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The map label for this gene is algU [H]

Identifier: 197118082

GI number: 197118082

Start: 1961471

End: 1962031

Strand: Direct

Name: algU [H]

Synonym: Gbem_1696

Alternate gene names: 197118082

Gene position: 1961471-1962031 (Clockwise)

Preceding gene: 197118081

Following gene: 197118083

Centisome position: 42.5

GC content: 59.89

Gene sequence:

>561_bases
ATGACCTTTGAAGAGATCTATCAGGAGTTCCAGCCCAAGATAACCCGTTATCTCTGCAGTTTCATGTCCGAGGAAGAAGC
GCTCGAAGTGAGCCAGGCGGTTTTCCTGAAGGTGAGCCAATCCCTCGAAAGCTTCAGGGCGGAATCCTCCCTCTCCACCT
GGATCTACCGGATCGCCACCAACGCCGCGCACGACCACGCCTCTTTGTCCATGACCAAGCAAAGGCGGGCTGAGCAACTG
CTCGACGAGGACCAGTCCCTGGACGATTTCGTGCGCGAGGACGACCCGGAGACCGACTGGGAGTACATCCGGAGCGAGAT
GGGGTGCTGTATCCGCGGGATGGTGGACGGCCTGCCGGAAAAGCTCCGCGAGGTCCTGGTCTTGAGCGAGTTCGAGGGAC
TTTCCAATGCCGAGATTGCAGATGTTTTGAAGGCGAGTCTCGACACCGTGAAGATCCGGCTCCACCGGGCGCGGAAGGCC
TTGCGCGAGGCGATGGGAGCCGGGTGCGACTTCTACCGCGACGAGCGCAACCAACTGATGTGCGACCGAAAGGCGCAGTA
A

Upstream 100 bases:

>100_bases
CAAAACAAAGGCCTCTGGGTTCAACCCAAAGTCTTTTGCCTTTCTCTGCGACCTCCGTGATCCTCTGTGACCTCTGTGGT
AAAGCTTTTAAGGAGTTTGC

Downstream 100 bases:

>100_bases
AAAAAAGGGGACAGGCTACTTTAAAGGGGACAGGCTACTTTTTGATTTGAAAAAGTAGCCTGTCCCCTTTTTTGTATCCT
TTTTCGTGGAAGCCCGTCTG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTFEEIYQEFQPKITRYLCSFMSEEEALEVSQAVFLKVSQSLESFRAESSLSTWIYRIATNAAHDHASLSMTKQRRAEQL
LDEDQSLDDFVREDDPETDWEYIRSEMGCCIRGMVDGLPEKLREVLVLSEFEGLSNAEIADVLKASLDTVKIRLHRARKA
LREAMGAGCDFYRDERNQLMCDRKAQ

Sequences:

>Translated_186_residues
MTFEEIYQEFQPKITRYLCSFMSEEEALEVSQAVFLKVSQSLESFRAESSLSTWIYRIATNAAHDHASLSMTKQRRAEQL
LDEDQSLDDFVREDDPETDWEYIRSEMGCCIRGMVDGLPEKLREVLVLSEFEGLSNAEIADVLKASLDTVKIRLHRARKA
LREAMGAGCDFYRDERNQLMCDRKAQ
>Mature_185_residues
TFEEIYQEFQPKITRYLCSFMSEEEALEVSQAVFLKVSQSLESFRAESSLSTWIYRIATNAAHDHASLSMTKQRRAEQLL
DEDQSLDDFVREDDPETDWEYIRSEMGCCIRGMVDGLPEKLREVLVLSEFEGLSNAEIADVLKASLDTVKIRLHRARKAL
REAMGAGCDFYRDERNQLMCDRKAQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=166, Percent_Identity=28.9156626506024, Blast_Score=73, Evalue=9e-15,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21520; Mature: 21389

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEEIYQEFQPKITRYLCSFMSEEEALEVSQAVFLKVSQSLESFRAESSLSTWIYRIAT
CCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAAHDHASLSMTKQRRAEQLLDEDQSLDDFVREDDPETDWEYIRSEMGCCIRGMVDGLPE
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KLREVLVLSEFEGLSNAEIADVLKASLDTVKIRLHRARKALREAMGAGCDFYRDERNQLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
CDRKAQ
CCCCCC
>Mature Secondary Structure 
TFEEIYQEFQPKITRYLCSFMSEEEALEVSQAVFLKVSQSLESFRAESSLSTWIYRIAT
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAAHDHASLSMTKQRRAEQLLDEDQSLDDFVREDDPETDWEYIRSEMGCCIRGMVDGLPE
HCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
KLREVLVLSEFEGLSNAEIADVLKASLDTVKIRLHRARKALREAMGAGCDFYRDERNQLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
CDRKAQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]