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The map label for this gene is gcvH-2 [H]

Identifier: 197117950

GI number: 197117950

Start: 1812147

End: 1812536

Strand: Direct

Name: gcvH-2 [H]

Synonym: Gbem_1563

Alternate gene names: 197117950

Gene position: 1812147-1812536 (Clockwise)

Preceding gene: 197117949

Following gene: 197117951

Centisome position: 39.27

GC content: 58.72

Gene sequence:

>390_bases
ATGGACTTTCCCGAAGAGCTTAAATACAGCAAGGAACACGTGTGGGTGCGCATCGAAGGCGAGCGCGCCGTAATCGGCAT
TACCGATTATGCCCAGACCGAATTGGGCAACATTACTTCCGTCGAGCTTCCCGAACCTGGCGACGAGTTGGAGCAGGACG
ATTCCTTCGGGTCCGTCGAGGCGAGGAAGACCGTTGCCGAGCTGTACGCCCCGCTTTCCGGCACCGTGCTGGAGGTAAAC
TCAGAACTGGGGAACGCGCCGGAGTTCGTCAACGACGATCCCTACGACGCGGGGTGGCTGGTGGTCATCGAGGTGGCGGA
CTCCGAGGAATCCAATCTCCTCATGTCCGCCGAACACTACGAGGATTACGTCGCCGTAGCGGAAGATTAA

Upstream 100 bases:

>100_bases
GACCACCATTCCGTTCCACAAGAGGATCATGGCCAACAAAGACTTCATCGAAGGGAACATAGACACCGGCTTCATCGAAA
GGCTGGTACTGGAGTAAACT

Downstream 100 bases:

>100_bases
GAATCAGATGCACATATACAAGAAAAGGCGAGAGCATCTCTCGCCTTTTCCTTTTGCAGCCCCCGGAGTTCTTCAGTGAC
TATTGACATCGGACACATCA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MDFPEELKYSKEHVWVRIEGERAVIGITDYAQTELGNITSVELPEPGDELEQDDSFGSVEARKTVAELYAPLSGTVLEVN
SELGNAPEFVNDDPYDAGWLVVIEVADSEESNLLMSAEHYEDYVAVAED

Sequences:

>Translated_129_residues
MDFPEELKYSKEHVWVRIEGERAVIGITDYAQTELGNITSVELPEPGDELEQDDSFGSVEARKTVAELYAPLSGTVLEVN
SELGNAPEFVNDDPYDAGWLVVIEVADSEESNLLMSAEHYEDYVAVAED
>Mature_129_residues
MDFPEELKYSKEHVWVRIEGERAVIGITDYAQTELGNITSVELPEPGDELEQDDSFGSVEARKTVAELYAPLSGTVLEVN
SELGNAPEFVNDDPYDAGWLVVIEVADSEESNLLMSAEHYEDYVAVAED

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=39.344262295082, Blast_Score=101, Evalue=1e-22,
Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=38.5245901639344, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=41.3223140495868, Blast_Score=99, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=39.1304347826087, Blast_Score=105, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=37.3913043478261, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=41.8032786885246, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17865652, Length=128, Percent_Identity=37.5, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14314; Mature: 14314

Theoretical pI: Translated: 3.59; Mature: 3.59

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPEELKYSKEHVWVRIEGERAVIGITDYAQTELGNITSVELPEPGDELEQDDSFGSVE
CCCCHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCH
ARKTVAELYAPLSGTVLEVNSELGNAPEFVNDDPYDAGWLVVIEVADSEESNLLMSAEHY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCC
EDYVAVAED
CCEEEEECC
>Mature Secondary Structure
MDFPEELKYSKEHVWVRIEGERAVIGITDYAQTELGNITSVELPEPGDELEQDDSFGSVE
CCCCHHCCCCCCEEEEEECCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCH
ARKTVAELYAPLSGTVLEVNSELGNAPEFVNDDPYDAGWLVVIEVADSEESNLLMSAEHY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCC
EDYVAVAED
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA