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The map label for this gene is carA [H]

Identifier: 197118281

GI number: 197118281

Start: 2197742

End: 2198866

Strand: Direct

Name: carA [H]

Synonym: Gbem_1898

Alternate gene names: 197118281

Gene position: 2197742-2198866 (Clockwise)

Preceding gene: 197118280

Following gene: 197118282

Centisome position: 47.62

GC content: 61.42

Gene sequence:

>1125_bases
ATGAAAGCAGTACTTGCTCTGGCGGACGGCCGGATTTTCAAGGGTAAGGCCTTCGGTGCGACGGGCGAAACAAGCGGCGA
GGTGGTGTTCAATACCGCCATGTCCGGCTATCAGGAAGTCCTCACCGACCCTTCCTACAAGGGGCAGATGGTCACCATGA
CCTACACCCAGATCGGCAACACCGGCATCAACCCGGAAGATGTGGAAAGCGGGCAGCTCTACCTGTCCGGCTTCATCGTC
AGGGAGTACCTGGACTGCTATTCCAACTACCGCGCCACCATGAGCCTGGACGCTTACCTCAAGGAGAACGGCATCGTCGG
CATCCAGGGGATCGACACCCGCGCCCTGACCCGCCACCTGCGCGACAAGGGGGCCCAAAACGGCATCATCTCCACCATCG
ACTTCGATCCGGAGAGCCTGGCCAGGAAGGCGCGCGCCATCCCCTCCATGAGCGGTCTGGACCTTGCCACCGGCGTCACC
TGCAGCGCCCCGTACCACTGGACCGAAGGTCTGTGGGACCTGAAGAGCGGCTACCCGCAGGTCGACCGCAAGGATTTGAA
GTACAAGGTCGTGGCCTACGACTTCGGCATCAAGCTGAACATCCTGCGCTGCCTGGTTTCCGCCGGTTGCGACGTCACCG
TGGTTCCGGCGACCTTCCCTGCGGAGTCCGCGCTCGCCATGAATCCGGACGGCATCTTCCTTTCCAACGGCCCGGGCGAC
CCTGAGCCGATGAAGGAAGTCATCGAGAACATCAAGAAATTCGTCGGCAAGAAGCCGATCTTCGGCATCTGCCTTGGACA
CCAGCTCATGGGCCTTGCCCTGGGCGGCCGCACCATCAAGCTCAAGTTCGGCAACCACGGCTCCAACCTTCCGGTCATGG
ATATGGCCACGAGAAAGGTCGAGATCACCGCCCAGAACCACGGCTTCTCCGTCGATATCCTGTCGCTTAGCAACGTCGCC
GGCTTGGCGCACGAGAACCTGAACGACCAGACCGTCGAAGGTATGGCGCACAAGACCCTCCCCATCTTCTCGGTGCAGCA
CCACCCCGAGGCGTCCCCCGGGCCGCACGACTCGCACTACCTGTTCGACAGGTTCGTCGAGATGATGGAGAAGCATAAGG
CGTAG

Upstream 100 bases:

>100_bases
TCGCGCCACATGCTCCCCCTCCCTTGACGGGAGGGGGAAGTTAAAACGAAATTTGCGCTTTTTTGCGCGGCAGAAATATA
ACGGACTTGGGAGAAGAAAC

Downstream 100 bases:

>100_bases
GAAAGGACAGAGGGGAGGAGCCGGCGGCTTGGTCATCGATGGCGACTGCCCCCACCCCCTAACCCCCTCCCGCAAGGGGA
GGGGGGATAGAGAGACCCCC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKAVLALADGRIFKGKAFGATGETSGEVVFNTAMSGYQEVLTDPSYKGQMVTMTYTQIGNTGINPEDVESGQLYLSGFIV
REYLDCYSNYRATMSLDAYLKENGIVGIQGIDTRALTRHLRDKGAQNGIISTIDFDPESLARKARAIPSMSGLDLATGVT
CSAPYHWTEGLWDLKSGYPQVDRKDLKYKVVAYDFGIKLNILRCLVSAGCDVTVVPATFPAESALAMNPDGIFLSNGPGD
PEPMKEVIENIKKFVGKKPIFGICLGHQLMGLALGGRTIKLKFGNHGSNLPVMDMATRKVEITAQNHGFSVDILSLSNVA
GLAHENLNDQTVEGMAHKTLPIFSVQHHPEASPGPHDSHYLFDRFVEMMEKHKA

Sequences:

>Translated_374_residues
MKAVLALADGRIFKGKAFGATGETSGEVVFNTAMSGYQEVLTDPSYKGQMVTMTYTQIGNTGINPEDVESGQLYLSGFIV
REYLDCYSNYRATMSLDAYLKENGIVGIQGIDTRALTRHLRDKGAQNGIISTIDFDPESLARKARAIPSMSGLDLATGVT
CSAPYHWTEGLWDLKSGYPQVDRKDLKYKVVAYDFGIKLNILRCLVSAGCDVTVVPATFPAESALAMNPDGIFLSNGPGD
PEPMKEVIENIKKFVGKKPIFGICLGHQLMGLALGGRTIKLKFGNHGSNLPVMDMATRKVEITAQNHGFSVDILSLSNVA
GLAHENLNDQTVEGMAHKTLPIFSVQHHPEASPGPHDSHYLFDRFVEMMEKHKA
>Mature_374_residues
MKAVLALADGRIFKGKAFGATGETSGEVVFNTAMSGYQEVLTDPSYKGQMVTMTYTQIGNTGINPEDVESGQLYLSGFIV
REYLDCYSNYRATMSLDAYLKENGIVGIQGIDTRALTRHLRDKGAQNGIISTIDFDPESLARKARAIPSMSGLDLATGVT
CSAPYHWTEGLWDLKSGYPQVDRKDLKYKVVAYDFGIKLNILRCLVSAGCDVTVVPATFPAESALAMNPDGIFLSNGPGD
PEPMKEVIENIKKFVGKKPIFGICLGHQLMGLALGGRTIKLKFGNHGSNLPVMDMATRKVEITAQNHGFSVDILSLSNVA
GLAHENLNDQTVEGMAHKTLPIFSVQHHPEASPGPHDSHYLFDRFVEMMEKHKA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=384, Percent_Identity=38.28125, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=37.8516624040921, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=37.8516624040921, Blast_Score=235, Evalue=4e-62,
Organism=Escherichia coli, GI1786215, Length=374, Percent_Identity=53.7433155080214, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=37.4358974358974, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6324878, Length=388, Percent_Identity=39.1752577319588, Blast_Score=266, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322331, Length=401, Percent_Identity=35.9102244389027, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI45555749, Length=387, Percent_Identity=36.9509043927649, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24642586, Length=387, Percent_Identity=37.2093023255814, Blast_Score=241, Evalue=5e-64,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40766; Mature: 40766

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLALADGRIFKGKAFGATGETSGEVVFNTAMSGYQEVLTDPSYKGQMVTMTYTQIGN
CCEEEEECCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINPEDVESGQLYLSGFIVREYLDCYSNYRATMSLDAYLKENGIVGIQGIDTRALTRHL
CCCCCCCCCCCEEEEHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCHHHHHHHH
RDKGAQNGIISTIDFDPESLARKARAIPSMSGLDLATGVTCSAPYHWTEGLWDLKSGYPQ
HHCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCHHCCCEECCCCCHHCCCHHHHCCCCC
VDRKDLKYKVVAYDFGIKLNILRCLVSAGCDVTVVPATFPAESALAMNPDGIFLSNGPGD
CCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCC
PEPMKEVIENIKKFVGKKPIFGICLGHQLMGLALGGRTIKLKFGNHGSNLPVMDMATRKV
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEECCEEEEEEECCCCCCCCEEEECCEEE
EITAQNHGFSVDILSLSNVAGLAHENLNDQTVEGMAHKTLPIFSVQHHPEASPGPHDSHY
EEEECCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCHH
LFDRFVEMMEKHKA
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKAVLALADGRIFKGKAFGATGETSGEVVFNTAMSGYQEVLTDPSYKGQMVTMTYTQIGN
CCEEEEECCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEEEEECCC
TGINPEDVESGQLYLSGFIVREYLDCYSNYRATMSLDAYLKENGIVGIQGIDTRALTRHL
CCCCCCCCCCCEEEEHHHHHHHHHHHHHCCEEEEEEEEEECCCCEEEEECCCHHHHHHHH
RDKGAQNGIISTIDFDPESLARKARAIPSMSGLDLATGVTCSAPYHWTEGLWDLKSGYPQ
HHCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCHHCCCEECCCCCHHCCCHHHHCCCCC
VDRKDLKYKVVAYDFGIKLNILRCLVSAGCDVTVVPATFPAESALAMNPDGIFLSNGPGD
CCCCCCEEEEEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCC
PEPMKEVIENIKKFVGKKPIFGICLGHQLMGLALGGRTIKLKFGNHGSNLPVMDMATRKV
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHEEECCEEEEEEECCCCCCCCEEEECCEEE
EITAQNHGFSVDILSLSNVAGLAHENLNDQTVEGMAHKTLPIFSVQHHPEASPGPHDSHY
EEEECCCCCEEEEEECCCHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCHH
LFDRFVEMMEKHKA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA