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The map label for this gene is fusA-1 [H]

Identifier: 197118699

GI number: 197118699

Start: 2690374

End: 2692479

Strand: Reverse

Name: fusA-1 [H]

Synonym: Gbem_2318

Alternate gene names: 197118699

Gene position: 2692479-2690374 (Counterclockwise)

Preceding gene: 197118700

Following gene: 197118698

Centisome position: 58.34

GC content: 61.92

Gene sequence:

>2106_bases
ATGGGAAGGTATGAAACGGCAAAACTGCGGAACCTCGGAATTGTAGCCCACGGCGGTGCGGGCAAGACCTCGCTCGCCGA
GGCCATCCTGTTCGACACAGGCATGATCGACCGACTGGGCCGCGTCGACGACGGCACCTCCACCATGGATTACGAGCCCG
AGGAGGTCAAACGACGCATCTCCATCACCTCCTCCCTGGACCACTGCGAGTGGCACGGGTGCTCCATCCATCTGGTCGAC
ACCCCGGGCTACGGCAACTTCATCGCCGACACCCGCGCCTGCATGAGAACCCTCGACTGCGCCGTCGTGATCCTCTCCGC
CATCTCCGGCGTCAAGGTCCAGACCGAGGAGGTCTGGCAATGGGCCAATGAGTTCGAGATCCCGCGCATCGCCTTCGTCA
ACAAGATGGACCGTGAGAGGGCCAACTTCTTCCGCGCCATCGATGATATGGAGAAGGCGCTGGGGGCGCGCGGCGTGGCG
GTGCAGATACCGCTTGGGTCCGAGGAGAACTTCGAGGGGGTCATCGACCTGGTGCGCATGAAGGCGTACCGGTATCAAAA
AGACGGGTCGGGGAAGTTCAGCGAGGGGGAGATCCCTGCCGACGACCTTGCCGAGGCGCAGCGCCTCAGGGACCTGATGG
TCGAGACGGTGGCGGAGGCGTACGACGCGCTCACGGAGAAGTTCCTGGATACTGGGGAACTGACCGAGGAAGAGATCCTG
GACGGGCTCAGGGTCGGTACCCTGAGAAGCACCTTTACCGCCGTCTTCTGCGGTTCGGCTACGGCAAACATAGGGATCGC
CCAGCTTCTGGACGGGATCTGCGCTTACCTCCCGTCCCCCTTGGACCGCACCAAGGCGGTGGGAATCGATCCCAAGACCG
AGCAGGTCATCGAGCGCGCCCCCTCGGAGAGCGAGCCGTTATCCGCGCTGGTGTTCAAGACCACCTCCGACCCCTACACC
GGGAAGATCACCATCTTCAGGGTCTATTCCGGGGTGCTCAACTCCGATTCGCTGGTGTACAACTCCACCAAGGAAGTGCA
GGAGAGGATCGGACAGATCTACGAGCTTGAGGGTAAAAAGCAGCACCCGATCAAGCAGGCCGTGGCGGGCGACATCGTCG
CGGTGGCGAAGCTCAAAGAGACGGTCACCGGGGACACGCTCTGCGACGAGACGAAGCCGATCGTCTACGAGCCGGCCCAG
CCGCTCCAGCCTGTCATCTCCTACGCCATCGAGCCCAAGACCAAGGCCGACGAGGACAAGATCCACTCGGCCCTGCAGCG
CATGATCGAGGAGGATCCGACCATAGAGTCGCACCGCGACCCTCAGACCCGCGAGTTCATCATTTCGGGGATGGGGCAGG
TGCACCTCGAGGTGATAGTCGAGAAGATGAAGCGTAAGTTCGGCGCGGACGTCCTCTTGAAAACCCCCAAGGTCCCGTAC
CTGGAGACCATCCGCGGCAGCGCCAAGGTGCAGGGGAAATACAAGAAGCAGTCCGGCGGCCGCGGCCAGTACGGCGATTG
CTGGATCGAACTCTCCCCGTTGCCGCGCGGCGAGGGGTACCTGTTCGAGGACAAGATCGTCGGCGGCGTCATTCCGCGCC
AGTACATCCCGGCGGTAGACAAGGGGATCCAGGAGGCGGCCAAGGAAGGCTTCCTCGCCGGAAACCCTGTGGTGGACTTC
AAGGTGGCTCTCTACGACGGCTCGTTCCATACCGTCGACTCGTCCGAGATGGCCTTCAAGGTGGCGGGTTCCATGGCCTT
CAAGAAGGCGATGGAACAGTGCAAGCCGGTCCTTTTGGAGCCGATCGTCAACATGAAGATTACGGTCCCCGACGAGAACA
TGGGGGACGTCATCGGCGATCTCAACTCCCGTCGCGGCAAGGTAGTCGGTGTCGAGCCCAAGGCGAACTCGCAGATCATC
AGGAGCGTGGTCCCCATGTCCGAGGTGCTCACCTACTCGAACGACCTCCGTTCCATGACCAGCGACCGCGGCATGTTCAC
CTCCGAGTTCTCTCACTATGAGGAGGTCCCGAGCCACCTGGCGCAGAAGATCATCCAGGAGGCGCTGGATCAAAAGGGTA
ACGGCAACAACCATAAAAGCGCTTAG

Upstream 100 bases:

>100_bases
TACCGCCGGGTAGTTTGTTGTTGGTTTCGTCTGCTTTACTCAAGTCAGATCTCGTTTCAGAGTAGGTCATCACTGATTTT
CTATTCTAAGGGAGGGCACT

Downstream 100 bases:

>100_bases
TTTGCGCTTAGCATAACGTGCCCGGTCGCTTCGAGACCGGGCACCGCCTTAATTCAACAGCAGATGCAAAAAGGGAGGAT
GAGCTAGATGCAGAATCAAT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MGRYETAKLRNLGIVAHGGAGKTSLAEAILFDTGMIDRLGRVDDGTSTMDYEPEEVKRRISITSSLDHCEWHGCSIHLVD
TPGYGNFIADTRACMRTLDCAVVILSAISGVKVQTEEVWQWANEFEIPRIAFVNKMDRERANFFRAIDDMEKALGARGVA
VQIPLGSEENFEGVIDLVRMKAYRYQKDGSGKFSEGEIPADDLAEAQRLRDLMVETVAEAYDALTEKFLDTGELTEEEIL
DGLRVGTLRSTFTAVFCGSATANIGIAQLLDGICAYLPSPLDRTKAVGIDPKTEQVIERAPSESEPLSALVFKTTSDPYT
GKITIFRVYSGVLNSDSLVYNSTKEVQERIGQIYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCDETKPIVYEPAQ
PLQPVISYAIEPKTKADEDKIHSALQRMIEEDPTIESHRDPQTREFIISGMGQVHLEVIVEKMKRKFGADVLLKTPKVPY
LETIRGSAKVQGKYKKQSGGRGQYGDCWIELSPLPRGEGYLFEDKIVGGVIPRQYIPAVDKGIQEAAKEGFLAGNPVVDF
KVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEQCKPVLLEPIVNMKITVPDENMGDVIGDLNSRRGKVVGVEPKANSQII
RSVVPMSEVLTYSNDLRSMTSDRGMFTSEFSHYEEVPSHLAQKIIQEALDQKGNGNNHKSA

Sequences:

>Translated_701_residues
MGRYETAKLRNLGIVAHGGAGKTSLAEAILFDTGMIDRLGRVDDGTSTMDYEPEEVKRRISITSSLDHCEWHGCSIHLVD
TPGYGNFIADTRACMRTLDCAVVILSAISGVKVQTEEVWQWANEFEIPRIAFVNKMDRERANFFRAIDDMEKALGARGVA
VQIPLGSEENFEGVIDLVRMKAYRYQKDGSGKFSEGEIPADDLAEAQRLRDLMVETVAEAYDALTEKFLDTGELTEEEIL
DGLRVGTLRSTFTAVFCGSATANIGIAQLLDGICAYLPSPLDRTKAVGIDPKTEQVIERAPSESEPLSALVFKTTSDPYT
GKITIFRVYSGVLNSDSLVYNSTKEVQERIGQIYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCDETKPIVYEPAQ
PLQPVISYAIEPKTKADEDKIHSALQRMIEEDPTIESHRDPQTREFIISGMGQVHLEVIVEKMKRKFGADVLLKTPKVPY
LETIRGSAKVQGKYKKQSGGRGQYGDCWIELSPLPRGEGYLFEDKIVGGVIPRQYIPAVDKGIQEAAKEGFLAGNPVVDF
KVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEQCKPVLLEPIVNMKITVPDENMGDVIGDLNSRRGKVVGVEPKANSQII
RSVVPMSEVLTYSNDLRSMTSDRGMFTSEFSHYEEVPSHLAQKIIQEALDQKGNGNNHKSA
>Mature_700_residues
GRYETAKLRNLGIVAHGGAGKTSLAEAILFDTGMIDRLGRVDDGTSTMDYEPEEVKRRISITSSLDHCEWHGCSIHLVDT
PGYGNFIADTRACMRTLDCAVVILSAISGVKVQTEEVWQWANEFEIPRIAFVNKMDRERANFFRAIDDMEKALGARGVAV
QIPLGSEENFEGVIDLVRMKAYRYQKDGSGKFSEGEIPADDLAEAQRLRDLMVETVAEAYDALTEKFLDTGELTEEEILD
GLRVGTLRSTFTAVFCGSATANIGIAQLLDGICAYLPSPLDRTKAVGIDPKTEQVIERAPSESEPLSALVFKTTSDPYTG
KITIFRVYSGVLNSDSLVYNSTKEVQERIGQIYELEGKKQHPIKQAVAGDIVAVAKLKETVTGDTLCDETKPIVYEPAQP
LQPVISYAIEPKTKADEDKIHSALQRMIEEDPTIESHRDPQTREFIISGMGQVHLEVIVEKMKRKFGADVLLKTPKVPYL
ETIRGSAKVQGKYKKQSGGRGQYGDCWIELSPLPRGEGYLFEDKIVGGVIPRQYIPAVDKGIQEAAKEGFLAGNPVVDFK
VALYDGSFHTVDSSEMAFKVAGSMAFKKAMEQCKPVLLEPIVNMKITVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIR
SVVPMSEVLTYSNDLRSMTSDRGMFTSEFSHYEEVPSHLAQKIIQEALDQKGNGNNHKSA

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=698, Percent_Identity=33.3810888252149, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI19923640, Length=708, Percent_Identity=29.6610169491525, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI25306283, Length=392, Percent_Identity=34.4387755102041, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI25306287, Length=288, Percent_Identity=37.5, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI4503483, Length=855, Percent_Identity=21.4035087719298, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI157426893, Length=216, Percent_Identity=26.3888888888889, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1789738, Length=693, Percent_Identity=46.7532467532467, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1790835, Length=507, Percent_Identity=24.4575936883629, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI48994988, Length=131, Percent_Identity=32.0610687022901, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17533571, Length=696, Percent_Identity=32.9022988505747, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17556745, Length=722, Percent_Identity=25.0692520775623, Blast_Score=234, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6323098, Length=690, Percent_Identity=35.0724637681159, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322359, Length=798, Percent_Identity=27.8195488721804, Blast_Score=294, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=34.0028694404591, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI221458488, Length=733, Percent_Identity=27.4215552523874, Blast_Score=253, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24585711, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 77327; Mature: 77196

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYETAKLRNLGIVAHGGAGKTSLAEAILFDTGMIDRLGRVDDGTSTMDYEPEEVKRRI
CCCCCCCHHHCCCEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHH
SITSSLDHCEWHGCSIHLVDTPGYGNFIADTRACMRTLDCAVVILSAISGVKVQTEEVWQ
HHHCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
WANEFEIPRIAFVNKMDRERANFFRAIDDMEKALGARGVAVQIPLGSEENFEGVIDLVRM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
KAYRYQKDGSGKFSEGEIPADDLAEAQRLRDLMVETVAEAYDALTEKFLDTGELTEEEIL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DGLRVGTLRSTFTAVFCGSATANIGIAQLLDGICAYLPSPLDRTKAVGIDPKTEQVIERA
CCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHC
PSESEPLSALVFKTTSDPYTGKITIFRVYSGVLNSDSLVYNSTKEVQERIGQIYELEGKK
CCCCCCCEEEEEEECCCCCCCCEEEEEEEHHHCCCCCEEECCHHHHHHHHHHHEECCCCC
QHPIKQAVAGDIVAVAKLKETVTGDTLCDETKPIVYEPAQPLQPVISYAIEPKTKADEDK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCHHH
IHSALQRMIEEDPTIESHRDPQTREFIISGMGQVHLEVIVEKMKRKFGADVLLKTPKVPY
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCH
LETIRGSAKVQGKYKKQSGGRGQYGDCWIELSPLPRGEGYLFEDKIVGGVIPRQYIPAVD
HHHHCCCCEECCCHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCHHCCCCCHHHHHHHH
KGIQEAAKEGFLAGNPVVDFKVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEQCKPVLLE
HHHHHHHHCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PIVNMKITVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIRSVVPMSEVLTYSNDLRSMT
HHCCCEEECCCCCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGMFTSEFSHYEEVPSHLAQKIIQEALDQKGNGNNHKSA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GRYETAKLRNLGIVAHGGAGKTSLAEAILFDTGMIDRLGRVDDGTSTMDYEPEEVKRRI
CCCCCCHHHCCCEEEECCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHH
SITSSLDHCEWHGCSIHLVDTPGYGNFIADTRACMRTLDCAVVILSAISGVKVQTEEVWQ
HHHCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
WANEFEIPRIAFVNKMDRERANFFRAIDDMEKALGARGVAVQIPLGSEENFEGVIDLVRM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHH
KAYRYQKDGSGKFSEGEIPADDLAEAQRLRDLMVETVAEAYDALTEKFLDTGELTEEEIL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
DGLRVGTLRSTFTAVFCGSATANIGIAQLLDGICAYLPSPLDRTKAVGIDPKTEQVIERA
CCHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHC
PSESEPLSALVFKTTSDPYTGKITIFRVYSGVLNSDSLVYNSTKEVQERIGQIYELEGKK
CCCCCCCEEEEEEECCCCCCCCEEEEEEEHHHCCCCCEEECCHHHHHHHHHHHEECCCCC
QHPIKQAVAGDIVAVAKLKETVTGDTLCDETKPIVYEPAQPLQPVISYAIEPKTKADEDK
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCCCHHH
IHSALQRMIEEDPTIESHRDPQTREFIISGMGQVHLEVIVEKMKRKFGADVLLKTPKVPY
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCH
LETIRGSAKVQGKYKKQSGGRGQYGDCWIELSPLPRGEGYLFEDKIVGGVIPRQYIPAVD
HHHHCCCCEECCCHHHCCCCCCCCCCEEEEECCCCCCCCEEECCCHHCCCCCHHHHHHHH
KGIQEAAKEGFLAGNPVVDFKVALYDGSFHTVDSSEMAFKVAGSMAFKKAMEQCKPVLLE
HHHHHHHHCCCCCCCCEEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PIVNMKITVPDENMGDVIGDLNSRRGKVVGVEPKANSQIIRSVVPMSEVLTYSNDLRSMT
HHCCCEEECCCCCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SDRGMFTSEFSHYEEVPSHLAQKIIQEALDQKGNGNNHKSA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA