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The map label for this gene is pilD [H]

Identifier: 197117919

GI number: 197117919

Start: 1776793

End: 1777617

Strand: Direct

Name: pilD [H]

Synonym: Gbem_1532

Alternate gene names: 197117919

Gene position: 1776793-1777617 (Clockwise)

Preceding gene: 197117918

Following gene: 197117920

Centisome position: 38.5

GC content: 58.3

Gene sequence:

>825_bases
TTGAGCATCCCCTACATCATCTATTCCTGCTTCGCCTTCATCCTGGGGGGCGTGGTCGGCTCCTTCCTCAACGTCTGCAT
CTGCCGCATGCCCCAAGGGGAATCGGTGGTCTCCCCCCCTTCGCACTGCCCCAAGTGCGATTACCGCATCCGCTGGTACG
ATAACATCCCGGTGCTGAGCTATCTCCTCTTGAAGGGGAAATGCCGCGGCTGCGGCGCTCCCATCTCGGCGCAGTACCCG
CTGGTGGAGCTTTTGAACGCTTTCCTCACCCTGGCGCTTTTCATCAGGCTTTTCCCTGTGCGGCTCATCGAAGAGGCACC
GATGCAGATCGTGCTGAGACAGGGATCGATCTTTGGCGTCCTGTTCGTATTCTGCAGCGCGCTCGTCGTGGTCACCTTCA
TAGACCTGGAACACCAGATCATTCCCGACGCCATCACGCTCCCAGGCATCGTCGCCGGTTTTGCAGCGTCTTTTTTCATT
CCGCAACTGGGGTGGCTCAACTCGCTGATCGGCATATTGGCAGGCGGCGGGAGCCTCTTTCTTGTCGCCTATATCTACCA
GGCCATCGCCAAGAAGGACGGCATGGGAGGAGGAGACATCAAACTTCTCGCCATGATGGGCGCTTTCCTCGGCTGGAAGG
CAATCCTCTTCATCATCTTCACGGCCTCCCTGATCGGCTCCGTGATAGGGGTGAGCGTGATGCTGGCCCGTAAGGAGAAT
TCCAGCCTCGCCATCCCATTCGGCCCCTTCCTGGCAAGCGGCGCCGTGCTCTACATCTTCTACGGCAACAGAATCATTCA
CTGGTACCTGAGCATGGGAAGCTAA

Upstream 100 bases:

>100_bases
AAGTTTTTCTCCAGCCCCTGGCCCCTAGCCCCTAGCCCCGCTTTTAATTTTCATTTATACTGCTCTGCAGCCCGCTCATG
AGGGGCGTACGAGGTTCGCA

Downstream 100 bases:

>100_bases
AGCGTCCCGTATGAAACCTACCCGGTTCAGCCTCACCTTCTATACCCTCTCCGCCCTGAGCATCATGCTGGTGCTCACCT
GGGTGCTTCTTTCCGTAATC

Product: type IV prepilin-like proteins leader peptide processing enzyme

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSIPYIIYSCFAFILGGVVGSFLNVCICRMPQGESVVSPPSHCPKCDYRIRWYDNIPVLSYLLLKGKCRGCGAPISAQYP
LVELLNAFLTLALFIRLFPVRLIEEAPMQIVLRQGSIFGVLFVFCSALVVVTFIDLEHQIIPDAITLPGIVAGFAASFFI
PQLGWLNSLIGILAGGGSLFLVAYIYQAIAKKDGMGGGDIKLLAMMGAFLGWKAILFIIFTASLIGSVIGVSVMLARKEN
SSLAIPFGPFLASGAVLYIFYGNRIIHWYLSMGS

Sequences:

>Translated_274_residues
MSIPYIIYSCFAFILGGVVGSFLNVCICRMPQGESVVSPPSHCPKCDYRIRWYDNIPVLSYLLLKGKCRGCGAPISAQYP
LVELLNAFLTLALFIRLFPVRLIEEAPMQIVLRQGSIFGVLFVFCSALVVVTFIDLEHQIIPDAITLPGIVAGFAASFFI
PQLGWLNSLIGILAGGGSLFLVAYIYQAIAKKDGMGGGDIKLLAMMGAFLGWKAILFIIFTASLIGSVIGVSVMLARKEN
SSLAIPFGPFLASGAVLYIFYGNRIIHWYLSMGS
>Mature_273_residues
SIPYIIYSCFAFILGGVVGSFLNVCICRMPQGESVVSPPSHCPKCDYRIRWYDNIPVLSYLLLKGKCRGCGAPISAQYPL
VELLNAFLTLALFIRLFPVRLIEEAPMQIVLRQGSIFGVLFVFCSALVVVTFIDLEHQIIPDAITLPGIVAGFAASFFIP
QLGWLNSLIGILAGGGSLFLVAYIYQAIAKKDGMGGGDIKLLAMMGAFLGWKAILFIIFTASLIGSVIGVSVMLARKENS
SLAIPFGPFLASGAVLYIFYGNRIIHWYLSMGS

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=273, Percent_Identity=31.8681318681319, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789732, Length=147, Percent_Identity=36.0544217687075, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 29752; Mature: 29621

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPYIIYSCFAFILGGVVGSFLNVCICRMPQGESVVSPPSHCPKCDYRIRWYDNIPVLS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
YLLLKGKCRGCGAPISAQYPLVELLNAFLTLALFIRLFPVRLIEEAPMQIVLRQGSIFGV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
LFVFCSALVVVTFIDLEHQIIPDAITLPGIVAGFAASFFIPQLGWLNSLIGILAGGGSLF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LVAYIYQAIAKKDGMGGGDIKLLAMMGAFLGWKAILFIIFTASLIGSVIGVSVMLARKEN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SSLAIPFGPFLASGAVLYIFYGNRIIHWYLSMGS
CCEEECCHHHHHCCEEEEEEECCEEEEEEEECCC
>Mature Secondary Structure 
SIPYIIYSCFAFILGGVVGSFLNVCICRMPQGESVVSPPSHCPKCDYRIRWYDNIPVLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHH
YLLLKGKCRGCGAPISAQYPLVELLNAFLTLALFIRLFPVRLIEEAPMQIVLRQGSIFGV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHH
LFVFCSALVVVTFIDLEHQIIPDAITLPGIVAGFAASFFIPQLGWLNSLIGILAGGGSLF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LVAYIYQAIAKKDGMGGGDIKLLAMMGAFLGWKAILFIIFTASLIGSVIGVSVMLARKEN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
SSLAIPFGPFLASGAVLYIFYGNRIIHWYLSMGS
CCEEECCHHHHHCCEEEEEEECCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA