATAGACAACGTCCCCCTCAATACGCTCTCGGAGA
ATTACCCAGACATCGCCGCAGTCCCGGTGCAGATGATCGAGAGGGTGGAAATAGTGAAGGGAGCCGCGTCGTCTGCATGG
GGCAACGCCTTGGGCGGGGTGATCAACGTCATGACCAAGTCCCCGGATCAAGAGCGTGCCATCGGCGGCATGGCGTCCGG
GTCGTACGGCAAACGGGACACCGCGGACCTGCGGGGGGAGTTGAGCGGCACGGTGGACAGTTTCGGCTACTACCTGTCCG
GCGGGAAGCTGCGGTCGGACGGCCTTCTTGCCAACAACGGGGTCGACAAGGACAACTTCTACGGCAAGTTGCTGTACGAC
CTCCCAAACGGCGGCTCTCTCGCCTTGACCACCTGGCTTAGCCAGGGCTTCAACGGCATGTACCAGGACGGTCCCGTCTC
CACCAGCCAGAACACGCGGTATCTGATCAGCACCTTGGCCGCCAGCTATCAGCTGACCGACCACCTGCGTCTCGAAGCTG
CGGCGGTAGCTTCGGAAACTGAGGGAACCCTTTTTCGCCGGACGGACCTGCAGGGCGTTTCCAAGACATTAACCGTTGCC
CTGGACGAGTCCAGGGTAGGAGGGGGCTTCAAGCTCTCGTGGCTGGACGAGTTGCAGAGGGTGGTGGCCGGGGTCGATTA
CGAGCACGTGGCGGCGCAGGTGAGCAGCTCACAGATCAGGGCCGACATCCTGAACCGAGACGCCGATCGGTTCGGCGTCT
ACCTGAGCGACACGCTGACGCTCGGCCGCTTTGCCGTCACCCCCAGTGCGCGCTTTGACAGGACGGGCTCAGGAGGCGAC
CACTTCAGCCCAAGCTTCGGCGTTACCTACGCGCTCACCGACAACAGCATTCTTCGCGGCTACACCGCCACGGGATACAG
CCTCACCTCGCTTAACCGCTCGGACTCCACGGAAAAGGTCTGGACCTCGCAGTTCGGCTTCGAGTCCGGCGACCTTCCCT
ACCTCTGGGTCAAGGGAACCCTGTTCAGAAACGACACCTGGGATGTCATCGCGCCGACTGCGACCGGCATTACTAAGGAG
CGGCAGCTGAAGCAGGGTGTAGAACTTGAGGCGAAGACCCTTCCTCTGTACAACACCTCCCTTTCGGTTGGGTACACCTT
CATCGGCGCCACCCTGGGCGTCAATGGCCCGGTCATCAAGGGGGTGCCCAAGCACACCGTCAACATCGGGCTCAGATACC
AGGACCCCAGCCACTTTCGCGGAGAACTCACCGGGCACTACGTGGACTGGAACGAGGCTAGGCAAGGAGGTAGATACAGC
GACGTCATATGGGACCTGCACCTAAGAAAGGAAATGCATTACGGGGAGAAGGTTTCCCTTGAGCCGTTTGTTTCGGTTCG
CAACCTGTTGAACGGACACCAGTATGTGTACCGCGTATACCAGAATCCCGGGCGCTGGATAGAGCTGGGGGTAAGATGCA
ATTTCTGA

Upstream 100 bases:

>100_bases
ATGTCAAAAATCTTGTCCTTGCTCGATAGGTTATGGTAAGTTAACACGCCTTTAATCAGTTTGCAATAATATATTTTATT
TTTTTAAGGTTGGAGGGGAA

Downstream 100 bases:

>100_bases
GATTCCTCTTGCTCGCCTTGCTGTTGTTGATCCCGCTTCCCGCATTTGGCTACGACGTACTGGTGTTGCAGTCCCTGCAC
GAGAAGGGGTACGAAGAGGC

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MQKAIAIVQSAFARAKVPLIVQMLFLIIAVSQARGDEELRSLELYNGDEAEIVSGSRSPRPASQTAENITVVTSEDILAL
NAHTLADILYAVAGVQVEMIRTPGASTNFKLQGANFNHVLVLIDNVPLNTLSENYPDIAAVPVQMIERVEIVKGAASSAW
GNALGGVINVMTKSPDQERAIGGMASGSYGKRDTADLRGELSGTVDSFGYYLSGGKLRSDGLLANNGVDKDNFYGKLLYD
LPNGGSLALTTWLSQGFNGMYQDGPVSTSQNTRYLISTLAASYQLTDHLRLEAAAVASETEGTLFRRTDLQGVSKTLTVA
LDESRVGGGFKLSWLDELQRVVAGVDYEHVAAQVSSSQIRADILNRDADRFGVYLSDTLTLGRFAVTPSARFDRTGSGGD
HFSPSFGVTYALTDNSILRGYTATGYSLTSLNRSDSTEKVWTSQFGFESGDLPYLWVKGTLFRNDTWDVIAPTATGITKE
RQLKQGVELEAKTLPLYNTSLSVGYTFIGATLGVNGPVIKGVPKHTVNIGLRYQDPSHFRGELTGHYVDWNEARQGGRYS
DVIWDLHLRKEMHYGEKVSLEPFVSVRNLLNGHQYVYRVYQNPGRWIELGVRCNF

Sequences:

>Translated_615_residues
MQKAIAIVQSAFARAKVPLIVQMLFLIIAVSQARGDEELRSLELYNGDEAEIVSGSRSPRPASQTAENITVVTSEDILAL
NAHTLADILYAVAGVQVEMIRTPGASTNFKLQGANFNHVLVLIDNVPLNTLSENYPDIAAVPVQMIERVEIVKGAASSAW
GNALGGVINVMTKSPDQERAIGGMASGSYGKRDTADLRGELSGTVDSFGYYLSGGKLRSDGLLANNGVDKDNFYGKLLYD
LPNGGSLALTTWLSQGFNGMYQDGPVSTSQNTRYLISTLAASYQLTDHLRLEAAAVASETEGTLFRRTDLQGVSKTLTVA
LDESRVGGGFKLSWLDELQRVVAGVDYEHVAAQVSSSQIRADILNRDADRFGVYLSDTLTLGRFAVTPSARFDRTGSGGD
HFSPSFGVTYALTDNSILRGYTATGYSLTSLNRSDSTEKVWTSQFGFESGDLPYLWVKGTLFRNDTWDVIAPTATGITKE
RQLKQGVELEAKTLPLYNTSLSVGYTFIGATLGVNGPVIKGVPKHTVNIGLRYQDPSHFRGELTGHYVDWNEARQGGRYS
DVIWDLHLRKEMHYGEKVSLEPFVSVRNLLNGHQYVYRVYQNPGRWIELGVRCNF
>Mature_615_residues
MQKAIAIVQSAFARAKVPLIVQMLFLIIAVSQARGDEELRSLELYNGDEAEIVSGSRSPRPASQTAENITVVTSEDILAL
NAHTLADILYAVAGVQVEMIRTPGASTNFKLQGANFNHVLVLIDNVPLNTLSENYPDIAAVPVQMIERVEIVKGAASSAW
GNALGGVINVMTKSPDQERAIGGMASGSYGKRDTADLRGELSGTVDSFGYYLSGGKLRSDGLLANNGVDKDNFYGKLLYD
LPNGGSLALTTWLSQGFNGMYQDGPVSTSQNTRYLISTLAASYQLTDHLRLEAAAVASETEGTLFRRTDLQGVSKTLTVA
LDESRVGGGFKLSWLDELQRVVAGVDYEHVAAQVSSSQIRADILNRDADRFGVYLSDTLTLGRFAVTPSARFDRTGSGGD
HFSPSFGVTYALTDNSILRGYTATGYSLTSLNRSDSTEKVWTSQFGFESGDLPYLWVKGTLFRNDTWDVIAPTATGITKE
RQLKQGVELEAKTLPLYNTSLSVGYTFIGATLGVNGPVIKGVPKHTVNIGLRYQDPSHFRGELTGHYVDWNEARQGGRYS
DVIWDLHLRKEMHYGEKVSLEPFVSVRNLLNGHQYVYRVYQNPGRWIELGVRCNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=147, Percent_Identity=31.9727891156463, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788478, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67177; Mature: 67177

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKAIAIVQSAFARAKVPLIVQMLFLIIAVSQARGDEELRSLELYNGDEAEIVSGSRSPR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCEEECCCCCCC
PASQTAENITVVTSEDILALNAHTLADILYAVAGVQVEMIRTPGASTNFKLQGANFNHVL
CCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCEEE
VLIDNVPLNTLSENYPDIAAVPVQMIERVEIVKGAASSAWGNALGGVINVMTKSPDQERA
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
IGGMASGSYGKRDTADLRGELSGTVDSFGYYLSGGKLRSDGLLANNGVDKDNFYGKLLYD
HCCCCCCCCCCCCCHHHHHHCCCCHHHCCEEEECCEECCCCEEECCCCCCCCCCEEEEEE
LPNGGSLALTTWLSQGFNGMYQDGPVSTSQNTRYLISTLAASYQLTDHLRLEAAAVASET
CCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHCCCC
EGTLFRRTDLQGVSKTLTVALDESRVGGGFKLSWLDELQRVVAGVDYEHVAAQVSSSQIR
CCCEEEECCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ADILNRDADRFGVYLSDTLTLGRFAVTPSARFDRTGSGGDHFSPSFGVTYALTDNSILRG
HHHHCCCHHHHCEEEECCEEECEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEC
YTATGYSLTSLNRSDSTEKVWTSQFGFESGDLPYLWVKGTLFRNDTWDVIAPTATGITKE
EEECCEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCHH
RQLKQGVELEAKTLPLYNTSLSVGYTFIGATLGVNGPVIKGVPKHTVNIGLRYQDPSHFR
HHHHCCCCEEEEEEEEEECCCCCCEEEEEHEECCCCCEECCCCCCEEEEEEEECCCCCCC
GELTGHYVDWNEARQGGRYSDVIWDLHLRKEMHYGEKVSLEPFVSVRNLLNGHQYVYRVY
CCEEEEEECHHHHHCCCCCHHEEEEEEHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEE
QNPGRWIELGVRCNF
CCCCCEEEEEEEECC
>Mature Secondary Structure
MQKAIAIVQSAFARAKVPLIVQMLFLIIAVSQARGDEELRSLELYNGDEAEIVSGSRSPR
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCCCEEECCCCCCC
PASQTAENITVVTSEDILALNAHTLADILYAVAGVQVEMIRTPGASTNFKLQGANFNHVL
CCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCEEE
VLIDNVPLNTLSENYPDIAAVPVQMIERVEIVKGAASSAWGNALGGVINVMTKSPDQERA
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHH
IGGMASGSYGKRDTADLRGELSGTVDSFGYYLSGGKLRSDGLLANNGVDKDNFYGKLLYD
HCCCCCCCCCCCCCHHHHHHCCCCHHHCCEEEECCEECCCCEEECCCCCCCCCCEEEEEE
LPNGGSLALTTWLSQGFNGMYQDGPVSTSQNTRYLISTLAASYQLTDHLRLEAAAVASET
CCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEHHHHCCCC
EGTLFRRTDLQGVSKTLTVALDESRVGGGFKLSWLDELQRVVAGVDYEHVAAQVSSSQIR
CCCEEEECCCCCCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ADILNRDADRFGVYLSDTLTLGRFAVTPSARFDRTGSGGDHFSPSFGVTYALTDNSILRG
HHHHCCCHHHHCEEEECCEEECEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEC
YTATGYSLTSLNRSDSTEKVWTSQFGFESGDLPYLWVKGTLFRNDTWDVIAPTATGITKE
EEECCEEEEECCCCCCCHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCEECCCCCCCCHH
RQLKQGVELEAKTLPLYNTSLSVGYTFIGATLGVNGPVIKGVPKHTVNIGLRYQDPSHFR
HHHHCCCCEEEEEEEEEECCCCCCEEEEEHEECCCCCEECCCCCCEEEEEEEECCCCCCC
GELTGHYVDWNEARQGGRYSDVIWDLHLRKEMHYGEKVSLEPFVSVRNLLNGHQYVYRVY
CCEEEEEECHHHHHCCCCCHHEEEEEEHHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEE
QNPGRWIELGVRCNF
CCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA