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The map label for this gene is leuC1 [H]

Identifier: 197118639

GI number: 197118639

Start: 2626739

End: 2628685

Strand: Reverse

Name: leuC1 [H]

Synonym: Gbem_2258

Alternate gene names: 197118639

Gene position: 2628685-2626739 (Counterclockwise)

Preceding gene: 197118640

Following gene: 197118638

Centisome position: 56.96

GC content: 63.23

Gene sequence:

>1947_bases
ATGGGCGACAATGTGGCGATGAAAATATTGAAAGAGCACCTGGTCGAAGGGAAGCTGGTGCCGGGGCAGGAAATAGCCTT
GAGGATCGACCAGACGCTGTTGCAGGACGCGACGGGGACCATGGCGATGCTGGAGTTCATGGCGATGGGTCTTCCGCGGG
TGAAGGTGGAACTGGCGGCGCAGTACGTCGATCACAATCTGCTGCAGACCGACAACAAGAACGCCGACGACCACGCCTTT
TTAACCAGCGCGGCCCAAAAGTTCGGCGTCTACCTCTCCAAGCCGGGGAACGGGGTCTCGCACCAGGTCTCCCTGGAGCG
CTTCGGCGTCCCTGGCACCACCCTTTTGGGCGCGGACTCGCATACGCCTACCGCGGCTGGCCTTGGTCAACTCGCCATCG
GCGCCGGGGGGCTGGATGTCGCGCTCGCCATGGCGGGGCACCCCTTTCATCTCACTTGCCCACGCATCTGGGGGGTAAAG
CTGACCGGGCAGCTGCAACCCTGGGTCTCAGGGAAGGACGTGATCCTGGAGATGCTAAGGCGCCACAGCGTGAAGGGAGG
GGTGGGGAAGATCATCGAGTACTACGGCCCAGGGGTCGCCACCCTTTCCGCCACCGACCGCGCCACCATCGGCAACATGG
GGGCCGAGCTTGGCGCAACCAGCACCGTTTTCCCGAGCGACCAACACACACTTGCCTTTCTTAAGGCCCAGGGGCGCGGC
GAGGTGTGGCGCGAACTATTGCCGGACCCGGACGCGACCTACGACGACCACGACGAGATAGACCTGAGCCAGGTGGTCCC
GCTCATCGCCTGCCCTTCGTCGCCGGACAACGTGGTGCCGGTCTCGGAGATCGAGGGGTTGAAGGTGGACCAGGTCATCG
TCGGCAGTTCGGTCAACTCCTCTTTCCGCGACCTGATGACCGTCTGCCGCATCCTCGACGGGCGCAAGGTTGCGCCGGGC
GTCGCCTTCCATATCAACCCCGGCAGCCGACAGGTGCTGGAGAACGTGGTGGCGCAGGGGGGATTCATCATGCTCCTTCT
GGCCGGAGGCCAGGTGCACCAGCCCGGCTGCCTGGGCTGCATCGGCATGGGGCAGGCCCCCGGAACCAACCAGGTGTCGC
TTCGGACCTTCCCGCGCAATTTCCCTGGGCGAAGCGGCACCAAGGAGGACCGGGTCTACCTCTGCTCCCCCGAGACCGCT
GCTGCGGCCGGGATATTCGGGGTGGTTACCGATCCGCGCAAACTTTCCGACCTCCTCTTCTGGCCCGCGGTGCAGAACCC
CGAGCAGTACCTGGTGGACGACGCCGGCATTATCGTGCCCCCCGAGGGCGGCAGCGCCGTGGAGATCGTCACCGGCCCCA
ACATCGTTCCTTTCCCGGAGTTCCCCGCTCTGCCGGACCAGCTCGATGTCGAGGTGATCATCAAGGTCGGCGACAACATC
TCCACCGACTCCATCATGCCGGCAGGAAACAAGGTGCTCCCGTTCAGGAGCAACATCCCCGCCATCAGCAAGTTTGTCTT
CGAACAGGTCGACCCCGAGTTTGCCGACCGAGCCTCACAAAAAGGAGACGGGGCGGTAATCGGCGGCGAGAATTACGGGC
AGGGGTCGTCGCGCGAGCACGCCGCCCTGGCGCCGCGCTACTTAGGCATCAGGGTGAAGATCGCCAAAGGCTTCGCCAGG
ATCCATCGCTCCAACCTGATCAACTTCGGGATACTGCCGCTCACCTTCAAGAACAAGGAAGACTACGACCGGATTAAGCA
GGGAGACCGCCTGCAGTTAACGAACCTGCGCGAATTGATCCAGGGGGGCGCGACCGAGATACCGGTCAAATTCAACGGAG
GCGAGATCCTTACCCAGCTCACTGTTTCGGAGCGGGAGCGGGAGGAACTTCTGGCCGGGGGGCTTTTGAACAAGGTGAAG
GAGGAGACCAATGGCGGACAATCTTAA

Upstream 100 bases:

>100_bases
ACATAACAACGCAGTGGTGCTGAAAGAGCTTATCGACAAGGCGCGCAAGTGATCTAGCGTCAGGGTTAGCAACCGGCAGG
TGAGGGGGCGAGGAGGCAGC

Downstream 100 bases:

>100_bases
CGACCTGCTTCCCGAGGAGGATCTGCTGAAATTCTACGAGCAGATGGTGCTTTGCCGCGAGTTCGAGGAGAGCTGTGCCG
AGCAGTACTCCAAGGGGCAT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MGDNVAMKILKEHLVEGKLVPGQEIALRIDQTLLQDATGTMAMLEFMAMGLPRVKVELAAQYVDHNLLQTDNKNADDHAF
LTSAAQKFGVYLSKPGNGVSHQVSLERFGVPGTTLLGADSHTPTAAGLGQLAIGAGGLDVALAMAGHPFHLTCPRIWGVK
LTGQLQPWVSGKDVILEMLRRHSVKGGVGKIIEYYGPGVATLSATDRATIGNMGAELGATSTVFPSDQHTLAFLKAQGRG
EVWRELLPDPDATYDDHDEIDLSQVVPLIACPSSPDNVVPVSEIEGLKVDQVIVGSSVNSSFRDLMTVCRILDGRKVAPG
VAFHINPGSRQVLENVVAQGGFIMLLLAGGQVHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKEDRVYLCSPETA
AAAGIFGVVTDPRKLSDLLFWPAVQNPEQYLVDDAGIIVPPEGGSAVEIVTGPNIVPFPEFPALPDQLDVEVIIKVGDNI
STDSIMPAGNKVLPFRSNIPAISKFVFEQVDPEFADRASQKGDGAVIGGENYGQGSSREHAALAPRYLGIRVKIAKGFAR
IHRSNLINFGILPLTFKNKEDYDRIKQGDRLQLTNLRELIQGGATEIPVKFNGGEILTQLTVSEREREELLAGGLLNKVK
EETNGGQS

Sequences:

>Translated_648_residues
MGDNVAMKILKEHLVEGKLVPGQEIALRIDQTLLQDATGTMAMLEFMAMGLPRVKVELAAQYVDHNLLQTDNKNADDHAF
LTSAAQKFGVYLSKPGNGVSHQVSLERFGVPGTTLLGADSHTPTAAGLGQLAIGAGGLDVALAMAGHPFHLTCPRIWGVK
LTGQLQPWVSGKDVILEMLRRHSVKGGVGKIIEYYGPGVATLSATDRATIGNMGAELGATSTVFPSDQHTLAFLKAQGRG
EVWRELLPDPDATYDDHDEIDLSQVVPLIACPSSPDNVVPVSEIEGLKVDQVIVGSSVNSSFRDLMTVCRILDGRKVAPG
VAFHINPGSRQVLENVVAQGGFIMLLLAGGQVHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKEDRVYLCSPETA
AAAGIFGVVTDPRKLSDLLFWPAVQNPEQYLVDDAGIIVPPEGGSAVEIVTGPNIVPFPEFPALPDQLDVEVIIKVGDNI
STDSIMPAGNKVLPFRSNIPAISKFVFEQVDPEFADRASQKGDGAVIGGENYGQGSSREHAALAPRYLGIRVKIAKGFAR
IHRSNLINFGILPLTFKNKEDYDRIKQGDRLQLTNLRELIQGGATEIPVKFNGGEILTQLTVSEREREELLAGGLLNKVK
EETNGGQS
>Mature_647_residues
GDNVAMKILKEHLVEGKLVPGQEIALRIDQTLLQDATGTMAMLEFMAMGLPRVKVELAAQYVDHNLLQTDNKNADDHAFL
TSAAQKFGVYLSKPGNGVSHQVSLERFGVPGTTLLGADSHTPTAAGLGQLAIGAGGLDVALAMAGHPFHLTCPRIWGVKL
TGQLQPWVSGKDVILEMLRRHSVKGGVGKIIEYYGPGVATLSATDRATIGNMGAELGATSTVFPSDQHTLAFLKAQGRGE
VWRELLPDPDATYDDHDEIDLSQVVPLIACPSSPDNVVPVSEIEGLKVDQVIVGSSVNSSFRDLMTVCRILDGRKVAPGV
AFHINPGSRQVLENVVAQGGFIMLLLAGGQVHQPGCLGCIGMGQAPGTNQVSLRTFPRNFPGRSGTKEDRVYLCSPETAA
AAGIFGVVTDPRKLSDLLFWPAVQNPEQYLVDDAGIIVPPEGGSAVEIVTGPNIVPFPEFPALPDQLDVEVIIKVGDNIS
TDSIMPAGNKVLPFRSNIPAISKFVFEQVDPEFADRASQKGDGAVIGGENYGQGSSREHAALAPRYLGIRVKIAKGFARI
HRSNLINFGILPLTFKNKEDYDRIKQGDRLQLTNLRELIQGGATEIPVKFNGGEILTQLTVSEREREELLAGGLLNKVKE
ETNGGQS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=702, Percent_Identity=36.039886039886, Blast_Score=329, Evalue=4e-90,
Organism=Homo sapiens, GI8659555, Length=816, Percent_Identity=26.593137254902, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI41352693, Length=741, Percent_Identity=24.8313090418354, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081781, Length=733, Percent_Identity=26.8758526603001, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1787531, Length=748, Percent_Identity=26.3368983957219, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=28.3261802575107, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI2367097, Length=413, Percent_Identity=23.9709443099274, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=717, Percent_Identity=35.2859135285914, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI32564738, Length=684, Percent_Identity=35.672514619883, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25149342, Length=569, Percent_Identity=34.4463971880492, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17568399, Length=758, Percent_Identity=24.934036939314, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323335, Length=721, Percent_Identity=35.3675450762829, Blast_Score=353, Evalue=6e-98,
Organism=Saccharomyces cerevisiae, GI6322261, Length=731, Percent_Identity=33.7893296853625, Blast_Score=335, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6320440, Length=618, Percent_Identity=28.3171521035599, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321429, Length=653, Percent_Identity=27.1056661562021, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI28571643, Length=692, Percent_Identity=33.5260115606936, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI281365315, Length=719, Percent_Identity=33.7969401947149, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI17864292, Length=719, Percent_Identity=33.7969401947149, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI161076999, Length=686, Percent_Identity=34.1107871720117, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI17137564, Length=765, Percent_Identity=26.4052287581699, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645686, Length=753, Percent_Identity=26.4276228419655, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69340; Mature: 69209

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNVAMKILKEHLVEGKLVPGQEIALRIDQTLLQDATGTMAMLEFMAMGLPRVKVELAA
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QYVDHNLLQTDNKNADDHAFLTSAAQKFGVYLSKPGNGVSHQVSLERFGVPGTTLLGADS
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHCCCCCCEEECCCC
HTPTAAGLGQLAIGAGGLDVALAMAGHPFHLTCPRIWGVKLTGQLQPWVSGKDVILEMLR
CCCCHHCCCCCEECCCCHHEEEEECCCCEEEECCCEECEEEECCCCCCCCCHHHHHHHHH
RHSVKGGVGKIIEYYGPGVATLSATDRATIGNMGAELGATSTVFPSDQHTLAFLKAQGRG
HCCCCCCHHHHHHHHCCCEEEEECCCCCHHCCCCHHCCCCCEECCCCCCEEEEEEECCCH
EVWRELLPDPDATYDDHDEIDLSQVVPLIACPSSPDNVVPVSEIEGLKVDQVIVGSSVNS
HHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCCCCCEEEHHHCCCCEECEEEECCCCCH
SFRDLMTVCRILDGRKVAPGVAFHINPGSRQVLENVVAQGGFIMLLLAGGQVHQPGCLGC
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEE
IGMGQAPGTNQVSLRTFPRNFPGRSGTKEDRVYLCSPETAAAAGIFGVVTDPRKLSDLLF
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCEEEEECCCHHHHHHEE
WPAVQNPEQYLVDDAGIIVPPEGGSAVEIVTGPNIVPFPEFPALPDQLDVEVIIKVGDNI
CCCCCCCHHHEECCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCC
STDSIMPAGNKVLPFRSNIPAISKFVFEQVDPEFADRASQKGDGAVIGGENYGQGSSREH
CCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCCCCCCCCCC
AALAPRYLGIRVKIAKGFARIHRSNLINFGILPLTFKNKEDYDRIKQGDRLQLTNLRELI
HHCCCEEEEEEEEEHHHHHHHHHHCCCEECEEEEEECCCHHHHHHCCCCCEEHHHHHHHH
QGGATEIPVKFNGGEILTQLTVSEREREELLAGGLLNKVKEETNGGQS
HCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GDNVAMKILKEHLVEGKLVPGQEIALRIDQTLLQDATGTMAMLEFMAMGLPRVKVELAA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHH
QYVDHNLLQTDNKNADDHAFLTSAAQKFGVYLSKPGNGVSHQVSLERFGVPGTTLLGADS
HHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEHHHCCCCCCEEECCCC
HTPTAAGLGQLAIGAGGLDVALAMAGHPFHLTCPRIWGVKLTGQLQPWVSGKDVILEMLR
CCCCHHCCCCCEECCCCHHEEEEECCCCEEEECCCEECEEEECCCCCCCCCHHHHHHHHH
RHSVKGGVGKIIEYYGPGVATLSATDRATIGNMGAELGATSTVFPSDQHTLAFLKAQGRG
HCCCCCCHHHHHHHHCCCEEEEECCCCCHHCCCCHHCCCCCEECCCCCCEEEEEEECCCH
EVWRELLPDPDATYDDHDEIDLSQVVPLIACPSSPDNVVPVSEIEGLKVDQVIVGSSVNS
HHHHHHCCCCCCCCCCCCCCCHHHCCEEEECCCCCCCEEEHHHCCCCEECEEEECCCCCH
SFRDLMTVCRILDGRKVAPGVAFHINPGSRQVLENVVAQGGFIMLLLAGGQVHQPGCLGC
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEE
IGMGQAPGTNQVSLRTFPRNFPGRSGTKEDRVYLCSPETAAAAGIFGVVTDPRKLSDLLF
EECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHCEEEEECCCHHHHHHEE
WPAVQNPEQYLVDDAGIIVPPEGGSAVEIVTGPNIVPFPEFPALPDQLDVEVIIKVGDNI
CCCCCCCHHHEECCCCEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCC
STDSIMPAGNKVLPFRSNIPAISKFVFEQVDPEFADRASQKGDGAVIGGENYGQGSSREH
CCCCCCCCCCEEECCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCEEECCCCCCCCCCCCC
AALAPRYLGIRVKIAKGFARIHRSNLINFGILPLTFKNKEDYDRIKQGDRLQLTNLRELI
HHCCCEEEEEEEEEHHHHHHHHHHCCCEECEEEEEECCCHHHHHHCCCCCEEHHHHHHHH
QGGATEIPVKFNGGEILTQLTVSEREREELLAGGLLNKVKEETNGGQS
HCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]