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The map label for this gene is nreB [H]

Identifier: 197118302

GI number: 197118302

Start: 2217672

End: 2219141

Strand: Direct

Name: nreB [H]

Synonym: Gbem_1919

Alternate gene names: 197118302

Gene position: 2217672-2219141 (Clockwise)

Preceding gene: 308535272

Following gene: 197118303

Centisome position: 48.05

GC content: 55.37

Gene sequence:

>1470_bases
ATGATTTTTAGCAGACTGAGACATGCAAACTACGCTCTGGCTCTCCTGGCAACCGGAGCCGCCTTTTCGCTGCGCCAGTT
GGTTCAATACCTGACGGGAGCAGATCTTCCTCTCTATATCGCCTTCTTCCCCATCGTGTTGATAGTGCTCCTTGCGATTG
GCCGGACACCGGCAATCCTTGTCGCAGCTGCGGCGGCGGCATATTCCGCTTACTGGCATCTCTCCCCGACGGGCAGTAAT
AACGTCGCAAATTCCTCTGATGGCGTCGGTCTTGCGCTGTTTGTCTTGTCTGGTGCCTGCTTGTGTCTACTTTCAGAGAT
CGTGCGGCAAAAGGTCGATGCCTACCAATCTGCAAACGAACAACTCGTGCGGGAGATAAGCCTGCGCAGGCAGACGGAGG
AATATCTGGCAAGCCGCGAGAGAGACCTGTCGCAGACCCAGATGGCAACCCATCTTGGCAGCTGGAAGTGGGATATTGTG
AACGATAAGGTGACTTGGTCCAAGGAGTTGTACCGGATTTTCGGCGTGGATCCGCAGACCTTCATTCCGACAAATACCAG
GGCCAACGAATTGATTCATCCGGATGATCGGGACATGCACAACAATTTTGTTGCCATGGCGCTGACCGGCGTGTCGGTTG
GGCCGTTCGAGTGCCGCCTCTGCCCCCCGGGGGGAGAGGAGCGGATCGTTCTGGCGAGCGGCTTTGACGTCGAATTTGAT
TCATTCGGCAACCCCGTCTCGCTCCTTGGGACCGTCATTGACCTCACCGAGCAAAAACTGGCTGAACGCAAAATTTCCGA
GTATCAGCAAAAACTTCAATCAATGGTGATCGAACTGTCTATGGCCGAGGAGCGGGAGCGGTGCCGCATCGCAGGGGAGT
TGCACGATCAGGTCGGTCAGAATCTCATCCTGTGCAAAATGAAGCTCGACGCGCTGATGGAACTGCTACAGTGCGGCGAG
CCTTTCGATGTGGCTGCGGGGCTTGAGACGATTATAGACCAAGCGATCCAGGACATCCGGTCGCTCACCTTCCAGATGAG
GCCGTCGATACTTGTCACCGGAGGCCTGGGCCCCGCCTTGCACTGGCTGAGTGAGGAGTTGCACGCAACGTCTGGGCTGA
AAGTGGAACTTAAGGCCGACTACAGCCACAAACCGCTCAGACATGATGTTAATCAGACTGTTTTTCAGGCTGCGCGCGAA
CTGATGTTGAATGTCGCCAAACATGCTGGAACCAATGCCTGCCGAGTTACCTTGAATTTTGACGACGATTTCCTCACGCT
TTGCGTCGAAGACGATGGCATCGGTTTACAGGCCAGGTCCTCACAAGGCACCAACACCATGAAGGGCGGATTCGGTCTCT
TCAACGTGCAACAAAGAATTGAGCATCTGGGCGGCAGGTTTTCCATCGAGGACAAGGTGGCAGGAGGCGTTCTGGCAACC
ATCATGGTCCCGACGGGAGCGTGTGGCTGA

Upstream 100 bases:

>100_bases
GACGGGGGGCGACGGGGCCAGGCATTGATAAATGGTAACTCGGGAAGAAGGTCCCATCAATTGTTCCTTGTAGAGGAGGA
GATGGTGTGCCAGGAATACT

Downstream 100 bases:

>100_bases
CCGTGAAAACAGACTCCGCGGGCTGGCAATAGCTGATAGCAGCCGGGAATAAGCCGAGCCGCTCCGGGGGCTGCACCTCA
GCAAGAAACGTCAGGTTATC

Product: PAS domain-containing sensor histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MIFSRLRHANYALALLATGAAFSLRQLVQYLTGADLPLYIAFFPIVLIVLLAIGRTPAILVAAAAAAYSAYWHLSPTGSN
NVANSSDGVGLALFVLSGACLCLLSEIVRQKVDAYQSANEQLVREISLRRQTEEYLASRERDLSQTQMATHLGSWKWDIV
NDKVTWSKELYRIFGVDPQTFIPTNTRANELIHPDDRDMHNNFVAMALTGVSVGPFECRLCPPGGEERIVLASGFDVEFD
SFGNPVSLLGTVIDLTEQKLAERKISEYQQKLQSMVIELSMAEERERCRIAGELHDQVGQNLILCKMKLDALMELLQCGE
PFDVAAGLETIIDQAIQDIRSLTFQMRPSILVTGGLGPALHWLSEELHATSGLKVELKADYSHKPLRHDVNQTVFQAARE
LMLNVAKHAGTNACRVTLNFDDDFLTLCVEDDGIGLQARSSQGTNTMKGGFGLFNVQQRIEHLGGRFSIEDKVAGGVLAT
IMVPTGACG

Sequences:

>Translated_489_residues
MIFSRLRHANYALALLATGAAFSLRQLVQYLTGADLPLYIAFFPIVLIVLLAIGRTPAILVAAAAAAYSAYWHLSPTGSN
NVANSSDGVGLALFVLSGACLCLLSEIVRQKVDAYQSANEQLVREISLRRQTEEYLASRERDLSQTQMATHLGSWKWDIV
NDKVTWSKELYRIFGVDPQTFIPTNTRANELIHPDDRDMHNNFVAMALTGVSVGPFECRLCPPGGEERIVLASGFDVEFD
SFGNPVSLLGTVIDLTEQKLAERKISEYQQKLQSMVIELSMAEERERCRIAGELHDQVGQNLILCKMKLDALMELLQCGE
PFDVAAGLETIIDQAIQDIRSLTFQMRPSILVTGGLGPALHWLSEELHATSGLKVELKADYSHKPLRHDVNQTVFQAARE
LMLNVAKHAGTNACRVTLNFDDDFLTLCVEDDGIGLQARSSQGTNTMKGGFGLFNVQQRIEHLGGRFSIEDKVAGGVLAT
IMVPTGACG
>Mature_489_residues
MIFSRLRHANYALALLATGAAFSLRQLVQYLTGADLPLYIAFFPIVLIVLLAIGRTPAILVAAAAAAYSAYWHLSPTGSN
NVANSSDGVGLALFVLSGACLCLLSEIVRQKVDAYQSANEQLVREISLRRQTEEYLASRERDLSQTQMATHLGSWKWDIV
NDKVTWSKELYRIFGVDPQTFIPTNTRANELIHPDDRDMHNNFVAMALTGVSVGPFECRLCPPGGEERIVLASGFDVEFD
SFGNPVSLLGTVIDLTEQKLAERKISEYQQKLQSMVIELSMAEERERCRIAGELHDQVGQNLILCKMKLDALMELLQCGE
PFDVAAGLETIIDQAIQDIRSLTFQMRPSILVTGGLGPALHWLSEELHATSGLKVELKADYSHKPLRHDVNQTVFQAARE
LMLNVAKHAGTNACRVTLNFDDDFLTLCVEDDGIGLQARSSQGTNTMKGGFGLFNVQQRIEHLGGRFSIEDKVAGGVLAT
IMVPTGACG

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=309, Percent_Identity=24.9190938511327, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53727; Mature: 53727

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSRLRHANYALALLATGAAFSLRQLVQYLTGADLPLYIAFFPIVLIVLLAIGRTPAIL
CCHHHHHHCCCEEEEHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAAAAAAYSAYWHLSPTGSNNVANSSDGVGLALFVLSGACLCLLSEIVRQKVDAYQSANE
HHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVREISLRRQTEEYLASRERDLSQTQMATHLGSWKWDIVNDKVTWSKELYRIFGVDPQT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCC
FIPTNTRANELIHPDDRDMHNNFVAMALTGVSVGPFECRLCPPGGEERIVLASGFDVEFD
CCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCEECC
SFGNPVSLLGTVIDLTEQKLAERKISEYQQKLQSMVIELSMAEERERCRIAGELHDQVGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLILCKMKLDALMELLQCGEPFDVAAGLETIIDQAIQDIRSLTFQMRPSILVTGGLGPAL
CEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
HWLSEELHATSGLKVELKADYSHKPLRHDVNQTVFQAARELMLNVAKHAGTNACRVTLNF
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
DDDFLTLCVEDDGIGLQARSSQGTNTMKGGFGLFNVQQRIEHLGGRFSIEDKVAGGVLAT
CCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCEEEE
IMVPTGACG
EEECCCCCC
>Mature Secondary Structure
MIFSRLRHANYALALLATGAAFSLRQLVQYLTGADLPLYIAFFPIVLIVLLAIGRTPAIL
CCHHHHHHCCCEEEEHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAAAAAAYSAYWHLSPTGSNNVANSSDGVGLALFVLSGACLCLLSEIVRQKVDAYQSANE
HHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVREISLRRQTEEYLASRERDLSQTQMATHLGSWKWDIVNDKVTWSKELYRIFGVDPQT
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCC
FIPTNTRANELIHPDDRDMHNNFVAMALTGVSVGPFECRLCPPGGEERIVLASGFDVEFD
CCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCCCCEEEECCCCCCCEEEEEECCCEECC
SFGNPVSLLGTVIDLTEQKLAERKISEYQQKLQSMVIELSMAEERERCRIAGELHDQVGQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLILCKMKLDALMELLQCGEPFDVAAGLETIIDQAIQDIRSLTFQMRPSILVTGGLGPAL
CEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHH
HWLSEELHATSGLKVELKADYSHKPLRHDVNQTVFQAARELMLNVAKHAGTNACRVTLNF
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
DDDFLTLCVEDDGIGLQARSSQGTNTMKGGFGLFNVQQRIEHLGGRFSIEDKVAGGVLAT
CCCEEEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCEEEE
IMVPTGACG
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]