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The map label for this gene is gltX

Identifier: 197118660

GI number: 197118660

Start: 2649687

End: 2651090

Strand: Reverse

Name: gltX

Synonym: Gbem_2279

Alternate gene names: 197118660

Gene position: 2651090-2649687 (Counterclockwise)

Preceding gene: 197118661

Following gene: 197118658

Centisome position: 57.44

GC content: 61.82

Gene sequence:

>1404_bases
ATGTCACAAGTACGCCTCCGTTTCGCACCCAGCCCGACCGGGTACCTCCACGTGGGGGGAGCGCGCACCGCGCTTTTTAA
CTGGCTTTTGGCGAGGAAGCAGCAGGGGAAGTTCATCCTCAGGATCGAGGACACCGACGTCGCCCGCTCCACCCAGGAGT
CGGTCGACGCCATCCTGGAAGGGATGACCTGGCTCGGACTGGACTGGGACGAGGGGCCTTTCTACCAGTCTGACAACTTT
CCTCTCTACAAGGAGTACGTCGAGAAGCTGATCGCCGCCGGGAAAGCGTACAAGTGCTACTGCAGCGCCGAAGAACTGGA
GGTCAAGCGCGAGAAGGCGCTCAAGGAAGGTGGCAAGCCCAAGTACGACGGGACCTGCCGCAACCTGCCGCAGGGCGCGG
ACGACGGGCGCCCCTACGTGGTCCGTTTTAAGGCGCCGCAGGAAGGGACCACCTACTGGAACGACCTGATCAAGGGGAAG
ATATCCTTTGAGAACGCCGAACTGGACGACCTGATCATCCAGCGCACCGACGGGACCCCCACCTACAACTTCGTGGTGGT
AATCGACGACGCCACCATGGGGGTCACCACCGTCATCCGCGGCGACGACCACGTGAACAACACGCCGCGCCAGATCCTTC
TGTACGAGGCCCTGGAGGTGCCGGTTCCCCAGTTCGCCCACGTCCCGATGATCCTTGGGGCGGACAAGGCGAGGCTCTCG
AAAAGGCACGGCGCCACTTCGGTCATGGCCTACCGGGACATGGGCTTTCTTCCCGAGGCGATGGTCAACTACCTGGTCCG
CCTGGGCTGGAGTTACGGCGACGAGGAGATCTTCTCGCTCGAAGATCTGGTCCAGAAGTTCTCCATCGAGAACGTGGGTC
GGTCCGCGGGCGTTTTCAACCCGGACAAGCTCCTGTGGTTGAACGCGCACTACATCAAAAACGGCGACCCTGTGCGACTG
GCCGGGCTCCTGATTCCTTTCCTGAAGGAGCGGGGGGTAACCGATACCGCAGGTGGACCTGAGCTTCCGGCCGTGGTGAA
GACACTGCAGGAAAGGGCTAAGACCATGCTGGAATTGGCTGACGGAGCGCTGTTCTACTACCAGCAGGAGCTCTCCTACG
ACGAGAAGGGGGTAGCCAAGTTCTTCAACCCCGAGACCCCGGCGCTTCTTAGGGGGCTGTTCAAGAAATTGGGCGCGGTG
GGTGAGCTCACTACCGTCGCAATAGAAGGCGTTTTCAAGGAGCTTTGCGAGGAGAAGGGGATCAAGCTCGGGCAGGTAGG
GCCCGCGGTGAGGCTAGCCCTGTCGGGCTCCACCGCTTCGCCGGGGATCTACGAGATGATCGAGGTGCTGGGGCTGGAAG
AGACCGGGAAGCGCCTGGAGCGGGCCATAGCCAAGCTCTGCTGA

Upstream 100 bases:

>100_bases
AATCTGGGGACGAGGAGCCCGGGGACGAGTAGTCCAGGCATTTGCAGCAAACACATTTTTTGCAGCCATGCAATGATCAC
AGCAAAGGAAGGAGCTTTTC

Downstream 100 bases:

>100_bases
TAACGGCAGCAGGTAAAAGAAAGAGGGGAAGCTCCAGGGCTTCCCCTCTTTTTCGTGGTGCGGATGTAGAGGGAGGTTCC
GCCCCTAACGCTTTTTGACT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSQVRLRFAPSPTGYLHVGGARTALFNWLLARKQQGKFILRIEDTDVARSTQESVDAILEGMTWLGLDWDEGPFYQSDNF
PLYKEYVEKLIAAGKAYKCYCSAEELEVKREKALKEGGKPKYDGTCRNLPQGADDGRPYVVRFKAPQEGTTYWNDLIKGK
ISFENAELDDLIIQRTDGTPTYNFVVVIDDATMGVTTVIRGDDHVNNTPRQILLYEALEVPVPQFAHVPMILGADKARLS
KRHGATSVMAYRDMGFLPEAMVNYLVRLGWSYGDEEIFSLEDLVQKFSIENVGRSAGVFNPDKLLWLNAHYIKNGDPVRL
AGLLIPFLKERGVTDTAGGPELPAVVKTLQERAKTMLELADGALFYYQQELSYDEKGVAKFFNPETPALLRGLFKKLGAV
GELTTVAIEGVFKELCEEKGIKLGQVGPAVRLALSGSTASPGIYEMIEVLGLEETGKRLERAIAKLC

Sequences:

>Translated_467_residues
MSQVRLRFAPSPTGYLHVGGARTALFNWLLARKQQGKFILRIEDTDVARSTQESVDAILEGMTWLGLDWDEGPFYQSDNF
PLYKEYVEKLIAAGKAYKCYCSAEELEVKREKALKEGGKPKYDGTCRNLPQGADDGRPYVVRFKAPQEGTTYWNDLIKGK
ISFENAELDDLIIQRTDGTPTYNFVVVIDDATMGVTTVIRGDDHVNNTPRQILLYEALEVPVPQFAHVPMILGADKARLS
KRHGATSVMAYRDMGFLPEAMVNYLVRLGWSYGDEEIFSLEDLVQKFSIENVGRSAGVFNPDKLLWLNAHYIKNGDPVRL
AGLLIPFLKERGVTDTAGGPELPAVVKTLQERAKTMLELADGALFYYQQELSYDEKGVAKFFNPETPALLRGLFKKLGAV
GELTTVAIEGVFKELCEEKGIKLGQVGPAVRLALSGSTASPGIYEMIEVLGLEETGKRLERAIAKLC
>Mature_466_residues
SQVRLRFAPSPTGYLHVGGARTALFNWLLARKQQGKFILRIEDTDVARSTQESVDAILEGMTWLGLDWDEGPFYQSDNFP
LYKEYVEKLIAAGKAYKCYCSAEELEVKREKALKEGGKPKYDGTCRNLPQGADDGRPYVVRFKAPQEGTTYWNDLIKGKI
SFENAELDDLIIQRTDGTPTYNFVVVIDDATMGVTTVIRGDDHVNNTPRQILLYEALEVPVPQFAHVPMILGADKARLSK
RHGATSVMAYRDMGFLPEAMVNYLVRLGWSYGDEEIFSLEDLVQKFSIENVGRSAGVFNPDKLLWLNAHYIKNGDPVRLA
GLLIPFLKERGVTDTAGGPELPAVVKTLQERAKTMLELADGALFYYQQELSYDEKGVAKFFNPETPALLRGLFKKLGAVG
ELTTVAIEGVFKELCEEKGIKLGQVGPAVRLALSGSTASPGIYEMIEVLGLEETGKRLERAIAKLC

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=490, Percent_Identity=31.4285714285714, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI62241042, Length=400, Percent_Identity=26.75, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=48.3731019522777, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=37.4485596707819, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786895, Length=341, Percent_Identity=22.8739002932551, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=30.625, Blast_Score=231, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6324540, Length=485, Percent_Identity=33.4020618556701, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321192, Length=260, Percent_Identity=30, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324742, Length=213, Percent_Identity=30.5164319248826, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=33.1288343558282, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24649466, Length=242, Percent_Identity=28.5123966942149, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864488, Length=311, Percent_Identity=25.08038585209, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_GEOBB (B5EEE6)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139087.1
- GeneID:   6782275
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2279
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51922; Mature: 51791

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVRLRFAPSPTGYLHVGGARTALFNWLLARKQQGKFILRIEDTDVARSTQESVDAILE
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHC
GMTWLGLDWDEGPFYQSDNFPLYKEYVEKLIAAGKAYKCYCSAEELEVKREKALKEGGKP
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCC
KYDGTCRNLPQGADDGRPYVVRFKAPQEGTTYWNDLIKGKISFENAELDDLIIQRTDGTP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCCCEEEEECCCCC
TYNFVVVIDDATMGVTTVIRGDDHVNNTPRQILLYEALEVPVPQFAHVPMILGADKARLS
CEEEEEEECCCCCCEEEEEECCCCCCCCCHHEEEHHHHCCCCCCCCCCCEEECCCHHHHH
KRHGATSVMAYRDMGFLPEAMVNYLVRLGWSYGDEEIFSLEDLVQKFSIENVGRSAGVFN
HHCCCCCEEHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PDKLLWLNAHYIKNGDPVRLAGLLIPFLKERGVTDTAGGPELPAVVKTLQERAKTMLELA
CCEEEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DGALFYYQQELSYDEKGVAKFFNPETPALLRGLFKKLGAVGELTTVAIEGVFKELCEEKG
CCEEEEEHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IKLGQVGPAVRLALSGSTASPGIYEMIEVLGLEETGKRLERAIAKLC
CEECCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SQVRLRFAPSPTGYLHVGGARTALFNWLLARKQQGKFILRIEDTDVARSTQESVDAILE
CCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHC
GMTWLGLDWDEGPFYQSDNFPLYKEYVEKLIAAGKAYKCYCSAEELEVKREKALKEGGKP
CCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCC
KYDGTCRNLPQGADDGRPYVVRFKAPQEGTTYWNDLIKGKISFENAELDDLIIQRTDGTP
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCEEEECCCCCCCEEEEECCCCC
TYNFVVVIDDATMGVTTVIRGDDHVNNTPRQILLYEALEVPVPQFAHVPMILGADKARLS
CEEEEEEECCCCCCEEEEEECCCCCCCCCHHEEEHHHHCCCCCCCCCCCEEECCCHHHHH
KRHGATSVMAYRDMGFLPEAMVNYLVRLGWSYGDEEIFSLEDLVQKFSIENVGRSAGVFN
HHCCCCCEEHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PDKLLWLNAHYIKNGDPVRLAGLLIPFLKERGVTDTAGGPELPAVVKTLQERAKTMLELA
CCEEEEEEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DGALFYYQQELSYDEKGVAKFFNPETPALLRGLFKKLGAVGELTTVAIEGVFKELCEEKG
CCEEEEEHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
IKLGQVGPAVRLALSGSTASPGIYEMIEVLGLEETGKRLERAIAKLC
CEECCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA