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The map label for this gene is pstS-4 [H]

Identifier: 197118235

GI number: 197118235

Start: 2145918

End: 2146946

Strand: Direct

Name: pstS-4 [H]

Synonym: Gbem_1852

Alternate gene names: 197118235

Gene position: 2145918-2146946 (Clockwise)

Preceding gene: 308535268

Following gene: 197118236

Centisome position: 46.5

GC content: 60.06

Gene sequence:

>1029_bases
ATGATCAAGAGCATTAAAAAAGCATTCCTGACCTTGGCCCTGATGGCAGCATTTGGTACCGCAGCGCAGGCATCCGCCGA
GACCCTCATCAACGGCGCCGGCGCCACCTTCCCGTACCCGCTTTACTCCAAGTGGTTCACCGAGTACGCCAAGGTGGATA
AAGCGGTGAAGTTCAACTATCAGTCCATCGGCAGCGGCGGCGGCATCAAGCAGATCAGCGCGCAGACCGTCGACTTCGGC
GCGAGCGACAAGTTCCTCTCCGACCAGGAGCTCAATGCAGCTCCGGGCAAGCTGATCCACATCCCGACCGTCATGGGCGC
CGTGGTAGTGACCTACAACGTCCCGGGCGTGGTTAGCGGGCTGAAACTCAACTCCGAGGACGTCGCCAACATCTACCTCG
GTAAGATCACCATGTGGAACGACCCGAGGATCGCCGACGACAACAAGGGGGTGAACCTCCCGGCCAAGCCGATCATCGTC
GTGCACCGCTCCGATGGGTCCGGCACCACCAGCATCTTCACCGACTACCTGAACGGGATGAACCGCGAATGGGCGCAGAA
GGTCGGCAAGGGCGCTTCGGTGAAGTGGCCGGTTGGCCTTGGCGGCAAGGGTAACGAGGGTGTCGCGGGGCAGGTGAAGA
CCACCCCGTATTCCATCGGCTACGTCGAACTCGCCTACGCCTTTGAGAACAAACTCCCCTACGCTTCGCTCAAGAACAAG
GCCGGCGTCTTCGTCGAACCCTCCATCAAGTCCACCAGTGCGGCAGCCGCCGCAGCCGTCAAGGACATGCCGGCTGATTA
TCGCATCTCCCTGGTGAATCAGCCGGGCAAGGACGCCTATCCCGTGGTCGGATTTACCTGGCTCTTGGTGTACGAGCACC
AGAAGGATGCAGCCAAAGGGAAGCAGATGGTCGAGTTCTTGAACTGGGCCATGACCAAGGGGCAGAAGATGGCCGCCCCG
ATGCTCTACGCGCCGCTGCCGGAGAGCGTCGTGAAAATGGTGCAGAAAACCATCAAGACCATCAAATAG

Upstream 100 bases:

>100_bases
CGCCCGCGGCAACTATTCACAGACTCTTGCCATGACTGTAACGAGATCGCGACATTGCTCGATTATAGTCCTCGGCAAAT
CCAGAAAGGAGACATTCAAA

Downstream 100 bases:

>100_bases
GTCCCCACGAAGAACAGTGCCCCGCGGGGCGGCAAATGCCGCCCCGCGGGATTTGCCTTCCCCGCCGTCCCCTCGTATCA
CCCGGCTGATCCTTCTGGTT

Product: phosphate ABC transporter periplasmic phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIKSIKKAFLTLALMAAFGTAAQASAETLINGAGATFPYPLYSKWFTEYAKVDKAVKFNYQSIGSGGGIKQISAQTVDFG
ASDKFLSDQELNAAPGKLIHIPTVMGAVVVTYNVPGVVSGLKLNSEDVANIYLGKITMWNDPRIADDNKGVNLPAKPIIV
VHRSDGSGTTSIFTDYLNGMNREWAQKVGKGASVKWPVGLGGKGNEGVAGQVKTTPYSIGYVELAYAFENKLPYASLKNK
AGVFVEPSIKSTSAAAAAAVKDMPADYRISLVNQPGKDAYPVVGFTWLLVYEHQKDAAKGKQMVEFLNWAMTKGQKMAAP
MLYAPLPESVVKMVQKTIKTIK

Sequences:

>Translated_342_residues
MIKSIKKAFLTLALMAAFGTAAQASAETLINGAGATFPYPLYSKWFTEYAKVDKAVKFNYQSIGSGGGIKQISAQTVDFG
ASDKFLSDQELNAAPGKLIHIPTVMGAVVVTYNVPGVVSGLKLNSEDVANIYLGKITMWNDPRIADDNKGVNLPAKPIIV
VHRSDGSGTTSIFTDYLNGMNREWAQKVGKGASVKWPVGLGGKGNEGVAGQVKTTPYSIGYVELAYAFENKLPYASLKNK
AGVFVEPSIKSTSAAAAAAVKDMPADYRISLVNQPGKDAYPVVGFTWLLVYEHQKDAAKGKQMVEFLNWAMTKGQKMAAP
MLYAPLPESVVKMVQKTIKTIK
>Mature_342_residues
MIKSIKKAFLTLALMAAFGTAAQASAETLINGAGATFPYPLYSKWFTEYAKVDKAVKFNYQSIGSGGGIKQISAQTVDFG
ASDKFLSDQELNAAPGKLIHIPTVMGAVVVTYNVPGVVSGLKLNSEDVANIYLGKITMWNDPRIADDNKGVNLPAKPIIV
VHRSDGSGTTSIFTDYLNGMNREWAQKVGKGASVKWPVGLGGKGNEGVAGQVKTTPYSIGYVELAYAFENKLPYASLKNK
AGVFVEPSIKSTSAAAAAAVKDMPADYRISLVNQPGKDAYPVVGFTWLLVYEHQKDAAKGKQMVEFLNWAMTKGQKMAAP
MLYAPLPESVVKMVQKTIKTIK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=327, Percent_Identity=47.0948012232416, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36717; Mature: 36717

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSIKKAFLTLALMAAFGTAAQASAETLINGAGATFPYPLYSKWFTEYAKVDKAVKFNY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECH
QSIGSGGGIKQISAQTVDFGASDKFLSDQELNAAPGKLIHIPTVMGAVVVTYNVPGVVSG
HHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEECCCCCEEC
LKLNSEDVANIYLGKITMWNDPRIADDNKGVNLPAKPIIVVHRSDGSGTTSIFTDYLNGM
CEECCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCC
NREWAQKVGKGASVKWPVGLGGKGNEGVAGQVKTTPYSIGYVELAYAFENKLPYASLKNK
CHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCEECCCEEEEEEHHCCCCHHHHCCC
AGVFVEPSIKSTSAAAAAAVKDMPADYRISLVNQPGKDAYPVVGFTWLLVYEHQKDAAKG
CCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCHHHHHH
KQMVEFLNWAMTKGQKMAAPMLYAPLPESVVKMVQKTIKTIK
HHHHHHHHHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKSIKKAFLTLALMAAFGTAAQASAETLINGAGATFPYPLYSKWFTEYAKVDKAVKFNY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECH
QSIGSGGGIKQISAQTVDFGASDKFLSDQELNAAPGKLIHIPTVMGAVVVTYNVPGVVSG
HHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHEEEEEEECCCCCEEC
LKLNSEDVANIYLGKITMWNDPRIADDNKGVNLPAKPIIVVHRSDGSGTTSIFTDYLNGM
CEECCCCCCEEEEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCC
NREWAQKVGKGASVKWPVGLGGKGNEGVAGQVKTTPYSIGYVELAYAFENKLPYASLKNK
CHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCEECCCEEEEEEHHCCCCHHHHCCC
AGVFVEPSIKSTSAAAAAAVKDMPADYRISLVNQPGKDAYPVVGFTWLLVYEHQKDAAKG
CCEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHEEEEEEEEECCHHHHHH
KQMVEFLNWAMTKGQKMAAPMLYAPLPESVVKMVQKTIKTIK
HHHHHHHHHHHCCCCHHCCCEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]