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The map label for this gene is livK-1 [H]

Identifier: 197118570

GI number: 197118570

Start: 2548611

End: 2549750

Strand: Reverse

Name: livK-1 [H]

Synonym: Gbem_2189

Alternate gene names: 197118570

Gene position: 2549750-2548611 (Counterclockwise)

Preceding gene: 197118571

Following gene: 197118569

Centisome position: 55.25

GC content: 61.49

Gene sequence:

>1140_bases
GTGAGAAAACTGTTTACCACTGCTCTTGCACTATTTACCTTGCTCTGCCTTTCCACCGTGGCCTTTGCCGCCGCTCCCAT
TAAGATCGGCGCGCTTTTCGCCGTGACCGGCCCCGCCTCTTTCCTGGGGGAGCCTGAGCGCAACACCGCTCAGATGGTGG
TCGACGAGATCAACAAGGCAGGCGGCGTAAAAGGTCGCAAGATCGAACTGATCACCTACGACACCGGTGGCGACGCCACC
AAGGCCGTGCAGCTCGCCAACAAGCTGGTCAAGAACGACAATGTCGTGGCCATCATCGGTCCCAGCACCACCGGCGACAG
CATGGCTATCATCCCGGTGGTCGAGAAGGCCGGGATCCCGCTCATCTCCTGCGCTGCCGGGAGCAAGATCACCGAGCCGG
TGAAAAAGTGGGTTTTCAAGACCGCCCAGAACGACGGGCTCGCCGTCGCCAGGATCTATGAGCAGCTGAAAAAAGAGAAA
AAGACCAAGGTGGCGATACTCACCGTCTCCGACGGCTTCGGCTCCTCCGGGCGCGAGCAGTTGAAGGCCCAGGCGAAGGT
CCATGGCATCCAGATCATTTCCGACGACACCTACGGCCCGAAGGACACCGACATGACCGCGCAGCTCGCCAAGATCCGCG
GCTCGCAGGCGCAGGCGGTGATCTGCTGGGGCACCAACCCCGGTCCCGCCGTGGTGGCAAGAAACGCCAAGCAGCTCGGC
CTCAAAATCCCGCTCTACATGAGCCACGGCGTTTCCTCCAAGAAGTTCATCGAGCTGGCCGGGGACTCGGCAGAGGGTAT
CAGGCTCCCCTCCGGGAAGGTGCTGGTCGCCGACCTGCTGCCGAAAACCGACAGGCAGAAAGGGTCGCTCCTTGCCTTCA
TCAAGGACTACCAGAACCACTACAAGGCCGAGGGCGACCACTTCGGCGGCCACGCCTGGGACGCGGTTATGCTCCTTAAA
GGTGCCATCGAGAGGGGAGGGGACACCCCCGCCGGTATCCGCAACGCTCTGGAGGCAACCCGCAACTTCGCCGGCATCGG
CGGCGTTTTCAACTTCTCGCCCAGGGACCACGCAGGCCTGACGAAAGACGCCTTCACCCTGGTTGAAGTACGCAAAAAAG
ACTGGGTGCTGGTCAAGTAA

Upstream 100 bases:

>100_bases
TCTCCGCCTTCGAGATGGTCCGCATCGATAAAGGCGACTGGGCGATCGTACGCTGATTTAATCGCGGCAATGCTGCCGCT
CCTTCAAGGGGGGAATATCC

Downstream 100 bases:

>100_bases
AGAGGATTCATGGAGCTTTTAAATCAGGTATCGCAATTCGTCATATCGGGGCTCGCCACCGGCGCCATCTACGCCCTGAT
TGGCCTCAGCTTCGCGATCA

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRKLFTTALALFTLLCLSTVAFAAAPIKIGALFAVTGPASFLGEPERNTAQMVVDEINKAGGVKGRKIELITYDTGGDAT
KAVQLANKLVKNDNVVAIIGPSTTGDSMAIIPVVEKAGIPLISCAAGSKITEPVKKWVFKTAQNDGLAVARIYEQLKKEK
KTKVAILTVSDGFGSSGREQLKAQAKVHGIQIISDDTYGPKDTDMTAQLAKIRGSQAQAVICWGTNPGPAVVARNAKQLG
LKIPLYMSHGVSSKKFIELAGDSAEGIRLPSGKVLVADLLPKTDRQKGSLLAFIKDYQNHYKAEGDHFGGHAWDAVMLLK
GAIERGGDTPAGIRNALEATRNFAGIGGVFNFSPRDHAGLTKDAFTLVEVRKKDWVLVK

Sequences:

>Translated_379_residues
MRKLFTTALALFTLLCLSTVAFAAAPIKIGALFAVTGPASFLGEPERNTAQMVVDEINKAGGVKGRKIELITYDTGGDAT
KAVQLANKLVKNDNVVAIIGPSTTGDSMAIIPVVEKAGIPLISCAAGSKITEPVKKWVFKTAQNDGLAVARIYEQLKKEK
KTKVAILTVSDGFGSSGREQLKAQAKVHGIQIISDDTYGPKDTDMTAQLAKIRGSQAQAVICWGTNPGPAVVARNAKQLG
LKIPLYMSHGVSSKKFIELAGDSAEGIRLPSGKVLVADLLPKTDRQKGSLLAFIKDYQNHYKAEGDHFGGHAWDAVMLLK
GAIERGGDTPAGIRNALEATRNFAGIGGVFNFSPRDHAGLTKDAFTLVEVRKKDWVLVK
>Mature_379_residues
MRKLFTTALALFTLLCLSTVAFAAAPIKIGALFAVTGPASFLGEPERNTAQMVVDEINKAGGVKGRKIELITYDTGGDAT
KAVQLANKLVKNDNVVAIIGPSTTGDSMAIIPVVEKAGIPLISCAAGSKITEPVKKWVFKTAQNDGLAVARIYEQLKKEK
KTKVAILTVSDGFGSSGREQLKAQAKVHGIQIISDDTYGPKDTDMTAQLAKIRGSQAQAVICWGTNPGPAVVARNAKQLG
LKIPLYMSHGVSSKKFIELAGDSAEGIRLPSGKVLVADLLPKTDRQKGSLLAFIKDYQNHYKAEGDHFGGHAWDAVMLLK
GAIERGGDTPAGIRNALEATRNFAGIGGVFNFSPRDHAGLTKDAFTLVEVRKKDWVLVK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=26.3610315186246, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994941, Length=296, Percent_Identity=25.6756756756757, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40351; Mature: 40351

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTTALALFTLLCLSTVAFAAAPIKIGALFAVTGPASFLGEPERNTAQMVVDEINKA
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHH
GGVKGRKIELITYDTGGDATKAVQLANKLVKNDNVVAIIGPSTTGDSMAIIPVVEKAGIP
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCC
LISCAAGSKITEPVKKWVFKTAQNDGLAVARIYEQLKKEKKTKVAILTVSDGFGSSGREQ
EEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
LKAQAKVHGIQIISDDTYGPKDTDMTAQLAKIRGSQAQAVICWGTNPGPAVVARNAKQLG
HHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCHHHCC
LKIPLYMSHGVSSKKFIELAGDSAEGIRLPSGKVLVADLLPKTDRQKGSLLAFIKDYQNH
EEEEEEECCCCCCCEEEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
YKAEGDHFGGHAWDAVMLLKGAIERGGDTPAGIRNALEATRNFAGIGGVFNFSPRDHAGL
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
TKDAFTLVEVRKKDWVLVK
CHHHHHHEEECCCCEEEEC
>Mature Secondary Structure
MRKLFTTALALFTLLCLSTVAFAAAPIKIGALFAVTGPASFLGEPERNTAQMVVDEINKA
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCHHHCCCCCCCHHHHHHHHHHHH
GGVKGRKIELITYDTGGDATKAVQLANKLVKNDNVVAIIGPSTTGDSMAIIPVVEKAGIP
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCCCC
LISCAAGSKITEPVKKWVFKTAQNDGLAVARIYEQLKKEKKTKVAILTVSDGFGSSGREQ
EEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
LKAQAKVHGIQIISDDTYGPKDTDMTAQLAKIRGSQAQAVICWGTNPGPAVVARNAKQLG
HHHHHHEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEECCHHHCC
LKIPLYMSHGVSSKKFIELAGDSAEGIRLPSGKVLVADLLPKTDRQKGSLLAFIKDYQNH
EEEEEEECCCCCCCEEEEEECCCCCCEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
YKAEGDHFGGHAWDAVMLLKGAIERGGDTPAGIRNALEATRNFAGIGGVFNFSPRDHAGL
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCC
TKDAFTLVEVRKKDWVLVK
CHHHHHHEEECCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]