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The map label for this gene is moaA-2 [H]

Identifier: 197117823

GI number: 197117823

Start: 1658590

End: 1659570

Strand: Direct

Name: moaA-2 [H]

Synonym: Gbem_1436

Alternate gene names: 197117823

Gene position: 1658590-1659570 (Clockwise)

Preceding gene: 197117822

Following gene: 197117824

Centisome position: 35.94

GC content: 64.73

Gene sequence:

>981_bases
GTGTCACTCATCGACGCTCACGGCAGGCGCATCAACTACCTTCGGCTCTCCGTGACCGACCGTTGCAACCTCCGCTGCAG
TTACTGCATGCCGGCCCAGGGTGTAAAGCTCCTGCCCCAGGACCGGGTGCTCTCCTACGAGGAGCTCTTGCGGGTCGCGA
AGCAAGCGGTCGCCGTCGGGATAGAGAAGATCCGGGTGACCGGCGGCGAGCCCCTGGTGAGAAAGGGGATTGTCCCCTTC
CTTAAACGGTTGGCGCTGATACCGGGGCTCGAAGAGCTGGTGCTGACCACCAACGGAACACTCCTGCAGGGAATGGCCCG
GGAGCTTAAAGATGCCGGCGTTCAGCGCCTGAATATCAGCCTCGACTCCCTGAGCCCTGTGACCTTCGCCAAGATCACCC
GCGGCGGCGATCTCAAGGCGGCGCTCGCGGGGCTTGAGGCGGCCGAGCGGGCCGGTTTCCCGCCGCACAAGATAAACGTG
GTGCTGATGCGCGGGGTCAACGACCACGAAATCCTCGATTTCGTGCGGCTCACACTGGAGCGCCCCTACGCGGTGAGGTT
CATCGAGTACATGCCTACCTGCCAAGCGGCCGACTGGCGTTCGCTCTGCATTCCGGGGAGCGAGGTGCTGGAGCGGATCG
CGGAGCGTTTCCTGATCGAGAAGCTGGAGCGAAACGAGCGTTCGGGACCGGCCAAGAACTTCAGAGTTAAGGGCGCTGTC
GGCTCGCTCGGGGTCATCACCCCGATGACCGGTCACTTCTGCGACGCCTGCAACCGGCTCAGGGTGACCGCCACAGGCGT
GGCCAAGGGGTGCCTGTTGTCCGGGGCAGGGGTGGATCTAGGACCGCTTCTGGCCTCCGGCGACGATGCCTCGCTACGCG
ACGGGATCCGCCGCATCGTCCACGACAAGCCAGGCCGTCACCGGTTCGACGAGGAAGCCGGCGTCGTAGCGCCCTTCGTC
ATGGCAGGGATAGGGGGATAG

Upstream 100 bases:

>100_bases
GCTGCCGCTGCCGCCGGAAGGTGTTGCGGCGATACTGGGCGGCAACGCGGCCAAACTTTTGAACCTGGGGTGACGCCGCC
TCGCGGCAAAGGAGGAGATC

Downstream 100 bases:

>100_bases
GATGGCTTCTGTTTATCGTTACGAAAAAGGGGCCGTTACCGCAGCTGAGAGGGAAGTGGTGGCCGAGGTCCCCGTGGTTC
TGCACGTGAACGGCAGGGAG

Product: pyranopterin triphosphate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSLIDAHGRRINYLRLSVTDRCNLRCSYCMPAQGVKLLPQDRVLSYEELLRVAKQAVAVGIEKIRVTGGEPLVRKGIVPF
LKRLALIPGLEELVLTTNGTLLQGMARELKDAGVQRLNISLDSLSPVTFAKITRGGDLKAALAGLEAAERAGFPPHKINV
VLMRGVNDHEILDFVRLTLERPYAVRFIEYMPTCQAADWRSLCIPGSEVLERIAERFLIEKLERNERSGPAKNFRVKGAV
GSLGVITPMTGHFCDACNRLRVTATGVAKGCLLSGAGVDLGPLLASGDDASLRDGIRRIVHDKPGRHRFDEEAGVVAPFV
MAGIGG

Sequences:

>Translated_326_residues
MSLIDAHGRRINYLRLSVTDRCNLRCSYCMPAQGVKLLPQDRVLSYEELLRVAKQAVAVGIEKIRVTGGEPLVRKGIVPF
LKRLALIPGLEELVLTTNGTLLQGMARELKDAGVQRLNISLDSLSPVTFAKITRGGDLKAALAGLEAAERAGFPPHKINV
VLMRGVNDHEILDFVRLTLERPYAVRFIEYMPTCQAADWRSLCIPGSEVLERIAERFLIEKLERNERSGPAKNFRVKGAV
GSLGVITPMTGHFCDACNRLRVTATGVAKGCLLSGAGVDLGPLLASGDDASLRDGIRRIVHDKPGRHRFDEEAGVVAPFV
MAGIGG
>Mature_325_residues
SLIDAHGRRINYLRLSVTDRCNLRCSYCMPAQGVKLLPQDRVLSYEELLRVAKQAVAVGIEKIRVTGGEPLVRKGIVPFL
KRLALIPGLEELVLTTNGTLLQGMARELKDAGVQRLNISLDSLSPVTFAKITRGGDLKAALAGLEAAERAGFPPHKINVV
LMRGVNDHEILDFVRLTLERPYAVRFIEYMPTCQAADWRSLCIPGSEVLERIAERFLIEKLERNERSGPAKNFRVKGAVG
SLGVITPMTGHFCDACNRLRVTATGVAKGCLLSGAGVDLGPLLASGDDASLRDGIRRIVHDKPGRHRFDEEAGVVAPFVM
AGIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=288, Percent_Identity=42.0138888888889, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI22027536, Length=288, Percent_Identity=42.0138888888889, Blast_Score=222, Evalue=4e-58,
Organism=Escherichia coli, GI1786999, Length=286, Percent_Identity=37.0629370629371, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI32566331, Length=307, Percent_Identity=40.0651465798046, Blast_Score=231, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI32566329, Length=307, Percent_Identity=40.0651465798046, Blast_Score=230, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17561210, Length=309, Percent_Identity=39.1585760517799, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI28574490, Length=295, Percent_Identity=43.728813559322, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI28574488, Length=295, Percent_Identity=43.728813559322, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35469; Mature: 35338

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIDAHGRRINYLRLSVTDRCNLRCSYCMPAQGVKLLPQDRVLSYEELLRVAKQAVAVG
CCCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCCEECCHHHCCCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLKRLALIPGLEELVLTTNGTLLQGMARELKDAGVQRLNIS
HHHEEECCCCHHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHCCCEEEEEE
LDSLSPVTFAKITRGGDLKAALAGLEAAERAGFPPHKINVVLMRGVNDHEILDFVRLTLE
CCCCCCCHHEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHC
RPYAVRFIEYMPTCQAADWRSLCIPGSEVLERIAERFLIEKLERNERSGPAKNFRVKGAV
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
GSLGVITPMTGHFCDACNRLRVTATGVAKGCLLSGAGVDLGPLLASGDDASLRDGIRRIV
CCCCCCCCCCHHHHHHHCCEEEEECCHHHHHEEECCCCCCCCCEECCCCHHHHHHHHHHH
HDKPGRHRFDEEAGVVAPFVMAGIGG
CCCCCCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SLIDAHGRRINYLRLSVTDRCNLRCSYCMPAQGVKLLPQDRVLSYEELLRVAKQAVAVG
CCCCCCCCEEEEEEEEECCCCCCEEEECCCCCCCEECCHHHCCCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLKRLALIPGLEELVLTTNGTLLQGMARELKDAGVQRLNIS
HHHEEECCCCHHHHHHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHCCCEEEEEE
LDSLSPVTFAKITRGGDLKAALAGLEAAERAGFPPHKINVVLMRGVNDHEILDFVRLTLE
CCCCCCCHHEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHC
RPYAVRFIEYMPTCQAADWRSLCIPGSEVLERIAERFLIEKLERNERSGPAKNFRVKGAV
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
GSLGVITPMTGHFCDACNRLRVTATGVAKGCLLSGAGVDLGPLLASGDDASLRDGIRRIV
CCCCCCCCCCHHHHHHHCCEEEEECCHHHHHEEECCCCCCCCCEECCCCHHHHHHHHHHH
HDKPGRHRFDEEAGVVAPFVMAGIGG
CCCCCCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA