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The map label for this gene is zraS [H]

Identifier: 197117920

GI number: 197117920

Start: 1777628

End: 1779094

Strand: Direct

Name: zraS [H]

Synonym: Gbem_1533

Alternate gene names: 197117920

Gene position: 1777628-1779094 (Clockwise)

Preceding gene: 197117919

Following gene: 197117921

Centisome position: 38.52

GC content: 62.51

Gene sequence:

>1467_bases
ATGAAACCTACCCGGTTCAGCCTCACCTTCTATACCCTCTCCGCCCTGAGCATCATGCTGGTGCTCACCTGGGTGCTTCT
TTCCGTAATCTCCTTCAACACCGCCGAAAAAGACCTGCTGAACCAAAAGGGGGAAGAAGGAAAGCTGTTGCTTTCCAGCA
TCGTCCTTCTGCTGCCGCGTCCTTTGGCCCCGCCCGAACCAGGTTCGGCCGCGGCCAAGTACCTGGAGCACCTTAGGAAG
GACCGTGCCTTCGTGGGGCTGCTGGTGATCGATTCCGCGGGGCGAGACCTCTACACCCTCTCCGATCCGGGCGGGGCCAA
TGCCGCTTTGCTCGGCGTATTGCGCCGTGGGCGCGGGACCTTCCAGGTGTCGCCGGACGGCCGGCTTGCGTGGTGCTACC
TTCCGGTCATGGAGCAGGGGAGGGCGATCGGGGCGGCTCGGCTCACCCTGTCGCTGCTTCCCGAGCGGGAAAAGCTCAGA
GGTTCCCGGAACCTTTTTCTTTCCTACTTCATCCTCGATTTCCTGCTGCTCCTGGTGGTCGGCTCATATCTTCTTTCCTC
AAGCGTAGTTGTGCCGATAAGAAGACTGTTGGCGGCGACCCGCAGAATCGCGGCGGGGGATTTGAGCGGCACCGTGGACA
TCCCCGGAAGCCAGGAAGTGGCGGAACTGGGCGACGCCTTCAATACGATGGTGGTGGCGCTCAGGGAGAAGAGGGAGGAG
GTCGAGGCAAATGTAGCCTCCTTGAACCGCGCCAACCGGGAGATCATCGAGGCGCGCAACGAGGCGATACGTTCCGAGAA
GATGGCGTCCGTTGGGCTTTTGGCGGCCGGGATGGCACACGAAATCGGGACTCCGCTTGCGGCCATCATGGGGTACAGCG
GGATTCTCTCCGAGGAGGTTGCCGGCGACCCGGAGAAGTCAGATTACCTGCGCAGGATCACCGAAGAGTCGAAGCGGATC
GACAGGATCGTCAGGGGGCTTTTGGACTATGCCCGGCCCAAGGGTAGCCAGCGTGAGGAAATAGAAGTGCTGCAGCTGTT
GGAGAAGGTGGTGGAGCTTCTGGAGGCACAGGGGGCGTTGAAGCTCTTGGAGGTCACGGTCGAGGCGGAGCCGTCCCTGC
CGCGGCTGTATGCCGACCCGCACCAGTTGGAGCAGCTGCTCATCAACCTCATCATGAATGCCAAGGATGCCATGCCCAAG
GGGGGCAAGCTGCAGCTCAAGGGGATGAGCGTGGGAGGGGGCGTCGCCATCGAGGTTGCCGACACCGGTGAAGGTATGCC
GCCCGAGCACCTGCCGCTGGTATTCGACCCCTTCTTCACCACCAAGGAGCCCGGTAAGGGAACCGGTCTGGGGCTAGCCA
TCGCGGCCAGGATAGCCGAGTCGTGCGGCGGGACACTCACCGTGCAAAGCGAGTTGGGAGAGGGGAGCCGGTTCAGGCTG
ACGCTGCCGGCGGGAGGCAGTGGCTAG

Upstream 100 bases:

>100_bases
CCATTCGGCCCCTTCCTGGCAAGCGGCGCCGTGCTCTACATCTTCTACGGCAACAGAATCATTCACTGGTACCTGAGCAT
GGGAAGCTAAAGCGTCCCGT

Downstream 100 bases:

>100_bases
GGGCTGGGGCCGCAAGGTCAGACAGGTCAGACAGGTCAGACAGGCTGGGCGTTTAGGAGAAAGTGTGACCGAGAAGAAGA
AAATACTGGTGGTGGACGAC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKPTRFSLTFYTLSALSIMLVLTWVLLSVISFNTAEKDLLNQKGEEGKLLLSSIVLLLPRPLAPPEPGSAAAKYLEHLRK
DRAFVGLLVIDSAGRDLYTLSDPGGANAALLGVLRRGRGTFQVSPDGRLAWCYLPVMEQGRAIGAARLTLSLLPEREKLR
GSRNLFLSYFILDFLLLLVVGSYLLSSSVVVPIRRLLAATRRIAAGDLSGTVDIPGSQEVAELGDAFNTMVVALREKREE
VEANVASLNRANREIIEARNEAIRSEKMASVGLLAAGMAHEIGTPLAAIMGYSGILSEEVAGDPEKSDYLRRITEESKRI
DRIVRGLLDYARPKGSQREEIEVLQLLEKVVELLEAQGALKLLEVTVEAEPSLPRLYADPHQLEQLLINLIMNAKDAMPK
GGKLQLKGMSVGGGVAIEVADTGEGMPPEHLPLVFDPFFTTKEPGKGTGLGLAIAARIAESCGGTLTVQSELGEGSRFRL
TLPAGGSG

Sequences:

>Translated_488_residues
MKPTRFSLTFYTLSALSIMLVLTWVLLSVISFNTAEKDLLNQKGEEGKLLLSSIVLLLPRPLAPPEPGSAAAKYLEHLRK
DRAFVGLLVIDSAGRDLYTLSDPGGANAALLGVLRRGRGTFQVSPDGRLAWCYLPVMEQGRAIGAARLTLSLLPEREKLR
GSRNLFLSYFILDFLLLLVVGSYLLSSSVVVPIRRLLAATRRIAAGDLSGTVDIPGSQEVAELGDAFNTMVVALREKREE
VEANVASLNRANREIIEARNEAIRSEKMASVGLLAAGMAHEIGTPLAAIMGYSGILSEEVAGDPEKSDYLRRITEESKRI
DRIVRGLLDYARPKGSQREEIEVLQLLEKVVELLEAQGALKLLEVTVEAEPSLPRLYADPHQLEQLLINLIMNAKDAMPK
GGKLQLKGMSVGGGVAIEVADTGEGMPPEHLPLVFDPFFTTKEPGKGTGLGLAIAARIAESCGGTLTVQSELGEGSRFRL
TLPAGGSG
>Mature_488_residues
MKPTRFSLTFYTLSALSIMLVLTWVLLSVISFNTAEKDLLNQKGEEGKLLLSSIVLLLPRPLAPPEPGSAAAKYLEHLRK
DRAFVGLLVIDSAGRDLYTLSDPGGANAALLGVLRRGRGTFQVSPDGRLAWCYLPVMEQGRAIGAARLTLSLLPEREKLR
GSRNLFLSYFILDFLLLLVVGSYLLSSSVVVPIRRLLAATRRIAAGDLSGTVDIPGSQEVAELGDAFNTMVVALREKREE
VEANVASLNRANREIIEARNEAIRSEKMASVGLLAAGMAHEIGTPLAAIMGYSGILSEEVAGDPEKSDYLRRITEESKRI
DRIVRGLLDYARPKGSQREEIEVLQLLEKVVELLEAQGALKLLEVTVEAEPSLPRLYADPHQLEQLLINLIMNAKDAMPK
GGKLQLKGMSVGGGVAIEVADTGEGMPPEHLPLVFDPFFTTKEPGKGTGLGLAIAARIAESCGGTLTVQSELGEGSRFRL
TLPAGGSG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=35.1111111111111, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788549, Length=233, Percent_Identity=33.9055793991416, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081816, Length=344, Percent_Identity=29.3604651162791, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789149, Length=349, Percent_Identity=25.7879656160458, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=31.6455696202532, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=30.316742081448, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=27.6497695852535, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI48994928, Length=222, Percent_Identity=31.0810810810811, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=513, Percent_Identity=24.1715399610136, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=370, Percent_Identity=26.7567567567568, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=248, Percent_Identity=31.0483870967742, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790346, Length=303, Percent_Identity=29.042904290429, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788393, Length=290, Percent_Identity=23.448275862069, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788279, Length=218, Percent_Identity=27.5229357798165, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787894, Length=301, Percent_Identity=26.2458471760797, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52613; Mature: 52613

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTRFSLTFYTLSALSIMLVLTWVLLSVISFNTAEKDLLNQKGEEGKLLLSSIVLLLPR
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCC
PLAPPEPGSAAAKYLEHLRKDRAFVGLLVIDSAGRDLYTLSDPGGANAALLGVLRRGRGT
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCE
FQVSPDGRLAWCYLPVMEQGRAIGAARLTLSLLPEREKLRGSRNLFLSYFILDFLLLLVV
EEECCCCCEEEEEECHHHCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
GSYLLSSSVVVPIRRLLAATRRIAAGDLSGTVDIPGSQEVAELGDAFNTMVVALREKREE
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VEANVASLNRANREIIEARNEAIRSEKMASVGLLAAGMAHEIGTPLAAIMGYSGILSEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
AGDPEKSDYLRRITEESKRIDRIVRGLLDYARPKGSQREEIEVLQLLEKVVELLEAQGAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
KLLEVTVEAEPSLPRLYADPHQLEQLLINLIMNAKDAMPKGGKLQLKGMSVGGGVAIEVA
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEE
DTGEGMPPEHLPLVFDPFFTTKEPGKGTGLGLAIAARIAESCGGTLTVQSELGEGSRFRL
CCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
TLPAGGSG
EECCCCCC
>Mature Secondary Structure
MKPTRFSLTFYTLSALSIMLVLTWVLLSVISFNTAEKDLLNQKGEEGKLLLSSIVLLLPR
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCC
PLAPPEPGSAAAKYLEHLRKDRAFVGLLVIDSAGRDLYTLSDPGGANAALLGVLRRGRGT
CCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCCE
FQVSPDGRLAWCYLPVMEQGRAIGAARLTLSLLPEREKLRGSRNLFLSYFILDFLLLLVV
EEECCCCCEEEEEECHHHCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHH
GSYLLSSSVVVPIRRLLAATRRIAAGDLSGTVDIPGSQEVAELGDAFNTMVVALREKREE
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
VEANVASLNRANREIIEARNEAIRSEKMASVGLLAAGMAHEIGTPLAAIMGYSGILSEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
AGDPEKSDYLRRITEESKRIDRIVRGLLDYARPKGSQREEIEVLQLLEKVVELLEAQGAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
KLLEVTVEAEPSLPRLYADPHQLEQLLINLIMNAKDAMPKGGKLQLKGMSVGGGVAIEVA
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEEEE
DTGEGMPPEHLPLVFDPFFTTKEPGKGTGLGLAIAARIAESCGGTLTVQSELGEGSRFRL
CCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEE
TLPAGGSG
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]