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The map label for this gene is nusA [H]

Identifier: 197117688

GI number: 197117688

Start: 1501944

End: 1503095

Strand: Direct

Name: nusA [H]

Synonym: Gbem_1300

Alternate gene names: 197117688

Gene position: 1501944-1503095 (Clockwise)

Preceding gene: 197117687

Following gene: 197117689

Centisome position: 32.54

GC content: 63.45

Gene sequence:

>1152_bases
GTGGAAACAAGCTTTAACCTCAAGCACACGATCGACCAGATCGTAAAAGAGAAGGGGATCGATAAGAGCATTGTAGTGGA
GGCGCTGGAGCAGGCTGTCCTCACCGCTGCCAACAAGAAGTTTCGCAATACCCGCGACCTTGAGGCGCACTACAATCCCG
AGGAAGGCGAGGTGGAGCTGTTCGAATTCGTTACCGTCGTGGAAGAGGTGCAGGATTCCTATCGCGAGATTGAGCTGGAC
GAGGCGCGCGAGGAGGATCCTGAGGTAGAGATCGGCGACTCCATCGGCATGAAGATGGACGCTTCCGGCTTCTCCAGGAT
CGCCGCCCAGACCGCAAAGCAGGTGATCATCCAGCGCGTGCGCGAGGCCGAGCGCGAGACCATCTTCAACGAGTTCATGG
AGCGCCAGGGGGAGATCGTCAACGGCGTGGTGCGCCGCTTCGAGAAGGGTGACCTGATCGTCGACCTGGGGCGCGCCGAG
GCGCTTTTGCCGCACAAGGAGCAGGCCCCGCGCGAGGTCTACCGTCAGGGGGACCGCGTCAAGGCGCTCATCACCGAGAT
CCGCATGACCACCAAGGGGCCGCAGATCATGCTCTCCCGCACCCATCCCACCATGCTCGCCAAGCTTTTCGAAGCGGAGG
TGCCGGAAATCGCGGAGGGGATCGTCGAGATCAAGAGCGTGGTGCGCGAGCCGGGCGGGCGCGCCAAGATCGCCGTCTAC
TCGCATGACTCCGACGTCGACCCGGTCGGCGCCTGCGTCGGCATGAGGGGGTCCCGCGTGCAGAACGTGGTCTCCGAGCT
GCGCGGCGAGAAGATCGACATCATCCCCTGGAGCGAGGATATCGCCCGCTTCGCCTGCAACGCCCTGGCTCCCGCCGTGG
TGACCAAGGTGTACGTCGATGAAGAGGAGTACGCCATGGAGGTGATCGTGGCGGACGACCAGCTTTCGCTTGCCATCGGC
AAGCGCGGCCAGAACGTGCGTCTGGCGGCGAAGCTTACCGGCTGGAAGATCGACATCAAGAGCGAGACCCGCATGGCCGA
GGCCGAGCTGCAGCAGTTTGCCTCCTACGACGGCACCGAGGTGGAAGAGGAGCCGGCCGAAGAGGCGACGGAAGCCCAGC
CGCAGGTCGAGGCCGCCGAAGAGGAGATCTAG

Upstream 100 bases:

>100_bases
GGGCAAAAGGCCCGCGTCCCTTTGGACAAGGTGGCCAAGGCGAACCTGGAATTCGAATTCTAAAAATTGACTATACCTAG
CAAGTGAAGGAGACCGTGAC

Downstream 100 bases:

>100_bases
CGCAGCATGCCCAAGGCCAACCCGCAAAGAAGCTGCCTCGCCTGCCGTGAGACCAAGGACAAGGGGAGCCTGCTTCGCCT
GGTGCTGGCCCCGGACGGCA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
METSFNLKHTIDQIVKEKGIDKSIVVEALEQAVLTAANKKFRNTRDLEAHYNPEEGEVELFEFVTVVEEVQDSYREIELD
EAREEDPEVEIGDSIGMKMDASGFSRIAAQTAKQVIIQRVREAERETIFNEFMERQGEIVNGVVRRFEKGDLIVDLGRAE
ALLPHKEQAPREVYRQGDRVKALITEIRMTTKGPQIMLSRTHPTMLAKLFEAEVPEIAEGIVEIKSVVREPGGRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDIARFACNALAPAVVTKVYVDEEEYAMEVIVADDQLSLAIG
KRGQNVRLAAKLTGWKIDIKSETRMAEAELQQFASYDGTEVEEEPAEEATEAQPQVEAAEEEI

