BacMap

Definition	Geobacter bemidjiensis Bem chromosome, complete genome.
Accession	NC_011146
Length	4,615,150

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The map label for this gene is paaF [C]

Identifier: 197117849

GI number: 197117849

Start: 1691732

End: 1692511

Strand: Direct

Name: paaF [C]

Synonym: Gbem_1462

Alternate gene names: 197117849

Gene position: 1691732-1692511 (Clockwise)

Preceding gene: 197117848

Following gene: 197117850

Centisome position: 36.66

GC content: 63.59

Gene sequence:

>780_bases
ATGTACGAAGATCTGCTGCTGGAAAAAAAGGACGGGATCGCGCTGCTTCAGATCAACCGTCCCAAGGCGATGAACAGCCT
GAACGACGCCGTGCTGGACCAGTTGCTGCACGCCTTTGAGGTGCTCGTCCTGGACCGCGAGGTGCGGGTGGTGGTCTTGA
CCGGTGCGGGAGAGAAGGCCTTCGTCGCCGGGGCCGACATCGCCGAGATGAAGAGCCTCAACGTGGAGCAGGCGCTCGCC
TTCTCCCGCAAGGGACAGCAGCTGGTGCAGCTGATCGGGAAGGTCCCCAAGCCGGTGATCGCCGCCGTGAACGGCTTCGC
GCTCGGGGGGGGGCTTGAGCTGGCGATGGCCTGCGATTTCGCCTACGCCGCGGAGAAAACGAAGATCGGCCTCCCCGAGG
TCACCCTGGGTATCATCCCGGGTTTCGGCGGCACCCAAAGCATGGCGCGCCTGATTGGCCGCAGCCGGGCCAACGAGCTG
ATCTTCTCCGGCAGGCTGATCACAGCCGCCGAGGCGAAGAACTGGGGGCTATTCTGCGCCGTATTCCCGGCTCAAAACCT
CATGGCCGAGGTCATGGCGACCGCCGCCCAGATCGCCGGCAACTCCCGCCTGGGTGTGGCCCATGCAAAAGACGCGGTGA
AAAGCGGGCTGGAGATGAGCGTCGCCGAAGGGATGGGGTACGAGGCTTTGCACTTCGCCTCGCTCTTCGCCACTTTAGAC
CAGAAGGAAGGGATGACCGCCTTCCTCGAAAAGAGAAAACCCGCCTTCGTCGGAGCTTGA

Upstream 100 bases:

>100_bases
GTACCGTCCCTGCCCGCTGCTGGCCAAGATGGTCGAAGCGGGCTACCTGGGCAAAAAGAGCGGCAGAGGGTTCTACAACT
ACGCGTAAAAGAGGTGTGCC

Downstream 100 bases:

>100_bases
GGCAGCCAACGCCGCTTCCACATCCCTCACCCCCGCCCCTCTCCCAGAGGGCGAGGGGAGAATCTCGATGAACCGACCGT
CGTCCATCCCCTCTCCCTCT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MYEDLLLEKKDGIALLQINRPKAMNSLNDAVLDQLLHAFEVLVLDREVRVVVLTGAGEKAFVAGADIAEMKSLNVEQALA
FSRKGQQLVQLIGKVPKPVIAAVNGFALGGGLELAMACDFAYAAEKTKIGLPEVTLGIIPGFGGTQSMARLIGRSRANEL
IFSGRLITAAEAKNWGLFCAVFPAQNLMAEVMATAAQIAGNSRLGVAHAKDAVKSGLEMSVAEGMGYEALHFASLFATLD
QKEGMTAFLEKRKPAFVGA

Sequences:

>Translated_259_residues
MYEDLLLEKKDGIALLQINRPKAMNSLNDAVLDQLLHAFEVLVLDREVRVVVLTGAGEKAFVAGADIAEMKSLNVEQALA
FSRKGQQLVQLIGKVPKPVIAAVNGFALGGGLELAMACDFAYAAEKTKIGLPEVTLGIIPGFGGTQSMARLIGRSRANEL
IFSGRLITAAEAKNWGLFCAVFPAQNLMAEVMATAAQIAGNSRLGVAHAKDAVKSGLEMSVAEGMGYEALHFASLFATLD
QKEGMTAFLEKRKPAFVGA
>Mature_259_residues
MYEDLLLEKKDGIALLQINRPKAMNSLNDAVLDQLLHAFEVLVLDREVRVVVLTGAGEKAFVAGADIAEMKSLNVEQALA
FSRKGQQLVQLIGKVPKPVIAAVNGFALGGGLELAMACDFAYAAEKTKIGLPEVTLGIIPGFGGTQSMARLIGRSRANEL
IFSGRLITAAEAKNWGLFCAVFPAQNLMAEVMATAAQIAGNSRLGVAHAKDAVKSGLEMSVAEGMGYEALHFASLFATLD
QKEGMTAFLEKRKPAFVGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=45.7364341085271, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=37.984496124031, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=32.3308270676692, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=32.1428571428571, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI20127408, Length=172, Percent_Identity=34.3023255813954, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=31.2, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI68989263, Length=171, Percent_Identity=37.4269005847953, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI37594469, Length=155, Percent_Identity=33.5483870967742, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI37594471, Length=155, Percent_Identity=33.5483870967742, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI221136756, Length=256, Percent_Identity=25.78125, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI221136753, Length=256, Percent_Identity=25.78125, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI221307494, Length=256, Percent_Identity=25.78125, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI25453481, Length=260, Percent_Identity=23.8461538461538, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI51538573, Length=260, Percent_Identity=23.8461538461538, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4757968, Length=260, Percent_Identity=23.8461538461538, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI49355787, Length=260, Percent_Identity=23.8461538461538, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4757966, Length=249, Percent_Identity=24.0963855421687, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51538581, Length=249, Percent_Identity=24.0963855421687, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=196, Percent_Identity=28.0612244897959, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260275230, Length=196, Percent_Identity=28.0612244897959, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI116235487, Length=256, Percent_Identity=23.828125, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI260274832, Length=196, Percent_Identity=28.0612244897959, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=182, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI157694516, Length=182, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI62530384, Length=248, Percent_Identity=25.4032258064516, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=75, Percent_Identity=50.6666666666667, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=40.7843137254902, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=34.7169811320755, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=40.6091370558376, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1788597, Length=270, Percent_Identity=31.4814814814815, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=30.5555555555556, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=32.8301886792453, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=37.696335078534, Blast_Score=110, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=248, Percent_Identity=47.9838709677419, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=44.1767068273092, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=34.8017621145374, Blast_Score=148, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=28.957528957529, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=36, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=31.5789473684211, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=220, Percent_Identity=34.0909090909091, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17508951, Length=220, Percent_Identity=34.0909090909091, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=220, Percent_Identity=34.0909090909091, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=24.9056603773585, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI25144160, Length=158, Percent_Identity=31.0126582278481, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=158, Percent_Identity=31.0126582278481, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=176, Percent_Identity=27.8409090909091, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=195, Percent_Identity=26.1538461538462, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=247, Percent_Identity=42.914979757085, Blast_Score=200, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24653477, Length=247, Percent_Identity=42.914979757085, Blast_Score=200, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=39.7727272727273, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=28.6274509803922, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920382, Length=258, Percent_Identity=30.6201550387597, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=28.2352941176471, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24654903, Length=220, Percent_Identity=26.8181818181818, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24583077, Length=204, Percent_Identity=34.3137254901961, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24583079, Length=204, Percent_Identity=34.3137254901961, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19921000, Length=204, Percent_Identity=34.3137254901961, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24650670, Length=210, Percent_Identity=30.4761904761905, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571729, Length=141, Percent_Identity=29.0780141843972, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571730, Length=141, Percent_Identity=29.0780141843972, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921018, Length=172, Percent_Identity=32.5581395348837, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24583165, Length=168, Percent_Identity=30.952380952381, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27479; Mature: 27479

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEDLLLEKKDGIALLQINRPKAMNSLNDAVLDQLLHAFEVLVLDREVRVVVLTGAGEKA
CCCHHCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
FVAGADIAEMKSLNVEQALAFSRKGQQLVQLIGKVPKPVIAAVNGFALGGGLELAMACDF
EEECCCHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHCCEEECCCCEEEHHHHH
AYAAEKTKIGLPEVTLGIIPGFGGTQSMARLIGRSRANELIFSGRLITAAEAKNWGLFCA
HHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCEEEE
VFPAQNLMAEVMATAAQIAGNSRLGVAHAKDAVKSGLEMSVAEGMGYEALHFASLFATLD
ECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHH
QKEGMTAFLEKRKPAFVGA
HCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYEDLLLEKKDGIALLQINRPKAMNSLNDAVLDQLLHAFEVLVLDREVRVVVLTGAGEKA
CCCHHCEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEE
FVAGADIAEMKSLNVEQALAFSRKGQQLVQLIGKVPKPVIAAVNGFALGGGLELAMACDF
EEECCCHHHHHHCCHHHHHHHHHCHHHHHHHHHCCCCHHHHHHCCEEECCCCEEEHHHHH
AYAAEKTKIGLPEVTLGIIPGFGGTQSMARLIGRSRANELIFSGRLITAAEAKNWGLFCA
HHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCEEEE
VFPAQNLMAEVMATAAQIAGNSRLGVAHAKDAVKSGLEMSVAEGMGYEALHFASLFATLD
ECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHH
QKEGMTAFLEKRKPAFVGA
HCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA