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The map label for this gene is fhlA [H]

Identifier: 197117799

GI number: 197117799

Start: 1625118

End: 1627112

Strand: Direct

Name: fhlA [H]

Synonym: Gbem_1411

Alternate gene names: 197117799

Gene position: 1625118-1627112 (Clockwise)

Preceding gene: 197117798

Following gene: 197117800

Centisome position: 35.21

GC content: 57.59

Gene sequence:

>1995_bases
ATGGCCTTGTCGAGCGATCCTGCTGCGGAGTTTTTCCTAGGTAAATTCAGCAATTTGGAACAGCTGTTCGGTCAGGCCCA
GTTCGGTGTCTCCATCATAGACTGCGACCTGCGCTACATCTACGTCAACGACCGGATGGCGTCGATCAACGGCGTGCCCC
CAAAAGAGCATCTGGGGAAGACCGTAAGGGAGGTCGACCGCTTCGTCGCTCTGGTGGTGGAGCCGTTGCTGAACCGGACC
ATCCATGAGGATCATTCCTTCGTGGACCTTGAGATACGGCTGTCGAAACCGGGCGACCCTCAGGAGAAATCCTGGTTGAT
CTGCTCTTATCCCCTCAAGGAAAGCGACGGTTCCGTGACCCGCTGCGGCCTGGTGGTTCAGGATATAACCGAGCAGAAGC
TAAGGCAGAGCCTGCAGACAGAGCGGCTGCAGGTTGAGGCCTTCCTCTCAAACCTCTCTTCCGCCTTCATCAACGTGCCG
GTGAGCGAGGTGGATCGAAAGATAGAAAAGGCATTGCAAAAGGTCGTCAACTTCCTCGGTTTCGAAAGAAGCAGCATCTG
GCACTTCACCCCTGATCGCAGTAGTTTGCGCATCACTCATTCCTACGCGCTCCCCGGCGTCAAGCCACCCCCCACAGAAT
TGATGAACCTGATCCCGATATGGATCGAGATGATCCAGCTTGGGGAGAGCTTCCGCGTCTCCGACGTTGAGGAGCTGTCG
GACAAGTTTTGGAGGGAAAAACAATACTGCAAGGACCAGGGCGGCATCAAGTCCATCATGTTCATTCCCGCCAGTGTCGG
CGGCACCATAGTCGGCGCCATCACCTTCGTTTCCTACAGCGTCAAGAAAGAGTGGCCCGACGAGCTGACTCAGAGGCTCC
GCCTTTTATGGGAAGTTTTCGCCAACGCCCTCGAGCGTAAGAGGGCCGATCAGAAAATCCAGAACGCCCTGGCGGAAATA
CGACAGCTGAAAGACCGCCTGGAGGCGGAAAACGTCTATCTGCGCGACCAGATCGACGTGGAATATAAGCACGAGGAGAT
CATCGGCAAATCGGAGGCGATAAGAAACGTGCTGCAGCAGATACAGCAGGTGGCGCCAACCGATTCCACTGTGCTCATCC
TGGGCGAAACCGGCACCGGCAAGGAACTGATAGCGAGGGCGATCCACAACGCCGGCCAGCGCAAGGCACGCGCCATGATC
AAGGTGAACTGCGCCGCATTGCCGGCAGCCCTCATCGAGGCGGAGCTGTTCGGTCACGAAAAAGGGGCCTATACCGGAGC
CGTATCCTCCCAGATCGGGCGCTTCGAGGCGGCTAACGGATCGACCATCTTCCTGGACGAGATAGGGGAGCTCCCCCTGG
AGCTGCAGTCGAAGCTGCTGCGGGTCATCCAGGAAGGGCAATTCGAGCGCCTGGGAAATTCGAAGCCGGTCAAGGCGGAT
GTGAGGGTGATCGCCGCGACCAACGTGAGCCTGAAGCAGGCAGTGAAGGAAGGAAAATTCCGGGAGGACCTATATTACAG
GCTCAACGTCTTCCCTATTTTCGTCCCCCCGCTGCGCGACCGGCAGGAGGACATACCGCTGTTGGTATGGGCGATGGTGG
AGGAGTTCTCCAAGGTTTTCGGCAAGACCATCGAGCGCATTCCCAGGAAAAACATGGAGATCATGGAGCGCTACTTGTGG
CCGGGAAACATAAGGGAGCTGAGAAACCTGGTGGAACGGGCCATGATCCTCAGTAACGGCGGCACCCTGGTGGTGGACAT
TCTGGATGGATCCGCCGCGGCCGCAAACGAGCCGATGACGATGGAGGCCGCGGAAAGAAAGCATATAGGCTTCATACTGG
AAAAGACGGGGTGGCGCATCCGCGGCAAGGGTGGCGCGGCCGAAATTCTCGACATGAAACCCACCACGCTGCATTCCCGG
ATCAAGAAACTCGGCGTCAAAAGGAAGAACGGAACGCTCGAACCGCCCCCCGCTGCAGAGAAACCAACTTCCTGA

Upstream 100 bases:

>100_bases
TCCGGATTCCCTTAATGTTCCTGTCGTTCGTTGATGCATATTTTAAAACGATGTGCTATTATCCTGTACACATTTTTATT
TTAATGAATACGGTGACGCC

Downstream 100 bases:

>100_bases
TCCCTGTCTCACCCACCTGGTCCGCAGCGTAAAGGTGGCACCTGCCCCCACCCCCTGACCCCCTCCCGCAAGGGGAGGGG
GGAATAAGCTCCTTCACCCC

Product: sigma-54-dependent sensor transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MALSSDPAAEFFLGKFSNLEQLFGQAQFGVSIIDCDLRYIYVNDRMASINGVPPKEHLGKTVREVDRFVALVVEPLLNRT
IHEDHSFVDLEIRLSKPGDPQEKSWLICSYPLKESDGSVTRCGLVVQDITEQKLRQSLQTERLQVEAFLSNLSSAFINVP
VSEVDRKIEKALQKVVNFLGFERSSIWHFTPDRSSLRITHSYALPGVKPPPTELMNLIPIWIEMIQLGESFRVSDVEELS
DKFWREKQYCKDQGGIKSIMFIPASVGGTIVGAITFVSYSVKKEWPDELTQRLRLLWEVFANALERKRADQKIQNALAEI
RQLKDRLEAENVYLRDQIDVEYKHEEIIGKSEAIRNVLQQIQQVAPTDSTVLILGETGTGKELIARAIHNAGQRKARAMI
KVNCAALPAALIEAELFGHEKGAYTGAVSSQIGRFEAANGSTIFLDEIGELPLELQSKLLRVIQEGQFERLGNSKPVKAD
VRVIAATNVSLKQAVKEGKFREDLYYRLNVFPIFVPPLRDRQEDIPLLVWAMVEEFSKVFGKTIERIPRKNMEIMERYLW
PGNIRELRNLVERAMILSNGGTLVVDILDGSAAAANEPMTMEAAERKHIGFILEKTGWRIRGKGGAAEILDMKPTTLHSR
IKKLGVKRKNGTLEPPPAAEKPTS

Sequences:

>Translated_664_residues
MALSSDPAAEFFLGKFSNLEQLFGQAQFGVSIIDCDLRYIYVNDRMASINGVPPKEHLGKTVREVDRFVALVVEPLLNRT
IHEDHSFVDLEIRLSKPGDPQEKSWLICSYPLKESDGSVTRCGLVVQDITEQKLRQSLQTERLQVEAFLSNLSSAFINVP
VSEVDRKIEKALQKVVNFLGFERSSIWHFTPDRSSLRITHSYALPGVKPPPTELMNLIPIWIEMIQLGESFRVSDVEELS
DKFWREKQYCKDQGGIKSIMFIPASVGGTIVGAITFVSYSVKKEWPDELTQRLRLLWEVFANALERKRADQKIQNALAEI
RQLKDRLEAENVYLRDQIDVEYKHEEIIGKSEAIRNVLQQIQQVAPTDSTVLILGETGTGKELIARAIHNAGQRKARAMI
KVNCAALPAALIEAELFGHEKGAYTGAVSSQIGRFEAANGSTIFLDEIGELPLELQSKLLRVIQEGQFERLGNSKPVKAD
VRVIAATNVSLKQAVKEGKFREDLYYRLNVFPIFVPPLRDRQEDIPLLVWAMVEEFSKVFGKTIERIPRKNMEIMERYLW
PGNIRELRNLVERAMILSNGGTLVVDILDGSAAAANEPMTMEAAERKHIGFILEKTGWRIRGKGGAAEILDMKPTTLHSR
IKKLGVKRKNGTLEPPPAAEKPTS
>Mature_663_residues
ALSSDPAAEFFLGKFSNLEQLFGQAQFGVSIIDCDLRYIYVNDRMASINGVPPKEHLGKTVREVDRFVALVVEPLLNRTI
HEDHSFVDLEIRLSKPGDPQEKSWLICSYPLKESDGSVTRCGLVVQDITEQKLRQSLQTERLQVEAFLSNLSSAFINVPV
SEVDRKIEKALQKVVNFLGFERSSIWHFTPDRSSLRITHSYALPGVKPPPTELMNLIPIWIEMIQLGESFRVSDVEELSD
KFWREKQYCKDQGGIKSIMFIPASVGGTIVGAITFVSYSVKKEWPDELTQRLRLLWEVFANALERKRADQKIQNALAEIR
QLKDRLEAENVYLRDQIDVEYKHEEIIGKSEAIRNVLQQIQQVAPTDSTVLILGETGTGKELIARAIHNAGQRKARAMIK
VNCAALPAALIEAELFGHEKGAYTGAVSSQIGRFEAANGSTIFLDEIGELPLELQSKLLRVIQEGQFERLGNSKPVKADV
RVIAATNVSLKQAVKEGKFREDLYYRLNVFPIFVPPLRDRQEDIPLLVWAMVEEFSKVFGKTIERIPRKNMEIMERYLWP
GNIRELRNLVERAMILSNGGTLVVDILDGSAAAANEPMTMEAAERKHIGFILEKTGWRIRGKGGAAEILDMKPTTLHSRI
KKLGVKRKNGTLEPPPAAEKPTS

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=405, Percent_Identity=47.1604938271605, Blast_Score=347, Evalue=2e-96,
Organism=Escherichia coli, GI87082117, Length=547, Percent_Identity=37.1115173674589, Blast_Score=320, Evalue=3e-88,
Organism=Escherichia coli, GI1788550, Length=248, Percent_Identity=50, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1790437, Length=234, Percent_Identity=50, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=40.809968847352, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1790299, Length=245, Percent_Identity=46.1224489795918, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI87082152, Length=324, Percent_Identity=39.5061728395062, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788905, Length=272, Percent_Identity=41.9117647058824, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI87081872, Length=242, Percent_Identity=45.4545454545455, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=43.1297709923664, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.1764705882353, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1789828, Length=266, Percent_Identity=36.0902255639098, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=32.6732673267327, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 74794; Mature: 74663

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSSDPAAEFFLGKFSNLEQLFGQAQFGVSIIDCDLRYIYVNDRMASINGVPPKEHLGK
CCCCCCCHHHHHHHHHCCHHHHHCHHHCCCEEEEECEEEEEEECCEECCCCCCCHHHHHH
TVREVDRFVALVVEPLLNRTIHEDHSFVDLEIRLSKPGDPQEKSWLICSYPLKESDGSVT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEE
RCGLVVQDITEQKLRQSLQTERLQVEAFLSNLSSAFINVPVSEVDRKIEKALQKVVNFLG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHC
FERSSIWHFTPDRSSLRITHSYALPGVKPPPTELMNLIPIWIEMIQLGESFRVSDVEELS
CCCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DKFWREKQYCKDQGGIKSIMFIPASVGGTIVGAITFVSYSVKKEWPDELTQRLRLLWEVF
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ANALERKRADQKIQNALAEIRQLKDRLEAENVYLRDQIDVEYKHEEIIGKSEAIRNVLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECHHHHCCHHHHHHHHHHH
IQQVAPTDSTVLILGETGTGKELIARAIHNAGQRKARAMIKVNCAALPAALIEAELFGHE
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHEEEEHHHHHHHHHHHHHHCCC
KGAYTGAVSSQIGRFEAANGSTIFLDEIGELPLELQSKLLRVIQEGQFERLGNSKPVKAD
CCCCCCHHHHHHCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEC
VRVIAATNVSLKQAVKEGKFREDLYYRLNVFPIFVPPLRDRQEDIPLLVWAMVEEFSKVF
EEEEEECCHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GKTIERIPRKNMEIMERYLWPGNIRELRNLVERAMILSNGGTLVVDILDGSAAAANEPMT
HHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
MEAAERKHIGFILEKTGWRIRGKGGAAEILDMKPTTLHSRIKKLGVKRKNGTLEPPPAAE
HHHHHHHHCCEEEECCCCEEECCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
KPTS
CCCC
>Mature Secondary Structure 
ALSSDPAAEFFLGKFSNLEQLFGQAQFGVSIIDCDLRYIYVNDRMASINGVPPKEHLGK
CCCCCCHHHHHHHHHCCHHHHHCHHHCCCEEEEECEEEEEEECCEECCCCCCCHHHHHH
TVREVDRFVALVVEPLLNRTIHEDHSFVDLEIRLSKPGDPQEKSWLICSYPLKESDGSVT
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEE
RCGLVVQDITEQKLRQSLQTERLQVEAFLSNLSSAFINVPVSEVDRKIEKALQKVVNFLG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHC
FERSSIWHFTPDRSSLRITHSYALPGVKPPPTELMNLIPIWIEMIQLGESFRVSDVEELS
CCCCCEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
DKFWREKQYCKDQGGIKSIMFIPASVGGTIVGAITFVSYSVKKEWPDELTQRLRLLWEVF
HHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ANALERKRADQKIQNALAEIRQLKDRLEAENVYLRDQIDVEYKHEEIIGKSEAIRNVLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEECHHHHCCHHHHHHHHHHH
IQQVAPTDSTVLILGETGTGKELIARAIHNAGQRKARAMIKVNCAALPAALIEAELFGHE
HHHHCCCCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHEEEEHHHHHHHHHHHHHHCCC
KGAYTGAVSSQIGRFEAANGSTIFLDEIGELPLELQSKLLRVIQEGQFERLGNSKPVKAD
CCCCCCHHHHHHCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCEEC
VRVIAATNVSLKQAVKEGKFREDLYYRLNVFPIFVPPLRDRQEDIPLLVWAMVEEFSKVF
EEEEEECCHHHHHHHHCCCCHHHHEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GKTIERIPRKNMEIMERYLWPGNIRELRNLVERAMILSNGGTLVVDILDGSAAAANEPMT
HHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
MEAAERKHIGFILEKTGWRIRGKGGAAEILDMKPTTLHSRIKKLGVKRKNGTLEPPPAAE
HHHHHHHHCCEEEECCCCEEECCCCCEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
KPTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]