Sequences:

>Translated_383_residues
METSFNLKHTIDQIVKEKGIDKSIVVEALEQAVLTAANKKFRNTRDLEAHYNPEEGEVELFEFVTVVEEVQDSYREIELD
EAREEDPEVEIGDSIGMKMDASGFSRIAAQTAKQVIIQRVREAERETIFNEFMERQGEIVNGVVRRFEKGDLIVDLGRAE
ALLPHKEQAPREVYRQGDRVKALITEIRMTTKGPQIMLSRTHPTMLAKLFEAEVPEIAEGIVEIKSVVREPGGRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDIARFACNALAPAVVTKVYVDEEEYAMEVIVADDQLSLAIG
KRGQNVRLAAKLTGWKIDIKSETRMAEAELQQFASYDGTEVEEEPAEEATEAQPQVEAAEEEI
>Mature_383_residues
METSFNLKHTIDQIVKEKGIDKSIVVEALEQAVLTAANKKFRNTRDLEAHYNPEEGEVELFEFVTVVEEVQDSYREIELD
EAREEDPEVEIGDSIGMKMDASGFSRIAAQTAKQVIIQRVREAERETIFNEFMERQGEIVNGVVRRFEKGDLIVDLGRAE
ALLPHKEQAPREVYRQGDRVKALITEIRMTTKGPQIMLSRTHPTMLAKLFEAEVPEIAEGIVEIKSVVREPGGRAKIAVY
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDIARFACNALAPAVVTKVYVDEEEYAMEVIVADDQLSLAIG
KRGQNVRLAAKLTGWKIDIKSETRMAEAELQQFASYDGTEVEEEPAEEATEAQPQVEAAEEEI

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=354, Percent_Identity=41.8079096045198, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42917; Mature: 42917

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSFNLKHTIDQIVKEKGIDKSIVVEALEQAVLTAANKKFRNTRDLEAHYNPEEGEVEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FEFVTVVEEVQDSYREIELDEAREEDPEVEIGDSIGMKMDASGFSRIAAQTAKQVIIQRV
HHHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHHHHH
REAERETIFNEFMERQGEIVNGVVRRFEKGDLIVDLGRAEALLPHKEQAPREVYRQGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCCCCCHHHHHHCCCHH
KALITEIRMTTKGPQIMLSRTHPTMLAKLFEAEVPEIAEGIVEIKSVVREPGGRAKIAVY
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDIARFACNALAPAVVTKVYVD
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHEEC
EEEYAMEVIVADDQLSLAIGKRGQNVRLAAKLTGWKIDIKSETRMAEAELQQFASYDGTE
CHHHEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHCCCCCC
VEEEPAEEATEAQPQVEAAEEEI
CCCCHHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
METSFNLKHTIDQIVKEKGIDKSIVVEALEQAVLTAANKKFRNTRDLEAHYNPEEGEVEL
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
FEFVTVVEEVQDSYREIELDEAREEDPEVEIGDSIGMKMDASGFSRIAAQTAKQVIIQRV
HHHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCCCCCEECCHHHHHHHHHHHHHHHHHHH
REAERETIFNEFMERQGEIVNGVVRRFEKGDLIVDLGRAEALLPHKEQAPREVYRQGDRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHCCCCCCCCHHHHHHCCCHH
KALITEIRMTTKGPQIMLSRTHPTMLAKLFEAEVPEIAEGIVEIKSVVREPGGRAKIAVY
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
SHDSDVDPVGACVGMRGSRVQNVVSELRGEKIDIIPWSEDIARFACNALAPAVVTKVYVD
ECCCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHEEC
EEEYAMEVIVADDQLSLAIGKRGQNVRLAAKLTGWKIDIKSETRMAEAELQQFASYDGTE
CHHHEEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHCCCCCC
VEEEPAEEATEAQPQVEAAEEEI
CCCCHHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]