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The map label for this gene is purB [H]

Identifier: 197118714

GI number: 197118714

Start: 2707332

End: 2708627

Strand: Reverse

Name: purB [H]

Synonym: Gbem_2333

Alternate gene names: 197118714

Gene position: 2708627-2707332 (Counterclockwise)

Preceding gene: 197118715

Following gene: 197118713

Centisome position: 58.69

GC content: 60.8

Gene sequence:

>1296_bases
GTGATAGAACGTTACAGCCGTCCTGAAATGGCCCGTATCTGGGAACCTGAAAACCGCTACCGCAAGTGGCTCGAAATAGA
AATTTACGCCTGCGAGGCTCACGCCGAGATGGGACGCATACCGAAGGACGCCGTGGCCCGCATCAAGGCGAAAGCCAACT
TCGACGTACCCCGCATCGACGAGATCGAGCGCACCGTCAAGCACGACGTCATCGCTTTCCTCACCTCCGTCGCCGACTAC
ATCGGCGAAGATTCCCGTTTCGTCCACCTGGGGCTTACCTCTTCCGACGTCCTCGACACCTCCTTCGCCATGCTCCTGAA
GGAAGCGGGCGAGCTGATCATCGTCGACATCAAGCGTCTCATGGCCGTCATCAAGACCCGTGCCTACGAGCACAAGATGA
CGCCGCAGATGGGGCGCTCGCACGGCATCCACGCCGAGCCGGTCACCTTCGGCCTGAAGATGGCGCTTTGGTACGACGAG
ATGGCCAGGAACCTGAAGCGTATGGAAGCGGCCCTGGAAACCATCTCCTACGGCAAGCTCTCCGGCGCGGTCGGTACATT
CGCCAATATCGATCCGCAGGTCGAGGCTTACGTCTGCAAGAAGGCGGGATTGAAGCCCGCCCCCTGCTCCACGCAGGTGC
TGCAGCGCGACCGCCACGCCGAGTACTTCACCACCCTGGCGATCATCGCCTCCTCCATAGAGAAGTTCGCCGTCGAGATC
AGGCACCTGCAGCGCACCGAGGTGCTCGAAGCTGAGGAGTTCTTCAGCAAGGGGCAGAAAGGCTCCTCCGCGATGCCGCA
CAAGCGCAACCCGGTCCTCTCCGAAAACCTGACCGGCCTGGCGCGCCTGATCCGCGGCTATGCGGTCTCCGCCATGGAGA
ACGTCCCGCTGTGGCACGAGCGCGACATCTCGCACTCCTCGGTCGAGCGCATCATCGGCCCGGACGCAACCGTAATGCTC
GACTTCATGCTGAACCGCGCCATCGGCCTCATCGAGAACCTGGTGGTCTACCCCGAGAACATGATGCGCAACCTGAACCA
GATGCGCGGCCTCATCTTCTCGCAGCGCGTACTCTTAAAGCTCGCCGAGGCGGGCGCTTCCCGCGAGAAGGCCTATTCGC
TGGTGCAGAGAAACGCCATGAAGGTCTGGGAAGAGGGGAAAGACTTCCAGACCGAGCTTCTGAACGATGCCGATGTCGCC
GGGTTCCTTCCCGCCGAGGAGATCAAGGAAGCCTTCGATCTCGGTTACCATTTGAAACACGTCGACACTATTTTCACGAG
GGTCTTCGGTGGATAG

Upstream 100 bases:

>100_bases
GTTCTTTAAGCTTTGCAGTTCTTTATCTTTATCTTTATCTTTATCTCGATCTTAATCTCAATCTTAATCTTAATCTGTTT
ATTTAAGGGGGAAATGACTA

Downstream 100 bases:

>100_bases
CCTTTTGTACTACCTGACGCCCAACGAGATGGATCCCTTCTATCTCTTCTGGGCCAAGCAACTGTTCATATCGGTCGGTA
TCATCGCCGGCTTTTATCTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYSRPEMARIWEPENRYRKWLEIEIYACEAHAEMGRIPKDAVARIKAKANFDVPRIDEIERTVKHDVIAFLTSVADY
IGEDSRFVHLGLTSSDVLDTSFAMLLKEAGELIIVDIKRLMAVIKTRAYEHKMTPQMGRSHGIHAEPVTFGLKMALWYDE
MARNLKRMEAALETISYGKLSGAVGTFANIDPQVEAYVCKKAGLKPAPCSTQVLQRDRHAEYFTTLAIIASSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERIIGPDATVML
DFMLNRAIGLIENLVVYPENMMRNLNQMRGLIFSQRVLLKLAEAGASREKAYSLVQRNAMKVWEEGKDFQTELLNDADVA
GFLPAEEIKEAFDLGYHLKHVDTIFTRVFGG

Sequences:

>Translated_431_residues
MIERYSRPEMARIWEPENRYRKWLEIEIYACEAHAEMGRIPKDAVARIKAKANFDVPRIDEIERTVKHDVIAFLTSVADY
IGEDSRFVHLGLTSSDVLDTSFAMLLKEAGELIIVDIKRLMAVIKTRAYEHKMTPQMGRSHGIHAEPVTFGLKMALWYDE
MARNLKRMEAALETISYGKLSGAVGTFANIDPQVEAYVCKKAGLKPAPCSTQVLQRDRHAEYFTTLAIIASSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERIIGPDATVML
DFMLNRAIGLIENLVVYPENMMRNLNQMRGLIFSQRVLLKLAEAGASREKAYSLVQRNAMKVWEEGKDFQTELLNDADVA
GFLPAEEIKEAFDLGYHLKHVDTIFTRVFGG
>Mature_431_residues
MIERYSRPEMARIWEPENRYRKWLEIEIYACEAHAEMGRIPKDAVARIKAKANFDVPRIDEIERTVKHDVIAFLTSVADY
IGEDSRFVHLGLTSSDVLDTSFAMLLKEAGELIIVDIKRLMAVIKTRAYEHKMTPQMGRSHGIHAEPVTFGLKMALWYDE
MARNLKRMEAALETISYGKLSGAVGTFANIDPQVEAYVCKKAGLKPAPCSTQVLQRDRHAEYFTTLAIIASSIEKFAVEI
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERIIGPDATVML
DFMLNRAIGLIENLVVYPENMMRNLNQMRGLIFSQRVLLKLAEAGASREKAYSLVQRNAMKVWEEGKDFQTELLNDADVA
GFLPAEEIKEAFDLGYHLKHVDTIFTRVFGG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=29.064039408867, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1787376, Length=323, Percent_Identity=29.7213622291022, Blast_Score=101, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508577, Length=400, Percent_Identity=27.5, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=344, Percent_Identity=29.0697674418605, Blast_Score=108, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323391, Length=404, Percent_Identity=26.980198019802, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24647570, Length=403, Percent_Identity=28.287841191067, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48877; Mature: 48877

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPEMARIWEPENRYRKWLEIEIYACEAHAEMGRIPKDAVARIKAKANFDVPRID
CCCCCCCCCHHHHCCCHHHHHCEEEEEEEEEECHHHHCCCCHHHHHHHHHHCCCCCCCHH
EIERTVKHDVIAFLTSVADYIGEDSRFVHLGLTSSDVLDTSFAMLLKEAGELIIVDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHHHH
MAVIKTRAYEHKMTPQMGRSHGIHAEPVTFGLKMALWYDEMARNLKRMEAALETISYGKL
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPQVEAYVCKKAGLKPAPCSTQVLQRDRHAEYFTTLAIIASSIEKFAVEI
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERIIGPDATVMLDFMLNRAIGLIENLVVYPENMMRNLNQMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LAEAGASREKAYSLVQRNAMKVWEEGKDFQTELLNDADVAGFLPAEEIKEAFDLGYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHCCCCHHHHHHHHHHCHHHHH
VDTIFTRVFGG
HHHHHHHHHCC
>Mature Secondary Structure
MIERYSRPEMARIWEPENRYRKWLEIEIYACEAHAEMGRIPKDAVARIKAKANFDVPRID
CCCCCCCCCHHHHCCCHHHHHCEEEEEEEEEECHHHHCCCCHHHHHHHHHHCCCCCCCHH
EIERTVKHDVIAFLTSVADYIGEDSRFVHLGLTSSDVLDTSFAMLLKEAGELIIVDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEHHHH
MAVIKTRAYEHKMTPQMGRSHGIHAEPVTFGLKMALWYDEMARNLKRMEAALETISYGKL
HHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPQVEAYVCKKAGLKPAPCSTQVLQRDRHAEYFTTLAIIASSIEKFAVEI
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEFFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERIIGPDATVMLDFMLNRAIGLIENLVVYPENMMRNLNQMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LAEAGASREKAYSLVQRNAMKVWEEGKDFQTELLNDADVAGFLPAEEIKEAFDLGYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHCCCCHHHHHHHHHHCHHHHH
VDTIFTRVFGG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]