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The map label for this gene is fdnH-1 [C]

Identifier: 197118647

GI number: 197118647

Start: 2634920

End: 2635759

Strand: Reverse

Name: fdnH-1 [C]

Synonym: Gbem_2266

Alternate gene names: 197118647

Gene position: 2635759-2634920 (Counterclockwise)

Preceding gene: 308535295

Following gene: 197118646

Centisome position: 57.11

GC content: 60.95

Gene sequence:

>840_bases
ATGAGCGAGACCAACCAGGATTTCAACAAGAGCAAGGCGTTCTTGATCGACATGACCAAGTGCACCGGCTGCCGCGGCTG
CCAGGTCGCCTGCAAGCAATGGAACCAGCTCGACGCCGAGAAGACGGAGTTCTTCAACGGCGAGGGTTACCAGAACCCGC
CGCTTATGTCGGAGCACACCTTTACCCGCATCAAGTTCCGCGACTACGAGAAGAACGGGCAGAACGAGTTCGCCTTCTAC
AAAGAGATGTGCATGCACTGCAACGAGCCGGCGTGCGCCTCCGTCTGCCCGGTCGGCGCCTTCAAGAAGACCAAGGAAGG
CCCCGTCGTCTACGACGCCAAGCGCTGCATCGGCTGCCGTTTCTGCATGGTCGCCTGCCCGTTCGGGGTTCCCAAGTACG
AGTGGAGCAAGGCATTTCCGCTGGTGAGAAAATGCACCGGCTGCTACTCCAGGATCAAGGAAGGGCTGAAGCCCGCCTGC
GCCACCACCTGCGTCTCCGCCATCACCTACGGCGACCGCGGGGAGATGATCAAGGAGGCGAACAAGCGCATCGCCACCCG
CCCCGAGAAGTACCTGAAGAAGCTCTACGGAGCCGAAGAGGCGGGTGGGACCAGCGTCATCTACCTGACCGCGCTCCCCT
TCGACGAGCTAGGGTTCAAACCGGTCACCAAGAGGCCGCTCCCGTCCTACACCTGGCAGGCGCTGCGCCTCGTGCCGGGG
ATCTTCCTGACTGTCGGCAGTTCGCTCTCGCTCCTTTCCTGGTTCAACCACAGGAAAGAAAGGATCGCCAAGGAAGAGGA
ACAGAGAAAATCCGGCGCAACGCCTAAGGAGGAGAGCTAG

Upstream 100 bases:

>100_bases
ACTCGGGCAACGTCCTGACCCCGTCCGTCGGCTGCGCCAATACGAGCATCCCCGAATTCAAGGCATTCCTCTGCAACATC
GAGAAAGGGGGTAAACGCGC

Downstream 100 bases:

>100_bases
ATGACCGCTGCAAAGATAATCATCAACGAGATCAAGGGATACCACCGCTTCGTGAAATTCCTGATCGTCCTGGTATCGCT
GGGCGCGCTAGCCTCCCTGG

Product: formate dehydrogenase iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSETNQDFNKSKAFLIDMTKCTGCRGCQVACKQWNQLDAEKTEFFNGEGYQNPPLMSEHTFTRIKFRDYEKNGQNEFAFY
KEMCMHCNEPACASVCPVGAFKKTKEGPVVYDAKRCIGCRFCMVACPFGVPKYEWSKAFPLVRKCTGCYSRIKEGLKPAC
ATTCVSAITYGDRGEMIKEANKRIATRPEKYLKKLYGAEEAGGTSVIYLTALPFDELGFKPVTKRPLPSYTWQALRLVPG
IFLTVGSSLSLLSWFNHRKERIAKEEEQRKSGATPKEES

Sequences:

>Translated_279_residues
MSETNQDFNKSKAFLIDMTKCTGCRGCQVACKQWNQLDAEKTEFFNGEGYQNPPLMSEHTFTRIKFRDYEKNGQNEFAFY
KEMCMHCNEPACASVCPVGAFKKTKEGPVVYDAKRCIGCRFCMVACPFGVPKYEWSKAFPLVRKCTGCYSRIKEGLKPAC
ATTCVSAITYGDRGEMIKEANKRIATRPEKYLKKLYGAEEAGGTSVIYLTALPFDELGFKPVTKRPLPSYTWQALRLVPG
IFLTVGSSLSLLSWFNHRKERIAKEEEQRKSGATPKEES
>Mature_278_residues
SETNQDFNKSKAFLIDMTKCTGCRGCQVACKQWNQLDAEKTEFFNGEGYQNPPLMSEHTFTRIKFRDYEKNGQNEFAFYK
EMCMHCNEPACASVCPVGAFKKTKEGPVVYDAKRCIGCRFCMVACPFGVPKYEWSKAFPLVRKCTGCYSRIKEGLKPACA
TTCVSAITYGDRGEMIKEANKRIATRPEKYLKKLYGAEEAGGTSVIYLTALPFDELGFKPVTKRPLPSYTWQALRLVPGI
FLTVGSSLSLLSWFNHRKERIAKEEEQRKSGATPKEES

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=208, Percent_Identity=36.5384615384615, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789370, Length=248, Percent_Identity=35.4838709677419, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1790326, Length=208, Percent_Identity=37.0192307692308, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787122, Length=185, Percent_Identity=37.8378378378378, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787872, Length=185, Percent_Identity=37.2972972972973, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI226510944, Length=173, Percent_Identity=27.1676300578035, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787478, Length=117, Percent_Identity=32.4786324786325, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787740, Length=111, Percent_Identity=34.2342342342342, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367245, Length=151, Percent_Identity=29.8013245033113, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367345, Length=192, Percent_Identity=25.5208333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787963, Length=123, Percent_Identity=30.8943089430894, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789079, Length=128, Percent_Identity=28.125, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 31553; Mature: 31421

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETNQDFNKSKAFLIDMTKCTGCRGCQVACKQWNQLDAEKTEFFNGEGYQNPPLMSEHT
CCCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCC
FTRIKFRDYEKNGQNEFAFYKEMCMHCNEPACASVCPVGAFKKTKEGPVVYDAKRCIGCR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHCCCCCCEEEEHHHHHHHH
FCMVACPFGVPKYEWSKAFPLVRKCTGCYSRIKEGLKPACATTCVSAITYGDRGEMIKEA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
NKRIATRPEKYLKKLYGAEEAGGTSVIYLTALPFDELGFKPVTKRPLPSYTWQALRLVPG
HHHHCCCHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHH
IFLTVGSSLSLLSWFNHRKERIAKEEEQRKSGATPKEES
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SETNQDFNKSKAFLIDMTKCTGCRGCQVACKQWNQLDAEKTEFFNGEGYQNPPLMSEHT
CCCCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCC
FTRIKFRDYEKNGQNEFAFYKEMCMHCNEPACASVCPVGAFKKTKEGPVVYDAKRCIGCR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCHHCCCCCCEEEEHHHHHHHH
FCMVACPFGVPKYEWSKAFPLVRKCTGCYSRIKEGLKPACATTCVSAITYGDRGEMIKEA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
NKRIATRPEKYLKKLYGAEEAGGTSVIYLTALPFDELGFKPVTKRPLPSYTWQALRLVPG
HHHHCCCHHHHHHHHHCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHH
IFLTVGSSLSLLSWFNHRKERIAKEEEQRKSGATPKEES
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]