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The map label for this gene is folD-2 [H]

Identifier: 197117884

GI number: 197117884

Start: 1731891

End: 1732736

Strand: Direct

Name: folD-2 [H]

Synonym: Gbem_1497

Alternate gene names: 197117884

Gene position: 1731891-1732736 (Clockwise)

Preceding gene: 197117883

Following gene: 197117885

Centisome position: 37.53

GC content: 63.83

Gene sequence:

>846_bases
ATGGCTAAAATCATCGACGGCAAGGCCATCGCGGCGAAGATCCGTGCTGAAATCAGCGCGGAAGCGGCTCAACTGAAACA
AAAGGGGATCGTTCCGGGTCTCGCCGTGGTGCTGGTCGGAGAGGATCCCGCCAGCAAGGTCTATGTCTCCATGAAAGAAA
AGGCATGCGCCGACGTGGGGATTTTTTCCGACGAATACAAGCTTTCCGCCGACACCGGCGAGGCGGAGCTTCTGGCGCTG
ATCGACAAGCTGAACCACGACCCGAAGATCCACGGCATCCTGGTGCAGCTCCCGCTTCCCAAGCAGATCAACACCGAGCG
CGTGCTGGAGGCGATCTCCCCGGACAAGGACGCCGACGGCTTCCACCCCTATAACGTCGGGCGCCTGGTGATCGGCAAGC
CGCTCTTCCAGCCCTGCACCCCCTACGGCGTGATGGTGATGCTGAAGGAGGCGGGGGTCGAGCTTTCAGGGAAGGAAGTG
GTCGTGGTCGGCCGCTCCAACATCGTGGGCAAGCCCGTGGCCTTCATGTGCCTGCAGCAAAACGCCACCGTGACCCTGTG
CCATTCGAAGACCCGCGACCTGGCCGCGAAGGTGTCCCAGGCCGACGTGGTCATCGCCGCGGTCGGGGTGCCGGAGATGA
TCAAGGGGGCCTGGATCAAGGAGGGCGCCGTGGTGATCGACGTCGGCGTGAACCGGGTGGGCGAGAAGAAGCTTGTAGGG
GACGTGGAATACGCCGCGGCCAGCGAGCGCGCCTCGGCCATCACGCCGGTCCCCGGCGGCGTCGGCCCCATGACCATCAC
CATGCTGCTGCAGAACACGCTTGAGTCCGCCAAGAGGGGTCTCTAG

Upstream 100 bases:

>100_bases
TCCCGGTTCCTTATTATCTTGCTTTTAAAAAACTGTCTCAGGCGCCGGTCCCTATTACCTGTGCGCCTTATATTATTAAT
GAAACAAAGGGGTGGATGTA

Downstream 100 bases:

>100_bases
GGAAGTACTCCGCCCCCTGTCTCCAGACGGGGGGCGGGCCGCTTTGGCGGCAGCGGGGCGCCGTGCAATTCCATGTATTG
ACAAAGGAGTATCTCCATGC

Product: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAKIIDGKAIAAKIRAEISAEAAQLKQKGIVPGLAVVLVGEDPASKVYVSMKEKACADVGIFSDEYKLSADTGEAELLAL
IDKLNHDPKIHGILVQLPLPKQINTERVLEAISPDKDADGFHPYNVGRLVIGKPLFQPCTPYGVMVMLKEAGVELSGKEV
VVVGRSNIVGKPVAFMCLQQNATVTLCHSKTRDLAAKVSQADVVIAAVGVPEMIKGAWIKEGAVVIDVGVNRVGEKKLVG
DVEYAAASERASAITPVPGGVGPMTITMLLQNTLESAKRGL

Sequences:

>Translated_281_residues
MAKIIDGKAIAAKIRAEISAEAAQLKQKGIVPGLAVVLVGEDPASKVYVSMKEKACADVGIFSDEYKLSADTGEAELLAL
IDKLNHDPKIHGILVQLPLPKQINTERVLEAISPDKDADGFHPYNVGRLVIGKPLFQPCTPYGVMVMLKEAGVELSGKEV
VVVGRSNIVGKPVAFMCLQQNATVTLCHSKTRDLAAKVSQADVVIAAVGVPEMIKGAWIKEGAVVIDVGVNRVGEKKLVG
DVEYAAASERASAITPVPGGVGPMTITMLLQNTLESAKRGL
>Mature_280_residues
AKIIDGKAIAAKIRAEISAEAAQLKQKGIVPGLAVVLVGEDPASKVYVSMKEKACADVGIFSDEYKLSADTGEAELLALI
DKLNHDPKIHGILVQLPLPKQINTERVLEAISPDKDADGFHPYNVGRLVIGKPLFQPCTPYGVMVMLKEAGVELSGKEVV
VVGRSNIVGKPVAFMCLQQNATVTLCHSKTRDLAAKVSQADVVIAAVGVPEMIKGAWIKEGAVVIDVGVNRVGEKKLVGD
VEYAAASERASAITPVPGGVGPMTITMLLQNTLESAKRGL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=49.3150684931507, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=45.6081081081081, Blast_Score=221, Evalue=4e-58,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.8571428571429, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI36796743, Length=264, Percent_Identity=27.6515151515151, Blast_Score=94, Evalue=9e-20,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=53.6231884057971, Blast_Score=293, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=41.6382252559727, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=50.3496503496504, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=44.7811447811448, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6322933, Length=320, Percent_Identity=29.0625, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=48.1099656357388, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=48.1099656357388, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=48.1099656357388, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=48.1099656357388, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=45.4237288135593, Blast_Score=242, Evalue=2e-64,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=45.4237288135593, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29653; Mature: 29522

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIDGKAIAAKIRAEISAEAAQLKQKGIVPGLAVVLVGEDPASKVYVSMKEKACADVG
CCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCC
IFSDEYKLSADTGEAELLALIDKLNHDPKIHGILVQLPLPKQINTERVLEAISPDKDADG
CCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPYNVGRLVIGKPLFQPCTPYGVMVMLKEAGVELSGKEVVVVGRSNIVGKPVAFMCLQQ
CCCCCCCEEEECCCCCCCCCCHHEEEEEECCCCEECCCEEEEECCCCCCCCCCEEEEEEC
NATVTLCHSKTRDLAAKVSQADVVIAAVGVPEMIKGAWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHCCEEECCCEEEEECCCCCCCHHHCC
DVEYAAASERASAITPVPGGVGPMTITMLLQNTLESAKRGL
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIIDGKAIAAKIRAEISAEAAQLKQKGIVPGLAVVLVGEDPASKVYVSMKEKACADVG
CEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEHHHHHHHCC
IFSDEYKLSADTGEAELLALIDKLNHDPKIHGILVQLPLPKQINTERVLEAISPDKDADG
CCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPYNVGRLVIGKPLFQPCTPYGVMVMLKEAGVELSGKEVVVVGRSNIVGKPVAFMCLQQ
CCCCCCCEEEECCCCCCCCCCHHEEEEEECCCCEECCCEEEEECCCCCCCCCCEEEEEEC
NATVTLCHSKTRDLAAKVSQADVVIAAVGVPEMIKGAWIKEGAVVIDVGVNRVGEKKLVG
CCEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHCCEEECCCEEEEECCCCCCCHHHCC
DVEYAAASERASAITPVPGGVGPMTITMLLQNTLESAKRGL
CHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA