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The map label for this gene is lacA [C]

Identifier: 197117998

GI number: 197117998

Start: 1869583

End: 1870185

Strand: Direct

Name: lacA [C]

Synonym: Gbem_1611

Alternate gene names: 197117998

Gene position: 1869583-1870185 (Clockwise)

Preceding gene: 197117997

Following gene: 197117999

Centisome position: 40.51

GC content: 60.36

Gene sequence:

>603_bases
GTGAGAGCCCTCTACCGTCTGCTGCTTGTTTTACTCACTCGCATGCGCCACGCTTGGTATCGGCTCTGCCTGAGGTTCCA
GACCACGGCGCTTAGGGGCGGACTAAACGTCGGCAAGAACGTTCTGCTGCAGGTCCCACTGCGCTGTGACGGATACGGCA
AGGTATCCATTGGCGACGACACGGCCTTCGGCTCACGCCCCGCGCCTAAGGCAGGCAATGGCGAGGTGTTGATTCAGGCG
CGCGCCGCCGAAGCCGTTATCCAGATCGGACGTAACGTCCTTTTCTCCAACAACATCTCGCTCATCTCCACGGTCGGGAT
AACCATCGGCGATGACTGCCTGGTAGGGGACAATCTGCTTGTGATGGATACCGACTTCCACGGCATTCACCCTGCCGAAC
GCAGGACCAGTCCCGGCACGTCGAGGCCGGTCACGGTCGGCAAGAACGTCTGGTTCGGAAGCAGGGTAATCGTGCAGAAA
GGTGTCACCATCGGCGACAACTCGGTCATCGCCTCGCAGTCGGTAGTGACGCGCGACATTCCCCCAAACTCTGTGGCCGG
GGGCAATCCCGCCAAAGTACTTAGGACCCTCGATGGCTGCTGA

Upstream 100 bases:

>100_bases
TCACTGAGGCCATCCATCGCGAAGTGCTAAGCCTGCCCATGGGGCCACATCTCCTGCAGTCGGAAGTCCAAAGCGTCATT
GATACAGCGGGACGGTGCCT

Downstream 100 bases:

>100_bases
GACTGGGACTACAGCTGAAAAAAGGGGCTCCATCGGGCACATACTGAAGGCGTCCGCGGTCATGGGGAGTTCGGCTGTGG
TCCAGATCGCCACAGGAATC

Product: acyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MRALYRLLLVLLTRMRHAWYRLCLRFQTTALRGGLNVGKNVLLQVPLRCDGYGKVSIGDDTAFGSRPAPKAGNGEVLIQA
RAAEAVIQIGRNVLFSNNISLISTVGITIGDDCLVGDNLLVMDTDFHGIHPAERRTSPGTSRPVTVGKNVWFGSRVIVQK
GVTIGDNSVIASQSVVTRDIPPNSVAGGNPAKVLRTLDGC

Sequences:

>Translated_200_residues
MRALYRLLLVLLTRMRHAWYRLCLRFQTTALRGGLNVGKNVLLQVPLRCDGYGKVSIGDDTAFGSRPAPKAGNGEVLIQA
RAAEAVIQIGRNVLFSNNISLISTVGITIGDDCLVGDNLLVMDTDFHGIHPAERRTSPGTSRPVTVGKNVWFGSRVIVQK
GVTIGDNSVIASQSVVTRDIPPNSVAGGNPAKVLRTLDGC
>Mature_200_residues
MRALYRLLLVLLTRMRHAWYRLCLRFQTTALRGGLNVGKNVLLQVPLRCDGYGKVSIGDDTAFGSRPAPKAGNGEVLIQA
RAAEAVIQIGRNVLFSNNISLISTVGITIGDDCLVGDNLLVMDTDFHGIHPAERRTSPGTSRPVTVGKNVWFGSRVIVQK
GVTIGDNSVIASQSVVTRDIPPNSVAGGNPAKVLRTLDGC

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=112, Percent_Identity=44.6428571428571, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1786664, Length=111, Percent_Identity=40.5405405405405, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788345, Length=200, Percent_Identity=27.5, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788367, Length=124, Percent_Identity=31.4516129032258, Blast_Score=60, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322243, Length=111, Percent_Identity=42.3423423423423, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21447; Mature: 21447

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALYRLLLVLLTRMRHAWYRLCLRFQTTALRGGLNVGKNVLLQVPLRCDGYGKVSIGDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEECCCCEEEECCC
TAFGSRPAPKAGNGEVLIQARAAEAVIQIGRNVLFSNNISLISTVGITIGDDCLVGDNLL
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECCCEEEEEECEEECCCEEECCCEE
VMDTDFHGIHPAERRTSPGTSRPVTVGKNVWFGSRVIVQKGVTIGDNSVIASQSVVTRDI
EEECCCCCCCCHHHCCCCCCCCCEEECCCEECCCEEEEECCCEECCCCEEECCCEEECCC
PPNSVAGGNPAKVLRTLDGC
CCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRALYRLLLVLLTRMRHAWYRLCLRFQTTALRGGLNVGKNVLLQVPLRCDGYGKVSIGDD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECEEECCCCEEEECCC
TAFGSRPAPKAGNGEVLIQARAAEAVIQIGRNVLFSNNISLISTVGITIGDDCLVGDNLL
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHCCCEEEECCCEEEEEECEEECCCEEECCCEE
VMDTDFHGIHPAERRTSPGTSRPVTVGKNVWFGSRVIVQKGVTIGDNSVIASQSVVTRDI
EEECCCCCCCCHHHCCCCCCCCCEEECCCEECCCEEEEECCCEECCCCEEECCCEEECCC
PPNSVAGGNPAKVLRTLDGC
CCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA