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The map label for this gene is lexA-1

Identifier: 197118143

GI number: 197118143

Start: 2026113

End: 2026718

Strand: Reverse

Name: lexA-1

Synonym: Gbem_1758

Alternate gene names: 197118143

Gene position: 2026718-2026113 (Counterclockwise)

Preceding gene: 197118145

Following gene: 197118142

Centisome position: 43.91

GC content: 60.23

Gene sequence:

>606_bases
ATGGAACAGCTAACGGCACGACAGACGGAGGTGCTTCAGATCATCACCCGGCATCTGGAGACGTGCGGCTACCCCCCTAC
CCTGCGTGAGATTGCTGCGCAGCTTGGCATTTCGGGGACACTGGGAGTAATGAAGCATCTGGAAGCCCTGGAGAAGAAAG
GATACCTGCGCCGGCAGGAGGGGAGTACCAGGGGGATTACGCTGTGCAACCAAAGCCAGGCGGCCAGCCTCCCCATAGTG
GGAGTGGTGCGCGCGGGCCTGCTGCACCCGGCCATACAAGACATCGAGGGGCACTTCGCCATCGACCGCTCTCAACTCGC
TTCGGGTGGCGCTTTCTTCTTGCGGGTCAAAGGGGACTCGATGGTCCACGCCCATATTGTGGAGGGGGACCTAGCGCTGG
TGCGTCCCCAACCCCACGCCTCGAATCGGGATATCGTGGTGGCCATGGTGGATGGGGAGGCCACGCTGAAAAGGTTCTAC
CGCGAAGCGGACCGCATCAGACTACAACCCGAGAACCCGAACTACGAGCCGATCATAATCGAGAAGGGGGAGCAGGAGGT
GTCTATAGTGGGGAAAGTTGTTGGCATCTACCGGCAAATGGAGTAG

Upstream 100 bases:

>100_bases
AAAAACTAACAAAACTTGCTAAAAAAGGATGATTTCCATCTTGACAGGGCATGGTTGTCATTTGGTAAGGTTGCCATAAG
AAAACCAACGGAGGATCGTC

Downstream 100 bases:

>100_bases
TTTGATGCCGCCCGTTGCCGAAAATGTCAGAGTCGCCGCCATCTTCACCCCTGGAACACAAATCAAGCCGGTATGGTTCG
AATGGCGTTGCCGCAAGCAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEQLTARQTEVLQIITRHLETCGYPPTLREIAAQLGISGTLGVMKHLEALEKKGYLRRQEGSTRGITLCNQSQAASLPIV
GVVRAGLLHPAIQDIEGHFAIDRSQLASGGAFFLRVKGDSMVHAHIVEGDLALVRPQPHASNRDIVVAMVDGEATLKRFY
READRIRLQPENPNYEPIIIEKGEQEVSIVGKVVGIYRQME

Sequences:

>Translated_201_residues
MEQLTARQTEVLQIITRHLETCGYPPTLREIAAQLGISGTLGVMKHLEALEKKGYLRRQEGSTRGITLCNQSQAASLPIV
GVVRAGLLHPAIQDIEGHFAIDRSQLASGGAFFLRVKGDSMVHAHIVEGDLALVRPQPHASNRDIVVAMVDGEATLKRFY
READRIRLQPENPNYEPIIIEKGEQEVSIVGKVVGIYRQME
>Mature_201_residues
MEQLTARQTEVLQIITRHLETCGYPPTLREIAAQLGISGTLGVMKHLEALEKKGYLRRQEGSTRGITLCNQSQAASLPIV
GVVRAGLLHPAIQDIEGHFAIDRSQLASGGAFFLRVKGDSMVHAHIVEGDLALVRPQPHASNRDIVVAMVDGEATLKRFY
READRIRLQPENPNYEPIIIEKGEQEVSIVGKVVGIYRQME

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=199, Percent_Identity=39.6984924623116, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787431, Length=122, Percent_Identity=34.4262295081967, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_GEOBB (B5EA68)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138570.1
- ProteinModelPortal:   B5EA68
- SMR:   B5EA68
- MEROPS:   S24.001
- GeneID:   6781747
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1758
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   CLSK827607
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006594
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- SMART:   SM00667
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22227; Mature: 22227

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: ACT_SITE 120-120 ACT_SITE 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLTARQTEVLQIITRHLETCGYPPTLREIAAQLGISGTLGVMKHLEALEKKGYLRRQE
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCC
GSTRGITLCNQSQAASLPIVGVVRAGLLHPAIQDIEGHFAIDRSQLASGGAFFLRVKGDS
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCEEEEEECCCC
MVHAHIVEGDLALVRPQPHASNRDIVVAMVDGEATLKRFYREADRIRLQPENPNYEPIII
EEEEEEEECCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHEEECCCCCCCCEEEE
EKGEQEVSIVGKVVGIYRQME
ECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEQLTARQTEVLQIITRHLETCGYPPTLREIAAQLGISGTLGVMKHLEALEKKGYLRRQE
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCC
GSTRGITLCNQSQAASLPIVGVVRAGLLHPAIQDIEGHFAIDRSQLASGGAFFLRVKGDS
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCEEEEEECCCC
MVHAHIVEGDLALVRPQPHASNRDIVVAMVDGEATLKRFYREADRIRLQPENPNYEPIII
EEEEEEEECCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHEEECCCCCCCCEEEE
EKGEQEVSIVGKVVGIYRQME
ECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA