Click here to switch to the map view.

The map label for this gene is livK-2 [H]

Identifier: 197118571

GI number: 197118571

Start: 2549795

End: 2550934

Strand: Reverse

Name: livK-2 [H]

Synonym: Gbem_2190

Alternate gene names: 197118571

Gene position: 2550934-2549795 (Counterclockwise)

Preceding gene: 197118572

Following gene: 197118570

Centisome position: 55.27

GC content: 61.4

Gene sequence:

>1140_bases
ATGAGAAAGATTCTGGTGGTACTCAGCATCATGATGCTTCTGTGCAGCGCCACTGCCGCACTCGCCGCGGCGCCGATCAA
GATCGGCGGTCTCTTCGCCGTGACCGGTCCCGCAGCTTTCCTCGGCGAACCGGAGAAGAACACGCTGGAGCTGCTGGTTA
AAGAGGCCAACGCCAAGGGCGGCATCGGCGGCTCGAAGATCGAACTGGTCATCTACGACACCGGCGGCGACGTCACCAAG
GCGGTCCAGCTAGCCAACAAGCTGATCAAGAACGACAAGGTCTCCGCCATTGTCGGCCCCAGCACCACCGGCGAAACGAT
GGCCGTCATCCCCATCGCCGAGAAGGAGCAGATCCCGCTCGTCTCCTGCGCCGCCGGCATCAAGATCACCGACCCGGTGA
AGAAATGGGTCTTCAAGACCCCGGCCAACGACCACGTCGCCGCCGAAAAGATCCTGCTCCAGGCGTCCAAACTCAAGCAA
AAGAACATCGCTCTTCTCACCGTTTCCGACTCCTTCGGCTCTTCCGGTCGCGAGCAGCTGAAGCAGATGGCGGCAAAGCA
CGGCTTCAAGGTCGTGGCGGACGAGGTCTACGGGCCTAAAGATACCGACATGACCGCGCAGCTCACCAAGATCAAGGCAG
CCAAGCCCGATGCCATCATCTGCTGGGGGACCAATCCCGGCCCGGCCATCATCACCCGCAACGTGCAGCAGTTGGGCATC
AAGGCCCAGCTCTATCAAAGCCACGGCGTCGCCTCCAAGAAGTACATCGAGCTCGCCAGCGCCCAGGCCGCTCAGGGGGT
CATGCTCCCTGCCGGCAAGCTCGCCGTATTCGACCTCTTGAAGAAGACCGACCCGCAGGCGAAGCTCCTCAAGGAGTACA
ACGACTCCTACAAGAAGGCCTACGGCATCGAGGCGTCCACCTTCGGCGGCTACGCCTACGACGGCTTCCAGTTGATCGCC
GCTGCTGTGAAAAATGGGGCCGTCACCCCGGCGCAGATCCGCGACGGCATAGAGAAGGGTGGGAGCATGGTCGGGGTGTC
CGGTGTCTTCAAGATGACCCCCAAAGATCATAACGGACTCGACCTCTCCGCCTTCGAGATGGTCCGCATCGATAAAGGCG
ACTGGGCGATCGTACGCTGA

Upstream 100 bases:

>100_bases
GCACTGTCCAGCTCTCCCCCTCCCTTTACGGGAGGGGGGACTTTTACGGCGTAGTGCTTTTGGTAGCGGTGATCGACTGA
AACATTTTTCAGGGGGGAAG

Downstream 100 bases:

>100_bases
TTTAATCGCGGCAATGCTGCCGCTCCTTCAAGGGGGGAATATCCGTGAGAAAACTGTTTACCACTGCTCTTGCACTATTT
ACCTTGCTCTGCCTTTCCAC

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRKILVVLSIMMLLCSATAALAAAPIKIGGLFAVTGPAAFLGEPEKNTLELLVKEANAKGGIGGSKIELVIYDTGGDVTK
AVQLANKLIKNDKVSAIVGPSTTGETMAVIPIAEKEQIPLVSCAAGIKITDPVKKWVFKTPANDHVAAEKILLQASKLKQ
KNIALLTVSDSFGSSGREQLKQMAAKHGFKVVADEVYGPKDTDMTAQLTKIKAAKPDAIICWGTNPGPAIITRNVQQLGI
KAQLYQSHGVASKKYIELASAQAAQGVMLPAGKLAVFDLLKKTDPQAKLLKEYNDSYKKAYGIEASTFGGYAYDGFQLIA
AAVKNGAVTPAQIRDGIEKGGSMVGVSGVFKMTPKDHNGLDLSAFEMVRIDKGDWAIVR

Sequences:

>Translated_379_residues
MRKILVVLSIMMLLCSATAALAAAPIKIGGLFAVTGPAAFLGEPEKNTLELLVKEANAKGGIGGSKIELVIYDTGGDVTK
AVQLANKLIKNDKVSAIVGPSTTGETMAVIPIAEKEQIPLVSCAAGIKITDPVKKWVFKTPANDHVAAEKILLQASKLKQ
KNIALLTVSDSFGSSGREQLKQMAAKHGFKVVADEVYGPKDTDMTAQLTKIKAAKPDAIICWGTNPGPAIITRNVQQLGI
KAQLYQSHGVASKKYIELASAQAAQGVMLPAGKLAVFDLLKKTDPQAKLLKEYNDSYKKAYGIEASTFGGYAYDGFQLIA
AAVKNGAVTPAQIRDGIEKGGSMVGVSGVFKMTPKDHNGLDLSAFEMVRIDKGDWAIVR
>Mature_379_residues
MRKILVVLSIMMLLCSATAALAAAPIKIGGLFAVTGPAAFLGEPEKNTLELLVKEANAKGGIGGSKIELVIYDTGGDVTK
AVQLANKLIKNDKVSAIVGPSTTGETMAVIPIAEKEQIPLVSCAAGIKITDPVKKWVFKTPANDHVAAEKILLQASKLKQ
KNIALLTVSDSFGSSGREQLKQMAAKHGFKVVADEVYGPKDTDMTAQLTKIKAAKPDAIICWGTNPGPAIITRNVQQLGI
KAQLYQSHGVASKKYIELASAQAAQGVMLPAGKLAVFDLLKKTDPQAKLLKEYNDSYKKAYGIEASTFGGYAYDGFQLIA
AAVKNGAVTPAQIRDGIEKGGSMVGVSGVFKMTPKDHNGLDLSAFEMVRIDKGDWAIVR

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=268, Percent_Identity=27.2388059701493, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789867, Length=273, Percent_Identity=26.007326007326, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40109; Mature: 40109

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILVVLSIMMLLCSATAALAAAPIKIGGLFAVTGPAAFLGEPEKNTLELLVKEANAKG
CHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEEECCCHHHCCCCCCCHHHHHEECCCCCC
GIGGSKIELVIYDTGGDVTKAVQLANKLIKNDKVSAIVGPSTTGETMAVIPIAEKEQIPL
CCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCE
VSCAAGIKITDPVKKWVFKTPANDHVAAEKILLQASKLKQKNIALLTVSDSFGSSGREQL
EEECCCCEECCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
KQMAAKHGFKVVADEVYGPKDTDMTAQLTKIKAAKPDAIICWGTNPGPAIITRNVQQLGI
HHHHHHCCCEEEEHHCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEEECCHHHHCC
KAQLYQSHGVASKKYIELASAQAAQGVMLPAGKLAVFDLLKKTDPQAKLLKEYNDSYKKA
EEEEHHHCCCCHHHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YGIEASTFGGYAYDGFQLIAAAVKNGAVTPAQIRDGIEKGGSMVGVSGVFKMTPKDHNGL
CCCCCCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECEEEEECCCCCCCC
DLSAFEMVRIDKGDWAIVR
CCEEEEEEEECCCCEEEEC
>Mature Secondary Structure
MRKILVVLSIMMLLCSATAALAAAPIKIGGLFAVTGPAAFLGEPEKNTLELLVKEANAKG
CHHHHHHHHHHHHHHHHHHHHHHCCEEECEEEEECCCHHHCCCCCCCHHHHHEECCCCCC
GIGGSKIELVIYDTGGDVTKAVQLANKLIKNDKVSAIVGPSTTGETMAVIPIAEKEQIPL
CCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCE
VSCAAGIKITDPVKKWVFKTPANDHVAAEKILLQASKLKQKNIALLTVSDSFGSSGREQL
EEECCCCEECCHHHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHH
KQMAAKHGFKVVADEVYGPKDTDMTAQLTKIKAAKPDAIICWGTNPGPAIITRNVQQLGI
HHHHHHCCCEEEEHHCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCEEEECCHHHHCC
KAQLYQSHGVASKKYIELASAQAAQGVMLPAGKLAVFDLLKKTDPQAKLLKEYNDSYKKA
EEEEHHHCCCCHHHHHHHHHHHHHCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
YGIEASTFGGYAYDGFQLIAAAVKNGAVTPAQIRDGIEKGGSMVGVSGVFKMTPKDHNGL
CCCCCCCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECEEEEECCCCCCCC
DLSAFEMVRIDKGDWAIVR
CCEEEEEEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]