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The map label for this gene is ptxB [H]

Identifier: 197117874

GI number: 197117874

Start: 1725372

End: 1726298

Strand: Reverse

Name: ptxB [H]

Synonym: Gbem_1487

Alternate gene names: 197117874

Gene position: 1726298-1725372 (Counterclockwise)

Preceding gene: 308535243

Following gene: 197117873

Centisome position: 37.41

GC content: 57.5

Gene sequence:

>927_bases
GTGCTGACCAACCGGTTCATAGCTGTACTTATGGCTCTGGCTGTCATCATAGTCCCAGGATGCAGCAAAGAGGAACCGAA
GAAGATCGATCTGGGCAAAAGGGCGGTCCTCAAAAGCGAAGACAGGGCGAAGACGGGGGTGATCCGCATCGCGGTAGGCG
GGATGATAACTCCCAAGGAGGGACTCGCCTACTACCGCGAGTTCCTCGACTACATCGGCAACGAGCTCGGCAGGCCGGTC
GAATACGTGGACTCGCCGAACTACGAGGAAACCAACAAGATGCTGGAGAACCGGGAACTCGACGCGGCCATCGTCTGCAG
CGGCCCCTATGTCGACGGCCATAAGAAGTTCGGCCTGGAACTTCTGGCAGCACCCAAAGCCTTCGGGGCTCCCGTTTATT
ATTCCTACATCATCGTCCCCAAGGAGAGTTCCGCCAGAGCTCTGGACGACCTGAAAGGAAAGTCGTTCGCTTTCACGGAT
CCTCTCTCCAACACGGGGAAGTTGGTGCCCGACTACCTCCTCGGGAAGAAGGGGTACAACTCGAACACCTTCTTCAGCAA
GACCGTCTATTCGGGCTCCCACGACAAGTCCATACAGGCCGTGGCCGGTGCCCTCGTCGACGGAGCTGCGGTGGACAGTG
TCATCTGGGAATACCTGGCCAAGACAAAGCCCGAACTGACGGGTAAGACCCGGATCGTCCTCAGGTCCGAGCCTTACGCC
ATCCCGCCGTTCGTGGTCCATCCCGCACTGGATCCCGCAGTGAAGAAAAAACTCCGCACCATACTGCTCACCGCCCACAC
CAACCAAAGAGGGAAGGTAATCCTGGACAAGATGATGACCGAGAGCTATGTCCCCATAGAGGACAAGGCCTATGACTCGG
TGCGCAACATGAAGAAATGGTTGGCCGGCGAGCAAAAGGAGCGATAG

Upstream 100 bases:

>100_bases
ATGCAGTGCAGATTTCAAGTTCTCTTTAAAAAGAACCGATAACTGTATGTAGCTGATCCACAACCTGTCTCGGGCAACCC
CATGTCAGGAGGTCCCTATC

Downstream 100 bases:

>100_bases
TCAAGGCATGGGAAAACGCGCCCTCAACCTGAGATCGAAGATCCTGGCCATATTCCTGACCCTCTCCATGGCGACCATAG
CCGGCATGGTCGTGATCGCC

Product: ABC transporter substrate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLTNRFIAVLMALAVIIVPGCSKEEPKKIDLGKRAVLKSEDRAKTGVIRIAVGGMITPKEGLAYYREFLDYIGNELGRPV
EYVDSPNYEETNKMLENRELDAAIVCSGPYVDGHKKFGLELLAAPKAFGAPVYYSYIIVPKESSARALDDLKGKSFAFTD
PLSNTGKLVPDYLLGKKGYNSNTFFSKTVYSGSHDKSIQAVAGALVDGAAVDSVIWEYLAKTKPELTGKTRIVLRSEPYA
IPPFVVHPALDPAVKKKLRTILLTAHTNQRGKVILDKMMTESYVPIEDKAYDSVRNMKKWLAGEQKER

Sequences:

>Translated_308_residues
MLTNRFIAVLMALAVIIVPGCSKEEPKKIDLGKRAVLKSEDRAKTGVIRIAVGGMITPKEGLAYYREFLDYIGNELGRPV
EYVDSPNYEETNKMLENRELDAAIVCSGPYVDGHKKFGLELLAAPKAFGAPVYYSYIIVPKESSARALDDLKGKSFAFTD
PLSNTGKLVPDYLLGKKGYNSNTFFSKTVYSGSHDKSIQAVAGALVDGAAVDSVIWEYLAKTKPELTGKTRIVLRSEPYA
IPPFVVHPALDPAVKKKLRTILLTAHTNQRGKVILDKMMTESYVPIEDKAYDSVRNMKKWLAGEQKER
>Mature_308_residues
MLTNRFIAVLMALAVIIVPGCSKEEPKKIDLGKRAVLKSEDRAKTGVIRIAVGGMITPKEGLAYYREFLDYIGNELGRPV
EYVDSPNYEETNKMLENRELDAAIVCSGPYVDGHKKFGLELLAAPKAFGAPVYYSYIIVPKESSARALDDLKGKSFAFTD
PLSNTGKLVPDYLLGKKGYNSNTFFSKTVYSGSHDKSIQAVAGALVDGAAVDSVIWEYLAKTKPELTGKTRIVLRSEPYA
IPPFVVHPALDPAVKKKLRTILLTAHTNQRGKVILDKMMTESYVPIEDKAYDSVRNMKKWLAGEQKER

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=274, Percent_Identity=22.992700729927, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 34098; Mature: 34098

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNRFIAVLMALAVIIVPGCSKEEPKKIDLGKRAVLKSEDRAKTGVIRIAVGGMITPKE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHCEEEEEECCCCCCHH
GLAYYREFLDYIGNELGRPVEYVDSPNYEETNKMLENRELDAAIVCSGPYVDGHKKFGLE
HHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCEE
LLAAPKAFGAPVYYSYIIVPKESSARALDDLKGKSFAFTDPLSNTGKLVPDYLLGKKGYN
EEECCHHHCCCEEEEEEEEECCCCHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCCCCC
SNTFFSKTVYSGSHDKSIQAVAGALVDGAAVDSVIWEYLAKTKPELTGKTRIVLRSEPYA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
IPPFVVHPALDPAVKKKLRTILLTAHTNQRGKVILDKMMTESYVPIEDKAYDSVRNMKKW
CCCEEECCCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LAGEQKER
HCCCCCCC
>Mature Secondary Structure
MLTNRFIAVLMALAVIIVPGCSKEEPKKIDLGKRAVLKSEDRAKTGVIRIAVGGMITPKE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCHHHCEEEEEECCCCCCHH
GLAYYREFLDYIGNELGRPVEYVDSPNYEETNKMLENRELDAAIVCSGPYVDGHKKFGLE
HHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCEE
LLAAPKAFGAPVYYSYIIVPKESSARALDDLKGKSFAFTDPLSNTGKLVPDYLLGKKGYN
EEECCHHHCCCEEEEEEEEECCCCHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCCCCC
SNTFFSKTVYSGSHDKSIQAVAGALVDGAAVDSVIWEYLAKTKPELTGKTRIVLRSEPYA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCC
IPPFVVHPALDPAVKKKLRTILLTAHTNQRGKVILDKMMTESYVPIEDKAYDSVRNMKKW
CCCEEECCCCCHHHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
LAGEQKER
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]